scholarly journals The keratinolytic bacteria Bacillus cytotoxicus as a source of novel proteases and feather protein hydrolysates with antioxidant activities

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ivana Cavello ◽  
Brenda Bezus ◽  
Sebastián Cavalitto
Keyword(s):  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Industrial Applications ◽  
Protein Hydrolysates ◽  
Submerged Cultures ◽  
Keratinolytic Activity ◽  
Biotechnological Potential ◽  
Optimum Ph ◽  
First Time

Abstract Background Argentina’s geothermal areas are niches of a rich microbial diversity. In 2020, species of Bacillus cytotoxicus were isolated for the first time from these types of pristine natural areas. Bacillus cytotoxicus strains demonstrated the capability to grow and degrade chicken feathers with the concomitant production of proteases with keratinolytic activity, enzymes that have multitude of industrial applications. The aim of this research was to study the production of the proteolytic enzymes and its characterization. Also, feather protein hydrolysates produced during fermentation were characterized. Results Among the thermotolerant strains isolated from the Domuyo geothermal area (Neuquén province, Argentina), Bacillus cytotoxicus LT-1 and Oll-15 were selected and put through submerged cultures using feather wastes as sole carbon, nitrogen, and energy source in order to obtain proteolytic enzymes and protein hydrolysates. Complete degradation of feathers was achieved after 48 h. Zymograms demonstrated the presence of several proteolytic enzymes with an estimated molecular weight between 50 and > 120 kDa. Optimum pH and temperatures of Bacillus cytotoxicus LT-1 crude extract were 7.0 and 40 °C, meanwhile for Oll-15 were 7.0 and 50 °C. Crude extracts were inhibited by EDTA and 1,10 phenanthroline indicating the presence of metalloproteases. Feather protein hydrolysates showed an interesting antioxidant potential measured through radical-scavenging and Fe3+-reducing activities. Conclusion This work represents an initial approach on the study of the biotechnological potential of proteases produced by Bacillus cytotoxicus. The results demonstrated the importance of continuous search for new biocatalysts with new characteristics and enzymes to be able to cope with the demands in the market.

Antioxidant Properties of Microalgae Protein Hydrolysates Prepared by Neutral Protease Digestion

2014 ◽  
Vol 707 ◽  
pp. 149-153 ◽  
Author(s):  
Xiao Hu ◽  
Xian Qing Yang ◽  
Lai Hao Li ◽  
Yan Yan Wu ◽  
Wan Ling Lin ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Hydrolysates ◽  
Neutral Protease ◽  
Degree Of Hydrolysis ◽  
Protease Digestion ◽  
Hydrolysis Of

Microalgae protein hydrolysates (MPH) were obtained by enzymatic hydrolysis of defatted microalgae meal using neutral protease. The protein recovery, degree of hydrolysis, and the antioxidant activities of the hydrolysates were investigated. The results demonstrated that hydrolysates prepared by neutral protease at 50 °C for 4 h exhibited the strongest antioxidant activity. Under these conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and the reducing power of the hydrolysates were 68.3%, 50.8% and 1.303, respectively.

scholarly journals Bioactive Phenolics from Carthamus lanatus L.

2003 ◽  
Vol 58 (9-10) ◽  
pp. 704-707 ◽  
Author(s):  
Rilka Taskova ◽  
Maya Mitova ◽  
Bozhanka Mikhova ◽  
Helmut Duddeck
Keyword(s):  
Chlorogenic Acid ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenging ◽  
Flavonoid Glycosides ◽  
Aerial Parts ◽  
Free Radical Scavenging Assay ◽  
First Time

AbstractTwo flavonoid aglycons, eight flavonoid glycosides, chlorogenic acid and syringin were isolated from aerial parts of Carthamus lanatus. Isorhamnetin 3-O-β-ᴅ-glucoside and chlorogenic acid were found for the first time in the genus Carthamus and respectively, quercimeritrin, astragalin, kaempferol 3-O-β-ᴅ-sophoroside and syringin in the species. The ethyl acetate fraction of the methanol extract exhibited a higher antioxidant activity than the butanol fraction measured by the α,α-diphenyl-β-picrazylhydrazyl (DPPH) free radical scavenging assay. Cytotoxicity and antioxidant activities of the main constituent, luteolin 7-O-β-D-glucoside, were evaluated.

scholarly journals Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

2021 ◽  
Author(s):  
V. Renuka ◽  
Chandragiri Nagaraja Rao Ravishankar ◽  
Elavarasan Krishnamoorthy ◽  
Abubacker Aliyamveetil Zynudheen
Keyword(s):  
Thermal Behavior ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Structural Difference ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

Antioxidative Activity of Protein Hydrolysates from Spanish Mackerel by Alcalase

2011 ◽  
Vol 236-238 ◽  
pp. 2890-2893 ◽  
Author(s):  
Jun Juan Xiong ◽  
Li Jun Ding ◽  
Xue Ling Lai
Keyword(s):  
Linoleic Acid ◽  
Antioxidative Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Hydrolysates ◽  
Antioxidant Peptide ◽  
Spanish Mackerel ◽  
Radical Scavenging Properties ◽  
Linoleic Acid Emulsion

To utilize low-value fish, Spanish mackerel protein was hydrolyzed using alcalase for production of antioxidant peptide. The antioxidant activities of antioxidant peptide from Spanish mackerel protein were evaluated. Results: The radical-scavenging properties on hydroxyl and superoxide of protein hydrolysates were 53.11% and 23.1%. And their reducing power was increased with the peptide content of the hydrolysates. The hydrolysates significantly inhibited lipid peroxidation in linoleic acid emulsion system, and effectively inhibit autoxidation of linoleic acid; and the inhibition ability increased with the increasing of peptide content in hydrolysates. The hydrolysates inhibited the activity of LOX to a certain extent. Conclusion: The hydrolysates from Spanish mackerel protein exhibited high antioxidative activity.

