Presence of human papillomavirus DNA in breast cancer patients: SERUM and tissue analysis.

e12582 Background: It has been demonstrated an increased risk of breast cancer (BC) incidence in patients with previous cervical dysplasia, suggesting a role of human papillomavirus (HPV) of cervical lesions in the development of BC. Although, the origin of HPV spreading to the mammary gland and its mechanism of dissemination is not clear. Methods: Seven serum samples from healthy donors and 58 from early BC patients collected pre-surgery were analyzed for the presence of HPV DNA. For 49/58 patients HPV DNA was analyzed also on the primary tumor tissue. 17 patients had luminal A BC (4 relapsed, 13 non relapsed), 16 had luminal B BC (5 relapsed, 11 non relapsed), 13 had triple-negative BC (6 relapsed, 7 non relapsed), 12 had HER2-positive BC (4 relapsed, 8 non relapsed). Circulating DNA was extracted from 500 μl of serum by Qiamp DNA Mini kit (Qiagen, Milan, Italy) and tumor DNA was extracted from at least four 10-micron sections by QIAamp DNA FFPE Tissue Kit (Qiagen, Milan, Italy). Circulating HPV DNA was amplified by a multiplex PCR with HPV E6 or E7 gene-specific primers and the sequence was assessed by a high-throughput MALDI-TOF mass spectrometry-based method. Results: HPV DNA was detected in only 5 serum of BC patients and in none of the healthy controls. 4/5 BC cases had high-risk HPV DNA (type 39,45,52,59) and 1 had type 73 low-risk HPV DNA. 4/5 HPV DNA-positive patients had previously low-grade cervical intraepithelial neoplasia (CIN I) detected by Pap smear. These 5 patients with circulating HPV DNA did not show HPV positivity in the BC tissue. 2 out of 49 cases were positive for universal HPV DNA sequence in tissue and only 1 case showed HPV type 51. No relation was found between HPV infection and tumor subtype or prognosis, neither for HPV DNA positivity between serum and tissue. Conclusions: Our data support the feasibility of HPV DNA detection by liquid biopsy in BC. The presence of circulating HPV could be due to a viral spread from other organs. More data are needed to establish the role of circulating HPV DNA and its potential association with HPV infection of the breast and/or of the cervix. [Table: see text]

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The Relationship between Cocaine Use and Human Papillomavirus Infections in HIV-Seropositive and HIV-Seronegative Women

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2008/587082 ◽

2008 ◽

Vol 2008 ◽

pp. 1-7 ◽

Cited By ~ 11

Author(s):

Howard Minkoff ◽

Ye Zhong ◽

Howard D. Strickler ◽

D. Heather Watts ◽

Joel M. Palefsky ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Pap Smear ◽

Crack Cocaine ◽

Hpv Infection ◽

Cocaine Use ◽

Hpv Dna ◽

Increased Risk ◽

Hiv Seropositive ◽

Oncogenic Hpv

Objective. Animal data suggest that cocaine has an immunosuppressive effect, but no human studies have been conducted to assess the relation of cocaine use with human papillomavirus (HPV) infection, the viral cause of cervical cancer. Since both cocaine use and HPV infection are common among HIV-positive women, we sought to determine whether use of cocaine and/or crack influences the natural history of HPV among women with or at high risk of HIV.Methods. Women enrolled in the Women's Interagency HIV Study (2278 HIV-seropositive and 826 high-risk seronegative women) were examined every six months for up to 9.5 years with Pap smear, collection of cervicovaginal lavage (CVL) samples, and detailed questionnaires regarding health and behavior, including use of crack and cocaine (crack/cocaine). CVLs were tested for HPV DNA by PCR, with genotyping for over forty HPV types.Results. In multivariate logistic regression models, censoring women treated for cervical neoplasia, crack/cocaine use within the last six months was associated with prevalent detection of oncogenic HPV DNA (odds ratio [OR] = 1.30 (1.09–1.55)), and with oncogenic HPV-positive squamous intraepithelial lesions (SIL) (OR = 1.70 (1.27–2.27)), following adjustment for age, race, HIV-serostatus, and CD4+ T-cell count, the number of sexual partners in the past six months, and smoking. In multivariate Cox models crack/cocaine use was also associated with a trend that approached significance in regard to incident detection of oncogenic HPV-positive SIL (HR = 1.51, 95% CI 0.99–2.30), and while the rate of oncogenic HPV clearance was not related to cocaine use, the clearance of any SIL was significantly lower in those with versus those without recent crack/cocaine use (HR = 0.57, 95% CI 0.34–0.97).Conclusions. Cocaine use is associated with an increased risk of detection of both prevalent and incident oncogenic HPV infection, as well as an increased risk of HPV-positive SIL over time.

