Synergetic effects of Docetaxel and ionizing radiation reduced cell viability on MCF-7 breast cancer cell

Controlled production of cyclin dependent kinases (CDK) and stabilization of tumor suppressor genes are the most important factors involved in preventing carcinogenesis. The present study aimed to explore the cyclin dependent apoptotic effect of nymphayol on breast cancer MCF-7 cells. In our previous study, we isolated the crystal from a chloroform extract of Nymphaea stellata flower petals and it was confirmed as nymphayol (17-(hexan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol) using x-ray diffraction (XRD), Fourier transform infrared (FTIR), and mass spectroscopy (MS) methods. The cytotoxic effect of nymphayol on MCF-7 cells were analyzed using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The cellular and nuclear damage was determined using propidium iodide (PI) and acridine orange/ethidium bromide (AO/ErBr) staining. Tumor suppressor and apoptosis related mRNA transcript levels were determined using real-time polymerase chain reaction (RT-PCR). Nymphayol potentially inhibits MCF-7 cell viability up to 78%, and the IC50 value was observed as 2.8 µM in 24 h and 1.4 µM in 48 h. Treatment with nymphayol significantly increased reactive oxygen species (ROS) level and the tunnel assay confirmed DNA damage. We found characteristically 76% apoptotic cells and 9% necrotic cells in PI and AO/ErBr staining after 48 h treatment with 2.8 µM of nymphayol. Gene expression analysis confirmed significantly (p ≤ 0.001) increased mRNA levels of cyclin dependent kinase inhibitor 2A (Cdkn2a), retinoblastoma protein 2 (pRb2), p53, nuclear factor erythroid 2-factor 2 (Nrf2), caspase-3, and decreased B-cell lymphoma 2 (Bcl-2), murine double minute 2 (mdm2), and proliferating cell nuclear antigen (PCNA) expression after 48 h. Nymphayol effectively inhibited breast cancer cell viability, and is associated with early expression of Cdkn2a, pRb2, and activation of p53 and caspases.

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Assessing of integration of ionizing radiation with Radachlorin-PDT on MCF-7 breast cancer cell treatment

Lasers in Medical Science ◽

10.1007/s10103-015-1844-0 ◽

2015 ◽

Vol 31 (2) ◽

pp. 213-219 ◽

Cited By ~ 12

Author(s):

R. Ghoodarzi ◽

V. Changizi ◽

A. R. Montazerabadi ◽

N. Eyvazzadaeh

Keyword(s):

Breast Cancer ◽

Ionizing Radiation ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Mcf 7

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PIPERINE IN THE PREVENTION OF THE DECREASED TAMOXIFEN SENSITIVITY IN MCF-7 BREAST CANCER CELL LINE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.74 ◽

2018 ◽

Vol 10 (1) ◽

pp. 335

Author(s):

Sandy Vitria Kurniawan ◽

Lies Sugiarti ◽

Septelia Inawati Wanandi ◽

Melva Louisa

Keyword(s):

Breast Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cells ◽

Cancer Cell Line ◽

P Glycoprotein ◽

Mcf 7 ◽

In Cells

Objective: This study was designed to analyze the role of piperine in modulating P-glycoprotein mRNA expression when added in combination withtamoxifen to breast cancer cells in culture.Methods: MCF-7 breast cancer cells were treated with 1 μM tamoxifen with or without piperine (12.5, 25, or 50 μM) or verapamil 50 μM (P-glycoproteininhibitor positive control) for up to 12 days. We assessed the cell viability and isolated total RNA from them. We quantified P-glycoprotein expressionsusing quantitative reverse transcription polymerase chain reaction.Results: Administration of various doses of piperine decreased MCF-7 breast cancer cell viability. Piperine, when given in combination with tamoxifen,decreased the expression of P-glycoprotein mRNA in cells compared with the expression in cells treated with tamoxifen only. The effects were shownto be dose dependent.Conclusion: Piperine prevents the development of breast cancer cell tamoxifen resistance, probably through its inhibition of P-glycoprotein expression.

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Research on the Enhancement Mechanism of Dihydromyricetin on the Inhibitory Role of Cisplatin Towards Breast Cancer Cell Activity

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2022.2980 ◽

2022 ◽

Vol 12 (5) ◽

pp. 989-995

Author(s):

Ke Chunlin ◽

Dong Feng ◽

Wang Peirong

Keyword(s):

Breast Cancer ◽

Wound Healing ◽

Cell Viability ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cell Activity ◽

Cell Number ◽

Enhancement Mechanism ◽

Mcf 7

Objective: The purpose of our study was to evaluate Enhancement Mechanism of Dihydromyricetin (DMY) on the Inhibitory Role of Cisplatin Towards Breast Cancer Cell Activity. Materials and Methods: The MCF-7 were divided into NC, DMY, Cis and DMY+Cis groups. Using relative methods (MTT, TUNEL, Transwell, flow cytometry and wound healing) to evaluate MCF-7 cell biological activities including cell viability, apoptosis, invasion cell number and wound healing rate. The relative proteins expressions including FOXO-1, Noxa, Bim, Cyto C, Caspase-3, Caspase-9 and Apaf-1 were evaluated by WB assay. Results: MCF-7 cell viability, invasion cell number and wound healing rates were significantly depressed and apoptosis rate were significantly increased in DMY, Cis and DMY+Cis groups (P < 0.01, respectively). Compared with Cis group, cell viability, invasion cell number and wound healing rates were significantly depressed and apoptosis rate were significantly increased in DMY+Cis group (P < 0.05, respectively). Conclusion: Dihydromyricetin can effectively enhance the inhibitory effect of cisplatin on breast cancer cells.

