scholarly journals Isolation and characterization of bioactive compounds from Euphorbia cotinifolia

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
B. Jayalakshmi ◽  
K. A. Raveesha ◽  
K. N. Amruthesh
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Bioactive Molecules ◽  
Klebsiella Pneumonia ◽  
Human Pathogenic Bacteria ◽  
Isolation And Characterization ◽  
Study Results

Abstract Background Green plants are found to be an effective reservoir for bioactive molecules and can provide appreciable sources of antimicrobial agents. Antibacterial activity of solvent extracts of Euphorbia cotinifolia leaves was tested by agar cup diffusion and broth microdilution methods against some common human pathogenic bacteria viz., Bacillus cereus, Klebsiella pneumonia, Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella typhi. The methanol extract of Euphorbia cotinifolia was subjected to a silica gel column, leading to the isolation of a bioactive compound 1. The structure of compounds was elucidated by spectroscopic techniques and assessed for their antibacterial activity against several human pathogenic bacteria. Results The inhibition zone ranged against some common human pathogenic bacteria was 15.25–19.50 mm, 13.50–19.25 mm, 12–18.50 mm, 15–20 mm, and 13–19 mm for ECMF1, ECMF2, ECMF3, compounds 1, respectively. The MIC was found to be in the range 91–729 μg/ml for the fractions. The inhibition range was recorded between 12–19 and 10–14 mm for methanol and ethyl acetate extracts, respectively. K. pneumoniae, E. aerogenes, and B. subtilis were highly susceptible to methanol extract with the maximum inhibition zone of 19 mm. The MIC of the compound 1 against human pathogens was 78–833 μg/ml. Conclusion The present study results suggest that tested plant extracts have moderate to potent antibacterial activity due to the occurrence of phenols and flavonoids in the extracts. The defensive property of natural antibacterials is mainly due to the presence of these major groups, vitamins, phenols, flavonoids, and carotenoids. In the present study, biologically active diterpene was isolated and the structures of the new diterpenoids isolated from E. cotinifolia were closely related to an ingenol ester.

scholarly journals Characterization of Isolated Antibacterial Compounds from Euphorbia Cotinifolia Methanol Extract

2020 ◽  
Vol 9 (4) ◽  
pp. 1725-1736
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Enterobacter Aerogenes ◽  
Inhibition Zone ◽  
Bioactive Molecules ◽  
Klebsiella Pneumonia ◽  
Spectroscopic Techniques ◽  
Significant Activity

Infectious diseases are emerging at an alarming rate, which is posing a great threat to the population because of the continuous battle with pathogens. .Green plants are rich in bioactive molecules providing valuable sources of antimicrobial compounds. Antibacterial activity of various solvent extracts of E.cotinifolia leaves was screened against some human pathogenic bacteria like Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia, Salmonella typhi, Escherichia coli, and Enterobacter aerogenes by agar cup diffusion and minimum inhibitory concentrations (MIC) determined by micro broth dilution Methanol extract of Euphorbia cotinifolia was separated by silica gel column, leading to the isolation of new compounds and structure was elucidated by spectroscopic techniques. The solvent extracts and newly isolated compounds were tested for their antibacterial activity. Methanol and ethyl acetate recorded significant activity against Enterobacter aerogenes and Bacillus subtilis, which showed a maximum inhibition zone of 17.25 mm. MIC ranged from 0.3- 1.25 mg/mL for methanol and ethyl acetate extracts. The inhibition zone measured against test bacteria was 15.25-19.50 mm, 13.50-19.25 mm, 12- 18.50 mm, 15- 20 mm, and 13- 19 mm in ECMF1, ECMF2, ECMF3, compound 1, respectively, and MIC of the compounds 1 and 2 was 78- 833 µg/ ml and 139- 625 µg/ ml, respectively. The identification of active compounds of methanol extract, which would lead to the discovery of new antimicrobial drugs from the test plant.

scholarly journals TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

2019 ◽  
pp. 69-73
Author(s):  
ANNAMALAI MADURAM ◽  
RAJU KAMARAJ
Keyword(s):  
Antibacterial Activity ◽  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Klebsiella Pneumonia ◽  
Human Pathogens ◽  
Human Pathogenic Bacteria

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce. Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin. Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild. Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

scholarly journals DELONIX REGIA BOJ. EX. HOOK RAFFIN; FAMILY: FABACEAE, BARK METHANOL EXTRACT PHYTOCHEMICAL PROFILE AND POTENTIAL THERAPEUTIC EVALUATION

2020 ◽  
pp. 100-105
Author(s):  
ARPITHA SHIVAMALLU ◽  
SHAILASREE SEKHAR
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Laser Scanning Microscopy ◽  
Charge Ratio ◽  
Delonix Regia ◽  
Human Pathogenic Bacteria ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Mcf 7

