scholarly journals Identification and estimation of bioactive constituents Negundoside, Berberine chloride, and Marmelosin by HPLC and HPTLC for development of quality control protocols for Ayurvedic medicated oil formulation

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ajay Kumar Meena ◽  
P. Rekha ◽  
Ayyam Perumal ◽  
R. Ilavarasan ◽  
Ravindra Singh ◽  
...  
Keyword(s):  
Quality Control ◽  
Goat Milk ◽  
Bioactive Compound ◽  
Acid Value ◽  
Physicochemical Analysis ◽  
Bioactive Constituents ◽  
Berberine Chloride ◽  
Hplc Profiles ◽  
Oil Formulation ◽  
Control Protocols

Abstract Background Anu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma. It contains more than 25 herbs and goat milk as per the Ayurvedic Formulary of India (AFI). It strengthens the neck, shoulder, and chest muscles and improves the capacity of sense organs. It delays the aging process and reduces hair fall. Recent studies showed that it is also useful in COVID-19. In the current study, an attempt to develop quality control protocols and evaluate the standardization parameters like refractive index, iodine value, saponification value, peroxide value, acid value, rancidity, HPTLC fingerprint profile along with major bioactive compound and quantification of Berberine chloride, Negundoside, and Marmelosin by HPLC. Establishing quality protocol and standard parameters like physicochemical parameters and estimation of bioactive compounds of this preparation is significant for quality control. Results In this study, HPTLC identifies bioactive chemical compounds like Berberine chloride, Marmelosin, Negundoside, glycyrrhizin, and para hydroxybenzoic acid (PHBA), Lupeol, Embelin, and Solasodine, which were present in the Anu Taila formulation. HPLC was used to estimate the bioactive marker compounds Negundoside, Berberine chloride, and Marmelosin were present in the Anu Taila formulation. The quantitative evaluation of Berberine chloride (0.0013%), Marmelosin (0.0366%), Negundoside (0.0086%) is present in Anu Taila formulation. Conclusion The study reveals that sufficient quality control parameters were followed during the preparation of the formulation. Physicochemical analysis was carried out as per the guidelines of Ayurvedic Pharmacopeia of India. HPTLC and HPLC profiles generated in this particular study can be considered as a preliminary tool ascertaining the authenticity of Anu Taila.

scholarly journals A Review of the Botany, Traditional Use, Phytochemistry, Analytical Methods, Pharmacological Effects, and Toxicity of Angelicae Pubescentis Radix

2020 ◽  
Vol 2020 ◽  
pp. 1-28
Author(s):  
Liu Yang ◽  
Ajiao Hou ◽  
Song Wang ◽  
Jiaxu Zhang ◽  
Wenjing Man ◽  
...  
Keyword(s):  
Quality Control ◽  
Mechanism Of Action ◽  
Analytical Methods ◽  
Lumbar Disc ◽  
Pharmacological Study ◽  
Relevant Information ◽  
Chemical Components ◽  
Pharmacological Effects ◽  
Bioactive Constituents ◽  
Traditional Use

Angelicae Pubescentis Radix (AP), as a traditional Chinese medicine (TCM), has been used for thousands of years in China. In this paper, the botany, traditional use, phytochemistry, analytical methods, quality control, pharmacological effects, and toxicity of AP were reviewed. It can provide a reference for the further research and lay a foundation for the rational clinical application of AP. The relevant information on AP was collected from scientific databases (such as Baidu Scholar, CNKI, Google Scholar, PubMed, Science Direct, Web of Science, and SciFinder Scholar), Chinese herbal classics, Chinese Pharmacopoeia, PhD and MSc dissertations, and so on. The components which have been isolated and identified in AP include coumarins, volatile oils, organic acids, terpenes, polysaccharides, flavonoids, sterols, and trace elements. Most of them were analyzed by HPLC and GC. A pharmacological study shows that the AP has extensive pharmacological effects, including anti-inflammatory, antirheumatism, sedative and hypnotic, neuroprotection, antioxidation, antitumor, and allergy, and it is widely used in the treatment of the rheumatoid arthritis, knee osteoarthritis, lumbar disc, ankylosing spondylitis, headaches, stroke hemiplegia, Alzheimer’s, and arrhythmia. AP is a valuable natural medicinal plant. So far, significant advances have been made in phytochemistry and pharmacology. Some traditional uses have been demonstrated by modern pharmacology. However, the chemical components and pharmacological effects of AP are complex and varied, and there are different standards for the evaluation of its quality and efficacy. The mechanism of action, the structure-activity relationship among the components, and the potential synergistic and antagonistic effects remain to be studied. At the same time, there are few studies on the specific compounds related to its pharmacodynamics. In order to better develop and utilize AP, we should establish a more reasonable, reliable, and accurate quality control standard and focus on the study of bioactive constituents and the demonstration of their mechanism of action.

