Serum estrogen and its soluble receptor levels in Egyptian patients with acute leukemia: case-control study

Abstract Background Acute leukemias are malignant neoplastic diseases that arise from either lymphoid [ALL] or myeloid [AML] cell lines that are distinguished by the proliferation of BM non-functional immature cells and subsequently released into the bloodstream. ALL is prevalent malignancy in young, while AML in older. Diagnosis is usually routinely performed through peripheral blood count and smear then confirmed by BM aspirate. It is remarkable to notice that leukemia can be manifested at high, low, and even at normal leucocyte count. While treatment results have improved steadily over the last decades in younger and adults, limited changes have been in survival among subjects of age > 60 years. Aim of the work is to measure the serum estrogen [E2] and its soluble receptor [ER] levels in acute leukemia patients and extrapolate its possible clinical significance. This study included 40 [20 females and 20 males] healthy volunteers clinically free from any disease, 40 [20 females and 20 males] AML patients, and 40 [20 females and 20 males] ALL. To all subjects, serum E2 and its soluble ER level were investigated by ELISA. Results Serum E2 [pg/ml] level was lower in AML and ALL female and male patients groups than control group. Serum ER [ng/ml] level was lower in AML and ALL female and male patients groups than control group. Conclusion Estimation of serum E2 and its soluble ER level is of edifying diagnostic value. Determination of serum E2 and its soluble ER level in AML and ALL patients is of value in deciding treatment therapeutic target protocol.

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Possibilities of Application of Modern Neurophysiological Methods in Diagnosis of Vibration Disease

Acta biomedica scientifica ◽

10.29413/abs.2018-3.6.11 ◽

2019 ◽

Vol 3 (6) ◽

pp. 82-87

Author(s):

L. S. Vasileva ◽

N. V. Slivnitsyna ◽

O. L. Lakhman ◽

O. I. Shevchenko

Keyword(s):

Thermal Sensitivity ◽

Statistical Processing ◽

Diagnostic Value ◽

Pathological Process ◽

Cold Sensitivity ◽

Control Group ◽

Vibration Disease ◽

Equilibrium Function ◽

Constant Potential

Vibration disease is one of the leading in the structure of occupational diseases. A more detailed study of the pathogenesis, expanding the possibilities of diagnosis of vibration disease, is necessary. The aim of the study is to determine the diagnostic value of stabilometry, quantitative sensory testing and neuroenergocarting in vibration disease associated with the combined effects of local and general vibration. Materials and methods. Two groups of patients were examined: with the diagnosis of vibration disease associated with the combined effect of local and general vibration – 50 people (age 48.7 ± 3.1 years), not in contact with vibration – 30 people (age 49.1 ± 2.8 years). The study of the equilibrium function, the determination of the level of the constant potential of the brain by 12 standard leads and the determination of temperature and pain sensitivity. Statistical processing of the results was carried out using the software package “Statistica 6.0” (StatSoft Inc., USA). Differences were considered statistically significant at p < 0.05. Results. In vibration disease, the vertical stance of patients is more unstable than in patients with no contact with vibration. In patients with vibration disease, there is a decrease in the threshold of cold sensitivity and an increase in the threshold of thermal sensitivity and thermal pain compared to the control group. According to our data, the increased level of permanent potential in patients with vibration disease is observed in the central (22.7 (12.6; 30.7) mV) and right central (20.4 (11.5; 27.1) mV), also the average level of permanent capacity in this group is increased – 17.1 (8.4; 25.8) mV.Conclusion. The results obtained indicate the involvement of the neurosensory complex united by single thalamic and cortical centers in the pathological process and confirm the diagnostic value of the methods used.

