scholarly journals Assessing the effects of accumulated Cd(II) on seed germination and root development of Arabidopsis thaliana

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Yejin Lee ◽  
Jinwoo Jang ◽  
Yangwon Jeon ◽  
Hyojin Kim ◽  
Geupil Jang ◽  
...  

AbstractIn this study, Arabidopsis thaliana was used as a model system to assess the toxic effects of cadmium on plant development and growth. The germination and growth of A. thaliana was inhibited by Cd(II), and the inhibitory effect was dosage-dependent. The significant decrease of germination rates and root growths of A. thaliana were observed from 50 mg/L and 25 mg/L of CdCl2, respectively. Although both shoot and root growths were suppressed by Cd(II), root developments were more sensitive to Cd(II) than shoot developments, as evidenced by shoot growths observed over 50 mg/L of CdCl2. In the concordance to this result, it was also observed that the expression of DR5::VENUS, a visual marker of auxin response, was dependent on the Cd(II) concentration and was strongly reduced from 5 mg/L of CdCl2. In addition, the E. coli-based biosensors were employed to quantify accumulated Cd(II) in plants to understand the correlation between toxic effects and Cd(II) in plants. As a result, it was revealed that 0.012 mg/g and 0.138 mg/g of Cd(II) in dried plants were corresponded to the concentration inhibiting root developments and root growths, respectively. Although it needs further investigations, the findings play a significant role in assessing the toxic effects of Cd(II) based on the relationship between the toxic effects and accumulated Cd(II) concentrations in plants.

1997 ◽  
Vol 60 (8) ◽  
pp. 943-947 ◽  
Author(s):  
PASCAL J. DELAQUIS ◽  
PETER L. SHOLBERG

A simple model system was constructed to evaluate the microbistatic and microbicidal properties of gaseous allyl isothiocyanate (AIT) against bacterial cells and fungal conidia deposited on agar surfaces. Salmonella typhimurium, Listeria monocytogenes Scott A, and Escherichia coli O157:H7 were inhibited when exposed to 1,000 μg AIT per liter. Pseudomonas corrugata, a Cytophaga species, and a fluorescent pseudomonad failed to grow in the presence of 500 μg AIT per liter. Germination and growth of Penicillium expansum, Aspergillus flavus, and Botrytis cinerea conidia was inhibited in the presence of 100 μg AIT per liter. Bactericidal and sporicidal activities varied with strain and increased with time of exposure, AIT concentration, and temperature. E. coli O157:H7 was the most resistant bacterial species tested.


2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Cristina Garcidueñas-Piña ◽  
Iliana E. Medina-Ramírez ◽  
Plinio Guzmán ◽  
Roberto Rico-Martínez ◽  
José Francisco Morales-Domínguez ◽  
...  

Nanostructured materials (NSMs) of silver (Ag@TiO2) and copper (TiO2-Cu2+) doped titanium dioxide were synthesized, fully characterized, and evaluated for their antimicrobial efficiency and effects onArabidopsis thaliana. The NSMs were prepared using an environmentally benign route. The physicochemical properties of the materials were determined with analytical techniques. These materials are active under visible light, exhibit a small size (10–12 nm), are crystalline (anatase), and liberate metal ions (Ag+and Cu2+) in solution. Microbicide activity was observed inE. coliC600 andS. cerevisiaeW303 strains treated with several concentrations of Ag@TiO2and TiO2-Cu2+, radiated and nonradiated, and after different times. Higher inactivation was achieved with Ag@TiO2inE. coli, with value of log inactivation of 2.2 with 0.5 mg/mL after 4 h, than inS. cerevisiae, with a log inactivation of 2.6 with 10 mg/mL after 24 h. The impact of these NSMs in plants was evaluated inArabidopsis thalianaCol-0 strain exposed to such materials at different conditions and concentrations, and physical and biochemical effects were analyzed. Seeds exposed to NSMs did not show effects on germination and growth. However, seedlings treated with these materials modified their growth and their total chlorophyll content.


2013 ◽  
Vol 295-298 ◽  
pp. 2333-2337
Author(s):  
Yun Gao Hu ◽  
Guo Tao Yang ◽  
Chong Hua Xie ◽  
Ling Zhang ◽  
Yong Jun Chen

Knowledge remains limited about whether the optimum culture condition of getting the most virulent M.oryzae toxins can be found and applied for resistant seed selection. The inhibition of seed germination and the growth of embryo and radicle in rice treated by the Magnaporthe grisea toxin that got from different culture conditions were studied. The result suggested that the M.oryzae toxin had great inhibitory effect on seed germination and growth of embryo and radicle in rice. The M.oryzae toxin cultivated on 23 °C, pH 8.0 for 20d has strongest inhibition on seed germination of the hybrid rice variety of GangYou725, the inhibition rate approach 70%; Being cultivated at the same pH and 23°C~28°C for approximate 10d, it is easy to get the M.oryzae toxin for high growth inhibition of embryo and radicle. It can reduce the workload and increase accuracy to use M.oryzae toxin to identify blast resistances of rice and select resistant materials.