scholarly journals Activity-Guided Isolation of Bioactive Constituents with Antinociceptive Activity fromMuntingia calaburaL. Leaves Using the Formalin Test

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mohd. Izwan Mohamad Yusof ◽  
Mohd. Zaki Salleh ◽  
Teh Lay Kek ◽  
Norizan Ahmat ◽  
Nik Fatini Nik Azmin ◽  
...  
Keyword(s):  
Formalin Test ◽  
Dose Range ◽  
Late Phase ◽  
Bioactive Compound ◽  
Antinociceptive Activity ◽  
Bioactive Constituents ◽  
Isolation And Identification ◽  
Synergistic Activation ◽  
Identification Processes ◽  
Negative Controls

The present study was conducted to determine the antinociceptive potential of methanol extract ofMuntingia calaburaL. (MEMC) and to isolate and identify the bioactive compound(s) responsible for the observed antinociceptive activity. The MEMC and its partitions (petroleum ether (PEP), ethyl acetate (EAP), and aqueous (AQP) partitions), in the dose range of 100, 500, and 1000 mg/kg, were tested using the formalin-induced nociceptive test. The PEP, which exerted the most effective activity in the respective early and late phase, was further subjected to the fractionation procedures and yielded seven fractions (labelled A to G). These fractions were tested, at the dose of 300 mg/kg, together with distilled water or 10% DMSO (negative controls); morphine and aspirin (positive controls) for potential antinociceptive activity. Of all fractions, Fraction D showed the most significant antinociceptive activity, which is considered as equieffective to morphine or aspirin in the early or late phase, respectively. Further isolation and identification processes on fraction D led to the identification of three known and one new compounds, namely, 5-hydroxy-3,7,8-trimethoxyflavone (1), 3,7-dimethoxy-5-hydroyflavone (2), 2′,4′-dihydroxy-3′-methoxychalcone (3), andcalaburone(4). At the dose of 50 mg/kg, compound3exhibited the highest percentage of antinociceptive activity in both phases of the formalin test. In conclusion, the antinociceptive activity of MEMC involved, partly, the synergistic activation of the flavonoid types of compounds.

scholarly journals Bomb dating, age validation and quality control of age determinations of monodontids and other marine mammals

2014 ◽  
Vol 8 ◽  
Author(s):  
Steven E Campana ◽  
Robert EA Stewart
Keyword(s):  
Quality Control ◽  
Marine Mammals ◽  
Age Determination ◽  
Age Validation ◽  
Age Bias ◽  
Bomb Radiocarbon ◽  
Control Protocols ◽  
The 1960S ◽  
Age Determinations

Methods for confirming the accuracy of age determination methods are reasonably well established in fishes, but the millions of routine age determinations which take place every year require their own quality control protocols. In contrast, methods for ensuring accuracy in age determination of monodontids and other marine mammals are still being developed. Here we review the basis and application of bomb radiocarbon to marine mammal age validation, highlighting its value for providing unambiguous estimates of age for belugas and other long-lived animals which form growth bands. Bomb radiocarbon is particularly useful for marine mammals, given that the age of an individual animal can be determined to within ±1-3 years, as long as it was alive during the 1960s. However, ongoing age determinations require careful monitoring to ensure that age interpretations remain consistent across ages and through time. Quality control protocols using reference collections of ageing material, in conjunction with age bias plots and measures of precision, are capable of detecting virtually all of the systematic ageing errors that often occur once age determinations of an animal become routine.

