scholarly journals Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Eva Wattrang ◽  
Helena Eriksson ◽  
Tomas Jinnerot ◽  
Maria Persson ◽  
Elisabeth Bagge ◽  
...  
Keyword(s):  
Immune Responses ◽  
Acute Phase ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Erysipelothrix Rhusiopathiae ◽  
Naive Group ◽  
Mannose Binding ◽  
Leukocyte Counts ◽  
Blood Leukocyte ◽  
Binding Lectin

Abstract Erysipelas, a disease caused by Erysipelothrix rhusiopathiae (ER), is an increasing problem in laying hens housed in cage-free systems. This study aimed to monitor immune responses during ER infection of naïve chickens and chickens vaccinated intra muscularly with a commercial inactivated ER vaccine. Chickens were infected intra muscularly with ER at 30 days of age and blood leukocyte counts, serum levels of mannose binding lectin (MBL) and ER-specific IgY were monitored until the experiment was terminated at day 15 after infection. ER was detected in blood from more chickens and at higher bacterial counts in the naïve group (day 1: 1 of 7 chickens; day 3: 6 of 6 chickens) than in the vaccinated group (day 1: 0 of 7 chickens; day 3: 1 of 6 chickens). During the acute phase of infection transient increases in circulating heterophil numbers and serum MBL levels were detected in all ER infected chickens but these responses were prolonged in chickens from the naïve group compared to vaccinated chickens. Before infection IgY titers to ER in vaccinated chickens did not differ significantly from those of naïve chickens but vaccinated chickens showed significantly increased IgY titers to ER earlier after infection compared to chickens in the naïve group. In conclusion, the ER infection elicited prompt acute innate responses in all chickens. Vaccinated chickens did not have high IgY titers to ER prior to infection but did however show lower levels of bacteraemia and their acute immune responses were of shorter duration.

scholarly journals Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection

2005 ◽  
Vol 191 (10) ◽  
pp. 1697-1704 ◽  
Author(s):  
W. K. Eddie Ip ◽  
Kwok Hung Chan ◽  
Helen K. W. Law ◽  
Gloria H. W. Tso ◽  
Eric K. P. Kong ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
In Vitro Assays ◽  
Calcium Dependent ◽  
Control Subjects ◽  
Mannose Binding ◽  
Carbohydrate Recognition Domains ◽  
Binding Lectin

Abstract Little is known about the innate immune response to severe acute respiratory syndrome (SARS) coronavirus (CoV) infection. Mannose-binding lectin (MBL), a key molecule in innate immunity, functions as an ante-antibody before the specific antibody response. Here, we describe a case-control study that included 569 patients with SARS and 1188 control subjects and used in vitro assays to investigate the role that MBL plays in SARS-CoV infection. The distribution of MBL gene polymorphisms was significantly different between patients with SARS and control subjects, with a higher frequency of haplotypes associated with low or deficient serum levels of MBL in patients with SARS than in control subjects. Serum levels of MBL were also significantly lower in patients with SARS than in control subjects. There was, however, no association between MBL genotypes, which are associated with low or deficient serum levels of MBL, and mortality related to SARS. MBL could bind SARS-CoV in a dose- and calcium-dependent and mannan-inhibitable fashion in vitro, suggesting that binding is through the carbohydrate recognition domains of MBL. Furthermore, deposition of complement C4 on SARS-CoV was enhanced by MBL. Inhibition of the infectivity of SARS-CoV by MBL in fetal rhesus kidney cells (FRhK-4) was also observed. These results suggest that MBL contributes to the first-line host defense against SARS-CoV and that MBL deficiency is a susceptibility factor for acquisition of SARS

Serum levels of mannose-binding lectin in systemic sclerosis: a possible contribution to the initiation of skin sclerosis in the diffuse cutaneous subtype

2014 ◽  
Vol 24 (1) ◽  
pp. 123-125 ◽  
Author(s):  
Kaname Akamata ◽  
Yoshihide Asano ◽  
Naohiko Aozasa ◽  
Shinji Noda ◽  
Takashi Taniguchi ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Skin Sclerosis ◽  
Mannose Binding ◽  
Binding Lectin

Smoking status interacts with the association between mannose-binding lectin serum levels and gene polymorphism and the carriage of oropharyngeal bacteria