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Urinary excretion of type IV collagen as an early marker of renal fibrosis in patients with diabetes mellitus

Diabetes Mellitus ◽

10.14341/2072-0351-5813 ◽

2011 ◽

Vol 14 (4) ◽

pp. 29-31 ◽

Cited By ~ 2

Author(s):

Irina Arkad'evna Bondar' ◽

Vadim Valer'evich Klimontov ◽

Ekaterina Mikhailovna Parfent'eva ◽

Vyacheslav Vital'evich Romanov ◽

Alexander Petrovich Nadeev

Keyword(s):

Type 1 Diabetes ◽

Urinary Excretion ◽

Renal Fibrosis ◽

Type Iv Collagen ◽

Diagnostic Value ◽

Control Group ◽

Type Iv ◽

Patients With Diabetes

Aim. To determine the diagnostic value of urinary excretion of type IV collagen in patients with type 1 diabetes with different stages of nephropathy.Methods. Urinary type IV collagen was determined in 60 patients with type 1 diabetes (23 with normal albuminuria, 28 with microalbuminuriaand 9 with macroalbuminuria) by an enzyme immunoassay. 10 healthy individuals were acted as the control group. Renal biopsy was performedin 22 patients. Deposits of type IV collagen were revealed by 11 individuals by immunohistochemistry. Results. The urinary excretion of type IV collagen increased with severety of diabetic nephropathy, correlating with the urinary albumin/creatinineratio, serum creatinine and parameters of daytime and nighttime systolic and diastolic blood pressure. Patients with excessive accumulation of typeIV collagen in the glomeruli had significantly higher level of type IV collagen in the urine. Conclusion. The determination of urinary type IV collagen can be used for early detection of renal fibrosis in patients with type 1 diabetes.

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Diagnostic value of anti-CD74 antibodies in early and late axial spondyloarthritis and its relationship to disease activity

Rheumatology ◽

10.1093/rheumatology/keaa292 ◽

2020 ◽

Vol 60 (1) ◽

pp. 263-268 ◽

Cited By ~ 2

Author(s):

Marwa Mahmoud Abdelaziz ◽

Rania M Gamal ◽

Nadia M Ismail ◽

Raghda A Lafy ◽

Helal F Hetta

Keyword(s):

Disease Activity ◽

Axial Spondyloarthritis ◽

Diagnostic Value ◽

Inactive Disease ◽

Control Group ◽

Igg Antibodies ◽

Control Groups ◽

Significant Difference ◽

Healthy Control

Abstract Objectives This study was designed to evaluate the role of anti-CD74 antibodies in diagnosis of axial spondyloarthritis (axSpA) and their relationship to disease duration and disease activity. Methods Fifty patients with axSpA, 15 patients with RA and 15 healthy subjects were included in the study. Clinical examination and laboratory tests were done. The ESR, CRP level and ASDAS were measured as markers of the disease activity. Quantitative determination of human CD74 IgG antibodies was done. Results The mean age of the patients was 38.22 (S.D.12.20) years. The level of CD74 autoantibodies was significantly higher in axSpA in comparison to control groups. Most patients with positive articular and extra-articular manifestations were positive for CD74 autoantibodies. In patients with inactive disease, 33.3% were positive for CD74 autoantibodies, as were 83% with active disease. High percentages of patients with early and late axSPA were CD74 autoantibody positive. The majority of patients with positive disease activity in early and late axSpA were CD74 autoantibody positive. CD74 autoantibodies had 80% sensitivity vs both control groups with 87% specificity vs the healthy control group and 80% vs the RA control group in the diagnosis of axSpA. Conclusions The frequency of positive anti-CD74 IgG antibodies was as high in patients with early axSpA as in those with late axSpA, with no significant differences. There was a significant difference in the frequency of positive anti-CD74 IgG antibodies between patients with positive and negative disease activity. Based on the sensitivity and specificity of anti-CD74 IgG, this is a promising diagnostic tool to support the clinical diagnosis of axSpA.

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AML1-ETO Collaborates with the Homeobox Gene Meis1 in Inducing Acute Leukemia in the Mouse Bone Marrow Transplantation Model.

