scholarly journals An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Avinash Sharma ◽  
Rajvir Kaur ◽  
Jasleen Kaur ◽  
Saweta Garg ◽  
Rajbir Bhatti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Therapeutic Potential ◽  
Ethyl Acetate Extract ◽  
Schizophyllum Commune ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Fractional Excretion Of Sodium

AbstractThe present study aimed at isolation of endophytic basidiomycetous fungi and evaluation of their in-vitro and in-vivo antidiabetic potential. Preliminary screening for in-vitro activity was carried out using α-glucosidase inhibition assay. An endophytic isolate Sch1 (isolated from Aloe vera), identified to be Schizophyllum commune Fr. on molecular basis, exhibiting more than 90% α-glucosidase inhibitiory activity was selected for further studies. Detailed in-vivo investigations for antidiabetic potential of ethyl acetate extract of S. commune (Sch1), at two different doses, were carried out in streptozotocin induced diabetic Wistar rats. Treatment of diabetic rats with S. commune extract caused significant decrease in blood glucose level and increase in body weight after 14 days experimental period. It significantly restored renal parameters including creatinine, blood urea nitrogen, fractional excretion of sodium, and potassium level in diabetic rats. Improvement in lipid profile and level of antioxidant parameters viz. reduced glutathione, thiobarbituric acid reactive species, and superoxide anion generation was also observed after treatment. Liver enzymes (serum glutamic pyruvic transaminase, serum glutamic-oxaloacetic transaminases, and alkaline phosphatase) homeostasis was found to be markedly improved in diabetic rats administered with S. commune extract. The effects were more pronounced at higher concentration and comparable to acarbose which was used as positive control. Phytochemical analysis revealed the presence of phenolics and terpenoids in the ethyl acetate extract. This is the first report highlighting the therapeutic potential of an endophytic S. commune in the management of diabetes.

scholarly journals Persea americana Leaf Ethyl Acetate Extract Phytochemical, In-vitro Antioxidant and In-vivo Potentials to Mitigate Oxidative Stress in Alloxan-induced Hyperglycaemic Rats

2019 ◽  
pp. 1-11
Author(s):  
J. A. Mashi ◽  
A. M. Sa’id ◽  
R. I. Idris ◽  
I. Aminu ◽  
A. A. Muhammad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diabetic Rats ◽  
Persea Americana ◽  
Albino Rats ◽  
Standard Drug

The purpose of this study was to investigate the in-vivo and in-vitro potentials of ethyl acetate extract of P. americana leaf in alloxan-induced diabetic rats. Quantitative phytochemicals analyzed includes; flavonoids, saponins, tannins, alkaloids and phenolics. Measurement of antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl, total antioxidant capacity, hydroxyl radical, hydrogen peroxide, superoxide radical and ferric reducing activity of the extract was carried out. Hyperglycemia was induced by intraperitoneal injection of alloxan monohydrate to albino rats. In-vivo anti-oxidant potentials of the extract were evaluated by measuring liver homogenate activity of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and malondyaldehyde in alloxan-induced diabetic rats administered with the extract.  A total of 30 Albino rats were used for this experiment and they were divided into six groups of 5 rats each. Group A; normal control, Group B; diabetic control, Groups C-E; experimental groups administered with different doses (100, 200 and 400 mg/kg body weight respectively); of the extract and Group F; glucophage (84 mg/kg body weight, standard drug) for 4 weeks. This study was conducted in the Department of Biochemistry, Bayero University, Kano, in August, 2018. Data was analyzed using one-way ANOVA with P=.05 value considered as significant. Results of the quantitative phytochemical investigation shows that the extract is rich in phenolics (184.1±0.6), flavonoids (115.8±2.1), alkaloids (41.5±1.8), with least concentration of tannis (21.2±0.8) and saponins (15.2±2.3). The extract exhibited high radical scavenging activity against synthetic free radicals (DPPH), reactive oxygen species (peroxide, superoxide and hydroxyl acid) and high ability to reduce Fe3+ to Fe2+ (FRAP). The activities of antioxidant enzymes of the treated rats were increased significantly (P=.05) while the level malondyaldehyde was significantly decreased (P=.05) in the treated groups. Ethyl acetate leaf extract of Persea americana contains phytochemical substances which improved antioxidant status and can be use as herbal therapy for the management of oxidative stress induced by diabetes mellitus and associated complications.

scholarly journals ANTIDIABETIC POTENTIAL OF THE OYSTER MUSHROOM PLEUROTUS FLORIDA (MONT.) SINGER

