scholarly journals Genome-based classification of Burkholderia cepacia complex provides new insight into its taxonomic status

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Yuan Jin ◽  
Jianglin Zhou ◽  
Jing Zhou ◽  
Mingda Hu ◽  
Qi Zhang ◽  
...  
1979 ◽  
Vol 57 (10) ◽  
pp. 1998-2009 ◽  
Author(s):  
Jacquie McGlade ◽  
Hugh MacCrimmon

The degree of taxonomic congruence between three methods of classification is examined for three Quebec populations of wild brook trout, Salvelinus fontinalis, from the Matamek–Moisie watersheds. The three methods utilize morphometric, meristic, and electrophoretic characters. Electrophoretic comparison is also made between the Quebec samples and brook trout from the Dorion Hatchery, Ontario. Using discriminant function analysis on data for the three Quebec populations, a subset of nine morphometric measurements was identified with between 84.0 and 92.6% correct classification, and a meristic subset of four characters, including mandibular pores, with between 72.5 and 95.0% correct classification. Electrophoretic analysis has identified a number of newly defined alleles at four loci, from five tissues. The meristic and electrophoretic analyses show congruence in the order of similarity and, therefore, are considered the most suitable methods for classification of the brook trout. Morphometric analysis provides an insight into localized adaptation particularly in response to habitat and resource utilization. An understanding of the taxonomic status of populations of brook trout requires not only a definition of individuals in terms of meristic and electrophoretic profiles, but also morphological characterization, to increase our understanding of their ecology and response to other faunal components.


2019 ◽  
Vol 62 (9) ◽  
pp. 3265-3275
Author(s):  
Heather L. Ramsdell-Hudock ◽  
Anne S. Warlaumont ◽  
Lindsey E. Foss ◽  
Candice Perry

Purpose To better enable communication among researchers, clinicians, and caregivers, we aimed to assess how untrained listeners classify early infant vocalization types in comparison to terms currently used by researchers and clinicians. Method Listeners were caregivers with no prior formal education in speech and language development. A 1st group of listeners reported on clinician/researcher-classified vowel, squeal, growl, raspberry, whisper, laugh, and cry vocalizations obtained from archived video/audio recordings of 10 infants from 4 through 12 months of age. A list of commonly used terms was generated based on listener responses and the standard research terminology. A 2nd group of listeners was presented with the same vocalizations and asked to select terms from the list that they thought best described the sounds. Results Classifications of the vocalizations by listeners largely overlapped with published categorical descriptors and yielded additional insight into alternate terms commonly used. The biggest discrepancies were found for the vowel category. Conclusion Prior research has shown that caregivers are accurate in identifying canonical babbling, a major prelinguistic vocalization milestone occurring at about 6–7 months of age. This indicates that caregivers are also well attuned to even earlier emerging vocalization types. This supports the value of continuing basic and clinical research on the vocal types infants produce in the 1st months of life and on their potential diagnostic utility, and may also help improve communication between speech-language pathologists and families.


Water ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 56
Author(s):  
Martina Miloloža ◽  
Dajana Kučić Grgić ◽  
Tomislav Bolanča ◽  
Šime Ukić ◽  
Matija Cvetnić ◽  
...  

High living standards and a comfortable modern way of life are related to an increased usage of various plastic products, yielding eventually the generation of an increased amount of plastic debris in the environment. A special concern is on microplastics (MPs), recently classified as contaminants of emerging concern (CECs). This review focuses on MPs’ adverse effects on the environment based on their bioactivity. Hence, the main topic covered is MPs’ ecotoxicity on various aquatic (micro)organisms such as bacteria, algae, daphnids, and fish. The cumulative toxic effects caused by MPs and adsorbed organic/inorganic pollutants are presented and critically discussed. Since MPs’ bioactivity, including ecotoxicity, is strongly influenced by their properties (e.g., types, size, shapes), the most common classification of MPs types present in freshwater are provided, along with their main characteristics. The review includes also the sources of MPs discharge in the environment and the currently available characterization methods for monitoring MPs, including identification and quantification, to obtain a broader insight into the complex problem caused by the presence of MPs in the environment.


2020 ◽  
Vol 9 (1) ◽  
pp. 51
Author(s):  
Min Yi Wong ◽  
Yuan-Hsi Tseng ◽  
Tsung-Yu Huang ◽  
Bor-Shyh Lin ◽  
Chun-Wu Tung ◽  
...  

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.


2013 ◽  
Vol 41 (11) ◽  
pp. 1038-1042 ◽  
Author(s):  
Maria Beatriz Souza Dias ◽  
Larissa G.T. Cavassin ◽  
Valeska Stempliuk ◽  
Luciene S. Xavier ◽  
Renata D. Lobo ◽  
...  

2013 ◽  
Vol 31 (10) ◽  
pp. 665-668 ◽  
Author(s):  
Laura Barrado ◽  
M. Teresa Martinez ◽  
Jennifer Villa ◽  
M. Ángeles Orellana ◽  
Esther Viedma ◽  
...  

2010 ◽  
Vol 147 (1) ◽  
pp. 161-187 ◽  
Author(s):  
Jérémy Blanc ◽  
Frédéric Mangolte

AbstractIn this article we study the transitivity of the group of automorphisms of real algebraic surfaces. We characterize real algebraic surfaces with very transitive automorphism groups. We give applications to the classification of real algebraic models of compact surfaces: these applications yield new insight into the geometry of the real locus, proving several surprising facts on this geometry. This geometry can be thought of as a half-way point between the biregular and birational geometries.


2002 ◽  
Vol 70 (5) ◽  
pp. 2715-2720 ◽  
Author(s):  
Karen K. Chu ◽  
Donald J. Davidson ◽  
T. Keith Halsey ◽  
Jacqueline W. Chung ◽  
David P. Speert

ABSTRACT Cystic fibrosis patients infected with strains from different genomovars of the Burkholderia cepacia complex can experience diverse clinical outcomes. To identify genomovar-specific determinants that might be responsible for these differences, we developed a pulmonary model of infection in BALB/c mice. Mice were rendered leukopenic by administration of cyclophosphamide prior to intranasal challenge with 1.6 × 104 bacteria. Five of six genomovar II strains persisted at stable numbers in the lungs until day 16 with minimal toxicity, whereas zero of seven genomovar III strains persisted but resulted in variable toxicity. We have developed a chronic pulmonary model of B. cepacia infection which reveals differences among genomovars in terms of clinical infection outcome.


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