scholarly journals Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Chi Zhang ◽  
Li Li ◽  
Shiping Cheng ◽  
Debajyoti Chowdhury ◽  
Yong Tan ◽  
...  
Keyword(s):  
Pathway Analysis ◽  
Complement C3 ◽  
Integrative Approach ◽  
Weight Changes ◽  
Serum Samples ◽  
Phosphorylated Proteins ◽  
Circulating Markers ◽  
One Year ◽  
Study Participants

Abstract Background Hypertension (HTN) patients who have phlegm-dampness syndrome (PDS) tend to be obese and have worse outcomes. However, the association of body weight (BW) changes and mechanisms underlying the pathophysiology of HTN-PDS are not well elucidated. This study aims to identify the longitudinal observations associated with the circulating markers discriminating BW changes of individuals with HTN-PDS. Methods An integrative approach relying on metabolomics and proteomics was applied to serum samples from HTN-PDS patients in a prospective cohort to identify the plausible mechanistic pathways underpinning HTN-PDS pathophysiology. Study participants were determined to have experienced a weight change if they showed a 5%–15% increase/reduction in BW at the end of the follow-up period. The joint pathway analysis and network analysis were performed using Ingenuity Pathway Analysis (IPA®) on the serum samples obtained from the participants over the period. Results The study involved 22 HTN-PDS patients who were overweight initially and were able to lose enough weight and 24 HTN-PDS individuals who developed overweight from normal BMI during a one-year follow-up. Our analysis suggested three types of phosphatidylcholine (PC) were altered. PC (22:2(13Z,16Z)/24:1(15Z)) and LysoPC (16:1(9Z)) were decreased in Queryweight gain samples, whereas the levels of PC (14:0/16:0) were increased in weight loss samples. The metabolomic analysis suggested 24 metabolites associated with HTN-PDS. Of them, 13 were up-regulated and 11 were down-regulated. The two-dimensional difference gel electrophoresis (2D DIGE) identified 45 phosphorylated proteins got altered in the HTN-PDS patients, wherein 23 were up-regulated and 22 were down-regulated. Integrated proteomic and metabolomics analyse acknowledged biomarkers PC, Complement C3, C4a/C4b, A2M and SERPINF1 as strong predictors for BW changes in HTN-PDS patients. Conclusion The combined serum proteomic and metabolomic profiling reveals a link between BW change and the complement system activity, altered phosphatidylcholine metabolism in HTN-PDS patients. Future studies with larger cohorts are required to strengthen and validate these findings.

Depression among Elderly Residents of Local-Authority Residential Homes

1990 ◽  
Vol 156 (5) ◽  
pp. 667-675 ◽  
Author(s):  
David Ames
Keyword(s):  
Local Authority ◽  
Social Activity ◽  
The Elderly ◽  
High Rate ◽  
Homes For The Elderly ◽  
Organic Mental Disorder ◽  
Psychiatric Intervention ◽  
One Year ◽  
Study Participants

Of 390 residents in 12 local-authority homes for the elderly, 93 had evidence of depression on screening and underwent standard clinical assessment. Half had an affective disorder, and a further third had depressive symptoms in the setting of an organic mental disorder. The 93 residents had a high rate of physical illness and disability, undertook little social activity, and were visited infrequently. A range of interventions for depressed residents, the majority social in type, mostly proved difficult to implement. There was no evidence of efficacy of psychiatric intervention at three months; at one-year follow-up a quarter of study participants had died, and 28% of those who were reassessed for depression showed evidence of recovery.

ACUTE CASES OF SYDENHAM'S CHOREA SOUGHT

PEDIATRICS ◽  
1989 ◽  
Vol 83 (6) ◽  
pp. 1054-1054
Keyword(s):  
Mental Health ◽  
Child Psychiatry ◽  
Serum Samples ◽  
Obsessive Compulsive ◽  
Obsessive Compulsive Symptoms ◽  
Sydenham’S Chorea ◽  
Sydenham's Chorea ◽  
One Year