Blood ◽

10.1182/blood.v112.11.928.928 ◽

2008 ◽

Vol 112 (11) ◽

pp. 928-928 ◽

Cited By ~ 3

Author(s):

Vegi M. Naidu ◽

Vijay P.S. Rawat ◽

Christina Schessl ◽

Konstantin Petropoulus ◽

Monica Cusan ◽

...

Keyword(s):

Bone Marrow ◽

Acute Leukemia ◽

Insertional Mutagenesis ◽

Fusion Gene ◽

Genetic Alterations ◽

Control Group ◽

Mouse Bone Marrow ◽

Acute Leukemias ◽

Hox Gene ◽

Blast Cells

Abstract AML1-ETO is the most frequent fusion gene in human AML. Previously, we and others have demonstrated that the fusion is not able to cause leukaemia on its own in experimental murine models, but that it needs collaborative partners. However, although mutations such as the FLT3-length mutation and C-KIT mutations were defined as important collaborative genetic events in AML1-ETO positive AML, most human AML1-ETO cases do not carry these mutations, indicating the presence of unkown collaborative partners in these patients. On the other hand Meis1, a HOX gene co-factor, belonging to the TALE family of homeodomain proteins, has a well established function as a protooncogene with a strong collaborative potential in Hox gene associated AML in mice. First we confirmed expression of MEIS1 in some patients with AML1-ETO positive AML by real-time PCR. Based on this we sought to determine if AML1-ETO can collaborate with Meis1 in inducing acute leukemias: single constructs or both genes were co-transfected in 5-FU treated primary murine bone marrow cells by retroviral gene transfer, using MSCV retroviral constructs with an IRES–GFP or YFP cassette. Mice were transplanted with BM cells expressing Meis1 alone (n=10), with BM cells solely expressing the fusion gene (n=10) or EGFP (n=7, control) or with BM expressing both genetic alterations (n=14). None of the mice in the Meis1 and AML1-ETO as well as in the control group developed disease. In contrast, 14 mice transplanted with BM co-expressing AML1-ETO and Meis1 developed lethal disease after a median latency of 102 days. Three mice succumbed to a myeloproliferative syndrome and nine mice died by acute leukemia (6 mice developed AML, 3 mice ALL), which was serially transplantable into secondary recipients (median = 57 days). Immunohistochemistry of various organs of leukemic mice showed massive infiltration with blast cells. In MPS and AML 85 ± 9.3 % of the blast cells co-expressed Gr-1+ and Mac1+. In ALL cases 40 ± 19.9 % of the malignant cells co-expressed Mac1 and the lymphoid-associated B220 antigen. Analysis of retroviral integration did not reveal recurrent integration sites as an indication for insertional mutagenesis. In summary, our data demonstrate for the first time that AML1-ETO can collaborate with Meis1 and identify a novel collaborative partner in t(8;21) positive AML. Furthermore, our analyses demonstrate that Meis1 can collaborate with non-homeobox genes in inducing acute leukemia.

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Determining Acute Leukemia Lineage Using Mie Map Red Blood Cell

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v28i1.1747 ◽

2021 ◽

Vol 28 (1) ◽

pp. 1

Author(s):

Nelly Zuroidah ◽

Arifoel Hajat ◽

Paulus Budiono Notopuro

Keyword(s):