Objectives: The aim of this study was to evaluate the antioxidant, anti-inflammatory, and anti-cancer potencies of the Delonix regia bark, a first of its kind. Methods: The bark was extracted sequentially in Soxhlet apparatus with hexane, chloroform, and methanol in the increasing order of polarity. These extracts were subjected to find its antioxidant activity and total phenol content. Antibacterial activity against human pathogenic bacteria was tested. The anti-inflammatory properties were elucidated by its capacity to inhibit 15-lipoxygenase (LOX) and human cyclooxygenase (COX)-2. Cell cytotoxic capacity was evaluated against MCF-7 cells breast cancer cell lines. Results: Liquid chromatography (LC)-Mass Spectroscopy (MS) fingerprint of the methanol extract identified a total of 14 polyphenols, of which five were structurally characterized based on their mass-charge ratio [M-H]− peak, UV-vis absorption in comparison to published data. Antibacterial activity by disk diffusion inhibited human pathogenic bacteria. Bacterial biofilm inhibition capacity of extract (750 mg) imaged by confocal laser scanning microscopy revealed loss of microcolonies. Extract when tested for 15-LOX inhibition exhibited IC50 values of 94.5 ± 1.23 mg.mL−1 by enzyme kinetics studies using spectrophotometric techniques. Similarly, it could inhibit COX-2 enzyme at relatively lower concentrations (32.18 ± 1.91 mg.mL−1). Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay indicating its strong antioxidant property with the capacity to protect DNA. In vitro cytotoxicity was evaluated by 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphynyl tetrazolium bromide assay and apoptosis induced in MCF-7 cells was assessed morphologically. Conclusion: Our data suggest that D. regia bark methanol extract exerts its therapeutic activity for further pharmaceutical evaluations. Further studies are necessary to determine the mechanisms of these pharmacological properties.

scholarly journals Isolation and Antibacterial Activity of Endophytic Bacteria Isolates from Gambir (Uncaria gambir (Hunter) Roxb.)

2019 ◽  
Vol 12 (6) ◽  
pp. 139-146
Author(s):  
Suryani Ompusunggu ◽  
Dwi Suryanto ◽  
Yurnaliza
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
And Staphylococcus Aureus ◽  
Paper Disc

A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir (Hunter) Roxb.), along with its antibacterial extract activity test on clinical pathogenic bacteria has been conducted. Isolation of gambir endophytic bacteria was done in nutrient agar incubated at ambient temperature for 48 hours. Samples of gambir leaves and root was used for source of endophytic bacteria. Bacterial isolate was propagated using Mueller Hinton Agar. Bacterial culture was extracted using methanol as solvent. To test methanol extract activity of bacterial isolates, concentration of 20, 40, 60, 80, and 100% (v/v) were used in paper disc. Pathogenic bacterial isolates such as Escherichia coli, Streptococcus mutans, and Staphylococcus aureus were used for antibacterial test. Inhibition zone formed around paper disc indicated antibacterial extract activity against the bacteria. Endophytic bacterial isolates of gambirwas identified using Vitek Compact 2.0® based on biochemical test. Isolates SO02 and SO03 showed relatively higher antibacterial activity, with inhibition zone of 17 and 30 mm, respectively. Methanol extract of SO02 showed to have higher activity of >10 mm at 100% of extract concentration, compared to that of SO03 which only showed <10 mm) against E. coli.High inhibition against S.mutans and S.aureus was obtained at of 60%. Chemical identification found that methanol extract of SO02 and SO03 showed to have flavonoid and tanning group. Bacterial identification showed that SO02 and SO03 were closely related to Enterobacter cloacae and Gemella morbillorum with similarity of 98 and 86%, respectively. However, accurate species of these two isolates should be determined

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK METANOL BATANG BIDARA LAUT (Strychnos ligustrina) TERHADAP BAKTERI PATOGEN

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Edy Kurniawan ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Klebsiella Pneumonia ◽  
E Coli

