scholarly journals The Ecdysone-inducible zinc-finger transcription factor Crol regulates Wg transcription and cell cycle progression in Drosophila

Development ◽  
2008 ◽  
Vol 135 (16) ◽  
pp. 2707-2716 ◽  
Author(s):  
N. Mitchell ◽  
N. Cranna ◽  
H. Richardson ◽  
L. Quinn
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Zinc Finger ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Zinc Finger Transcription Factor

scholarly journals The p110 Isoform of the CDP/Cux Transcription Factor Accelerates Entry into S Phase

2006 ◽  
Vol 26 (6) ◽  
pp. 2441-2455 ◽  
Author(s):  
Laurent Sansregret ◽  
Brigitte Goulet ◽  
Ryoko Harada ◽  
Brian Wilson ◽  
Lam Leduy ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Cell Cycle Progression ◽  
S Phase ◽  
Proteolytic Processing ◽  
Experimental Conditions ◽  
Division Rate ◽  
Cycle Progression ◽  
Processing Event

ABSTRACT The CDP/Cux transcription factor was previously found to acquire distinct DNA binding and transcriptional properties following a proteolytic processing event that takes place at the G1/S transition of the cell cycle. In the present study, we have investigated the role of the CDP/Cux processed isoform, p110, in cell cycle progression. Populations of cells stably expressing p110 CDP/Cux displayed a faster division rate and reached higher saturation density than control cells carrying the empty vector. p110 CDP/Cux cells reached the next S phase faster than control cells under various experimental conditions: following cell synchronization in G0 by growth factor deprivation, synchronization in S phase by double thymidine block treatment, or enrichment in G2 by centrifugal elutriation. In each case, duration of the G1 phase was shortened by 2 to 4 h. Gene inactivation confirmed the role of CDP/Cux as an accelerator of cell cycle progression, since mouse embryo fibroblasts obtained from Cutl1z/z mutant mice displayed a longer G1 phase and proliferated more slowly than their wild-type counterparts. The delay to enter S phase persisted following immortalization by the 3T3 protocol and transformation with H-RasV12. Moreover, CDP/Cux inactivation hindered both the formation of foci on a monolayer and tumor growth in mice. At the molecular level, expression of both cyclin E2 and A2 was increased in the presence of p110 CDP/Cux and decreased in its absence. Overall, these results establish that p110 CDP/Cux functions as a cell cycle regulator that accelerates entry into S phase.

scholarly journals MiR-145, miR-133a and miR-133b inhibit proliferation, migration, invasion and cell cycle progression via targeting transcription factor Sp1 in gastric cancer

FEBS Letters ◽  
2014 ◽  
Vol 588 (7) ◽  
pp. 1168-1177 ◽  
Author(s):  
Tianzhu Qiu ◽  
Xin Zhou ◽  
Jian Wang ◽  
Yiping Du ◽  
Jun Xu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Transcription Factor ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Transcription Factor Sp1

scholarly journals Zinc finger of the cerebellum 5 promotes colorectal cancer cell proliferation and cell cycle progression through enhanced CDK1/CDC25c signaling

2019 ◽  
Author(s):  
Abuduzhayier Maimaiti ◽  
Abulaiti Aizezi ◽  
Jianati Anniwaer ◽  
Ayitula Ayitula ◽  
Buhajar Ali ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Zinc Finger ◽  
Cancer Cell ◽  
Cell Cycle Progression ◽  
Colorectal Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Cycle Progression

The bHLH transcription factor gene AtUPB1 regulates growth by mediating cell cycle progression in Arabidopsis

2019 ◽  
Vol 518 (3) ◽  
pp. 565-572 ◽  
Author(s):  
Taotao Li ◽  
Shiyan Yang ◽  
Xinke Kang ◽  
Wei Lei ◽  
Kang Qiao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Cell Cycle Progression ◽  
Bhlh Transcription Factor ◽  
Transcription Factor Gene ◽  
Cycle Progression ◽  
Factor Gene

scholarly journals The TAL1/SCL Transcription Factor Regulates Cell Cycle Progression and Proliferation in Differentiating Murine Bone Marrow Monocyte Precursors