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Human papillomavirus, coinfection with Schistosoma hematobium, and cervical neoplasia in rural Tanzania

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200307000-00015 ◽

2003 ◽

Vol 13 (4) ◽

pp. 505-509 ◽

Cited By ~ 4

Author(s):

K. U. Petry ◽

U. Scholz ◽

B. Hollwitz ◽

R. Von Wasielewski ◽

C. J.L.M. Meijer

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Invasive Cervical Cancer ◽

Clinical History ◽

Hpv Infection ◽

Separate Analysis ◽

Hpv Dna ◽

Rural Tanzania ◽

Increased Risk ◽

Local Immunosuppression

Cervical cancer is the most common malignant tumor among women in Tanzania and other countries in tropical Africa. Genital schistosomiasis has been proposed as a possible cofactor in the genesis of this malignant disease that might contribute to its high incidence in regions where bilharzias is endemic. One hundred nine Tanzanian patients from an area with endemic bilharzias who were transferred to a gynecologic out-patient clinic were age-matched with 109 German controls. In patients and controls, separate samples were taken for cytologic assessment and human papillomavirus (HPV) DNA detection using the Hybrid Capture 2 assay (HC2) and PCR (GP5+/6 +). Samples that tested positive for HPV DNA with general primers were re-tested with HPV type-specific primers. After application of 3% acetic acid, punch biopsies were taken from any cervical lesion. Patients were interviewed for recent symptoms or clinical history suggestive of bilharzias. Urine samples from all patients were examined for the presence of schistosoma hematobium ova. Additionally six Tanzanian patients with invasive cervical cancer were included for separate analysis. Patients and controls had an identical prevalence of HPV-DNA (21.5%) using HC2. Based on PCR results with general primers, the corresponding prevalence was 34.5% for Tanzanian cases and 26.9% for German controls. A history suggestive of bilharzias and/or active schistosomiasis were associated with a significantly increased risk for infection with high-risk HPV types. We conclude that infection with Schistosoma hematobium seems to favor persistent genital HPV infection either by traumatizing the genital epithelium and/or by local immunosuppression.

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Type-Specific Identification of Genital Human Papillomavirus Infection in Women with Cytological Abnormality

Acta Cytologica ◽

10.1159/000446389 ◽

2016 ◽

Vol 60 (3) ◽

pp. 211-216

Author(s):

Hernán Vargas ◽

Jenny P. Sánchez ◽

Mónica L. Guerrero ◽

Leider T. Ortiz ◽

Dayanne M. Rodríguez ◽

...

Keyword(s):

Human Papillomavirus ◽

Pap Smear ◽

Hpv Vaccination ◽

Hpv Infection ◽

Epidemiological Surveillance ◽

Molecular Technique ◽

Genotype Distribution ◽

Vaccination Programs ◽

Hpv Dna ◽

Cytological Abnormality

Objectives: To estimate the frequency of human papillomavirus (HPV) infection and the genotype distribution of HPV among women with a Pap smear showing atypical squamous cells of undetermined significance (ASC-US) attending the Program for the Detection and Control of Cervical Cancer in Bogotá, Colombia. Study Design: Cervical samples from 200 women with an ASC-US Pap smear were analyzed for the presence of HPV DNA and genotype distribution using a commercial molecular technique (Linear Array®; Roche Molecular Systems, USA). Results: HPV infection was found in 140 women (70%). High-risk HPV types were present in 46.4% of the samples; 16.4% showed a low-risk HPV type, and 37.1% showed both. Of the positive samples, 42.9% were infected with a single viral genotype, whereas 57.1% exhibited multiple HPV infections. The most common HPV genotypes were HPV 16, 53, and 52 with a prevalence of 26.4, 16.4, and 13.6%, respectively. Conclusion: The epidemiological characterization of HPV infections described in this study might guide actions for epidemiological surveillance to strengthen the program in Bogotá and to develop appropriate HPV vaccination programs.