2017 ◽  
Vol 9 (4) ◽  
pp. 51
Author(s):  
Prabu M. ◽  
Kumuthakalavalli R.
Keyword(s):  
Inhibitory Activity ◽  
Tamil Nadu ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Pleurotus Florida ◽  
Dose Dependent

Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida.Methods: The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously.Results: In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug.Conclusion: The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent.

scholarly journals Phytochemical Screening and Antidiabetic, Antihyperlipidemic, and Antioxidant Properties ofAnthyllis henoniana(Coss.) Flowers Extracts in an Alloxan-Induced Rats Model of Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Ameur Ben Younes ◽  
Maryem Ben Salem ◽  
Hanen El Abed ◽  
Raoudha Jarraya
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Histological Results

Background. This study investigates the biological activities ofAnthyllis henonianaflowers extracts.Materials and Methods. Antioxidant activity and thein vitroinhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. Diabetic rats were orally and daily given the best extract from flowers ofAnthyllis henonianaat a dose of acarbose for one month.Results. Among the extracts, the ethyl acetate one displayed remarkable antioxidant activity including DPPH (IC50= 2.34 mg/mL) and was more effective in inhibitingα-glucosidase (IC50= 17μg/mL) thanα-amylase (IC50= 920μg /mL) activities.In vivo, the results proved that ethyl acetate extract at doses of 400 mg/kg bw decreased significantly the blood glucose level and lipid profile levels and increased the activities of antioxidant enzymes. These protective impacts ofAnthyllis henonianaethyl acetate flowers extract were confirmed by histological results.Conclusion. This study demonstrates, for the first time, thatAnthyllis henonianaflowers ethyl acetate extract is effective in inhibiting hyperglycemia and oxidative stress caused by diabetes.

scholarly journals A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass

2020 ◽  
pp. 1-13
Author(s):  
K. Mercy Madhumitha ◽  
J. Anbumalarmathi ◽  
S. Aruna Sharmili ◽  
G. Nandhini ◽  
G. Shanmuga Priya
Keyword(s):  
Comparative Study ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition

The present study aims at comparative study between plant and callus extract, with respect to analysis of phytochemical constituents, antioxidant, antibacterial activity and cytotoxicity properties of Centratherum punctatum using aqueous medium and different solvents such as, methanol and ethyl acetate. In vitro studies in MS media supplemented with BAP 4.5 mg/L + Kn 4.0 mg/L has shown the high callus induction percentage of 92.33% with a maximum callus weight of 1.08 g. The phytochemical analysis of aqueous, methanol and ethyl acetate extract of C. punctatum in vivo plant and in vitro callus showed the presence of alkaloids, flavonoids, phenols and carbohydrates. The aqueous extract of both plant and callus showed the presence of tannins, proteins and steroids whereas the methanol extract showed the presence of tannins, amino acids and terpenoids. The ethyl acetate extract showed terpenoids and protein. FTIR analysis of plant and callus aqueous extract had a maximum characteristic band at 3399.87 cm-1 and 3412.73 cm-1 respectively indicating the presence of N-H stretching. GC-MS analysis revealed the presence of 11 different compounds in ethyl acetate extracts of plant and the callus extract revealed the presence of 15 different compounds which was absent in the plant extract. Plant extract exhibited maximum total phenol content than callus extract. The in vitro callus extract showed higher DPPH radical scavenging activity with lower inhibition percentage than in vivo plant extract. A maximum zone of inhibition was observed in methanol extract of in vivo plant and in vitro callus (15 mm and 14 mm respectively) against Bacillus subtilis. The ethyl acetate extract of in vivo plant and in vitro callus had a zone of 14 mm and 12 mm against E. coli. A maximum zone of inhibition (12 mm and 11 mm respectively) was observed in both methanol and ethyl acetate of in vivo plant and in vitro callus against Staphylococcus aureus. Antiproliferative analysis revealed that in vivo plant has inhibitory percentage of 23.6 whereas callus exhibited 28.5% against HeLa cells.

scholarly journals IN VITRO ANTIOXIDANT AND IN VIVO ANTIDIABETIC ACTIVITY OF TWO POTENTIAL PROBIOTIC ENTEROCOCCUS SPP. ON ALLOXAN-INDUCED DIABETIC RATS

2021 ◽  
pp. 94-98
Author(s):  
KAMNI RAJPUT ◽  
RAMESH CHANDRA DUBEY
Keyword(s):  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Animal Group ◽  
Enterococcus Spp

