The kinetics of cell-substratum detachment mediated by trypsin: a comparison of normal and Duchenne fibroblasts

1989 ◽  
Vol 92 (2) ◽  
pp. 257-262
Author(s):  
L.V. Simon ◽  
J.A. Pizzey ◽  
G.E. Jones
Keyword(s):  
Skin Fibroblasts ◽  
Phase Cell ◽  
Normal Individuals ◽  
Significant Difference ◽  
Increased Sensitivity ◽  
Series Of Experiments ◽  
Dmd Gene ◽  
Reported Data ◽  
The Relationship ◽  
Cell Cell

In previous studies of cell-cell and cell-substratum adhesion, we have identified differences in the behaviour between human skin fibroblasts cultured from normal individuals and patients with Duchenne muscular dystrophy (DMD). In these studies, monolayer cultures were dissociated by trypsinization and no detectable difference was noted in the efficiency of cell dissociation between normal and DMD fibroblast cultures. However, a detailed study by Kent has suggested that Duchenne fibroblasts exhibit increased sensitivity to trypsin. We have re-investigated this finding using an assay that directly measures the number of cells remaining attached to a substratum following trypsinization. In a series of experiments using cultures derived from five normal and five DMD individuals, we can detect no significant difference in the trypsin-induced detachment rates between normal and DMD skin fibroblasts. This observation applies to both growth-phase and stationary-phase cell cultures. This inconsistency with previously reported data on the trypsin-sensitivity of DMD cells is considered in terms of the different assays used and the effects of trypsin on cell-cell and cell-substratum adhesion. The relationship between abnormalities in the behaviour of DMD cells and the localization and primary structure of the DMD gene product are also discussed.

The Effect of two different protein sources in the diet upon the incidence and prevalance of lameness in dairy cattle

1989 ◽  
Vol 1989 ◽  
pp. 66-66
Author(s):  
D N Logue ◽  
A Lawson ◽  
D J Roberts ◽  
E A Hunter
Keyword(s):  
Dairy Cattle ◽  
Crude Protein ◽  
Economic Consequences ◽  
Relative Importance ◽  
The West ◽  
Significant Difference ◽  
Series Of Experiments ◽  
Relationship Of ◽  
The Relationship ◽  
Locomotion Score

The importance of lameness in dairy cattle in UK in terms of its economic consequences and welfare considerations is well documented (Russel and others, 1982). While such epidemiological work has identified factors of importance in the aetiology of the various conditions associated with lameness it has been less successful in apportioning their relative importance, particularly for those conditions affecting the hoof. A series of experiments has been conducted at the West of Scotland College over the period 1983 to 1989 mainly investigating the relationship of nutrition and lameness. In one of these Manson and Leaver (1988) found a significant difference in the prevalence of lameness between two groups of cattle fed complete diets containing either 16.1 or 19.8% crude protein. They also found a significant increase in mean locomotion score (LS) and commented that further studies were needed to understand the dietary processes by which the hoof of the cow was predisposed to such problems.The objective of this experiment was to investigate the influence of the type of protein source in the concentrate upon the locomotion of the cows, the incidence and prevalence of lameness and finally on hoof hardness, shape, growth and wear.

Response To Intervention: Research And Practice

2013 ◽  
Vol 6 (3) ◽  
pp. 273
Author(s):  
Carol Hall ◽  
Jamie Mahoney
Keyword(s):  
General Education ◽  
Research Study ◽  
Study Data ◽  
Learning Needs ◽  
Success Rates ◽  
Service Model ◽  
Significant Difference ◽  
Reported Data ◽  
The Relationship ◽  
Demonstration School

Responseto Intervention (RTI) is a service model designed to meet the learning needs ofstudents prior to diagnosis and placement in special education settings. Results of a quantitative quasi-experimentalresearch study to investigate the relationship between the RTI plan andself-reported implementation practices among general education elementaryteachers in a Florida school district using analysis of variance (ANOVA) revealedno significant difference between demonstration school and comparable schoolgeneral education teachers self-reported practices, self-reported implementation success rates, orself-reported data collection responsibilities.Recommendations for professional development opportunities for allteachers, paraprofessionals, and administrators involved in the RTI process basedupon analyzed research study data are included.

