Determining Acute Leukemia Lineage Using Mie Map Red Blood Cell

2021 ◽  
Vol 28 (1) ◽  
pp. 1
Author(s):  
Nelly Zuroidah ◽  
Arifoel Hajat ◽  
Paulus Budiono Notopuro
Keyword(s):  
Acute Leukemia ◽  
Sensitivity And Specificity ◽  
Bone Marrow Aspiration ◽  
High Specificity ◽  
Peripheral Blood Smear ◽  
Diagnostic Value ◽  
Lymphoid Leukemia ◽  
Lymphoid Lineage ◽  
The Matrix

The determination of myeloid and lymphoid lineage is essential for the diagnosis and therapy of acute leukemia. Immunophenotyping is the gold standard to determine the lineage of acute leukemia, but it is still constrained and relatively expensive. Mie Map RBC in the ADVIA 2120i is a parameter that can give additional information about myeloid and lymphoid lineage but has never been studied before. It is expected that Mie Map RBC can be used to differentiate the lineage of acute myeloid and lymphoid leukemia if immunophenotyping is not present. This study aimed to analyze the diagnostic value of Mie Map RBC with ADVIA 2120i towards immunophenotyping in determining myeloid and lymphoid lineage in acute leukemia. Child and adult patients diagnosed with acute leukemia (n=30) that had peripheral blood smear and bone marrow aspiration with blasts > 20% were examined using ADVIA 2120i. The Mie Map RBC lineage results were compared to the lineage of immunophenotyping. The sensitivity and specificity of the Mie Map RBC myeloid series are respectively 60.00%, 93.33%. The sensitivity and specificity of the Mie Map RBC lymphoid series are respectively 93.33% and 60.00%. The diagnostic accuracy value of Mie Map RBC is 76.67%. The determination of acute leukemia myeloid series lineage has high specificity. If there is no population outside the matrix of Mie Map RBC, it highly suggests myeloid series. On the other hand, the determination of acute leukemia lymphoid series lineage has a relatively low specificity meaning that the population outside the matrix of Mie Map RBC does not always suggest a lymphoid lineage

scholarly journals Diagnostic Value of Determination Blast Cell Population Lineage Using WPC Scattergram Hematology Analyzer

2020 ◽  
Vol 26 (3) ◽  
Author(s):  
Nina Ratnasari ◽  
Arifoel Hajat ◽  
S. Ugroseno Yudho Bintoro
Keyword(s):  
Cell Population ◽  
Hematological Malignancies ◽  
Hematological Malignancy ◽  
Bone Marrow Aspiration ◽  
Diagnostic Value ◽  
Population Based ◽  
Blast Cell ◽  
Precursor Cell ◽  
Lymphoid Lineage

The diagnosis of hematology malignancies requires examination that includes morphology, immunophenotyping, and cytogenetics. Immunophenotyping is the most trusted examination in determining hematology malignancies lineage, but it is only available in large hospitals and the costs are relatively expensive, so the determination of lineage depends on bone marrow aspiration examination. Therefore it is necessary to have an easier and more reliable alternative to assist BMA morphology. White Precursor Cell (WPC) scattergram Sysmex XN-1000 has the capability to differentiate malignancy lineage. The purpose of this study was to determine the diagnostic value of determining lineage generated by WPC scattergram compared to the lineage from BMA examination. BMA blood samples were simultaneously examined by BMA morphology interpretation using microscope and WPC scattergram Sysmex XN-1000 examination. The hematology malignancies lineage resulting from BMA and WPC scattergram examination was then analyzed statistically to determine the suitability, sensitivity, and specificity. The results of determining the lineage of blast cell population based on WPC scattergram resulted in a suitability with a sensitivity of 93.75% and specificity of 94.74% for determining the hematological malignancy of myeloid lineage and 94.74% and 93.75% for lymphoid lineage, with a diagnostic accuracy of 94.91%. Based on this study it can be concluded that the WPC scattergram can determine the lineage of hematological malignancies with a suitability and high diagnostic value of lineage based on BMA morphology.