Objective: In vitro antioxidant activity, in vivo antidiabetic property and intestinal attachment by two potential probiotic bacterial strains, namely, Enterococcus faecium and Enterococcus hirae were studied using albino rats. Methods: Antioxidant the activity was assessed using 2,2-Diphenyl-1-picrylhydrazyl radicals scavenging assay. Alloxan was administered intraperitoneally to induce diabetic conditions in experimental rats. Animals were treated with oral administration of Enterococcus spp., such as E. faecium, and E. hirae isolated from goat and sheep milk. The control animal group received normal saline for the same days. Glibenclamide drug was used as a positive control against probiotic bacterial cells. Results: However, administration of probiotic bacterial strains E. faecium and E. hirae, in albino rats significantly (p<0.05) at varying doses lowered blood glucose levels in diabetic rats as compared to the diabetic control group. Both the species of Enterococcus increased the bodyweight of experimental rats. However, E. faecium was the best antidiabetic strain having the antioxidant activities also in comparison to E. hirae. The attachment of probiotic bacterial cells E. faecium on the rat’s intestine wall against pathogens was examined. Furthermore, E. faecium showed its aggregation with pathogens by attachment of the intestines of albino rats. This showed that both the bacterial strains exhibited in vivo antidiabetic effect. Conclusion: The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine.

In Vitro and In Vivo Anti-Inflammatory Activity of the Rhizomes of Globba pendula Roxb

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110559
Author(s):  
Le Minh Ha ◽  
Ngo Thi Phuong ◽  
Nguyen Thi Thu Hien ◽  
Pham Thi Tam ◽  
Do Thi Thao ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Inflammatory Activity ◽  
No Production ◽  
Anti Inflammatory ◽  
Inflammatory Effect

In this study, we aimed at evaluating in vitro and in vivo anti-inflammatory activity of various extracts of the rhizomes of Globba pendula Roxb. Three extracts ( n-hexane, ethyl acetate, and water) were screened for their inhibitory effect on NO production by lipopolysaccharide-stimulated RAW 264.7 macrophages. The ethyl acetate extract of G. pendula rhizomes (EGP) showed a potential effect with an IC50 value of 32.45 µg/mL. For in vivo study, the ethyl acetate extract was further investigated for its anti-inflammatory effect using collagen antibody-induced arthritic mice (CAIA). The level of arthritis in experimental mice significantly reduced ( P < .05) after treatment with EGP at a dose of 500 mg/kg body weight (b.w.). This study also revealed that EGP is orally non-toxic. Ethyl p-methoxy cinamate was identified as the main constituent of EGP, which may result in its anti-inflammatory effect.

scholarly journals INVESTIGATION OF PHARMACOGNOSTICAL, PHYTOCHEMICAL, AND PHARMACOLOGICAL ACTIVITY OF AERIAL ROOTS OF FICUS BENGHALENSIS LINN

2018 ◽  
Vol 11 (10) ◽  
pp. 279
Author(s):  
Kamalika Mazumder ◽  
Himangshu S Maji ◽  
Nripendra N Bala
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Analgesic Activity ◽  
Ethyl Acetate Extract ◽  
Microscopic Investigation ◽  
Tail Flick ◽  
Ficus Benghalensis ◽  
Aerial Roots

Objective: Ficus benghalensis Linn. (Moraceae family) is commonly known as banyan tree in English, which is used traditionally in India. The literature survey showed that the aerial roots of this plant are yet to be explored. Our main interest is to evaluate its pharmacognostic and phytochemical character by the standard monograph and to explore its in vitro antioxidant and in vivo analgesic activity study with ethyl acetate extract.Methods: Pharmacognostic evaluation and phytochemical screening have been done using standard monograph. An in vitro antioxidant activity using ethyl acetate extract has been done using four different methods. In vivo analgesic activity of the ethyl acetate extract has been evaluated by acetic acid-induced writhing test in mice and tail flick method.Results: Aerial roots of F. benghalensis have been found the rich source of steroidal glycosides, cardiac glycosides, flavonoids, tri-terpenoids, and phenols. The presence of phellem, phellogen, xylem, and phloem has been found after microscopic investigation. All the pharmacognostic parameters proved its purity. Results showed the absence of heavy metals. The ethyl acetate extract has shown potent antioxidant activity at 100 μg/ml concentration and higher analgesic activity at the concentration of 400 mg/kg than 200 mg/kg.Conclusion: Pharmacognostic characteristics and phytochemical properties revealed in this study could be used for the pharmacopoeial standard. Ethyl acetate extract showed potent antioxidant and analgesic activity.