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Comparison of the effects of nobiletin and letrozole on the activity and expression of aromatase in the MCF-7 breast cancer cell line

Biochemistry and Cell Biology ◽

10.1139/bcb-2016-0206 ◽

2017 ◽

Vol 95 (4) ◽

pp. 468-473 ◽

Cited By ~ 4

Author(s):

Seyedeh Tayebeh Rahideh ◽

Mohammad Keramatipour ◽

Mitra Nourbakhsh ◽

Fariba Koohdani ◽

Mostafa Hoseini ◽

...

Keyword(s):

Breast Cancer ◽

Cell Viability ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cell Line ◽

Cancer Cell Line ◽

Dependent Manner ◽

Mtt Assays ◽

Mcf 7

Nobiletin (NOB) is one of the polymethoxyflavones mainly found in citrus fruits. Aromatase or cytochrome P450 (CYP19) enzyme catalyzes the last and rate-limiting step in estrogen biosynthesis. This study was carried out to investigate the effect of NOB on the activity and expression of aromatase, and to compare this property with letrozole (LET) as aromatase inhibitor in the MCF-7 breast cancer cell line. Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays. Aromatase enzyme activity based on the conversion of androgenic substrate testosterone into 17β-estradiol was determined. CYP19 gene expression was measured by quantitative real-time PCR. MTT assays demonstrated that NOB at a concentration of 100 μmol/L decreased cell viability in a time-dependent manner (P < 0.05). NOB significantly inhibited aromatase at the concentration of 0.1 μmol/L (P = 0.013), whereas other concentrations had no effect. Treatment with 10 μmol/L and 1 μmol/L of NOB for 48 h significantly increased (P = 0.001) and decreased (P = 0.02) relative aromatase expression, respectively. The combination of LET and NOB had no effect on aromatase. This study showed for the first time that NOB decreases the activity and expression of aromatase at low concentrations in MCF-7 breast cancer cells.

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Okra-derived dietary Carotenoid lutein against Breast Cancer, with an Approach towards Developing a Nutraceutical Product: A Meta-analysis Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i40a32230 ◽

2021 ◽

pp. 135-142

Author(s):

Abd Elmoneim O. Elkhalifa ◽

Eyad Al-Shammari ◽

Mohammad Jahoor Alam ◽

Jerold C. Alcantara ◽

Mushtaq Ahmad Khan ◽

...

Keyword(s):

Breast Cancer ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Meta Analysis ◽

Analysis Data ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Mcf 7

Objective: Cancer chemoprevention with phytochemicals such as “lutein” derived from the vegetable okra could prove beneficial. Therefore, the objective of this study was to perform a meta-analysis of “lutein” against the breast cancer cell lines (MCF-7) and to establish the possible development of lutein based nutraceuticals. Methodology: A literature survey was performed using online data bases such as PubMed, Google scholar, and EMBASE, from 2000 to 2020 by using keywords such as “Lutein”, “Anticancer activity”, “Breast cancer cell lines”, and “MCF-7”. Studies reported lutein anticancer potentials against MCF-7 were included in the study. Results: Out of 28 studies, 7 research articles fulfilled the inclusion criteria. Meta-analysis data indicated that, a lutein concentration at ≥1 µM was able to reduce the MCF-7 cell viability of 59.837 with a 95% confidence interval (CI): 48.331 to 71.343. Additionally, a forest plot of the cumulative studies also indicated that impact of lutein concentration to reduce the MCF-7 cell viability was around 60%. Moreover, the I2 value of lutein was 74%, which is a considerable heterogeneity. Conclusion: Therefore, based upon the meta-analysis data, the conclusion is that dietary lutein supplementation and fortification of food with clinical data could be an approach to develop a nutraceutical product for preventive, as well as for adjunct therapeutic purposes in various breast cancer subtypes.

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ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF ERUCA SATIVA (JARJEER) LEAVES EXTRACT ON CARCINOMA OF WOMEN’S BREAST

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i2.39337 ◽

2021 ◽

pp. 89-92

Author(s):

SAFAA A DERBALA ◽

MANAR E ELKADY ◽

REHAB A ELBANHAWY ◽

ABDEL-AZIZ AF

Keyword(s):

Breast Cancer ◽

Antioxidant Enzymes ◽

Cell Viability ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cell Line ◽

Cancer Cell Line ◽

Eruca Sativa ◽

Mcf 7

Objective: This work aims to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7). Methods: In vitro, breast cancer cell line (MCF-7) treated by E. sativa leaves extract for 48 h. The cell viability, proliferation, and apoptosis were assessed using colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric technique, and antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) measurement. Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significative reduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induces a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2 and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD and CAT activities were significantly increased in the administered cells compared with unadministered MCF-7 cells. Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7. This may help in protection from breast cancer or preclinical recommendation.

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