Abstract : Strychnos ligustrina stem has been empirically used by the people of West Nusa Tenggara and Bali in the treatment of malaria, tooth ache and diarrhea, but there is no scientific data that supports it. This study aims to determine and prove the antibacterial activity of Strychnos ligustrina methanol extract to pathogenic bacteria in vitro and in vivo. This research is an experimental study conducted by measuring the inhibition zone (mm) growth of pathogenic bacteria, determining minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) in vitro, and determining the percentage of antibacterial activity of methanol extract of S. ligustrina stem in vivo. The experiment was conducted using 4 groups of concentrations of S. ligustrina stem methanol extract in an in vitro study of 25, 50, 75, and 100% with ciprofloxacin as a positive control and aquadest as a negative control. In vivo studies experiments were carried out using 6 treatment groups of test animals male mice Balb / c (Mus musculus). The in vitro test results showed that methanol extract of S. ligustrina stems was able to inhibit the growth of pathogenic bacteria with medium categories of clinical isolates of Staphylococcus aureus and categories of weaks to Klebsiella pneumonia and Escherichia coli isolates. The minimum inhibitory concentration (MIC) for S. aureus and K. pneumonia bacteria isolates was at a concentration of 25% while for E. coli isolates at a concentration of 30%. The methanol extract of the S. ligustrina stem has no killing power against the pathogenic bacteria tested. Antibacterial activity in vivo was able to inhibit the growth of S. aureus pathogenic bacteria by 6.60% (at 25% concentration), 8.62% (at 50% concentration), and 17.31% (at 100% concentration), against K. pneumonia was 11.85% (at 25% concentration), 51.21% (at 50% concentration), and 65.92% (at 100% concentration), against E. coliat 19.18% (at concentration 25%), 29.98% (at 50% concentration), and 40.88% (at 100% concentration). Methanol extract of S. ligustrina stem proved to have antibacterial activity in vitro and in vivo. Key words: Srychnos ligustrina, pathogenic bacteria, antibacterial, in vitro, in Vivo. Abstrak : Strychnos ligustrina secara empiris  telah digunakan oleh masyarakat Nusa Tenggara Barat dan Bali dalam pengobatan penyakit malaria, sakit gigi, dan diare, tetapi belum ada data ilmiah yang mendukung. Penelitian ini bertujuan untuk menentukan dan membuktikan aktivitas antibakteri ekstrak metanol batang bidara laut terhadap bakteri patogen secara in vitrodan in vivo. Penelitian ini merupakan penelitian eksperimental yang dilakukan dengan mengukur zona hambat (mm) pertumbuhan bakteri patogen, menentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) secara in vitro, serta menentukan persentase aktivitas antibakteri ekstrak metanol batang bidara laut secara in vivo. Percobaan dilakukan menggunakan 4 kelompok konsentrasi ekstrak metanol batang bidara laut pada penelitian in vitro yaitu 25, 50, 75, dan 100% dengan ciprofloxacin sebagai kontrol positif serta aquadest sebagai kontrol negatif. Pada penelitian in vivo percobaan dilakukan menggunakan 6 kelompok perlakuan hewan uji mencit jantan galur Balb/c (Mus musculus). Hasil uji in vitro menunjukkan ekstrak metanol batang bidara laut mampu menghambat pertumbuhan bakteri patogen dengan kategori sedang terhadap Staphylococcus aureus isolat klinis dan kategori lemah terhadap Klebsiella pneumonia dan Escherichia coli isolat klinis. Nilai konsentrasi hambat minimum (KHM) untuk isolat bakteri S. aureus dan K. pneumoniae adalah pada konsentrasi 25% sedangkan untuk isolat E. coli pada konsentrasi 30%. Ekstrak metanol batang bidara laut tidak memiliki daya bunuh terhadap bakteri patogen yang diuji. Aktivitas antibakteri secara in vivo mampu menghambat pertumbuhan bakteri patogen S. aureus sebesar 6,60% (pada konsentrasi 25%), 8,62% (pada konsentrasi 50%), dan 17,31% (pada konsentrasi 100%), terhadap K. pneumonia sebesar 11,85% (pada konsentrasi 25%), 51,21% (pada konsentrasi 50%), dan 65,92% (pada konsentrasi 100%),   terhadap E. coli sebesar 19,18% (pada konsentrasi 25%), 29,98% (pada konsentrasi 50%), dan 40,88% (pada konsentrasi 100%). Ekstrak metanol batang bidara laut terbukti memiliki aktivitas antibakteri secara in vitro dan in vivo. Kata kunci: Srychnos ligustrina, bakteri patogen, antibakteri, in vitro, in vivo

Biochemical Composition, Antibacterial and Anti-Biofilm Activities of Indian Medicinal Plants

2020 ◽  
Vol 18 ◽  
Author(s):  
Mulugeta Mulat ◽  
Fazlurrahman Khan ◽  
Archana Pandita
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Chemical Constituents ◽  
Multidrug Resistant ◽  
Chloroform Extract ◽  
Acetone Extract ◽  
Biologically Active ◽  
Ocimum Sanctum ◽  
Agar Disc