Determination of tissue polypeptide-specific antigen in pleural fluid and serum from patients with pleural effusion

1995 ◽  
Vol 10 (3) ◽  
pp. 161-165 ◽  
Author(s):  
V. Villena ◽  
J. Echave-Sustaeta ◽  
A. Lopez-Encuentra ◽  
P. Martin-Escribano ◽  
J. Estenoz-Alfaro ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Pleural Fluid ◽  
Sensitivity And Specificity ◽  
Specific Antigen ◽  
Diagnostic Value ◽  
Pleural Effusions ◽  
Malignant Pleural Effusions ◽  
Cytologic Analysis

As a tool for differentiating malignant and benign pleural effusions, we evaluated the diagnostic value of the assay of tissue polypeptide-specific antigen (TPS) in pleural fluid and serum, and of the pleural fluid TPS/serum TPS ratio in patients with pleural effusion. We studied prospectively 147 consecutive patients who had pleural effusions: 43 malignant pleural effusions and 104 benign pleural effusions. TPS levels were measured by RIA. The sensitivity and specificity of these measurements were: TPS in pleural fluid (cutoff 20,000 U/L): 0.21 and 0.98; TPS in serum (cutoff 300 U/L): 0.31 and 0.96; pleural fluid TPSI serum TPS ratio (cutoff 1200): 0.07 and 0.99. All these values enhanced the sensitivity of cytologic analysis of pleural fluid. However, we conclude that TPS assay in pleural fluid and serum, and the pleural fluid TPSI serum TPS ratio have limited diagnostic value in patients with pleural effusion.

scholarly journals Serum estrogen and its soluble receptor levels in Egyptian patients with acute leukemia: case-control study

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Samir Ali Abd El-Kaream ◽  
Samia Abd El-Moneim Ebied ◽  
Nadia Ali Sadek ◽  
Dina Mohamed Saad ◽  
Eman Attia Nadwan
Keyword(s):  
Acute Leukemia ◽  
Diagnostic Value ◽  
Control Group ◽  
Soluble Receptor ◽  
Acute Leukemias ◽  
Egyptian Patients ◽  
Male Patients ◽  
Value Determination ◽  
Immature Cells

Abstract Background Acute leukemias are malignant neoplastic diseases that arise from either lymphoid [ALL] or myeloid [AML] cell lines that are distinguished by the proliferation of BM non-functional immature cells and subsequently released into the bloodstream. ALL is prevalent malignancy in young, while AML in older. Diagnosis is usually routinely performed through peripheral blood count and smear then confirmed by BM aspirate. It is remarkable to notice that leukemia can be manifested at high, low, and even at normal leucocyte count. While treatment results have improved steadily over the last decades in younger and adults, limited changes have been in survival among subjects of age > 60 years. Aim of the work is to measure the serum estrogen [E2] and its soluble receptor [ER] levels in acute leukemia patients and extrapolate its possible clinical significance. This study included 40 [20 females and 20 males] healthy volunteers clinically free from any disease, 40 [20 females and 20 males] AML patients, and 40 [20 females and 20 males] ALL. To all subjects, serum E2 and its soluble ER level were investigated by ELISA. Results Serum E2 [pg/ml] level was lower in AML and ALL female and male patients groups than control group. Serum ER [ng/ml] level was lower in AML and ALL female and male patients groups than control group. Conclusion Estimation of serum E2 and its soluble ER level is of edifying diagnostic value. Determination of serum E2 and its soluble ER level in AML and ALL patients is of value in deciding treatment therapeutic target protocol.

scholarly journals FAMACHA©: Predictive value for control of Haemonchus sp. in sheep from Brazilian Cerrado

2021 ◽  
Vol 42 (5) ◽  
pp. 2825-2838
Author(s):  
Roberta Tavares Moreira ◽  
◽  
Ana Lourdes Arrais de Alencar Mota ◽  
Antonio Carlos Lopes Câmara ◽  
Benito Soto-Blanco ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Negative Correlation ◽  
Clinical Signs ◽  
High Sensitivity ◽  
High Specificity ◽  
Gastrointestinal Parasites ◽  
Brazilian Cerrado ◽  
Selective Treatment ◽  
Helminthic Infection

This study aimed to evaluate the sensitivity and specificity of FAMACHA© method, correlating with packed cell volume (PCV) and egg count (FEC), as well as to evaluate the clinical signs of Haemonchus sp. infection in sheep from Brazilian Cerrado. Over two years (2017 to 2019), 1,435 sheep were subjected to clinical and parasitological evaluations. Sheep from six breeds (Santa Inês, Dorper, White Dorper, Ile de France, Suffolk, and crossbreed) were subdivided into five production categories (pregnant, lactating, non-pregnant/lactating ewes, breeding males, and weaned lambs). Parasitological evaluations included FEC and coproculture. In the clinical evaluation, all sheep underwent determination of the FAMACHA© score and PCV. Haemonchus sp. larvae were predominant in coprocultures of the flocks (76.4%) and in each animal production category evaluated (69.4 to 84.3%). FAMACHA© method showed high sensitivity (70.6%) for evaluating sheep with scores ≥ 3, and PCV < 23%, and high specificity (97.5%) in animals with higher scores (4 and 5), and PCV < 18%. A negative correlation was observed between FAMACHA© scores and PCV (-0.46) and between PCV and FEC (-0.47), while a positive correlation was observed between FAMACHA© scores and FEC (0.22) (p < 0.01). The vast majority of the animals evaluated (54.5%) were clinically resistant to gastrointestinal parasites. Due to the high sensitivity and specificity, we concluded that the method could be a valuable diagnostic alternative and an ancillary tool in the implementation of selective treatment for helminthic infection in sheep from Brazilian Cerrado.

