scholarly journals The effect of 4-hexylresorinol administration on NAD+ level and SIRT activity in Saos-2 cells

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
In-Song Lee ◽  
Jun-Ho Chang ◽  
Dae-Won Kim ◽  
Seong-Gon Kim ◽  
Tae-Woo Kim
Keyword(s):  
Untreated Control ◽  
Expression Levels ◽  
Anti Oxidant

Abstract Background 4-hexylresorcinol (4HR) has been shown to have anti-oxidant activity similar to that of resveratrol. As resveratrol increases sirtuin (SIRT) activity, 4HR might behave similarly to resveratrol. Method In this study, the expression levels of SIRT1, SIRT3, and SIRT6 were evaluated after 4HR administration (1–100 μM). As NAD+ is a substrate for SIRTs, its levels with SIRT activity were also studied. Results In the results, SIRT3 (100 μM at 24 h) and SIRT6 (1–100 μM at 24 h and 10 μM at 8 h) were shown to have significantly higher expression levels compared to untreated control (p < 0.05). Pan-SIRT activity and the NAD+ level was significantly increased compared to that of the untreated control (p < 0.05; 10 and 100 μM at 24 h). Conclusion 4HR administration increased SIRT activity and the NAD+ level in Saos-2 cells.

scholarly journals Anti-hyperalgesic properties of a flavanone derivative Poncirin in acute and chronic inflammatory pain models in mice

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ruqayya Afridi ◽  
Ashraf Ullah Khan ◽  
Sidra Khalid ◽  
Bushra Shal ◽  
Hina Rasheed ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Inflammatory Pain ◽  
Expression Levels ◽  
Chronic Inflammatory Pain ◽  
Qrt Pcr ◽  
Pain Models ◽  
Anti Oxidant ◽  
Mrna Expression Levels

Abstract Background Poncirin is flavanone derivative (isolated from Poncirus trifoliata) with known pharmacological activities such as anti-tumor, anti-osteoporotic, anti-inflammatory and anti-colitic. The present study aimed to explore the anti-allodynic and anti-hyperalgesic potentials of poncirin in murine models of inflammatory pain. Methods The analgesic potential of poncirin was evaluated in formalin-, acetic acid-, carrageenan- and Complete Freund’s Adjuvant (CFA)-induced inflammatory pain models in mice. Anti-allodynic and anti-hyperalgesic activities were measured using Von Frey filaments, Randall Selitto, hotplate and cold acetone tests. The serum nitrite levels were determined using Griess reagent. The Quantitative Real-time PCR (qRT-PCR) was performed to assess the effect of poncirin on mRNA expression levels of inflammatory cytokines and anti-oxidant enzymes. Results Intraperitoneal administration of poncirin (30 mg/kg) markedly reduced the pain behavior in both acetic acid-induced visceral pain and formalin-induced tonic pain models used as preliminary screening tools. The poncirin (30 mg/kg) treatment considerably inhibited the mechanical hyperalgesia and allodynia as well as thermal hyperalgesia and cold allodynia. The qRT-PCR analysis showed noticeable inhibition of pro-inflammatory cytokines (mRNA expression levels of TNF-α, IL-1β and IL-6) (p < 0.05) in poncirin treated group. Similarly, poncirin treatment also enhanced the mRNA expressions levels of anti-oxidant enzymes such as transcription factor such as nuclear factor (erythroid-derived 2)-like 2 (Nrf2) (p < 0.05), heme oxygenase (HO-1) (p < 0.05) and superoxide dismutase (SOD2) (p < 0.05). Chronic treatment of poncirin for 6 days did not confer any significant hepatic and renal toxicity. Furthermore, poncirin treatment did not altered the motor coordination and muscle strength in CFA-induced chronic inflammatory pain model. Conclusion The present study demonstrated that poncirin treatment significantly reduced pain behaviors in all experimental models of inflammatory pain, suggesting the promising analgesic potential of poncirin in inflammatory pain conditions.