Quality control and producing areas differentiation of Gardeniae Fructus for eight bioactive constituents by HPLC–DAD–ESI/MS

Phytomedicine ◽  
2014 ◽  
Vol 21 (4) ◽  
pp. 551-559 ◽  
Author(s):  
Xiaoyan Wu ◽  
Yuan Zhou ◽  
Fangzhou Yin ◽  
Chunqin Mao ◽  
Lin Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Bioactive Constituents ◽  
Esi Ms

scholarly journals Automated glycan assembly using the Glyconeer 2.1 synthesizer

2017 ◽  
Vol 114 (17) ◽  
pp. E3385-E3389 ◽  
Author(s):  
Heung Sik Hahm ◽  
Mark K. Schlegel ◽  
Mattan Hurevich ◽  
Steffen Eller ◽  
Frank Schuhmacher ◽  
...  
Keyword(s):  
Quality Control ◽  
Peptide Synthesis ◽  
Medical Practice ◽  
Building Blocks ◽  
Solid Support ◽  
Biological Research ◽  
Structure Activity Relationships ◽  
Rapid Access ◽  
Structure Activity ◽  
Control Protocols

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure–activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.

Marsdenia tenacissima: A Review of Traditional Uses, Phytochemistry and Pharmacology

2018 ◽  
Vol 46 (07) ◽  
pp. 1449-1480 ◽  
Author(s):  
Peile Wang ◽  
Jing Yang ◽  
Zhenfeng Zhu ◽  
Xiaojian Zhang
Keyword(s):  
Quality Control ◽  
Multidrug Resistance ◽  
Control Method ◽  
Food Poisoning ◽  
Bioactive Constituents ◽  
Resistance Reversal ◽  
Wide Clinical Application ◽  
Marsdenia Tenacissima

The stems and roots of Marsdenia tenacissima (Roxb.) Wight et Arn., a traditional Chinese medicine and Dai herbal medicine, have been widely used for the treatment of asthma, trachitis, tonsillitis, pharyngitis, cystitis, pneumonia and drug or food poisoning. Nowadays, the extract of Marsdenia tenacissima, under the trademark of “Xiao-ai-ping”, is widely used in clinic for the treatment of different cancers in China. To date, approximately 196 chemical ingredients covering steroids, triterpenes and organic acids have been identified from different parts of this plant. Steroids are the major characteristic and bioactive constituents of this plant. Modern pharmacology has demonstrated that the crude extracts and steroids have various in vitro and in vivo pharmacological activities, such as multidrug resistance reversal, antitumor, anti-angiogenic, immunomodulation and anti-HIV activities. The multidrug resistance reversal of steroids provided evidence for the use of this herb in clinic. However, despite wide clinical application, clinical trials, quality control method, pharmacokinetic and toxicity research on Marsdenia tenacissima were seldom reported and deserved further efforts. The present review aimed to achieve a comprehensive and up-to-date investigation in ethnopharmacology, phytochemistry, pharmacology, clinical study, pharmacokinetics, toxicology and quality control of Marsdenia tenacissima. In addition, the possible perspectives and trends for future studies of Marsdenia tenacissima have also been put forward. It is believed that this review would provide a theoretical basis and valuable data for future in-depth studies and applications.

scholarly journals Quantification of Saponins in Asparagus racemosus by HPLC-Q-TOF-MS/MS

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Churanya Onlom ◽  
Nitra Nuengchamnong ◽  
Watoo Phrompittayarat ◽  
Waraporn Putalun ◽  
Neti Waranuch ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Dry Weight ◽  
Asparagus Racemosus ◽  
Bioactive Constituents ◽  
Traditional Medicines ◽  
Flight Mass Spectrometry ◽  
Relative Standard ◽  
The One ◽  
Tof Ms

