Effects of different operating parameters on hydrogen production by Parageobacillus thermoglucosidasius DSM 6285

AbstractHydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen via the water–gas shift reaction catalyzed by a carbon monoxide dehydrogenase–hydrogenase enzyme complex. Here we have evaluated the effects of several operating parameters on hydrogen production, including different growth temperatures, pre-culture ages and inoculum sizes, as well as different pHs and concentrations of nickel and iron in the fermentation medium. All of the tested parameters were observed to have a substantive effect on both hydrogen yield and (specific) production rates. A final experiment incorporating the best scenario for each tested parameter showed a marked increase in the H2 production rate compared to each individual parameter. The optimised parameters serve as a strong basis for improved hydrogen production with a view of commercialisation of this process.

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Biohydrogen production by fermentive bacterium Clostridium sp. Tr2 using batch fermenter system controlled pH under dark fermentation

Journal of Vietnamese Environment ◽

10.13141/jve.vol9.no1.pp4-10 ◽

2018 ◽

Vol 9 (1) ◽

pp. 4-10

Author(s):

Thi Thu Huyen Nguyen ◽

Thi Yen Dang ◽

Thuy Hien Lai

Keyword(s):

Hydrogen Production ◽

Alternative Energy ◽

Fossil Fuels ◽

Dark Fermentation ◽

Gas Production ◽

Biohydrogen Production ◽

Great Promise ◽

Hydrogen Yield ◽

Promising Alternative ◽

Fermentative Bacteria

Limitation of fuels reserves and contribution of fossil fuels to the greenhouse effect leads to develop anew, clean and sustainable energy. Among the various options, biohydrogen appears as a promising alternative energy source. The fermentative hydrogen production process holds a great promise for commercial processes. Hydrogen production by fermentative bacteria is a very complex and greatly inﬂuenced by pH. This paper presents biohydrogen production by bacterial strain Clostridium sp. Tr2. Operational pH strongly affected its hyrogen production. Its gas production rate as well as obtained gas product were roughly increase twice under controlled pH at 6 than non-controlled condition. Dark fermentation for hydrogen production of strain Tr2 was performed under bottle as well as automatic fermenter scale under optimal nutritional and environmental conditions at 30°C, initial pH at 6.5, then pH was controlled at 6 for bioreactor scale (BioFlo 110). Bioreactor scale was much better for hydrogen production of strain Tr2. Clostridium sp. Tr2 produced 0.74 L hydro (L medium)-1 occupying 72.6 % of total gas under bottle scale while it produced 2.94 L hydro (L medium)-1 occupying 95.82 % of total gas under fermenter scale. Its maximum obtained hydrogen yield of Clostridium sp. Tr2 under bioreactor scale Bioflo 110 in optimal medium with controlled pH 6 was 2.31 mol hydro (mol glucose)-1. Dự trữ nhiên liệu có giới hạn và việc sử dụng nhiên liêu hoá thạch góp phần không nhỏ gây hiệu ứng nhà kính dẫn đến cần phải phát triển năng lượng mới, sạch và bền vững. Trong số các giải pháp, hydro sinh học xuất hiện như một nguồn năng lượng thay thế đầy hứa hẹn. Quá trình lên men sản xuất hydro có tiềm năng lớn để áp dụng trong sản xuất thương mại. Tuy nhiên qúa trình này rất phức tạp và chịu ảnh hưởng lớn bởi pH. Nghiên cứu này trình bày sản xuất hydro sinh học do chủng vi khuẩn Clostridium sp. Tr2. Quá trình sản xuất hydro của chủng này bị ảnh hưởng mạnh mẽ bởi pH thay đổi trong quá trình lên men. Tốc độ tạo khí cũng như lượng khí thu được của chủng này tăng gần gấp đôi trong môi trường có duy trì pH ở pH 6 so với môi trường không kiểm soát pH. Quá trình lên men tối sản xuất hydro của chủng Tr2 được thực hiện ở quy mô bình thí nghiệm cũng như bình lên men tự động trong điều kiện môi trường tối ưu ở 30°C, pH ban đầu 6.5, ở qui mô bình lên men tự động (BioFlo 110), pH môi trường sau đó được duy trì ổn định ở pH 6. Lên men sản xuất hdyro của chủng Tr2 trong bình lên men tự động tốt hơn rất nhiều so với lên men trong bình thí nghiệm. Clostridium sp. Tr2 chỉ tạo ra được 0,74 L hydro (L medium)-1 chiếm 72,6 % tổng thể tích khí thu được ở điều kiện lên men bình thí nghiệm trong khi chủng này sản xuất được 2,94 L hydro (L medium)-1 chiếm 95,82 % tổng thể tích khí ở điều kiện lên men tự động. Sản lượng hydro thu được lớn nhất của chủng này trong bình lên men tự động BioFlo 110 trong trong môi trường tối ưu có kiểm soát pH tại pH 6 là 2,31 mol hydro (mol glucose)-1.