Restoring Effects of Natural Anti-Oxidant Quercetin on Cellular Senescent Human Dermal Fibroblasts

2018 ◽  
Vol 46 (04) ◽  
pp. 853-873 ◽  
Author(s):  
Eun-Ju Sohn ◽  
Jung Min Kim ◽  
Se-Hui Kang ◽  
Joseph Kwon ◽  
Hyun Joo An ◽  
...  
Keyword(s):  
Target Genes ◽  
Functional Decline ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Gene Expressions ◽  
Expression Levels ◽  
Anti Oxidant ◽  
Flavonoid Quercetin ◽  
Quercetin Treatment

The oxidative damage initiated by reactive oxygen species (ROS) is a major contributor to the functional decline and disability that characterizes aging. The anti-oxidant flavonoid, quercetin, is a plant polyphenol that may be beneficial for retarding the aging process. We examined the restoring properties of quercetin on human dermal fibroblasts (HDFs). Quercetin directly reduced either intracellular or extracellular ROS levels in aged HDFs. To find the aging-related target genes by quercetin, microarray analysis was performed and two up-regulated genes LPL and KCNE2 were identified. Silencing LPL increased the expression levels of senescence proteins such as p16INK4A and p53 and silencing KCNE2 reversed gene expressions of EGR1 and p-ERK in quercetin-treated aged HDFs. Silencing of LPL and KCNE2 decreased the expression levels of anti-oxidant enzymes such as superoxide dismutase and catalase. Also, the mitochondrial dysfunction in aged HDFs was ameliorated by quercetin treatment. Taken together, these results suggest that quercetin has restoring effect on the cellular senescence by down-regulation of senescence activities and up-regulation of the gene expressions of anti-oxidant enzymes in aged HDFs.

scholarly journals Genistein Enhances the Promoting Effects of Exercise on Anti-Oxidant and Anti- Inflammation Balance and Cardiomyopathy in the Ovariectomized Diabetic Rat

2021 ◽  
Author(s):  
Farzaneh Nazari-Serenjeh ◽  
Seyyed Alireza Vafaie ◽  
Maryam Khajvand-Abedini ◽  
Amir Mohammad Salehi ◽  
Mohammad Mohammadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cardiac Tissue ◽  
Ovariectomized Rats ◽  
Diabetic Rat ◽  
Cardiac Complications ◽  
Expression Levels ◽  
Stress Markers ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Combined Intervention

Abstract Purpose: These experiments aimed to analyze the effects of genistein and/or exercise on the expression of miRNA-133, IGF-1, and Bcl-2, Bax plus oxidative stress and inflammation markers in the heart of diabetic ovariectomized rats. Methods: Animals were divided into seven groups (n=8): control, sham, ovariectomy (OVX), diabetic ovariectomized (OD), and diabetic ovariectomized with eight weeks of genistein administration (ODG) and with eight weeks of swimming training (ODS) and with eight weeks of both of them (ODGS). High-fat nutrition and low dose streptozotocin injection were used for induction of diabetes. The effect of those treatments was evaluated by measuring lipid profiles, miRNA-133 gene expression, and Bcl-2, Bax, and IGF-1 protein expression levels. Inflammation and oxidative stress markers levels were also measured.Results: ovariectomy down-regulated cardiac miRNA-133, IGF-1, and Bcl-2 expression levels and increased MDA, OSI, TOS, TNF- α, and NF-κB levels plus a reduction in TAC. Diabetes induced an additive effect on the above-measured factors. Genistein improved oxidative and inflammation conditions and up-regulated miRNA-133, Bcl-2, and IGF-1 expression in OD rats. Genistein also enhanced the positive effect of exercise on miRNA-133, Bcl-2, and IGF-1expression in the heart, along with a reduction in Bax. The combined intervention was associated with improvement in oxidative and inflammation conditions. Histological observation showed some abnormality in cardiac tissue, which was improved by genistein or/and exercise treatment.Conclusion: genistein or/and exercise as a natural replacement therapy could improve diabetic-induced cardiac complications within the heart of ovariectomized rats.

Exogenous γ-tocotrienol promotes preimplantation development and improves the quality of porcine embryos

2015 ◽  
Vol 27 (3) ◽  
pp. 481 ◽  
Author(s):  
Enok Lee ◽  
Sung-Hun Min ◽  
Bong-Seok Song ◽  
Ji-Yeong Yeon ◽  
Jin-Woo Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treated Group ◽  
Apoptotic Index ◽  
Developmental Competence ◽  
Blastocyst Development ◽  
Expression Levels ◽  
Preimplantation Stage ◽  
Anti Oxidant ◽  
Bax Gene