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The Potential Role of Human Papillomavirus Infection in Bell's Palsy: A Hypothesis-Generating Study Based on a Nationwide Cohort

Frontiers in Medicine ◽

10.3389/fmed.2021.616873 ◽

2021 ◽

Vol 8 ◽

Author(s):

Kuan-Ying Li ◽

Mei-Chia Chou ◽

Renin Chang ◽

Hei-Tung Yip ◽

Yao-Min Hung ◽

...

Keyword(s):

Human Papillomavirus ◽

Index Date ◽

Hpv Infection ◽

Bell’S Palsy ◽

Sensitivity Analyses ◽

Chronic Obstructive ◽

Bell's Palsy ◽

Increased Risk ◽

The Impact

Objective: Our purpose was to investigate whether people with a previous human papillomavirus (HPV) infection were associated with an increased risk of Bell's palsy (BP).Methods: By using Taiwan population-based data, patients aged > 18 years with HPV infection (n = 22,260) from 2000 to 2012 were enrolled and compared with control subjects who had never been diagnosed with an HPV infection at a 1:4 ratio matched by sex, age, index date, and co-morbidities (n = 89,040). The index date was the first date of HPV diagnosis. All the patients were tracked until the occurrence of BP. Cox proportional hazards regression was applied to estimate the hazard ratios (HRs) for the development of BP in both groups.Results: The HPV group had 1.25 [95% confidence interval (CI) = 1.03–1.51] times higher risk of BP compared with the non-HPV group after adjusting for sex, age, and co-morbidities. The association of HPV and BP was significant in the sensitivity analyses. In the subgroup analysis, the impact of HPV infection on the risk of BP was more pronounced in the elderly > 50 years [adjusted hazard ratio (aHR) =1.86; 95% CI = 1.37–2.52], hypertension (aHR = 1.65; 95% CI = 1.17–2.31), and chronic obstructive pulmonary disease (aHR = 2.14, 95% CI 1.333.43) subgroups.Conclusions: Patients with HPV infection have a higher risk of subsequent BP compared with non-HPV patients. More rigorous studies are needed to confirm if and how specific HPV genotypes are associated with BP and the possible role of vaccines in disease prevention.

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Human Papillomavirus in Breast Carcinogenesis: A Passenger, a Cofactor, or a Causal Agent?

Biology ◽

10.3390/biology10080804 ◽

2021 ◽

Vol 10 (8) ◽

pp. 804

Author(s):

Rancés Blanco ◽

Diego Carrillo-Beltrán ◽

Juan P. Muñoz ◽

Alejandro H. Corvalán ◽

Gloria M. Calaf ◽

...

Keyword(s):

Breast Cancer ◽

Risk Factor ◽

Human Papillomavirus ◽

High Risk ◽

Experimental Evidence ◽

Potential Risk ◽

Hpv Infection ◽

Breast Carcinogenesis ◽

Tumor Tissues

Breast cancer (BC) is the most commonly diagnosed malignancy in women worldwide as well as the leading cause of cancer-related death in this gender. Studies have identified that human papillomavirus (HPV) is a potential risk factor for BC development. While vaccines that protect against oncogenic HPVs infection have been commercially available, global disparities persist due to their high cost. Interestingly, numerous authors have detected an increased high risk (HR)-HPV infection in BC specimens when compared with non-tumor tissues. Therefore, it was suggested that HR-HPV infection could play a role in breast carcinogenesis in a subset of cases. Additional epidemiological and experimental evidence is still needed regarding the role of HR-HPV infection in the development and progression of BC.

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Human Papillomavirus Is Associated with Breast Cancer in the North Part of Iran

The Scientific World JOURNAL ◽

10.1100/2012/837191 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

Afsaneh Sigaroodi ◽

Seyed Alireza Nadji ◽

Farshad Naghshvar ◽

Rakhshandeh Nategh ◽

Habib Emami ◽

...