scholarly journals mRNA Biomarkers for Detection of Oral Squamous Cell Cancer

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Fatemeh Rashid ◽  
Tahereh Naji ◽  
Abdolreza Mohamadnia ◽  
Naghmeh Bahrami
Keyword(s):  
Early Diagnosis ◽  
Tumor Staging ◽  
Cell Cancer ◽  
Normal Group ◽  
Normal Individuals ◽  
Significant Difference ◽  
Oral Squamous Cell Cancer ◽  
Relationship Of ◽  
The Relationship ◽  
Larger Sample

Objectives: Cancer is one of the main causes of death in the world. Changes in the expression of CK19 and CEA genes in peripheral blood of OSCC patients were examined for early diagnosis. Methodology: The participants were 36 patients and 36 normal individuals. CK19 and CEA of blood serum were measured through Real-Time PCR. The relationship of the biomarkers with tumor staging and cancer development was examined. Results: Comparison of the two groups of participants using t-test indicated no significant difference in terms of mean age. CK19 marker was positive in 19 participants of the patients group (n=36), which meant the sensitivity of the marker was 53%. In addition, the marker was positive in eight participants of the normal group (n=36). CEA marker was positive in 26 participants of the patients group (n=36), which meant the sensitivity of the marker was 72%. Moreover, the marker was positive in 11 participants in the normal group (n=36). Conclusion: In general, the study introduced a screening test for early diagnosis of OSCC. To have evidences with more reliability, future studies should be carried out with larger sample groups.

Auditory Selective Attention in Cerebral-Palsied Individuals

1985 ◽  
Vol 16 (4) ◽  
pp. 260-266 ◽  
Author(s):  
Lee Ann Laraway
Keyword(s):  
Selective Attention ◽  
White Noise ◽  
Normal Subjects ◽  
Auditory Selective Attention ◽  
Normal Individuals ◽  
Significant Difference

The purpose of this study was to determine whether there is a statistically significant difference between the auditory selective attention abilities of normal and cerebral-palsied individuals. Twenty-three cerebral-palsied and 23 normal subjects between the ages of 5 and 21 were asked to repeat a series of 30 items consisting of from 2 to 4 digits in the presence of intermittent white noise. Results of the study indicate that cerebral-palsied individuals perform significantly poorer than normal individuals when the stimulus is accompanied by noise. Noise was not a significant factor in the performance of the normal subjects regardless of age.

Enhancing Spatial Ability Through Sport Practice

2012 ◽  
Vol 33 (2) ◽  
pp. 83-88 ◽  
Author(s):  
David Moreau ◽  
Jérome Clerc ◽  
Annie Mansy-Dannay ◽  
Alain Guerrien
Keyword(s):  
Mental Rotation ◽  
Spatial Representations ◽  
Mental Rotation Test ◽  
Sport Training ◽  
Sport Practice ◽  
Undergraduate University Students ◽  
Before And After ◽  
Cognitive Plasticity ◽  
Reported Data ◽  
The Relationship

This experiment investigated the relationship between mental rotation and sport training. Undergraduate university students (n = 62) completed the Mental Rotation Test ( Vandenberg & Kuse, 1978 ), before and after a 10-month training in two different sports, which either involved extensive mental rotation ability (wrestling group) or did not (running group). Both groups showed comparable results in the pretest, but the wrestling group outperformed the running group in the posttest. As expected from previous studies, males outperformed women in the pretest and the posttest. Besides, self-reported data gathered after both sessions indicated an increase in adaptive strategies following training in wrestling, but not subsequent to training in running. These findings demonstrate the significant effect of training in particular sports on mental rotation performance, thus showing consistency with the notion of cognitive plasticity induced from motor training involving manipulation of spatial representations. They are discussed within an embodied cognition framework.