Acute Leukemia ◽

Sensitivity And Specificity ◽

Bone Marrow Aspiration ◽

High Specificity ◽

Peripheral Blood Smear ◽

Diagnostic Value ◽

Lymphoid Leukemia ◽

Lymphoid Lineage ◽

The Matrix

The determination of myeloid and lymphoid lineage is essential for the diagnosis and therapy of acute leukemia. Immunophenotyping is the gold standard to determine the lineage of acute leukemia, but it is still constrained and relatively expensive. Mie Map RBC in the ADVIA 2120i is a parameter that can give additional information about myeloid and lymphoid lineage but has never been studied before. It is expected that Mie Map RBC can be used to differentiate the lineage of acute myeloid and lymphoid leukemia if immunophenotyping is not present. This study aimed to analyze the diagnostic value of Mie Map RBC with ADVIA 2120i towards immunophenotyping in determining myeloid and lymphoid lineage in acute leukemia. Child and adult patients diagnosed with acute leukemia (n=30) that had peripheral blood smear and bone marrow aspiration with blasts > 20% were examined using ADVIA 2120i. The Mie Map RBC lineage results were compared to the lineage of immunophenotyping. The sensitivity and specificity of the Mie Map RBC myeloid series are respectively 60.00%, 93.33%. The sensitivity and specificity of the Mie Map RBC lymphoid series are respectively 93.33% and 60.00%. The diagnostic accuracy value of Mie Map RBC is 76.67%. The determination of acute leukemia myeloid series lineage has high specificity. If there is no population outside the matrix of Mie Map RBC, it highly suggests myeloid series. On the other hand, the determination of acute leukemia lymphoid series lineage has a relatively low specificity meaning that the population outside the matrix of Mie Map RBC does not always suggest a lymphoid lineage

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CLINICAL AND DIAGNOSTIC VALUE OF DETERMINATION OF NEOPTERIN IN ATOPIC DERMATITIS IN CHILDREN

Kuban Scientific Medical Bulletin ◽

10.25207/1608-6228-2018-25-3-24-27 ◽

2018 ◽

Vol 25 (3) ◽

pp. 24-27

Author(s):

M. M. BEN ◽

E. YU. YANCHEVSKAYA ◽

O. A. MESNYANKINA

Keyword(s):

Atopic Dermatitis ◽

Skin Diseases ◽

Secondary Infection ◽

Clinical Manifestations ◽

Diagnostic Value ◽

Laboratory Method ◽

Control Group ◽

Allergic Skin ◽

Monitoring And Forecasting

Aim. To improve the diagnosis of allergic skin diseases in childrenMaterials and methods. 135 children aged from 2 to 6 with atopic dermatitis were examined during exacerbation. They were on inpatient treatment in the skin and venereologic department of the Regional skin and venereologic dispensary and the Department of Allergology of the Regional children's clinical hospital. At the same time, 103 people (the main group) had uncomplicated course of the disease, 32 patients had complications of atopic dermatitis in the form of secondary infection. The control group consisted of 29 children aged 2 to 6. Neopterin in serum was determined by enzyme-linked immunosorbent analysis using test kits "Neopterin ELISA" (Germany). Statistical reliability of the obtained data was evaluated using The Student's test.Results. Atopic dermatitis in children, regardless of clinical manifestations, is accompanied by an increase in the content of neopterin in serum. The nature of the increase in the level of neopterin is determined by the duration of the disease, while its highest values are characteristic of the prolonged course of the process (over 4 years).Conclusion. The determination of neopterin in children with atopic dermatitis is a promising laboratory method for assessing the severity of dermatosis, monitoring and forecasting the further course of the process, as well as evaluating the effectiveness of therapeutic measures.

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The Diagnostic Value of N-Acetyl-β-D-Glucosaminidase and Microalbumin Concentrations in Rheumatoid Arthritis

Journal of Medical Biochemistry ◽

10.2478/v10011-007-0038-0 ◽

2007 ◽

Vol 26 (4) ◽

pp. 300-308 ◽

Cited By ~ 1

Author(s):

Dejan Spasovski ◽

Todor Gruev ◽

Nada Marina ◽

Jordan Calovski ◽

Snežana Percinkova ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Colorimetric Assay ◽