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Cobalt-Based Metal-Organic Frameworks and Their Derivatives for Hydrogen Evolution Reaction

Frontiers in Chemistry ◽

10.3389/fchem.2020.592915 ◽

2020 ◽

Vol 8 ◽

Author(s):

Wenjuan Han ◽

Minhan Li ◽

Yuanyuan Ma ◽

Jianping Yang

Keyword(s):

Hydrogen Production ◽

Hydrogen Evolution ◽

Water Splitting ◽

Hydrogen Evolution Reaction ◽

Alternative Energy ◽

Metal Organic Frameworks ◽

Electrochemical Performances ◽

Promising Alternative ◽

Metal Organic ◽

Electrochemical Water Splitting

Hydrogen has been considered as a promising alternative energy to replace fossil fuels. Electrochemical water splitting, as a green and renewable method for hydrogen production, has been drawing more and more attention. In order to improve hydrogen production efficiency and lower energy consumption, efficient catalysts are required to drive the hydrogen evolution reaction (HER). Cobalt (Co)-based metal-organic frameworks (MOFs) are porous materials with tunable structure, adjustable pores and large specific surface areas, which has attracted great attention in the field of electrocatalysis. In this review, we focus on the recent progress of Co-based metal-organic frameworks and their derivatives, including their compositions, morphologies, architectures and electrochemical performances. The challenges and development prospects related to Co-based metal-organic frameworks as HER electrocatalysts are also discussed, which might provide some insight in electrochemical water splitting for future development.

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Hydrogen production from ethanol in nitrogen microwave plasma at atmospheric pressure

Open Chemistry ◽

10.1515/chem-2015-0039 ◽

2014 ◽

Vol 13 (1) ◽

Cited By ~ 4

Author(s):

Bartosz Hrycak ◽

Dariusz Czylkowski ◽

Robert Miotk ◽

Miroslaw Dors ◽

Mariusz Jasinski ◽

...

Keyword(s):

Hydrogen Production ◽

Atmospheric Pressure ◽

Alternative Energy ◽

Coal Gasification ◽

Microwave Plasma ◽

Atmospheric Pressure Plasma ◽

Water Electrolysis ◽

Experimental Tests ◽

Experimental Investigations ◽

Promising Alternative

AbstractHydrogen seems to be one of the most promising alternative energy sources. It is a renewable fuel as it could be produced from e.g. waste or bio-ethanol. Furthermore hydrogen is compatible with fuel cells and is environmentally clean. In contrast to conventional methods of hydrogen production such as water electrolysis or coal gasification we propose a method based on atmospheric pressure microwave plasma. In this paper we present results of the experimental investigations of hydrogen production from ethanol in the atmospheric pressure plasma generated in waveguide-supplied cylindrical type nozzleless microwave (2.45 GHz) plasma source (MPS). Nitrogen was used as a working gas. All experimental tests were performed with the nitrogen flow rate Q ranged from 1500 to 3900 NL h

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Production and Application of a Soluble Hydrogenase fromPyrococcus furiosus

Archaea ◽

10.1155/2015/912582 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Chang-Hao Wu ◽

Patrick M. McTernan ◽

Mary E. Walter ◽

Michael W. W. Adams

Keyword(s):

Hydrogen Production ◽

Alternative Energy ◽

Pyrococcus Furiosus ◽

Hydrogen Gas ◽

Energy Carrier ◽

Biotechnological Applications ◽

Biological Mechanisms ◽

Recombinant Enzymes ◽

Energy Needs

Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity’s growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophilePyrococcus furiosus, a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used inin vitrosystems for hydrogen production and NADPH generation and these systems are also discussed.