Relationship between Thyroglobulin and Tumor Detectabilily with Thallium-201 in Differentiated Thyroid Cancer

2000 ◽  
Vol 39 (01) ◽  
pp. 10-15 ◽  
Author(s):  
S. P. Müller ◽  
Ch. Reiners ◽  
A. Bockisch ◽  
Katja Brandt-Mainz
Keyword(s):  
Thyroid Cancer ◽  
Differentiated Thyroid Cancer ◽  
Roc Analysis ◽  
Tsh Suppression ◽  
Significant Difference ◽  
Test Specificity ◽  
The Relationship ◽  
Tumor Scintigraphy ◽  
Tsh Stimulation

Summary Aim: Tumor scintigraphy with 201-TICI is an established diagnostic method in the follow-up of differentiated thyroid cancer. We investigated the relationship between thyroglobulin (Tg) level and tumor detectability. Subject and methods: We analyzed the scans of 122 patients (66 patients with proven tumor). The patient population was divided into groups with Tg above (N = 33) and below (N = 33) 5 ng/ml under TSH suppression or above (N = 33) and below (N = 33) 50 ng/ml under TSH stimulation. Tumor detectability was compared by ROC-analysis (True-Positive-Fraction test, specificity 90%). Results: There was no significant difference (sensitivity 75% versus 64%; p = 0.55) for patients above and below 5 ng/ml under TSH suppression and a just significant difference (sensitivity 80% versus 58%; p = 0.04) for patients above and below 50 ng/ml under TSH stimulation. In 18 patients from our sample with tumor, Tg under TSH suppression was negative, but 201-TICI-scan was able to detect tumor in 12 patients. Conclusion: Our results demonstrate only a moderate dependence of tumor detectability on Tg level, probably without significant clinical relevance. Even in patients with slight Tg elevation 201-TICI scintigraphy is justified.

Plasma Level of IL-1β in Disseminated Intravascular Goagulation

1991 ◽  
Vol 65 (04) ◽  
pp. 364-368 ◽  
Author(s):  
Hideo Wada ◽  
Shigehisa Tamaki ◽  
Motoaki Tanigawa ◽  
Mikio Takagi ◽  
Yoshitaka Mori ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tumor Necrosis Factor ◽  
Plasma Level ◽  
Organ Failure ◽  
Tumor Necrosis ◽  
Mononuclear Cells ◽  
Solid Cancer ◽  
Normal Individuals ◽  
The Relationship ◽  
Necrosis Factor

SummaryThe plasma level of interleukin-1β (IL-1β) was determined in normal individuals, patients with disseminated intravascular coagulation (DIC), patients in the pre-DIC period (within 7 days before the onset of DIC), and non-DIC patients to examine the relationship between DIC and the plasma ILlp level. The plasma IL-1β level was 0-0.085 ng/ml in normal individuals, with little difference being seen according to related age. It was significantly higher in the DIC group (0.19 ± 0.19 ng/ml) than in the pre-DIC group (0.05 ± 0.08 ng/ml) or the non-DIC group (0.09 ± 0.01 ng/ml). The plasma IL-1β level was not markedly elevated in leukemia patients, even in the DIC group, but it was significantly increased in the DIC group of solid cancer patients and was generally elevated in patients with sepsis. It was markedly elevated to 0.39 ± 0.26 ng/ml in patients with organ failure. When mononuclear cells were incubated with lipopolysaccharide, it was found that IL-1β, tumor necrosis factor, and tissue factor (TF) were released into the medium, and there was an increase of TF release from endothelial cells incubated with this medium. These results suggest that the increase in IL-Iβ reflected the activation of monocytes and may be an important factor in DIC and its associated organ failure.

Fecundity and Gonado-somatic Index of Trichiurus lepturus (Linnaeus, 1758) Along the Zamboanga del Norte Coast

2018 ◽  
Vol 6 (7) ◽  
pp. 120
Author(s):  
Ma. Dulce C. Guillena
Keyword(s):  
Sex Ratio ◽  
Stock Assessment ◽  
Chi Square ◽  
Moon Phases ◽  
Ovary Weight ◽  
Moment Coefficient ◽  
Trichiurus Lepturus ◽  
Significant Difference ◽  
The Relationship ◽  
Somatic Index

Gonado-somatic index and fecundity are tools for measuring the sexual maturity and ability of animals to reproduce.  This study investigates the reproduction of Trichiurus lepturus. Specifically, this aimed to determine the sex ratio, the GSI, the relationship between fecundity and total length, fecundity and total weight, fecundity and ovary weight. The Descriptive Method of research was used.  Percentage and chi-square was utilized in determining the percentage of occurrence and sex ratio respectively.   Pearson r Product Moment Coefficient of Correlation was used to determine the relationships of the parameters. The study revealed that females outnumbered males and the sex ratio for different month showed significant difference.  Spawning season was observed to occur in November and December as revealed in its GSI values and it synchronized with the full and new moon phases.  Fecundity is positively correlated with body weight, body size, and ovary weight where ovary weight is observed to be the best index for fecundity.  The results of this study could be used further for formal stock assessment of cutlassfish fishery.