Diagnostic Value ◽

Control Group ◽

Rf Test ◽

Diagnostic Values ◽

Healthy Control ◽

Laboratory Variables ◽

Higher Sensitivity

The Diagnostic Value of N-Acetyl-β-D-Glucosaminidase and Microalbumin Concentrations in Rheumatoid ArthritisThe purpose of this research was to compare the diagnostic values of laboratory variables, to present quantitative evaluations of the diagnostic sifted test with reference to sensitivity and specificity, the predictive value of the positive and negative test and precision of the test for N-acetyl-β-D-glucosa-minidase (NAG), microalbumin, rheumatoid factor (RF), Creactive protein (CRP), DAS 28 index, in early diagnosis of untreated rheumatoid arthritis (RA), and to define the effect of untreated rheumatoid arthritis on glomerular and tubular function. Using a colorimetric assay for the determination of Nacetyl-β-D-glucosaminidase and an immunoturbidimetric assay for the determination of urinary albumin, the samples of serum and urine have been examined in 70 participants (35 RA who were not treated, 35 healthy controls). RF was defined with the test for agglutination (Latex RF test) in the same participants. Out of 35 examined patients with RA, in 13 we found the presence of NAG enzymuria (sensitivity of the test 37.14%), while microalbuminuria appeared in 4 patients (sensitivity of the test 11.42%). RF appeared in 17 patients (sensitivity of the test 48.57%). Four patients were NAG and RF positive, while 3 patients were microalbuminuria and RF positive. Among 18 RF negative patients, 9 patients were NAG positive, and 1 patient presented with microalbuminuria. Among 17 RF positive RA, the presence of NAG was found in 4 patients, and the presence of microalbuminuria in 3 patients. Among 18 RF negative RA, NAG enzymuria appeared in 9 patients. Microalbuminuria was present in 1 patient. In the healthy control group, 8 patients were NAG positive, 2 patients were positive for microalbuminuria. RF appeared in 2 patients. NAG has higher sensitivity than microalbuminuria in the detection of asymptomatic renal lesions in untreated RA.

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Mitochondrial Dysfunction Contributes to Aging-Related Atrial Fibrillation

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/5530293 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Chuanbin Liu ◽

Jing Bai ◽

Qing Dan ◽

Xue Yang ◽

Kun Lin ◽

...

Keyword(s):

Atrial Fibrillation ◽

Mitochondrial Dysfunction ◽

Telomere Length ◽

Medical Center ◽

Diagnostic Value ◽

Control Group ◽

Elderly Male ◽

Blood Leukocytes ◽

Male Patients ◽

And Gender

The incidence of atrial fibrillation (AF) increases with age, and telomere length gradually shortens with age. However, whether telomere length is related to AF is still inconclusive, and the exact mechanism by which aging causes the increased incidence of AF is still unclear. We hypothesize that telomere length is correlated with aging-related AF and that mitochondrial dysfunction plays a role in this. This research recruited 96 elderly male patients with AF who were admitted to the Second Medical Center of Chinese PLA General Hospital from April to October 2018. After matching by age and gender, 96 non-AF elderly male patients who were admitted to the hospital for physical examination during the same period were selected as controls. Anthropometric, clinical, and laboratory analyses were performed on all subjects. The mitochondrial membrane potential (MMP) of peripheral blood leukocytes was detected as the indicator of mitochondrial function. Compared with the control group, the leukocyte telomere length (LTL) was significantly shorter ( P < 0.001 ), and the level of PGC-1α in serum was significantly lower in AF patients. Additionally, in subjects without any other diseases, the AF patients had lower MMP when compared with the control. Multivariate logistic regression confirmed that LTL (OR 0.365; 95% CI 0.235-0.568; P < 0.001 ) and serum PGC-1α (OR 0.993; 95% CI 0.988-0.997; P = 0.002 ) were inversely associated with the presence of AF. In addition, ROC analysis indicated the potential diagnostic value of LTL and serum PGC-1α with AUC values of 0.734 and 0.633, respectively. This research concludes that LTL and serum PGC-1α are inversely correlated with the occurrence of aging-related AF and that mitochondrial dysfunction plays a role in this.