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PENGARUH UDARA TERHADAP FOTOLISIS AIR OLEH SERBUK TiO2 DENGAN ADANYA KALIUM IODIDA

Jurnal Riset Kimia ◽

10.25077/jrk.v3i2.28 ◽

2015 ◽

Vol 3 (2) ◽

pp. 112

Author(s):

Admin Alif ◽

Hermansyah Aziz ◽

Olly Norita Tetra ◽

Suci Mulya Prima

Keyword(s):

Potassium Iodide ◽

Alternative Energy ◽

Hydrogen Gas ◽

Mercury Lamp ◽

Maximum Volume ◽

Promising Alternative ◽

Alternative Energy Sources ◽

Sacrificial Agent ◽

Gas Volume ◽

Oxidation Agent

ABSTRACT Hydrogen is one of the promising alternative energy sources which is environmentally-friendly. Amongs several methods that have been used to produce hydrogen is a photolysis base on photochemical reaction which has been choosen and applied for this work. In this system where water was photolyzed in the present of TiO2 powder as photocatalyst and potassium iodide as sacrificial agent. The sample was prepared by addition various potassium iodide concentrations and then each sample was treated in the present and absent of air. The sample was placed in a quartz reactor which was then illuminated by a Philips low pressure vapoured mercury lamp (λ = 254 nm) as light source for 7 hours. Produced gas volume was measured by a method based on the movement of soap bubbles. The results showed that the maximum gas volume obtained at 0.006 g/L TiO2 and 0.1 g/L KI concentration (in the present and absent of air) were applied. Furthermore, the maximum volume of hydrogen gas was produced at the present of air. In the present of air, dissolved oxygen accelerated H2 gas productions which act as oxidation agent and electron carrier to H+. Keywords: Photolysis, Photocatalyst, Titania, Hydrogen, Sacrificial agent

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Special Issue on “Hydrogen Production Technologies”

Processes ◽

10.3390/pr8101268 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1268

Author(s):

Suttichai Assabumrungrat ◽

Suwimol Wongsakulphasatch ◽

Pattaraporn Lohsoontorn Kim ◽

Alírio E. Rodrigues

Keyword(s):

Power Generation ◽

Hydrogen Production ◽

Alternative Energy ◽

Energy Crisis ◽

Environmental Concerns ◽

Special Issue ◽

Promising Alternative ◽

Energy Carriers ◽

Production Technologies ◽

Chemical Products

According to energy crisis and environmental concerns, hydrogen has been driven to become one of the most promising alternative energy carriers for power generation and high valued chemical products [...]

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Hydrogen Production from Biomass and Organic Waste Using Dark Fermentation: An Analysis of Literature Data on the Effect of Operating Parameters on Process Performance

Processes ◽

10.3390/pr10010156 ◽

2022 ◽

Vol 10 (1) ◽

pp. 156

Author(s):

Rita Noelle Moussa ◽

Najah Moussa ◽

Davide Dionisi

Keyword(s):

Hydrogen Production ◽

Residence Time ◽

Organic Waste ◽

Dark Fermentation ◽

Low Ph ◽

Optimum Operating ◽

Operating Parameters ◽

Hydrogen Yield ◽

Process Performance ◽

Short Residence Time

In the context of hydrogen production from biomass or organic waste with dark fermentation, this study analysed 55 studies (339 experiments) in the literature looking for the effect of operating parameters on the process performance of dark fermentation. The effect of substrate concentration, pH, temperature, and residence time on hydrogen yield, productivity, and content in the biogas was analysed. In addition, a linear regression model was developed to also account for the effect of nature and pretreatment of the substrate, inhibition of methanogenesis, and continuous or batch operating mode. The analysis showed that the hydrogen yield was mainly affected by pH and residence time, with the highest yields obtained for low pH and short residence time. High hydrogen productivity was favoured by high feed concentration, short residence time, and low pH. More modest was the effect on the hydrogen content. The mean values of hydrogen yield, productivity, and content were, respectively, 6.49% COD COD−1, 135 mg L−1 d−1, 51% v/v, while 10% of the considered experiments obtained yield, productivity, and content of or higher than 15.55% COD COD−1, 305.16 mg L−1 d−1, 64% v/v. Overall, this study provides insight into how to select the optimum operating conditions to obtain the desired hydrogen production.