γ-tocotrienol (GTT), an isomer of vitamin E, has been the subject of increasing interest due to its strong anti-oxidant effects. Therefore, in this study, the effects of GTT on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in preimplantation porcine embryos. After in vitro maturation and fertilisation, porcine embryos were cultured for 6 days in porcine zygote medium 3 supplemented with or without GTT (200 μM) under oxidative stress conditions (200 μM hydrogen peroxide (H2O2)). Blastocyst development was significantly improved in the GTT-treated group when compared with the H2O2-treated group (P < 0.05). Subsequent evaluation of the intracellular levels of ROS and numbers of apoptotic nuclei in GTT-treated blastocysts revealed that ROS levels of GTT-treated porcine blastocysts were decreased (P < 0.05) and the numbers of apoptotic nuclei were reduced by GTT treatment in porcine embryos. Moreover, the total cell numbers of blastocysts were significantly increased in the GTT-treated group relative to the untreated group under H2O2-induced oxidative stress (P < 0.05). The expression levels of apoptosis-related genes (BCL-XL, BAX) in GTT-treated blastocysts were then investigated using real-time reverse transcription polymerase chain reaction. Expression of the anti-apoptotic BCL-XL gene was shown to be increased in the GTT-treated blastocyst group, whereas expression of the pro-apoptotic BAX gene was decreased. Taken together, these results suggest that GTT (200 μM) under H2O2-induced oxidative stress, thereby improving the developmental competence of porcine embryos via modulation of intracellular levels of ROS and the apoptotic index during the preimplantation stage.

Ciliary coordination in newt lung cells requires microtubule-based linkages between basal bodies: A correlative light and EM study

1992 ◽  
Vol 50 (1) ◽  
pp. 570-571
Author(s):  
Robert Hard ◽  
Gerald Rupp ◽  
Matthew L. Withiam-Leitch ◽  
Lisa Cardamone
Keyword(s):  
Basal Body ◽  
Untreated Control ◽  
Beat Frequency ◽  
Primary Cultures ◽  
Living Cells ◽  
Power Stroke ◽  
Ultrastructural Changes ◽  
Lung Cells ◽  
Basal Bodies ◽  
Ciliated Cells

In a coordinated field of beating cilia, the direction of the power stroke is correlated with the orientation of basal body appendages, called basal feet. In newt lung ciliated cells, adjacent basal feet are interconnected by cold-stable microtubules (basal MTs). In the present study, we investigate the hypothesis that these basal MTs stabilize ciliary distribution and alignment. To accomplish this, newt lung primary cultures were treated with the microtubule disrupting agent, Colcemid. In newt lung cultures, cilia normally disperse in a characteristic fashion as the mucociliary epithelium migrates from the tissue explant. Four arbitrary, but progressive stages of dispersion were defined and used to monitor this redistribution process. Ciliaiy beat frequency, coordination, and dispersion were assessed for 91 hrs in untreated (control) and treated cultures. When compared to controls, cilia dispersed more rapidly and ciliary coordination decreased markedly in cultures treated with Colcemid (2 mM). Correlative LM/EM was used to assess whether these effects of Colcemid were coupled to ultrastructural changes. Living cells were defined as having coordinated or uncoordinated cilia and then were processed for transmission EM.

Potential anti-diabetic and anti-oxidant activity of essential oil of Zataria multiflora leaves

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YY Kamrani ◽  
M Amanlou ◽  
A Yazdanyar ◽  
A AdliMoghaddam ◽  
SN Ebrahimi
Keyword(s):  
Essential Oil ◽  
Zataria Multiflora ◽  
Anti Oxidant

Gene expression analysis of metabolic markers during bone regeneration in a rat model

2014 ◽  
Vol 23 (03) ◽  
pp. 207-211
Author(s):  
C. Kasch ◽  
A. Osterberg ◽  
Thordis Granitzka ◽  
T. Lindner ◽  
M. Haenle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Saline Solution ◽  
Female Rats ◽  
Synthesis Rate ◽  
Metabolic Markers ◽  
Expression Levels ◽  
Fibrotic Tissue ◽  
Intermittent Pth ◽  
Lower Expression

SummaryThe RANK/RANKL/OPG system plays an important role in the regulation of bone metabolism and bony integration around implants. The aim of this study was to analyse gene expression of OPG, RANK, and RANKL in regenerating bone during implant integration. Additionally, the effect of intermittent para - thyroid hormone (PTH) treatment was analysed. A titanium chamber was implanted in the proximal tibiae of 48 female rats. The animals received either human PTH or saline solution (NaCl). After 21 and 42 days, RNA was isolated from tissue adjacent to the implant and expression of RANK, RANKL, and OPG was analysed. After 21 days, very low expression levels of all genes were shown. In contrast, increased gene expression after 42 days was determined. Expression of RANK and RANKL was lower than that for OPG. The lower expression levels after 21 days might be due to still ossifying, fibrotic tissue around the titanium chamber. An increased OPG synthesis rate associated with decreased RANKL expression after 42 days revealed bone-forming processes. Despite significant differences in gene expression between the time points, only slight differences were observed between application of intermittent PTH and NaCl after a period of 42 days.

Sign in / Sign up

Export Citation Format

Share Document