2019 ◽  
Vol 25 (2) ◽  
Author(s):  
Subhash Chandra Yadav ◽  
Naveen Krishna Srivastava

Heavy metal pollution is a major environmental stress than can affect plant growth. The toxic effects of nickel on germination and growth of Coriander (Coriandrum sativum) seedling were investigated for 8 days. Growth of seedling parts especially radicle was greatly reduced for concentration at and above 50 mg/l


2021 ◽  
Vol 15 (4) ◽  
pp. 1563-1572
Author(s):  
Jacob Tchima Massai ◽  
Hamida Aminatou ◽  
Jean Boris Sounya ◽  
Dieudonné Ranava ◽  
Sebastien Vondou Vondou ◽  
...  

In Cameroon, despite the increased growing of cashew in recent years, orchard yields remain low due to the quality of seed and unsuitable peasant farming practices. This work realized in the nursery at Wakwa aimed at evaluating the effect of different concentrations of salt on cashew germination and growth. The trial was conducted during the rainy season. The substrate was made up of a mixture of sand, black soil and cow manure respectively at 1/4, 1/2 and 1/4. Seeds were soaked in different proportions of salt solution (5%, 10% and 15%) for 24 hours. The experimental design was a complete randomized block comprising four treatments, each of which was replicated three times. Treatments consisted of different percentage of salt (5%. 10% and 15%) and the control without salt (0%). Salt concentration acted in different ways on germination, survey rate and plants growth. Germination inhibition by salt changed according to the salt concentration and time, being highest at the start of experiment and decrease over time. At 28DAS, 5% and 10% concentrations did not have an inhibitory effect, which made it possible to improve survey rate at this time. Conversely, 15% had a stimulating effect on the vigor and plants growth of cashew. This study showed that soaking cashew seeds in the salt after 24 hours at different concentration does not delay germination and concentration 15% allow to obtained well growth and more vigorous plants.


Author(s):  
Snežana Andjelkovič ◽  
Snežana Babić ◽  
Jasmina Milenković ◽  
Vladimir Zornić ◽  
Miladen Prijović ◽  
...  

This study presents the results of testing the effect of different concentrations of lead-acetate on seed germination and young Seedlings of alfalfa. Тhe experiment was organized so that in the first variant, only lead-acetate of different concentrations (10-5, 10-4, 10-3, 10-2 and 2 x 10-2 M) was used, and in the second, next to the lead acetate EDTA concentration of 0.012 % was added. The results of this study in which natural conditions are imitated showed that the percentage of alfalfa seed germination decreases with increasing lead-acetate concentrations. In a medium of high concentration of lead acetate 10-2 and 2 x 10-2, a small number of seeds (39% and 32 (32%) germinated. In the same treatments with EDTA, the toxic effects of lead acetate were reduced, and seed germination was better (59% and 43% seeds germinated). It has been noticed that lead has toxic effects on the growth of alfalfa roots and stems. In the variant in which EDTA was used, the negative influence of lead on and growth of alfalfa seedlings was significantly mitigated.


Author(s):  
Snežana Andjelkovič ◽  
Snežana Babić ◽  
Jasmina Milenković ◽  
Vladimir Zornić ◽  
Miladen Prijović ◽  
...  

This study presents the results of testing the effect of different concentrations of lead-acetate on seed germination and young Seedlings of alfalfa. Тhe experiment was organized so that in the first variant, only lead-acetate of different concentrations (10-5, 10-4, 10-3, 10-2 and 2 x 10-2 M) was used, and in the second, next to the lead acetate EDTA concentration of 0.012 % was added. The results of this study in which natural conditions are imitated showed that the percentage of alfalfa seed germination decreases with increasing lead-acetate concentrations. In a medium of high concentration of lead acetate 10-2 and 2 x 10-2, a small number of seeds (39% and 32 (32%) germinated. In the same treatments with EDTA, the toxic effects of lead acetate were reduced, and seed germination was better (59% and 43% seeds germinated). It has been noticed that lead has toxic effects on the growth of alfalfa roots and stems. In the variant in which EDTA was used, the negative influence of lead on and growth of alfalfa seedlings was significantly mitigated.


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