Background: Medicinal plants have been used for treatments of various health ailments and the practices as a remedial back to thousands of years. Currently, plant-derived compounds used as alternative ways of treatment for multidrug-resistant pathogens. Objective: In the present study, various parts of six medical plants such as Solanum nigrum, Azadirachta indica, Vitex negundo, Mentha arvensis, Gloriosa superba, and Ocimum sanctum were extracted for obtaining biological active constituents. Methods: Soxhlet method of extraction was used for obtaining crude extracts. Agar disc diffusion and 96-well plate spectroscopic reading were used to detect the extract’s antibacterial and antibiofilm properties. Results: The obtained extracts were tested for antimicrobial and antibiofilm properties at 25 mg/mL concentrations. Maximum antibacterial activity was observed in O. sanctum chloroform extract (TUCE) against Staphylococcus aureus (24.33±1.52 mm), S. nigrum acetone extract (MAAC) against Salmonella Typhimurium (12.6 ± 1.5 mm) and Pseudomonas aeruginosa (15.0 ±2.0 mm). Only TUCE exhibited antibacterial activity at least a minimum inhibitory concentration of 0.781 mg/mL. Better antibiofilm activities were also exhibited by petroleum extracts of G. superba (KAPE) and S. nigrum (MAPE) against Escherichia coli, S. Typhimurium, P. aeruginosa and S. aureus. Moreover, S. nigrum acetone extract (MAAC) and O. sanctum chloroform extract (TUCE) were showed anti-swarming activity with a reduction of motility 56.3% against P. aeruginosa and 37.2% against S. aureus. MAAC also inhibits Las A activity (63.3% reduction) in P. aeruginosa. Conclusion: Extracts of TUCE, MAAC, MAPE, and KAPE were exhibited antibacterial and antibiofilm properties against the Gram-positive and Gram-negative pathogenic bacteria. GCMS identified chemical constituents are responsible for being biologically active.

scholarly journals CONSTITUENTS AND INHIBITORY EFFECT ON HUMAN PATHOGENIC BACTERIA OF THE ROOTS OF SCUTELLARIA BAICALENSIS

2019 ◽  
Vol 57 (1) ◽  
pp. 7
Author(s):  
Duc Long Le ◽  
Huu Tung Nguyen ◽  
Thi Thom Nguyen ◽  
Gyung Ja Choi ◽  
Dinh Hoang Vu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Scutellaria Baicalensis ◽  
Plant Materials ◽  
Chemical Structures ◽  
Human Pathogenic Bacteria ◽  
Antibiotic Effect ◽  
Study Results

Abstract-HCTN_16Methanol extract of the roots of Scutellaria baicalensis effectively inhibited the bacterial growth of human pathogenic bacteria Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 21768 and Bacillus subtilis ATCC 6633 at MICs of 2,000 µg/mL. n-Hexane, ethyl acetate and aqueous residues were prepared by successively partitioning the methanol extract with n-hexane and ethyl acetate. Among them, only ethyl acetate layer showed antibiotic effect; whereas n-hexane and aqueous layers were inactive against tested bacteria. The ethyl acetate residue was fractionated by silica gel column chromatography to afford three flavonoids and an oligosaccharide. Their chemical structures were elucidated as wogonin (SB1), baicalein (SB4), baicalin (SB5) and tetrasaccharide (SB10) on the basis of the analysis of NMR and MS spectroscopic data. The isolates were evaluated for in vitro inhibitory effect against human pathogenic bacteria using micro dilution bioassay method. Baicalein (SB4) showed a broad-spectrum inhibition against various human pathogenic bacteria. In particular, it was found to potently inhibit S. aureus ATCC 6538 and B. cereus ATCC 21768 with MICs of 9.5 and 38 µg/mL, respectively. The study results demonstrated antibiotic effect of the extracts from the roots of S. baicalensis and characterization of compounds isolated from the plant materials.

scholarly journals PG and Research Department of Zoology, Kongunadu Arts and Science College, Coimbatore

2015 ◽  
Vol 2 (2) ◽  
pp. 139-142
Author(s):  
Lekeshmanaswamy M ◽  
Anusiya devi K ◽  
Manish Kumar B ◽  
Vasuki C.A
Keyword(s):  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Maximum Activity ◽  
Klebsiella Pneumonia ◽  
Moderate Activity ◽  
Candida Sp ◽  
Antibacterial Assay ◽  
Padina Gymnospora ◽  
Science College

In this study Gracilaria edilis, sargassam and Padina gymnospora from Raameshwaram sea, Tamil Nadu were collected, identified and tested against various pathogenic bacteria. In Antibacterial assay Acetone shows the maximum number of activity against the Salmonella typi (32mm) length of inhibtions occurred andAcetone shows the minimum activity against Klebsiella pneumonia (18mm) of the inhibition level. Under the Antifungal assay Acetone shows the maximum activity against the penicillium.sp, (10mm) of the inhibition. Acetone shows the moderate activity against the Aspergillus niger (5mm) of inhibition and the minimumactivity in candida sp,. (8mm) of inhibition zone level.

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