scholarly journals Development of an RNAi-based microalgal larvicide for the control of Aedes aegypti

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Xiaowen Fei ◽  
Yang Zhang ◽  
Lili Ding ◽  
Sha Xiao ◽  
Xiaoqing Xie ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Large Scale ◽  
Population Control ◽  
Small Scale ◽  
Expression Plasmid ◽  
Control Programs ◽  
New Class ◽  
Feeding Experiments ◽  
Environmentally Safe ◽  
Integrated Vector Control

Abstract Background Mosquito-borne diseases affect over half of the human population globally. Multiple studies have shown that chemical insecticides are ineffective because of resistance. Therefore, environmentally safe mosquito population control tools need to be developed. Ribonucleic acid interference (RNAi) is a reverse genetic mechanism recently introduced as a new pest control tool. This technique represents a new class of biorational technology that could combat the increased global incidence of insecticide resistance. The technique has the potential of becoming a critical component of integrated vector control programs. Methods A 3-hydroxykynurenine transaminase (3-HKT) RNAi expression plasmid was constructed, generated and transformed into Chlamydomonas and Chlorella algae. The transgenic algae were then used to feed Ae. aegypti mosquito larvae. The feeding experiments were conducted on a small and large scale with 10 and about 300 larvae, respectively. The mortality rate of the larvae was calculated over 30 days. In addition, histological examination of the insect tissues was performed to examine the extent of tissue damage. Results The integumentary system and midguts of larvae fed with transgenic Chlamydomonas were severely damaged. The mortality rate of the larvae fed with transgenic Chlamydomonas ranged from 60 to 100% in small-scale tests. The survival rate of adult mosquitoes was 0.0% in a large-scale feeding experiment when the larvae were fed with transgenic Chlamydomonas. Moreover, when the larvae were fed with transgenic Chlorella, the mortality rate ranged from 6.7% to 43% compared to that fed wild-type Chlorella. Conclusions 3HKT RNAi transgenic algae are in some scales lethal to Ae. aegypti. The findings of this study indicate that technology based on microalgae RNAi may provide a new way to control mosquito populations. Graphical Abstract

scholarly journals A Field Efficacy Evaluation of In2Care Mosquito Traps in Comparison with Routine Integrated Vector Management at Reducing Aedes aegypti

2021 ◽  
Vol 37 (4) ◽  
pp. 242-249
Author(s):  
Eva A. Buckner ◽  
Katie F. Williams ◽  
Samantha Ramirez ◽  
Constance Darrisaw ◽  
Juliana M. Carrillo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Large Scale ◽  
Pathogen Transmission ◽  
Small Scale ◽  
Integrated Vector Management ◽  
Control Programs ◽  
Vector Management ◽  
Scale Field ◽  
Large Scale Field ◽  
Eggs And Larvae

ABSTRACT Aedes aegypti is the predominant vector of dengue, chikungunya, and Zika viruses. This mosquito is difficult to control with conventional methods due to its container-inhabiting behavior and resistance to insecticides. Autodissemination of pyriproxyfen (PPF), a potent larvicide, has shown promise as an additional tool to control Aedes species in small-scale field trials. However, few large-scale field evaluations have been conducted. We undertook a 6-month-long large-scale field study to compare the effectiveness and operational feasibility of using In2Care Mosquito Traps (In2Care Traps, commercially available Aedes traps with PPF and Beauveria bassiana) compared to an integrated vector management (IVM) strategy consisting of source reduction, larviciding, and adulticiding for controlling Ae. aegypti eggs, larvae, and adults. We found that while the difference between treatments was only statistically significant for eggs and larvae (P < 0.05 for eggs and larvae and P > 0.05 for adults), the use of In2Care Traps alone resulted in 60%, 57%, and 57% fewer eggs, larvae, and adults, respectively, collected from that site compared to the IVM site. However, In2Care Trap deployment and maintenance were more time consuming and labor intensive than the IVM strategy. Thus, using In2Care Traps alone as a control method for large areas (e.g., >20 ha) may be less practical for control programs with the capacity to conduct ground and aerial larviciding and adulticiding. Based on our study results, we conclude that In2Care Traps are effective at suppressing Ae. aegypti and have the most potential for use in areas without sophisticated control programs and within IVM programs to target hotspots with high population levels and/or risk of Aedes-borne pathogen transmission.

Evaluation of a ship-based unoccupied aircraft system (UAS) for surveys of spotted and ribbon seals in the Bering Sea pack ice

2015 ◽  
Vol 3 (3) ◽  
pp. 114-122 ◽  
Author(s):  
Erin E. Moreland ◽  
Michael F. Cameron ◽  
Robyn P. Angliss ◽  
Peter L. Boveng
Keyword(s):  
Bering Sea ◽  
Large Scale ◽  
The Arctic ◽  
Small Scale ◽  
Ecosystem Responses ◽  
New Class ◽  
Pack Ice ◽  
Low Altitude ◽  
Aircraft System ◽  
The Bering Sea