2010 ◽  
Vol 71 (3) ◽  
pp. 298-303 ◽  
Author(s):  
Ulla Jounio ◽  
Aino Rantala ◽  
Aini Bloigu ◽  
Raija Juvonen ◽  
Taina Lajunen ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Smoking Status ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin

Mannose Binding Lectin Acute Phase Activity in Patients with Severe Infection

2005 ◽  
Vol 25 (4) ◽  
pp. 346-352 ◽  
Author(s):  
M. M. Dean ◽  
R. M. Minchinton ◽  
S. Heatley ◽  
D. P. Eisen
Keyword(s):  
Acute Phase ◽  
Severe Infection ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Phase Activity

scholarly journals Low Serum Levels of Mannose Binding Lectin Are a Risk Factor for Neonatal Sepsis

2007 ◽  
Vol 61 (3) ◽  
pp. 325-328 ◽  
Author(s):  
Fabrizio de Benedetti ◽  
Cinzia Auriti ◽  
Leila E D'Urbano ◽  
Maria Paola Ronchetti ◽  
Lucilla Ravà ◽  
...  
Keyword(s):  
Risk Factor ◽  
Neonatal Sepsis ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Low Serum

scholarly journals Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study

2021 ◽  
Vol 35 ◽  
pp. 205873842110644
Author(s):  
Shereen A Baioumy ◽  
Shaimaa H Fouad ◽  
Shaimaa A Abdalgeleel ◽  
Ahmed A Baiomy ◽  
Dina E Sallam ◽  
...  
Keyword(s):  
Case Control Study ◽  
Serum Levels ◽  
Case Control ◽  
Recurrent Aphthous Stomatitis ◽  
Mannose Binding Lectin ◽  
Aphthous Stomatitis ◽  
Control Group ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Control Study

Objectives: Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case–control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Methods: Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. Results: The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545–1320) vs. 1760 ng/mL (1254–2134); p≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively ( p =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels ( p=0.685) or MBL2 codon 54 genotypes ( p=0.382) with the type of ulcers. Conclusion: There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.

scholarly journals Genetic Association With Pseudomonas aeruginosa Acquisition in Cystic Fibrosis: Influence of Surfactant Protein D and Mannose-Binding Lectin

2021 ◽  
Vol 12 ◽  
Author(s):  
Nasenien Nourkami-Tutdibi ◽  
Klemens Freitag ◽  
Michael Zemlin ◽  
Erol Tutdibi
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Genetic Variants ◽  
Serum Levels ◽  
Surfactant Protein ◽  
Mannose Binding Lectin ◽  
Surfactant Protein D ◽  
Risk Haplotype ◽  
Mannose Binding ◽  
Binding Lectin

Background:Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF) is associated with poor prognosis. Surfactant protein-D (SFTPD) and mannose-binding lectin (MBL) play a critical role in innate immunity and response to bacterial infections. We investigated serum levels and genetic variants of SFTPD and MBL in CF patients.Method: Thirty-five Caucasian patients homozygous for ΔF508del were genotyped for functional relevant polymorphisms within MBL2 (promoter−221 Y/X, codons 52, 54, and 57) and SFTPD genes (Met11Thr, Ala160Thr, and Ser270Thr). Serum levels of collectins, clinical characteristics, and PA status were correlated with genetic data.Results: Patients age, gender, and PA status did not affect MBL and SFTPD serum concentrations. MBL concentrations were correlated with MBL haplotypes. Patients with chronic Pseudomonas aeroginosa infection (PAC) and MBL insufficiency had a shorter interval between first PA infection and onset of PAC (0.01 vs. 4.6 years, p < 0.04) as well as a lower median age at transition to PAC (9.8 vs. 16.4 years, p < 0.03) compared to MBL sufficient patients with PAC. SFTPD serum level and FEV1% (Spearman r = −0.41, p < 0.03) showed a negative correlation irrespective of PA infection status. The hazard ratio to PA acquisition was increased in carriers of the SFTPD haplotype 11Thr-160Ala-270Ser compared to carriers of the common 11Met-160Thr-270Ser haplotype [HR 3.0 (95%CI: 1.1–8.6), p < 0.04].Conclusion: MBL insufficiency leads to a shorter interval between first PA infection and onset of chronic infection. Susceptibility to PA acquisition is associated with SFTPD genetic variants with 11Thr-160Ala-270Ser as risk haplotype for early PA infection. This may be due to presence of threonine associated with oligomeric structure of SFTPD and binding ability to bacteria.

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