Keyword(s):

Breast Cancer ◽

Breast Tumor ◽

Hpv Infection ◽

Test Results ◽

Breast Cancer Patients ◽

Hpv Dna ◽

The North ◽

North Part ◽

Reference Sequences ◽

High Risk Hpv

We have analyzed the possible relevance of HPV infection for breast cancer risk among Iranian women from north part of Iran. Among women with breast cancer, 25.9% had positive test results for HPV DNA in breast tumor samples in contrast to 2.4% of women with noncancer status (P=0.002). The infection of HPV has increased the risk of breast cancer (OR 14.247; 95% CI 1.558–130.284,P=0.019). The high-risk HPV genotypes (types 16 and 18) in samples of breast cancer patients were the predominant types (53.34%). Other genotypes detected in breast cancer were HPV-6, HPV-11, HPV-15, HPV-23, and HPV-124, and one isolate could not be genotyped compared to HPV reference sequences. While the sole detected HPV in control specimens was HPV-124. Our study reveals that HPV infection and age are the risk factors in breast cancer development in the north part of Iran.

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Evidence of High-Risk Human Papillomavirus in Esophageal Cancer in East Azerbaijan Province, Northwest of Iran

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2022/1099477 ◽

2022 ◽

Vol 2022 ◽

pp. 1-5

Author(s):

Zahra Sadeghian ◽

Hossein Bannazadeh Baghi ◽

Vahdat Poortahmasebi ◽

Javid Sadeghi ◽

Alka Hasani ◽

...

Keyword(s):

Esophageal Cancer ◽

Human Papillomavirus ◽

Hpv Infection ◽

Control Group ◽

Case Group ◽

Nested Polymerase Chain Reaction ◽

Hpv Dna ◽

Increased Risk ◽

Northwest Of Iran ◽

East Azerbaijan

Background. Human papillomavirus (HPV) is one of the most important viral agents associated with several classes of cancers in humans. The aim of this study was to investigate HPV in esophageal cancer in the East Azerbaijan province, northwest of Iran. Methods. 140 paraffin-embedded specimens of esophageal tissues were investigated using nested-polymerase chain reaction (nested-PCR) with primer designing for the L1 region of HPV genome. According to the pathological diagnosis, the samples were divided into two groups: 70 patients with esophageal cancer EADC (n = 35) and ESCC (n = 35) as the case group and those without tumour in esophagus tissue as a control (n = 70). Results. HPV DNA was isolated from 20 (28.57%) of the 70 paraffin-embedded tissue specimens of esophagus cancer. Of these, 6 cases (17.14%) of EADC and 14 cases (40%) of ESCC were positive. In contrast, all cases of the control group were negative for the HPV genome. Sequence analysis revealed that HPV types 16 and 18 are the most frequent ones identified in this study. Conclusion. The prevalence of HPV in esophageal cancer can vary depending on the geographical location and other factors. Based on the findings of this study, HPV infection may possibly have contributed to an increased risk of esophageal cancer in a group of patients in Tabriz.

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Detection of human papillomavirus DNA in fine needle aspirates of women with breast cancer

Archive of oncology ◽

10.2298/aoo1202012d ◽

2012 ◽

Vol 20 (1-2) ◽

pp. 12-14 ◽

Cited By ~ 1

Author(s):

Smaroula Divani ◽

Angeliki Giovani

Keyword(s):

Breast Cancer ◽

Human Papillomavirus ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Malignant Tumors ◽

Transmitted Disease ◽

Hpv Infection ◽

Breast Lesions ◽

Hpv Dna ◽

Benign Breast Lesions

Background: HPV infection is the most commonly distributed sexually transmitted disease. Human papillomavirus has also been linked to malignant tumors of many human organs. The presence of viral DNA in breast cancer cells is controversial. The aim of the present study was to investigate the presence of HPV-DNA in a group of Greek women with breast carcinoma. Methods: Liquid cytology specimens from 35 malignant breast cases and 35 cases with benign breast lesions were investigated by PCR (clinical arrays technique). In addition, in situ hybridization was performed on all HPV positive cases. Results: HPV-DNA was detected in 17.14% of the carcinoma cases and HPV16 DNA was present in 83.3% of them. All benign breast lesions were negative for HPV-DNA. Conclusion: Our report confirmed the presence of HPV in breast cancer cells while the most prevalent type was HPV16. More studies are necessary in order to elucidate the pathogenesis of HPV and a possible way of prevention of some breast cancers.