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Characteristics of hydrogen production from bean curd manufacturing waste by anaerobic microflora

Water Science & Technology ◽

10.2166/wst.2000.0401 ◽

2000 ◽

Vol 42 (3-4) ◽

pp. 345-350 ◽

Cited By ~ 51

Author(s):

O. Mizuno ◽

T. Ohara ◽

M. Shinya ◽

T. Noike

Keyword(s):

Hydrogen Production ◽

Organic Substrate ◽

Hydrogen Gas ◽

Hydrogen Yield ◽

Hydrogen Production Rate ◽

Fatty Acid Production ◽

Alcohol Production ◽

Carbohydrate Concentration ◽

Carbohydrate Degradation ◽

Anaerobic Microflora

The hydrogen production from bean curd manufacturing waste by anaerobic microflora was investigated using batch experiments at 35 °C. The anaerobic microflora was obtained from fermented soybean-meals and maintained using a sucrose-limited medium in continuous culture. A solution of an organic substrate without solid component such as rough fiber in bean curd manufacturing waste was used for the experiments. After the inoculation, hydrogen production immediately occurred and almost ceased at 12 h. The final concentration of hydrogen in gas produced was 63% H2. During hydrogen production, carbohydrate was rapidly degraded while protein degradation was hardly observed, suggesting that carbohydrate was the main source of the hydrogen production. The hydrogen yield was 2.54 mol of H2 mol-1 of hexose utilized if hydrogen gas was produced from only carbohydrate degradation. At a carbohydrate concentration greater than 3,720 mg l-1, the rate of hydrogen production rate significantly decreased. The rate of alcohol production was remarkably increased with increasing carbohydrate while the rate of volatile fatty acid production was hardly changed. The results indicated that the metabolic pathway and the amount of hydrogen production would be significantly influenced by the carbohydrate concentration.

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Hydrogen Production by Fluidized Bed Reactors: A Quantitative Perspective Using the Supervised Machine Learning Approach

J ◽

10.3390/j4030022 ◽

2021 ◽

Vol 4 (3) ◽

pp. 266-287

Author(s):

Zheng Lian ◽

Yixiao Wang ◽

Xiyue Zhang ◽

Abubakar Yusuf ◽

Lord Famiyeh ◽

...

Keyword(s):

Machine Learning ◽

Hydrogen Production ◽

Fluidized Bed ◽

Biomass Gasification ◽

Supervised Machine Learning ◽

Fluidized Bed Reactors ◽

Hydrogen Yield ◽

Carbon Conversion ◽

Operational Parameters ◽

Operational Conditions

The current hydrogen generation technologies, especially biomass gasification using fluidized bed reactors (FBRs), were rigorously reviewed. There are involute operational parameters in a fluidized bed gasifier that determine the anticipated outcomes for hydrogen production purposes. However, limited reviews are present that link these parametric conditions with the corresponding performances based on experimental data collection. Using the constructed artificial neural networks (ANNs) as the supervised machine learning algorithm for data training, the operational parameters from 52 literature reports were utilized to perform both the qualitative and quantitative assessments of the performance, such as the hydrogen yield (HY), hydrogen content (HC) and carbon conversion efficiency (CCE). Seven types of operational parameters, including the steam-to-biomass ratio (SBR), equivalent ratio (ER), temperature, particle size of the feedstock, residence time, lower heating value (LHV) and carbon content (CC), were closely investigated. Six binary parameters have been identified to be statistically significant to the performance parameters (hydrogen yield (HY)), hydrogen content (HC) and carbon conversion efficiency (CCE)) by analysis of variance (ANOVA). The optimal operational conditions derived from the machine leaning were recommended according to the needs of the outcomes. This review may provide helpful insights for researchers to comprehensively consider the operational conditions in order to achieve high hydrogen production using fluidized bed reactors during biomass gasification.

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Thermodynamic and Kinetic Considerations Regarding the Prospects for a Dual-Purpose Hydrogen Extraction and Separation Membrane

Energies ◽

10.3390/en14082136 ◽

2021 ◽

Vol 14 (8) ◽

pp. 2136

Author(s):

Karl Sohlberg

Keyword(s):

Hydrogen Production ◽

Hydrogen Abstraction ◽

Hydrogen Gas ◽

Catalytic Dehydrogenation ◽

Dual Purpose ◽

Physical Separation ◽

Extraction And Separation ◽

Separation Membrane ◽

Hydrocarbon Sources ◽

Critical Materials

Extraction of hydrogen from hydrocarbons is a logical intermediate-term solution for the escalating worldwide demand for hydrogen. This work explores the possibility of using a single membrane to accomplish both the catalytic dehydrogenation and physical separation of hydrogen gas as a possible way to improve the efficiency of hydrogen production from hydrocarbon sources. The present analysis shows that regions of pressure/temperature space exist for which the overall process is thermodynamically spontaneous (ΔG < 0). Each step in the process is based on known physics. The rate of hydrogen production is likely to be controlled by the barrier to hydrogen abstraction, with the density of H-binding sites also playing a role. A critical materials issue will be the strength of the oxide/metal interface.

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