Rendimento de grãos de soja e de milho, no Rio Grande do Sul, não difere entre eventos El Niño Oscilação Sul

Agrometeoros ◽  
2018 ◽  
Vol 26 (1) ◽  
Author(s):  
Ronaldo Matzenauer ◽  
Bernadete Radin ◽  
Alberto Cargnelutti Filho
Keyword(s):  
El Niño ◽  
El Nino ◽  
Southern Oscillation ◽  
Rio Grande ◽  
La Niña ◽  
Rio Grande Do Sul ◽  
La Nina ◽  
Significant Difference ◽  
Income Data ◽  
The Relationship

O objetivo deste trabalho foi avaliar a relação entre o fenômeno El Niño Oscilação Sul - ENOS e o rendimento de grãos de soja e de milho no Rio Grande do Sul e verificar a hipótese de que os eventos El Niño são favoráveis e os eventos La Niña são prejudiciais ao rendimento de grãos das culturas. Foram utilizados dados de rendimento de grãos dos anos agrícolas de 1974/75 a 2016/17, e relacionados com as ocorrências de eventos ENOS. Foram analisados os dados de rendimento observados na colheita e os dados estimados com a remoção da tendência tecnológica. Os resultados mostraram que não houve diferença significativa do rendimento médio de grãos de soja e de milho na comparação entre os eventos ENOS. Palavras-chave: El Niño, La Niña, safras agrícolas. Abstract – The objective of this work was to evaluate the relationship between the El Niño Southern Oscillation (ENSO) phenomenon with the grain yield of soybean and maize in Rio Grande do Sul state, Brazil and to verify the hypothesis that the El Niño events are favorable and the La Niña events are harmful to the culture’s grain yields. Were used data from the agricultural years of 1974/75 to 2016/17, and related to the occurrence of ENOS events. We analyzed income data observed at harvest and estimated data with technological tendency was removed. The results showed that there was no significant difference in the average yield of soybeans and corn in the comparison between events.

Income or Culture? Examining the Determinants of Subjective Wellbeing in an International Sample

2019 ◽  
Author(s):  
Siddharth Garg
Keyword(s):  
Online Survey ◽  
Subjective Wellbeing ◽  
Regression Coefficient ◽  
Marginal Utility ◽  
Economic Class ◽  
Income Groups ◽  
Significant Difference ◽  
The World ◽  
Diminishing Marginal Utility ◽  
The Relationship

Objective: The aim of this paper was to examine the relationship between income, subjective wellbeing, and culture among people from a higher socio-economic class across the world. Rationale: Ed Diener proposed the law of diminishing marginal utility as an explanation for differences in subjective wellbeing among different income groups across different countries (Diener, Ng, & Tov, Balance in life and declining marginal utility of diverse resources, 2009). Thus, people with higher incomes would experience less subjective wellbeing due to income, and culture should emerge as a significant predictor. Method: Data from this study came from another study (https://siddharthgargblog.wordpress.com/2019/07/14/love-for-money/). I used an online survey to collect data on annual income in US dollars, subjective wellbeing (WHO-5), and country of residence (Indicator of Culture). 96 responses (Indians = 24, Foreigners = 72) were entered in IBM SPSS and a regression analysis was conducted. The raw dataset used in this study can be found at https://doi.org/10.6084/m9.figshare.8869040.v1Results: ANOVA showed a significant difference (p < 0.05) between Indians and foreigners on levels of subjective wellbeing. Linear regression shows the regression coefficient of culture to be significant (Beta = -.254, p = .014) but the regression coefficient of income was not found to be significant. The overall model was found to explain 8.2% of the variance in wellbeing.Conclusion: The sample of this study is too small to make any kind of generalization; it does lend a little bit of support to the idea of diminishing marginal utility of income on subjective wellbeing and provides a rationale for further research.

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