Asparagus racemosus Willd. or Shatavari (Asparagaceae family) is an important medicinal plant in Ayurvedic medicine as a rejuvenate for women. A method for quantitative analysis of saponin glycosides bioactive constituents in A. racemosus is reported. A high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS) method was developed and validated for simultaneous determination of five saponin glycosides, asparacoside, shatavarin IX, shatavarin IV, asparanin A and shatavarin V in A. racemosus extracted with 70% MeOH. The method was validated through intra-and inter-day precision, with the relative standard deviation (RSD) less than 6%, limits of detection (LOD) and limits of quantification (LOQ) <10 and 50 ng, respectively. Overall recoveries ranged from 95% to 105%, with RSD ranging from 0.7% to 4.5%. The method was applied to saponin glycoside contents in the leaves, stems, and roots of A. racemosus sourced from different geographical locations, including four provinces in Thailand, and a sample from India. Saponin glycosides were detected predominantly in the roots, the part used in traditional medicines and these showed wide variations in saponin glycoside profiles from undetectable to 12 mg/g dry weight. The quality control of A. racemosus is crucial for reliable and predictable therapies and only methods like the one developed has the necessary flexibility, sensitivity, accuracy, and selectivity for reliable routine quality control.

scholarly journals Pharmacognostic Screening of Elaeis guineensis ( Aracaceae) Jacq. Oil and its Effect as an Antidote on Cyanide Poisoning

2020 ◽  
Vol 10 (3-s) ◽  
pp. 1-5
Author(s):  
Felix N. Osuala ◽  
Uchenna E. Odoh ◽  
Grace O. Muoneme ◽  
Sylvester C. Ohadoma
Keyword(s):  
Acute Toxicity ◽  
Moisture Content ◽  
Elaeis Guineensis ◽  
Acid Value ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Acute Toxicity Test ◽  
Cyanide Poisoning ◽  
Saponification Value ◽  
Oil Extract

Introduction: Elaeis guinenesis is a perennial monocot belonging to the family Arecaceae. It is the source of the oil commonly called African palm oil or macaw fat which in traditional medicine has many uses Aim: This study is focused on the pharmacognostic screening of oil of Elaeis guineensis, and its antidotal effect on cyanide poisoning. Method: The extracted oil was subjected to various screening technique in order to determine its quality, purity and chemical constituents. The oil was macroscopically examined; acute toxicity test of Elaeis guineensis oil was carried out on rats. The oil was subjected to heating to determine the moisture content. Phytochemical analysis was also carried out on the palm oil extract. The physicochemical analysis was carried to determine the acid value, saponification value, ester value, hydroxyl value and iodine value. The Ld50 for the pure cyanide was carried out on the rats using “Up and Down” method. The antidotal study of Elaeis guineensis oil was carried out on the rats. Result: Macroscopic evaluation showed, the oil was in fresh condition, smooth texture, bright red colour, characteristic taste, oily appearance and a characterictic smell. The Phytochemical analysis showed the presence of flavonoids, phenols, tannins, saponnins, alkaloids, steroids and terpenoids. The physicochemical analysis showed that the oil has an acid value of 31.2, Saponification value of 194.8, Ester value 163.54, Peroxide value of 18.0, Hydroxyl Percentage of 2.07% and Free Fatty Acid of 3.65. The moisture content was calculated to be 0.2%. For the acute toxicity test on the oil using Lorkes method no death was recorded. The LD50 of the cyanide carried out on the rats showed that the lethal dose of cyanide is 5 mg/kg. The antidotal effect of Elaeis guineensis oil showed the absence of death on the group given oil extract alone and the groups that were poisoned and given the oil (antidote) within 4 minutes. Deaths were recorded for the groups that were administered antidotes after 8 minutes. Conclusion: Elaeis guineensis oil has counteracting effect on cyanide poisoning if administered within four minutes of cyanide ingestion.  

Sign in / Sign up

Export Citation Format

Share Document