scholarly journals Novel 2-mercaptobenzimidazole derivatives: synthesis and evaluation of its antibacterial and antioxidant activities

2017 ◽  
Vol 6 (3) ◽  
pp. 77-87 ◽  
Author(s):  
Latifa El Ouasif ◽  
Abdelhakim Bouyahya ◽  
Rachid Zniber ◽  
Mostafa El Ghoul ◽  
Redouane Achour ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Biological Evaluation ◽  
Hydrocarbon Chains ◽  
Free Radical Scavenging Assay ◽  
First Time ◽  
Diffusion Assay ◽  
Antioxidant Effects

In the present study, a series of novel 2-mercapto-benzimidazolium were synthesized from 2-mercapto-benzimidazole with hydrocarbon chains. All compounds obtained are reported for the first time, and the structures of these compounds were confirmed by means of 1H-NMR, 13C-NMR and mass spectrometry. The newly synthesized compounds were subjected to in vitro biological evaluation. The antibacterial activity was evaluated will diffusion assay and density optic method. The antioxidant activity was carried out using DPPH free radical scavenging assay. The result indicated that some compounds show convincing antibacterial activities against two microorganisms: Escherichia coli and Staphylococcus aureus. While, these molecules have not shown interesting antioxidant effects.

Antioxidant Activities of Tilapia (Oreochromis niloticus) Protein Hydrolysates-Iron Chelate Based on Properties of Biological Materials

2013 ◽  
Vol 700 ◽  
pp. 263-267
Author(s):  
Xiao Hu ◽  
Sai Rui Ma ◽  
Yan Yan Wu ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Materials ◽  
Protein Hydrolysates ◽  
Iron Chelate ◽  
Binding Rate ◽  
Mass Ratios

Tilapia (Oreochromis niloticus) protein was hydrolysed by papain, and then the tilapia (Oreochromis niloticus) protein hydrolysates (TPH) chelated with ferrous ions at various mass ratios of TPH to FeCl2 (5:1, 10:1 and 20:1) to obtain the biological materials of chelate I, chelate II and chelate III, respectively. The ferrous-binding rate and antioxidant activities of the chelates were investigated. It was found that the ferrous-binding rate increased with the increase of mass ratios (TPH/FeCl2) from 5:1 to 20:1. The DPPH radical scavenging activity and reducing power activity of chelate III were highest in the chelates, and the lipid peroxidation inhibition activity of the chelate III was obviously higher than that of TPH. Compared with TPH, marked changes in microstructure were observed in the biological material of TPH-iron chelate.

scholarly journals A Comparative Study of New Aspergillus Strains for Proteolytic Enzymes Production by Solid State Fermentation

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Gastón Ezequiel Ortiz ◽  
Diego Gabriel Noseda ◽  
María Clara Ponce Mora ◽  
Matías Nicolás Recupero ◽  
Martín Blasco ◽  
...  
Keyword(s):  
Solid State ◽  
Comparative Study ◽  
Solid State Fermentation ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Thermodynamics And Kinetics ◽  
Wide Range ◽  
Optimum Ph ◽  
Hydrolysis Of ◽  
Optimum Ph And Temperature

A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease activity. Biochemical, thermodynamics, and kinetics features such as optimum pH and temperature values, thermal stability, activation energy (Ea), quotient energy (Q10), Km, and Vmax were studied in four enzymatic extracts from the selected strains that showed the highest productivity. Additionally, these strains were evaluated by zymogram analysis obtaining protease profiles with a wide range of molecular weight for each sample. From these four strains with the highest productivity, the proteolytic extract of A. sojae ATCC 20235 was shown to be an appropriate biocatalyst for hydrolysis of casein and gelatin substrates, increasing its antioxidant activities in 35% and 125%, respectively.

scholarly journals Polyketides and Meroterpenes from the Marine-Derived Fungi Aspergillus unguis 158SC-067 and A. flocculosus 01NT-1.1.5 and Their Cytotoxic and Antioxidant Activities

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 415
Author(s):  
Cao Van Anh ◽  
Jong Soon Kang ◽  
Byeoung-Kyu Choi ◽  
Hwa-Sun Lee ◽  
Chang-Su Heo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Spectroscopic Data ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Aspergillus Unguis ◽  
First Time ◽  
Dpph Radical Scavenging

Ten secondary metabolites, including a new grifolin analog, grifolin B (1); a new homovalencic acid derivative, 12-hydroxyhomovalencic acid (7); and a compound isolated from a natural source for the first time (9), along with seven known compounds, grifolin (2), averantin (3), 7-chloroaverantin (4), 1′-O-methylaverantin (5), 7-hydroxy-2-(2-hydroxypropyl)-5-pentylchromone (6), homovalencic acid (8), and bekeleylactone E (10), were isolated from two fungal strains. The structures of 1–10 were identified by detailed analysis and comparison of their spectroscopic data with literature values. Compounds 9 and 10 showed moderate cytotoxic activity against a panel of cancer cell lines (PC-3, HCT-15, MDA-MB-231, ACHN, NCI-H23, NUGC-3), with the GI50 values ranging from 1.1 µM to 3.6 µM, whereas 1 displayed a weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity without cytotoxicity against all tested cell lines.

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