The remote pack ice of the arctic and subarctic seas is challenging to access, yet extremely important to understand and monitor. The pack ice holds the key to understanding ecosystem responses to climate change and is vital habitat for many species including ice-associated seals. Unoccupied aircraft systems (UAS) are a new class of tools that may overcome the traditional challenges associated with expansive offshore surveys. We conducted UAS flights over the pack ice during a spring 2009 National Oceanic and Atmospheric Administration (NOAA) cruise to the Bering Sea to determine whether advances in UAS technology can enable effective large-scale, systematic ship-based surveys for seals in the seasonal ice of the Bering, Beaufort, and Chukchi Seas. A fixed-wing ScanEagle UAS was successfully launched and recovered from the NOAA ship McArthur II to conduct small-scale transect surveys up to 5 nautical miles (M) from the ship's position. More than 27 000 images were collected from 10 flights over the Bering Sea pack ice and seals were identified in 110 of these images. Review of the images indicated a marked reduction in disturbance to seals when compared to images collected from occupied, low-altitude helicopter surveys. These results suggest that large-scale UAS surveys of arctic and subarctic habitat in United States airspace will be possible with improvements in technology, reduced operational costs, and the establishment of inclusive airspace regulations.

scholarly journals Determination of the Parameters of the Trajectory of Movement of a Standard Tool in Multi-Coordinate Machining of Spatially Modified Teeth of Spur Wheels with the Copying Technique

2021 ◽  
Vol 346 ◽  
pp. 01026
Author(s):  
Roman Grubka ◽  
Alexander Mikhaylov ◽  
Irina Petryaeva ◽  
Natalya Pichko ◽  
Larisa Polyakova
Keyword(s):  
Large Scale ◽  
Cutting Tool ◽  
Reference Points ◽  
Small Scale ◽  
Scale Production ◽  
Cnc Machines ◽  
Control Programs ◽  
Standard Tool ◽  
Spatial Trajectory

The article presents a methodology for determining the parameters of the spatial trajectory of movement of a standard cutting tool processing ring gears with spatially modified teeth working by the copying technique. The implementation of the methodology makes it possible to determine the reference points coordinates of the trajectory of movement of the cutting tool in the process of multiple-axis processing of spatially modified teeth of spur wheels on CNC machines. The results obtained in the development of the methodology for determining the trajectory of the cutting tool movement can be further used in the process of performing the technological preparation for the production of spatially modified ring gears, including large-scale ones, in the conditions of single and small-scale production, as well as with choosing a technological equipment, the appointment of cutting modes, as well as in the process of writing control programs for CNC machines.

Climatic Change and Early Population Dynamics in the Southwestern United States

1970 ◽  
Vol 1 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Cynthia Irwin-Williams ◽  
C. Vance Haynes
Keyword(s):  
United States ◽  
Climatic Change ◽  
Large Scale ◽  
Population Control ◽  
Small Scale ◽  
Human Populations ◽  
Southwestern United States ◽  
Human Occupation ◽  
Cultural System ◽  
Early Human

It is becoming increasingly apparent that the pattern of early human occupation of the Southwestern United States was strongly influenced by the major paleoclimatic events of the period 9500 B.C. to A.D. 700. The size of human populations and the distribution of human settlement at both the regional-topographic and large-scale areal level, known from archaeological research, are directly correlated to climatic change documented by the evidence of geology and palynology.The effect of climatic change is felt through the actions and reactions of the economic subsystem and its linkages with other subsystems. These reactions reflect not only the character of the climatic stimulus but also the existing state of the cultural system. Alternate reactions include direct systemic readaptation to the changed environment (through changed technologies, methods of population control, etc.); or small scale or large scale relocation of populations in different local niches, regions, or areas whose character most closely approximates the conditions to which the cultural system was initially adapted.

Small-Scale Science to Large-Scale Profession

2000 ◽  
Vol 45 (4) ◽  
pp. 396-398
Author(s):  
Roger Smith
Keyword(s):  
Large Scale ◽  
Small Scale

Development of Physics Learning Module Based on Guided Inquiry with Light Wave Material in Class XI of Senior High School

2020 ◽  
Vol 1 (1) ◽  
pp. 1-10
Author(s):  
Evi Rahmawati ◽  
Irnin Agustina Dwi Astuti ◽  
N Nurhayati
Keyword(s):  
Large Scale ◽  
Learning Strategy ◽  
Student Response ◽  
Average Score ◽  
Small Scale ◽  
Design Development ◽  
Teacher Response ◽  
Student Responses ◽  
Analysis Design ◽  
Scientific Skills

IPA Integrated is a place for students to study themselves and the surrounding environment applied in daily life. Integrated IPA Learning provides a direct experience to students through the use and development of scientific skills and attitudes. The importance of integrated IPA requires to pack learning well, integrated IPA integration with the preparation of modules combined with learning strategy can maximize the learning process in school. In SMP 209 Jakarta, the value of the integrated IPA is obtained from 34 students there are 10 students completed and 24 students are not complete because they get the value below the KKM of 68. This research is a development study with the development model of ADDIE (Analysis, Design, Development, Implementation, and Evaluation). The use of KPS-based integrated IPA modules (Science Process sSkills) on the theme of rainbow phenomenon obtained by media expert validation results with an average score of 84.38%, average material expert 82.18%, average linguist 75.37%. So the average of all aspects obtained by 80.55% is worth using and tested to students. The results of the teacher response obtained 88.69% value with excellent criteria. Student responses on a small scale acquired an average score of 85.19% with highly agreed criteria and on the large-scale student response gained a yield of 86.44% with very agreed criteria. So the module can be concluded receiving a good response by the teacher and students.