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Evaluation of ELectrolytes in Adult Patients with Acute Leukemia before and after Chemotherapy

Baghdad Science Journal ◽

10.21123/bsj.10.2.362-367 ◽

2013 ◽

Vol 10 (2) ◽

pp. 362-367

Author(s):

Baghdad Science Journal

Keyword(s):

Acute Leukemia ◽

White Blood Cells ◽

Essential Element ◽

Serum Levels ◽

The Body ◽

Control Group ◽

Serum Electrolytes ◽

Hormonal Function ◽

Before And After

Abstract:Leukemia is a cancer of early blood forming cells. Most of them are cancers of white blood cells , however some leukemias start in other blood cell types.Electrolytes have modulatory effects on several biological mechanisms in the body namely as stabilizers,element of structures, essential element for hormonal function and also co-factors for a number of enzymes.In this study serum electrolytes levels were measured in patients with acute leukemia (AL) disorders before and after chemotherapy(anthracycline, doxorubicin, cytarabine ,prednisone, vincristine and doxorubicin) during one month and compared with that of control group. Blood samples were obtained from (43) patients (28 males and 15 females) aged (15-55)years;juset before and after chemotherapy. The control group contained samples from (40) healthy volunteers (26 males and 14 females) aged (15-55) years.Serum electrolytes levels(sodium Na+1,potassium K+1,calcium Ca+1,chloride Cl-1,magnesium Mg+2and phosphate PO4-3) were estimated using flame atomic absorbtion photometry. Serum levels of Na, K,Ca and Cl were significantly decreased in patients before chemotherapy in comparasion with that of control group. The mean concentration of serum phosphoruse and magnesium in acute leukemia patients was non significant compared with that of control group. In this study, determination of serum electrolytes in leukemic patients indicates an abnormal metabolic process in these patients

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Neopteryna jako wskaźnik stanu zapalnego i rozrostu nowotworowego w ostrych białaczkach

Medycyna Rodzinna ◽

10.25121/mr.2018.21.1.27 ◽

2018 ◽

Vol 21 (1) ◽

Author(s):

Jadwiga Nowicka ◽

Wiesława Nahaczewska ◽

Iwona Urbanowicz ◽

Mieczysław Woźniak

Keyword(s):

Acute Leukemia ◽

Viral Infections ◽

Lymphoblastic Leukemia ◽

Elevated Serum ◽

Control Group ◽

Inflammatory Condition ◽

Acute Leukemias ◽

Serum Neopterin ◽

Neopterin Concentration ◽

Cellular Immune

Introduction. Neopterin (NPT) is a sensitive marker for cellular immune responses. It is a pteridine group compound as a dye substance in insects, lower vertebrata and mammals. Neopterin is released from human monocytes, macrophages and dendritic cells upon stimulation by interferon gamma produced by T-lymphocytes. High neopterin concentrations in serum and urine were shown to be a reliable indicator for the severity of bacterial, viral infections including autoimmune diseases, allograft rejections and various malignant disorders. Aim. The aim of the study was the concentration of the neopterin in acute leukemias may be an endogenous marker of unfavorable processes in acute leukemia for which the growth of the tumor, the coexistence of inflammation. Material and methods. The studies involved 80 patients suffering from acute leukemias including 53 patients with acute myeloid leukemia, 21 patients with acute lymphoblastic leukemia and 6 patients with mixed phenotype acute leukemia. The patients with acute leukemia was analyzed as a group with inflammatory condition and a group without inflammatory condition. The quantitative assessment of serum neopterin level was performed by means of immunoenzymatic test ELISA. Results. Patients with all types of leukemia showed elevated serum neopterin levels in comparison to the control group and significantly elevated neopterin levels in patients with coexisting inflammation compared to the values of these parameters in patients without inflammation. The neopterin concentration was highest in the group of patients diagnosed with acute M4 and M5 leukemia, both without inflammation (32.8 ± 13.6 nmol/l) and with co-existing inflammation (116.57 ± 97.0 nmol/l) (p = 0.00024). Conclusions. Neopterin as a marker of malignant hyperplasia may be used only in cases where inflammation does not occur.

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