scholarly journals Analysis of polymorphisms in the TPMT gene in children with acute leukemia in the Krasnoyarsk Territory

2018 ◽  
Vol 5 (3) ◽  
pp. 56-59
Author(s):  
A. A. Karnyushka ◽  
T. N. Subbotina ◽  
R. V. Shaikhutdinova ◽  
M. V. Borisova ◽  
S. M. Lobanova ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Acute Leukemia ◽  
Standard Treatment ◽  
Lymphoblastic Leukemia ◽  
Malignant Neoplasms ◽  
Lymphoid Leukemia ◽  
Treatment Protocols ◽  
Variant Alleles ◽  
Tpmt Gene

Introduction.Leukemia accounts for 40 % of all malignant neoplasms under the age of 15 years in children. Determination of the genetic portrait of patients with acute lymphoblastic leukemia (ALL) helps to identify polymorphisms in the genes responsible for the metabolism of drugs included in standard treatment protocols.Materials and methods.51 children with confirmed diagnosis of acute lymphoid leukemia (ALL) were included in the analysis of the frequency of polymorphism of TPMT gene. The detection of polymorphisms TPMT*2, TPMT*3A and TPMT*3 was performed using a set of reagents “AmpliSens® Pyroskrin” & “FARMA-screen-2b”.Results.Polymorphisms in the TPMT gene of 51 patients were found in 6 (11.8 %) children. Of these, 4 patients have variant alleles TPMT*3A and TPMT*3C and 2 patients have only TPMT*3C. Of the 6 patients with TPMT polymorphism, two have a translocation t(12;21).

scholarly journals Vitamin B12 Deficiency Resembling Acute Leukemia: A Case Report

2021 ◽  
Vol 59 (243) ◽  
pp. 1182-1184
Author(s):  
Nisha Sharma ◽  
Saru Kunwar ◽  
Anil Kumar Shrestha
Keyword(s):  
Bone Marrow ◽  
Acute Leukemia ◽  
Vitamin B12 ◽  
Megaloblastic Anemia ◽  
Vitamin B12 Deficiency ◽  
Bone Marrow Aspiration ◽  
Peripheral Blood Smear ◽  
Poor Growth ◽  
B12 Deficiency ◽  
Chronic Fever

Vitamin B12 deficiency in children can cause megaloblastic anemia, poor growth, and increased chances of infections. It is an important reversible cause of bone marrow suppression which at the time of presentation can mimic hematological malignancy. Therefore, it should be considered as a differential diagnosis in cases suspected of acute leukemia. We report a case of 14 months old child who had atypical presentation of vitamin B12 deficiency. He had chronic fever, decreased feeding and increased paleness for one year. Pancytopenia with severe anemia was present along with 19% reactive/atypical cells in peripheral blood smear suggesting acute leukemia. However, bone marrow aspiration and biopsy showed features of megaloblastic anemia. Vitamin B12 level measured was very low and treatment with cyanocobalamin caused drastic improvement in the child’s condition.

scholarly journals Diagnostic value of anti-SA antibodies and antibodies to heterogeneous nuclear ribonucleoprotein K in rheumatoid arthritis

2020 ◽  
pp. 57-63
Author(s):  
M.V. Volkova ◽  
◽  
A.V. Kunder ◽  
D. Roggenbuck ◽  
◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Sensitivity And Specificity ◽  
High Specificity ◽  
Diagnostic Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Diagnostic Characteristics ◽  
Nuclear Ribonucleoprotein