2010 ◽  
Vol 30 (9) ◽  
pp. 2181-2192 ◽  
Author(s):  
Soumyadeep Dey ◽  
David J. Curtis ◽  
Stephen M. Jane ◽  
Stephen J. Brandt
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Transcription Factor ◽  
Dna Binding ◽  
Cell Cycle Progression ◽  
Ex Vivo ◽  
Critical Role ◽  
Sequence Element ◽  
Cycle Progression ◽  
Murine Bone Marrow

ABSTRACT Monocytopoiesis involves the stepwise differentiation in the bone marrow (BM) of common myeloid precursors (CMPs) to monocytes. The basic helix-loop-helix transcription factor TAL1/SCL plays a critical role in other hematopoietic lineages, and while it had been reported to be expressed by BM-derived macrophages, its role in monocytopoiesis had not been elucidated. Using cell explant models of monocyte/macrophage (MM) differentiation, one originating with CMPs and the other from more committed precursors, we characterized the phenotypic and molecular consequences of inactivation of Tal1 expression ex vivo. While Tal1 knockout had minimal effects on cell survival and slightly accelerated terminal differentiation, it profoundly inhibited cell proliferation and decreased entry into and traversal of the G1 and S phases. In conjunction, steady-state levels of p16(Ink4a) mRNA were increased and those of Gata2 mRNA decreased. Chromatin immunoprecipitation analysis demonstrated the association of Tal1 and E47, one of its E protein DNA-binding partners, with an E box-GATA sequence element in intron 4 of the Gata2 gene and with three E boxes upstream of p16(Ink4a). Finally, wild-type Tal1, but not a DNA binding-defective mutant, rescued the proliferative defect in Tal1-null MM precursors. These results document the importance of this transcription factor in cell cycle progression and proliferation during monocytopoiesis and the requirement for direct DNA binding in these processes.

scholarly journals The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11415-11423 ◽  
Author(s):  
Ricardo Medina ◽  
Timothy Buck ◽  
Sayyed K. Zaidi ◽  
Angela Miele-Chamberland ◽  
Jane B. Lian ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Dna Binding ◽  
Zinc Finger ◽  
Histone Gene ◽  
Binding Motif ◽  
Zinc Finger Transcription Factor ◽  
Cell Cycle Regulator

scholarly journals TATA-Binding Protein-Like Protein (TLP/TRF2/TLF) Negatively Regulates Cell Cycle Progression and Is Required for the Stress-Mediated G2 Checkpoint

2003 ◽  
Vol 23 (12) ◽  
pp. 4107-4120 ◽  
Author(s):  
Miho Shimada ◽  
Tomoyoshi Nakadai ◽  
Taka-aki Tamura
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Stress Response ◽  
Cell Growth ◽  
Cell Cycle Progression ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Cycle Progression ◽  
Stress Dependent ◽  
Dt40 Cells

ABSTRACT The TATA-binding protein (TBP) is a universal transcription factor required for all of the eukaryotic RNA polymerases. In addition to TBP, metazoans commonly express a distantly TBP-related protein referred to as TBP-like protein (TLP/TRF2/TLF). Although the function of TLP in transcriptional regulation is not clear, it is known that TLP is required for embryogenesis and spermiogenesis. In the present study, we investigated the cellular functions of TLP by using TLP knockout chicken DT40 cells. TLP was found to be dispensable for cell growth. Unexpectedly, TLP-null cells exhibited a 20% elevated cell cycle progression rate that was attributed to shortening of the G2 phase. This indicates that TLP functions as a negative regulator of cell growth. Moreover, we found that TLP mainly existed in the cytoplasm and was translocated to the nucleus restrictedly at the G2 phase. Ectopic expression of nuclear localization signal-carrying TLP resulted in an increase (1.5-fold) in the proportion of cells remaining in the G2/M phase and apoptotic state. Notably, TLP-null cells showed an insufficient G2 checkpoint when the cells were exposed to stresses such as UV light and methyl methanesulfonate, and the population of apoptotic cells after stresses decreased to 40%. These phenomena in G2 checkpoint regulation are suggested to be p53 independent because p53 does not function in DT40 cells. Moreover, TLP was transiently translocated to the nucleus shortly (15 min) after stress treatment. The expression of several stress response and cell cycle regulatory genes drifted in a both TLP- and stress-dependent manner. Nucleus-translocating TLP is therefore thought to work by checking cell integrity through its transcription regulatory ability. TLP is considered to be a signal-transducing transcription factor in cell cycle regulation and stress response.

Sign in / Sign up

Export Citation Format

Share Document