The Child Psychiatry Branch of the National Institute of Mental Health is seeking patients for a study of obsessive compulsive symptoms accompanying Sydenham's chorea. Eligible patients should have had recent (within 2 months) onset of Sydenham's chorea, continue to have choreic symptoms and be at least 6 years of age. This study will rate obsessive compulsive symptoms and link these symptoms to anti-CNS autoantibodies. Patients will be interviewed by phone or in person (out of town subjects will be asked to travel at our expense to the NIH in Bethesda). Brief follow-up interviews will be conducted every 2 months for one year. Serum samples (5 cc) will be obtained on four separate occasions. There will be no expense to the patient and no remuneration. Please call (301) 496-6081 or write: Dr Susan E. Swedo or Dr Judith L. Rapoport; Child Psychiatry Branch, NIMH, Bldg 10, Room 6N240, 9000 Rockville Pike, Bethesda, MD 20892.

scholarly journals Preliminary Report of Encephalitis Surveillance Study in Rajshahi Medical College Hospital

1970 ◽  
Vol 17 (2) ◽  
pp. 75-79
Author(s):  
Sultana M Hussain ◽  
MJ Hossain ◽  
ARMS Ekram ◽  
ES Gurley ◽  
MM Alam ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Nipah Virus ◽  
Medical College Hospital ◽  
Serum Samples ◽  
Recent Infection ◽  
College Hospital ◽  
Medical College ◽  
Control And Prevention ◽  
One Year

More than 100 different viruses, bacteria, toxins and drugs can cause acute encephalitis. A prospective, hospital-based study to define the causes of encephalitis in Bangladesh began in June 2003. At Rajshahi Medical College Hospital in one year 105 out of 391 suspected encephalitis patients were enrolled. The specimens were collected from these patients and were tested for a variety of pathogens at twelve laboratories at the Centers for Disease Control and Prevention (CDC) in Atlanta and Ft. Collins, USA. Among the first 105 patients enrolled, the median age of the patient was 18 years; 55% were male. Twenty-four percent (n-25) died in hospital or before completing the follow-up. Thirteen out of 105 (12.38%) patients had Japanese encephalitis; three of the encephalitis patients had a positive test result of having recent infection with dengue virus and one had encephalitis due to alpha virus, another patient had encephalitis due to echovirus. Three (2.8%) were polymerase chain reaction (PCR) or culture positive for Streptococcus pneumoniae, and 4 for Mycoplasma pneumoniae. No Nipah virus infection was identified among the first 105 serum samples tested. These data suggest that Japanese encephalitis virus is an emerging cause of encephalitis in northern part of Bangladesh. doi: 10.3329/taj.v17i2.3449 TAJ 2004; 17(2): 75-79  

scholarly journals Serological Follow-up of Patients with Erythema Migrans: Persistence of Antibody Response to Borrelia Burgdorferi in Lyme Disease Patients

2003 ◽  
Vol 1 (2) ◽  
pp. 73-77
Author(s):  
I. Christova ◽  
R. Komitova
Keyword(s):  
Lyme Disease ◽  
Antibody Response ◽  
Treatment Failure ◽  
Lyme Borreliosis ◽  
Erythema Migrans ◽  
Serum Samples ◽  
Laboratory Examinations ◽  
One Year ◽  
Antibody Kinetics

The rate of seroconversion before treatment and antibody kinetics after treatment were analyzed and possible interpretations of serologic findings was proposed. Serum samples from 219 patients with Erythema migrans were tested by ELISA for antibodies against B. burgdorferi. Twenty-eight (28 %) to 55 % of the patients showed isolated IgM antibody response, 3–5 % showed isolated IgG response, 6–16 % showed concomitant IgM and IgG responses, and 24–63 % tested seronegative depending on number of days passed after the onset of Lyme borreliosis. One year after treatment, 38 % of the patients still had IgG response and 10% had IgM antibodies against B. burgdorferi. Furthermore, 4 of 106 seronegative patients revealed IgM response three months after treatment despite lack of signs or symptoms of active Lyme borreliosis. We concluded that persistence of antibody response is not indicative of treatment failure, although regular clinical and laboratory examinations, including PCR, should follow successful treatment.

scholarly journals Long-lasting humoral immunity in Covid-19 infected patients at a University Hospital Clinic in Östergötland County Council during 2020-2021

2021 ◽  
Author(s):  
M Azharuddin ◽  
D Aili ◽  
R Selegård ◽  
Sajjad Naeimipour ◽  
M Sunnerhagen ◽  
...  
Keyword(s):  
County Council ◽  
University Hospital ◽  
Spike Protein ◽  
Test Results ◽  
Structural Protein ◽  
Serum Samples ◽  
Serum Igg ◽  
Nasal Swabs ◽  
Study Participants