scholarly journals Antimalarial Activity of Ethyl Acetate Extract and Fraction of Bidens pilosa against Plasmodium berghei (ANKA)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Noumedem Anangmo Christelle Nadia ◽  
Yamssi Cédric ◽  
Simeni Njonnou Sylvain Raoul ◽  
Ngongang Ouankou Christian ◽  
Mounvera Abdel Azizi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Control Group ◽  
Bidens Pilosa

Background. Malaria is one of the most critical diseases causing about 219 million cases worldwide in developing countries. The spread and development of resistance against chemical antimalarial drugs is one of the major problems associated with malaria control. The present study was to investigate the antimalarial efficacy of ethyl acetate extract and one fraction of Bidens pilosa in vivo in order to support the usage of this plant by traditional healers to treat malaria. Methods. The extracts were prepared by maceration of B. pilosa leaf powder in ethyl acetate. The liquid filtrate of the extract and the best in vitro antiplasmodial fraction using HPLC were concentrated and evaporated using a rotavapor under vacuum to dryness. The antimalarial activity of B. pilosa plant products were evaluated in vivo against Plasmodium berghei infected mice according to the Peter and Rane test. The antimalarial efficacy of the a selected crude extract (ethyl acetate extract) was evaluated at 125, 250, and 500 mg/kg, while a selected fraction from ethyl acetate extract (fraction 12) was evaluated at 62.5 and 125 mg/kg. Blood from experimental animals was collected to assess hematological parameters. Results. The crude extract of ethyl acetate and fraction 12 demonstrated 100% in vivo parasite suppressive activity at doses of 500 mg/kg and 125 mg/kg, respectively, for the crude extract and fraction 12. The mice treated with 250 and 500 mg/kg had their parasitemia (intraerythrocytic phase of P. Berghei) drop considerably, disappearing by the 8th day in mice receiving 500 mg/kg. The ethyl acetate extract of B. pilosa, fraction 12 showed an even higher antiplasmodial activity. By the 5th day of the experiment, the treatment led to a modification of hematological parameters in mice. The chloroquine (5 mg/kg), fraction 12 (125 mg/kg), and the crude extract (500 mg/kg) groups all survived the 30 days of the experiment, while the negative control group registered 100% of the deaths. Conclusion. This study scientifically supports the use of Bidens pilosa leaves in the traditional treatment of malaria. However, the mode of action and in vivo toxicity of the plant still need to be assessed.

scholarly journals In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

2017 ◽  
Vol 11 (1) ◽  
pp. 352-359 ◽  
Author(s):  
Gemechu Ameya ◽  
Aseer Manilal ◽  
Behailu Merdekios
Keyword(s):  
Nicotiana Tabacum ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Organic Solvents ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Modern Medicine ◽  
Phytochemical Analysis ◽  
Human Pathogens

Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria. Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents. Results: Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens. Conclusion: High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

scholarly journals Comparative extraction, phytochemical screening and in vitro biological activities of Eclipta prostrata extract

2020 ◽  
Vol 10 (4-s) ◽  
pp. 148-152
Author(s):  
Zafar Alam Khan ◽  
Salaj Khare ◽  
B.K. Dubey
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antidiabetic Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Eclipta Prostrata ◽  
Promising Source ◽  
Dose Dependent

Medicinal plants possess therapeutic potential and are used to treat various diseases around the world. Eclipta prostrate (L.) is a medicinal herb that has extensive application in the native medicinal system. In any therapeutic activity chemical constituents play an important role. Eclipta prostrata has been investigated in this study for its antioxidant, antimicrobial and antidiabetic activity in vitro. The well-known research protocol available in the literature established qualitative analysis of the different phytochemical constituents and quantitative analysis of total phenol and flavonoids. The hydroalcoholic extracts of the leaves and seeds of Eclipta prostrata exhibited significant and dose-dependent antioxidant activity including ability to donate electron. To analyze the antimicrobial activity, Leaves hydroalcoholic extracts and Eclipta prostrate seeds were tested against two selected strains using a well-diffusion method and showing significant inhibitory action against all the strain tested. In addition, the dose-dependent α-amylase inhibitory activity with an IC50 value of acarbose, leaves, and seed extract was found to be 364.89μg/ml and 438.43μg/ml, respectively, indicating that Eclipta prostrate is a promising source as an herbal medicine. Keywords: Eclipta prostrate, Phytochemical Analysis, Antioxidant, Antimicrobial, Antidiabetic Activity.

Sign in / Sign up

Export Citation Format

Share Document