Planetary gentrification and urban (re)development

2019 ◽  
Vol 61 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Loretta Lees
Keyword(s):  
Low Income ◽  
Policy Making ◽  
Large Scale ◽  
Urban Redevelopment ◽  
Urban Transformation ◽  
Small Scale ◽  
Income Groups ◽  
Low Income Groups

Abstract Gentrification is no-longer, if it ever was, a small scale process of urban transformation. Gentrification globally is more often practised as large scale urban redevelopment. It is state-led or state-induced. The results are clear – the displacement and disenfranchisement of low income groups in favour of wealthier in-movers. So, why has gentrification come to dominate policy making worldwide and what can be done about it?

AGRICULTURAL PRODUCTION OF CA DONG PEOPLE IN RESETTLEMENT AREA OF TRANH RIVER HYDROPOWER NUMBER 2: CURRENT SITUATION AND IMPACT FACTORS

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Bùi Thị Bích Lan
Keyword(s):  
Social Relations ◽  
Agricultural Production ◽  
Large Scale ◽  
Transition Process ◽  
Impact Factors ◽  
Small Scale ◽  
Scale Production ◽  
Technical Application ◽  
Production Models ◽  
Role Of The State

In Vietnam, the construction of hydropower projects has contributed significantly in the cause of industrialization and modernization of the country. The place where hydropower projects are built is mostly inhabited by ethnic minorities - communities that rely primarily on land, a very important source of livelihood security. In the context of the lack of common productive land in resettlement areas, the orientation for agricultural production is to promote indigenous knowledge combined with increasing scientific and technical application; shifting from small-scale production practices to large-scale commodity production. However, the research results of this article show that many obstacles in the transition process are being posed such as limitations on natural resources, traditional production thinking or the suitability and effectiveness of scientific - technical application models. When agricultural production does not ensure food security, a number of implications for people’s lives are increasingly evident, such as poverty, preserving cultural identity, social relations and resource protection. Since then, it has set the role of the State in researching and building appropriate agricultural production models to exploit local strengths and ensure sustainability.

Managing the impacts of mining on Ghana’s water resources from a legal perspective

2018 ◽  
Vol 1 (3) ◽  
pp. 156-165 ◽  
Author(s):  
Nasirudeen Abdul Fatawu
Keyword(s):  
Surface Water ◽  
Water Resources ◽  
Environmental Protection Agency ◽  
Large Scale ◽  
Small Scale ◽  
Mining Activities ◽  
Protection Agency ◽  
Environmental Laws ◽  
Mining Areas ◽  
Legal Perspective

Recent floods in Ghana are largely blamed on mining activities. Not only are lives lost through these floods, farms andproperties are destroyed as a result. Water resources are diverted, polluted and impounded upon by both large-scale minersand small-scale miners. Although these activities are largely blamed on behavioural attitudes that need to be changed, thereare legal dimensions that should be addressed as well. Coincidentally, a great proportion of the water resources of Ghana arewithin these mining areas thus the continual pollution of these surface water sources is a serious threat to the environmentand the development of the country as a whole. The environmental laws need to be oriented properly with adequate sanctionsto tackle the impacts mining has on water resources. The Environmental Impact Assessment (EIA) procedure needs to bestreamlined and undertaken by the Environmental Protection Agency (EPA) and not the company itself.

RANCANG BANGUN PERANGKAT EKSPERIMENTASI PROSES PIROLISIS BIOMASA GELOMBANG MIKRO

2013 ◽  
Vol 14 (2) ◽  
Author(s):  
Noor Fachrizal
Keyword(s):  
Large Scale ◽  
Continuous Model ◽  
Biomass Energy ◽  
Scale Model ◽  
Small Scale ◽  
Laboratory Apparatus ◽  
Liquid Form ◽  
Large Scale Model ◽  
Bio Oil ◽  
Pyrolysis Of Biomass

Biomass such as agriculture waste and urban waste are enormous potency as energy resources instead of enviromental problem. organic waste can be converted into energy in the form of liquid fuel, solid, and syngas by using of pyrolysis technique. Pyrolysis process can yield higher liquid form when the process can be drifted into fast and flash response. It can be solved by using microwave heating method. This research is started from developing an experimentation laboratory apparatus of microwave-assisted pyrolysis of biomass energy conversion system, and conducting preliminary experiments for gaining the proof that this method can be established for driving the process properly and safely. Modifying commercial oven into laboratory apparatus has been done, it works safely, and initial experiments have been carried out, process yields bio-oil and charcoal shortly, several parameters are achieved. Some further experiments are still needed for more detail parameters. Theresults may be used to design small-scale continuous model of productionsystem, which then can be developed into large-scale model that applicable for comercial use.

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