AbstractLongitudinal serum samples and nasopharyngeal/nasal swab samples were collected from forty-eight individuals (median age 66yrs) with Covid-19 PCR-positive test results at Linköping University Hospital. Samples were collected from initial visit and for 6 months follow up. Presence of serum IgG and IgA against SARS-CoV-2 antigens (S1-spike, nucleocapsid and NSP3) were analyzed. Nasal swabs were tested for presence of IgA against the outer envelope S1 spike protein. Ninety-two percent of participants were seropositive against SARS-CoV-2 recombinant proteins at day 28 from study entry and all (100%) were seropositive from samples collected at 2 months or later. The most common antibody responses (both serum IgG, mainly IgG1 and IgA) were detected against the S1-spike protein and the nucleoprotein. In samples collected from nasal tissues considerably lower frequencies of IgA-positive reactivities were detected. Sixteen to 18 percent of study participants showed detectable IgA levels in nasal samples, except at day 60 when 36% of tested individuals showed presence of IgA against the S1-spike protein. The study suggests that the absolute majority of studied naturally infected Covid-19 patient in the Linkoping, Ostergotland health region develop over 6 months lasting detectable levels of serum IgG and IgA responses towards the SARS-CoV-2 S1-spike protein as well as against the nucleoprotein, but not against the non-structural protein 3.

scholarly journals Epithelial Alarmins in Serum and Exhaled Breath in Patients with Idiopathic Pulmonary Fibrosis: A Prospective One-Year Follow-Up Cohort Study

2019 ◽  
Vol 8 (10) ◽  
pp. 1590 ◽  
Author(s):  
Majewski ◽  
Szewczyk ◽  
Białas ◽  
Miłkowska-Dymanowska ◽  
Górski ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Antifibrotic Therapy ◽  
Lung Compartment ◽  
Before And After ◽  
One Year ◽  
Study Participants

Background: Recently, epithelial alarmins have been shown to play important roles in non-allergen driven respiratory diseases like idiopathic pulmonary fibrosis (IPF). Little is known about the expression of the epithelial alarmins in IPF. Methods: This study aimed to prospectively examine interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP) levels in the serum and exhaled breath condensate (EBC) in patients with IPF before and after one-year of antifibrotic treatment. A total of 82 volunteers, including 52 patients diagnosed with IPF that qualified for antifibrotic therapy as well as 30 controls, were examined. All study participants underwent baseline peripheral blood and EBC sampling. In 35 out of 52 IPF subjects, a follow-up sampling was performed after 12 months of antifibrotic treatment. Concentrations of alarmins in the serum and EBC were evaluated by means of ELISA. Results: Baseline TSLP concentrations were significantly elevated in patients with IPF compared to controls both in the serum (p < 0.05) and EBC (p < 0.0001). Baseline IL-25 and IL-33 serum and EBC levels did not differ significantly between IPF subjects and controls. Prospective analysis of changes in the epithelial alarmin levels showed significantly decreased IL-25 and TSLP EBC concentrations after 12 months of antifibrotic treatment (p < 0.05), which was observed in the subgroup of IPF patients treated with pirfenidone, but not in those treated with nintedanib. In stable patients with IPF over a study period (absolute forced vital capacity (FVC) % of predicted decline/year ≤ 5%, n = 25), a significant decrease in the EBC levels of both IL-25 and TSLP after 12 months of antifibrotic treatment was noted (p < 0.05), whereas, in progressor IPF patients (absolute FVC % of predicted decline/year > 5%, n = 10), a significant decrease was noted in the IL-25 EBC levels only (p < 0.05). Conclusions: Elevated TSLP levels in patients with IPF and their significant decrease in the lung compartment during antifibrotic therapy in stable patients with IPF, but not in progressors, support its significant contribution to pro-fibrotic type 2 immune responses in IPF. Noted changes in the epithelial alarmins concentration in the lung compartment during pirfenidone therapy may suggest its possible interaction with epithelial alarmins pathways in IPF.

scholarly journals High secondary attack rate and persistence of SARS-CoV-2 antibodies in household transmission study participants, Finland 2020

2021 ◽  
Author(s):  
Timothee Dub ◽  
Hanna Nohynek ◽  
Lotta Hagberg ◽  
Oona Liedes ◽  
Anu Haveri ◽  
...  
Keyword(s):  
Attack Rate ◽  
Neutralizing Antibodies ◽  
Serum Samples ◽  
Household Transmission ◽  
Transmission Study ◽  
Household Members ◽  
Transmission Studies ◽  
Study Participants ◽  
Index Cases