Annotation The aim of the research is to determine the levels and frequency of antibodies popularity to cyclic citrullinated peptide (APCCP), rheumatoid factor (RF), anti-Sa antibodies and antibodies to heterogeneous nuclear ribonucleoprotein K (anti-gRNP K) in rheumatoid arthritis (RA) and to compare their diagnostic characteristics, and also to determine sensitivity and specificity of anti-Sa antibodies, anti-hRNP K in seronegative RA. Material and methods. The study included 270 patients with RA and 50 healthy individuals. The levels of ACCP, anti-Sa, anti-hRNP K were assessed in blood serum samples from patients by enzyme-linked immunosorbent assay using commercial test systems. RF levels were assessed by kinetic nephelometry on automatic analyzer. Results. The levels and popularity frequency of the studied antibodies in patients with RА were significantly higher than in healthy individuals (p <0.05). Tests based on ADCP detection had the highest indices of sensitivity and specificity for RA diagnosis; sensitivity - 78.01%, specificity - 100.00%, and RF - 70.64%, specificity 96.97%. In case of serogegative APCCP and RA RF, diagnostic sensitivity of anti-Sa antibodies determination was 50.00%, specificity was 96.77%, sensitivity of anti-hRNP K antibodies determination was 55.56%, and specificity was 100.00%. Conclusion. Considering high specificity of tests for anti-Sa and anti-hRNP K antibodies in seronegative RA, they can be considered as additional confirmatory diagnostic tests.

Presence of Aspartate Aminotransferase in Peri-Implant Crevicular Fluid With and Without Mucositis

2012 ◽  
Vol 38 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Arturo Sánchez-Pérez ◽  
María José Moya-Villaescusa ◽  
Raúl Guillermo Caffesse
Keyword(s):  
Aspartate Aminotransferase ◽  
Clinical Signs ◽  
High Specificity ◽  
Diagnostic Value ◽  
Pressure Probe ◽  
Clinical Assessments ◽  
Significant Difference ◽  
Crevicular Fluid

The aim of this study was to assess the presence of aspartate aminotransferase (AST) in peri-implant crevicular fluid, with or without clinical signs of mucositis, to determine its predictive diagnostic value, sensitivity, and specificity. The AST levels were determined (at a threshold of 1200 µIU/mL) for 60 clinically successful implants in 25 patients with or without peri-implant mucositis. Samples were taken prior (AST1) to peri-implant probing with a manual constant-pressure probe (0.2 N) and 15 minutes after probing (AST2). Clinical assessments included radiographic determination of preexisting bone loss, probing, and the evaluation of mucositis, plaque, and bleeding upon probing. Analysis was performed at both the level of the implant and the patient as a unit. We detected a significant difference between AST1 and AST2 at both levels. A significant difference was observed at AST1 between implants that bled upon probing and those that did not. However, when we considered the patient as a unit, there were no significant differences. The plaque index was not significant at either level. AST1 had high specificity and positive predictive diagnostic value (80%) for bleeding upon probing. Probing induces a greater release of AST from inflamed tissues compared with healthy tissues in situ but not at the systemic level. At the implant level, the implant position could be responsible for this difference. Aspartate aminotransferase was a reliable predictor of patients with mucositis.

Lateral resolution of the electron microbeam analysis

1978 ◽  
Vol 36 (1) ◽  
pp. 542-543
Author(s):  
H.J. Dudek
Keyword(s):  
Quantitative Analysis ◽  
Depth Resolution ◽  
Lateral Resolution ◽  
Cylindrical Particle ◽  
Correction Procedure ◽  
X Ray ◽  
Element Concentrations ◽  
Microbeam Analysis ◽  
The Matrix

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S

Determination of the phase structure of polymerblends by electron microscopy

1978 ◽  
Vol 36 (1) ◽  
pp. 492-493
Author(s):  
Dr. G. Kaemof
Keyword(s):  
Screw Extruder ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Single Screw Extruder ◽  
Transmission Electron ◽  
The Matrix ◽  
Twin Screw ◽  
Special Case ◽  
Microscopic Investigations

A mixture of polycarbonate (PC) and styrene-acrylonitrile-copolymer (SAN) represents a very good example for the efficiency of electron microscopic investigations concerning the determination of optimum production procedures for high grade product properties.The following parameters have been varied:components of charge (PC : SAN 50 : 50, 60 : 40, 70 : 30), kind of compounding machine (single screw extruder, twin screw extruder, discontinuous kneader), mass-temperature (lowest and highest possible temperature).The transmission electron microscopic investigations (TEM) were carried out on ultra thin sections, the PC-phase of which was selectively etched by triethylamine.The phase transition (matrix to disperse phase) does not occur - as might be expected - at a PC to SAN ratio of 50 : 50, but at a ratio of 65 : 35. Our results show that the matrix is preferably formed by the components with the lower melting viscosity (in this special case SAN), even at concentrations of less than 50 %.

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