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Cervical Dysplasia and High-Risk Human Papillomavirus Infections among HIV-Infected and HIV-Uninfected Adolescent Females in South Africa

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2014/498048 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

David H. Adler ◽

Melissa Wallace ◽

Thola Bennie ◽

Megan Mrubata ◽

Beau Abar ◽

...

Keyword(s):

High Risk ◽

Pap Smear ◽

Cervical Dysplasia ◽

Pap Test ◽

Hpv Infection ◽

Cross Sectional Study ◽

Cervical Lesions ◽

Cross Sectional ◽

Hpv Dna ◽

Increased Risk

Background.HIV-infected adolescents may be at higher risk for high-grade cervical lesions than HIV-uninfected adolescents. The purpose of this study was to compare the prevalence of high-risk HPV (HR-HPV) infections and Pap smear abnormalities between these two groups.Methods.In this cross-sectional study, we compared the HPV DNA and Pap smear results between 35 HIV-infected and 50 HIV-uninfected adolescents in order to determine the prevalence of HR-HPV genotypes and cervical cytological abnormalities. Comparisons were made using Pearsonχ2and independent-samplest-tests analyses, and associations between demographic and behavioral characteristics and HPV infections were examined.Results.HIV-infected participants were more likely to be infected with any HPV (88.6% versus 48.0%;P<0.001) and with at least one HR-HPV (60.0% versus 24.0%;P=0.001), and to have multiple concurrent HPV infections (68.6% versus 22.0%;P<0.001). HPV 16 and 18 were relatively underrepresented among HR-HPV infections. Abnormal Pap test results were more common among HIV-infected participants (28.8% versus 12.0%;P=0.054). A history of smoking was associated with HR-HPV infection.Conclusions.HIV-infected adolescents have an increased risk of infection with HR-HPV and of Pap test abnormalities. The majority of HR-HPV infections among our participants would not be prevented by the currently available vaccinations against HPV.

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HPV Status and Its Correlation with BCL2, p21, p53, Rb, and Survivin Expression in Breast Cancer in a Chinese Population

BioMed Research International ◽

10.1155/2017/6315392 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Ya-Wen Wang ◽

Kai Zhang ◽

Song Zhao ◽

Yanrong Lv ◽

Jiang Zhu ◽

...

Keyword(s):

Breast Cancer ◽

Survivin Expression ◽

Hpv Infection ◽

Clinicopathological Characteristics ◽

Breast Cancer Patients ◽

P53 Expression ◽

Cancer Pathogenesis ◽

Hpv Status ◽

Hybrid Capture 2

Despite recent evidence, the role of human papillomavirus (HPV) in breast carcinogenesis is controversial. The correlations of HPV infection with the clinicopathological features of breast cancer and the expression of cell cycle/apoptosis-associated proteins have not been well elucidated. In this study, we sought to determine the prevalence of high-risk HPVs (HR-HPVs) infection and BCL2, p21, p53, Rb, and survivin expression in breast cancer patients and to investigate the relationship of HPV with these cancer-related proteins, in an attempt to clarify the potential mechanism of HPV in breast cancer pathogenesis. HPV presence in 81 fresh breast cancer tissues was determined by hybrid capture 2 (HC2) assay, and expression of BCL2, p21, p53, Rb, and survivin was detected by immunohistochemistry. Here we showed that fourteen (17.3%) patients were HR-HPV positive. HPV infection demonstrated no significant correlation with the clinicopathological characteristics of breast cancer. HPV-positive tumors showed significantly higher BCL2 and lower p53 expression than HPV-negative tumors. Expression of p21, Rb, and survivin was not associated with HPV status. Our results suggest a possible role of HR-HPV in breast cancer carcinogenesis, in which BCL2 and p53 may be involved.

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