Background Household transmission studies offer the opportunity to assess both secondary attack rate and persistence of SARS-CoV-2 antibodies over time. Methods We invited confirmed COVID-19 cases and their household members to attend up to four household visits with collection of nasopharyngeal and serum samples over 28 days after index case onset. We calculated secondary attack rates (SAR) based on the presence of SARS-CoV-2 nucleoprotein IgG antibodies (IgG Ab) and/or neutralizing antibodies (NAb) overall and per households. Three and six months later, we assessed the persistence of SARS-CoV-2 antibodies. Findings We recruited 39 index cases and 90 household members. Among 87 household members evaluated, SAR was 48% (n=42), including 37 symptomatic secondary cases. In total, 80/129 (62%) participants developed both IgG Ab and NAb, while three participants only developed IgG Ab. Among participants who had both IgG Ab and NAb during the initial follow-up, 68/69 (99%) and 63/70 (90%) had IgG Ab and NAb at 3 months, while at 6 months, 59/75 (79%) and 63/75 (84%) had IgG Ab and NAb, respectively. Participants who required hospital care had initially 5-fold IgG Ab concentrations compared to cases with mild symptoms and 8-fold compared to asymptomatic cases. Interpretation Following detection of a COVID-19 case in a household, other members had a high risk of becoming infected. Follow-up of participants showed strong persistence of antibodies in most cases. Funding This study was supported by THL coordinated funding for COVID-19 research (Finnish Government's supplementary budget) and by the Academy of Finland (Decision number 336431).

scholarly journals Barodontalgia among Aircrew of Bangladesh Air Force

2020 ◽  
Vol 15 (2) ◽  
pp. 186-188
Author(s):  
Md Masudur Rahman ◽  
Mizanur Rahman Khan ◽  
Mohammad Ahmed Ahsan ◽  
Mohammad Khairul Alam ◽  
Md Abdul Wahab
Keyword(s):  
Air Force ◽  
Atmospheric Pressure ◽  
Cross Sectional Study ◽  
Moderate Pain ◽  
Sectional Study ◽  
Cross Sectional ◽  
One Year ◽  
Pressure Changes ◽  
Study Participants

subjected to atmospheric pressure changes and have an influence on flights. Barodontalgia is caused whenever the pulp is exposed to differential pressure gradient. Objective:  To see the barodontalgia among aircrew of Bangladesh Air Force. Methodology: A cross-sectional study was carried out in Dental wing medical squadron BAF base Basher Dhaka. The study participants were recruited during one year among all the pilots and crewmembers attending departments specializing in medical follow-up at the study period from the Bangladesh Air Force. Results: Among the 50 barodontalgia group, most 39(78%) of the respondents were found technical and military flying whereas 50 without barodontalgia group 38(76%) specifically in military flying. In barodontalgia, 62% respondents were found in moderate pain (4-6), 26% in mild pain (1-3) and 32% in severe pain (7-10). In barodontalgia, 50% respondents were descent of barodontalgia, 52% were altitude where barodontalgia was reported to appear during the flight was <4000 meters and 70% were flight speed was 500-1000 km/h. Most of the respondents were found specifically in commercial flights in patients with barodontalgia. Majority pain intensity was found moderately in barodontalgia JAFMC Bangladesh. Vol 15, No 2 (December) 2019: 186-188

1848: Revolix 70 Watt 2 Micron Continuous Wave Laser Vaporesection of the Prostate. Preliminary Results After a One Year Follow-Up

2007 ◽  
Vol 177 (4S) ◽  
pp. 614-614
Author(s):  
Thorsten Bach ◽  
Thomas R.W. Herrmann ◽  
Roman Ganzer ◽  
Andreas J. Gross
Keyword(s):  
Continuous Wave ◽  
Continuous Wave Laser ◽  
Preliminary Results ◽  
Wave Laser ◽  
One Year

337: Monarc™ Transobturator Sling for the Treatment of Stress Urinary Incontinence: A Prospective, Multi­Center Study with One Year Follow-Up

2006 ◽  
Vol 175 (4S) ◽  
pp. 110-110 ◽  
Author(s):  
Robert D. Moore ◽  
John Miklos ◽  
L. Dean Knoll ◽  
Mary Dupont ◽  
Mickey Karram ◽  
...  
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Transobturator Sling ◽  
One Year
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