Determination of plasma volume in anaesthetized piglets using the carbon                monoxide (CO) method

Based on measurements of the circulating red blood cell volume (VRBC) in seven anaesthetized piglets using carbon monoxide (CO) as a label, plasma volume (PV) was calculated for each animal. The increase in carboxyhaemoglobin (COHb) concentration following administration of a known amount of CO into a closed circuit re-breathing system was determined by diode-array spectrophotometry. Simultaneously measured haematocrit (HCT) and haemoglobin (Hb) values were used for PV calculation. The PV values were compared with simultaneously measured PVs determined using the Evans blue technique. Mean values (SD) for PV were 1708.6 (287.3)ml and 1738.7 (412.4)ml with the CO method and the Evans blue technique, respectively. Comparison of PVs determined with the two techniques demonstrated good correlation ( r = 0.995). The mean difference between PV measurements was -29.9 ml and the limits of agreement (mean difference ±2SD) were -289.1 ml and 229.3 ml. In conclusion, the CO method can be applied easily under general anaesthesia and controlled ventilation with a simple administration system. The agreement between the compared methods was satisfactory. Plasma volume determined with the CO method is safe, accurate and has no signs of major side effects.

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Agreement of Two Different Laboratory Methods Used to Measure Electrolytes

Journal of Laboratory Physicians ◽

10.4103/0974-2727.86843 ◽

2011 ◽

Vol 3 (02) ◽

pp. 104-109 ◽

Cited By ~ 14

Author(s):

Venencia Albert ◽

Arulselvi Subramanian ◽

Kanchana Rangarajan ◽

Ravindra Mohan Pandey

Keyword(s):

Cross Sectional Study ◽

Mean Difference ◽

Universal Method ◽

Limits Of Agreement ◽

Serum Samples ◽

Cross Sectional ◽

Laboratory Methods ◽

Flame Photometer ◽

The Mean

ABSTRACT Aim: The aim of our study was to do an agreement analysis of two different laboratory methods used to measure electrolytes i.e., between the ISE based Beckman Coulter Synchron CX9 PRO Biochemistry analyzer and RAL′s Ion3 Flame Photometer (Técnica para el Laboratorio, Barcelona, Spain), in serum samples. Materials and Methods: This cross sectional study was done over a period of three months from September′09 through December′09 on routine biochemistry samples. A total of 6492 samples were received for routine biochemistry analysis from those 630 blood samples were randomly processed for this study. Two ml of sample was taken in a plain gel tube (LABTECH Disposables, Ahmedabad, India), centrifuged and further processed using both systems within one hour of the sampling to obtain the Na and K concentrations in the samples. The bias and variability of differences in measured values were analyzed according to Bland and Altman method. Results: Flame photometry method has drawbacks such as low throughput, requires manual operation, is a time consuming procedure. Ion selective electrodes technique is a more universal method for the high throughput determination of electrolytes in physiological samples; Beckman Coulter Synchron CX9 PRO is an example of such a system. The mean difference between the two methods (standard minus test) and 95% limits of agreement for sodium in serum was -7.8±17.3 (-42.2 to 26.6) and in urine was -22±41 (-104 to 60). Similarly, the mean difference between the two methods for potassium values in serum was found to be -0.25±0.75 (-1.75 to 1.25) and in urine was -5.3±38.9 (-83.1 to 72.5). With 95% confidence interval, the value of sodium and potassium as determined by both the methods lie between the upper and lower limit showing 95% limits of agreement. Conclusion: Good degree of agreement was seen on comparing the two methods for measuring the electrolytes; the use of Synchron CX9 in place of Flame photometer for electrolyte analysis in serum and urine is justified or use the two interchangeably.

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SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

10.1055/s-0038-1644425 ◽

1987 ◽

Cited By ~ 1

Author(s):

J Grøndahl-HANSEN ◽

N Agerlin ◽

L S Nielsen ◽

K Danø

Keyword(s):

Plasminogen Activator ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Mean Values ◽

Amino Terminal ◽

Type Plasminogen Activator ◽

Healthy Donors ◽

Urokinase Type Plasminogen Activator ◽

The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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TO COMPARE THE EFFECTIVENESS OF NEBULISATION WITH ADRENALINE, 3% HYPERTONIC SALINE AND NORMAL SALINE IN BRONCHIOLITIS IN CHILDREN OF 2 TO 24 MONTHS OF AGE BASED ON CLINICAL SEVERITY SCORE AND NEBULISATION FREQUENCY.

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v5i2.1764 ◽

2021 ◽

Vol 5 (2) ◽

Author(s):

Priyanka Jain ◽

Rakesh Jain

Keyword(s):

Hypertonic Saline ◽

Normal Saline ◽

Mean Difference ◽

Clinical Severity ◽

Research Centre ◽

P Value ◽

College Hospital ◽

Mean Values ◽

Significant Difference ◽

The Mean

Background & Method: We conducted a double blinded study at Index Medical College Hospital & Research Centre, Indore. The sample size was determined to be minimum of 120 cases as based upon previous years admission due to acute bronchiolitis. Initially, 146 cases were included in the study out of which 23 cases dropped out of the study after giving consent by guardian for participation in the study as they left against medical advice from the hospital. Result: The mean difference of CSS between 0 minutes to 60 minutes of nebulisation between groups in all cases was 0.4 ± 0.6, between 60 minutes and 4 hours was 0.8 ± 0.6, between 4 to 8 hours was 0.7 ± 0.6, between 8-12 hours was 0.6 ± 0.4, between 12-24 hours was 1.6 ± 0.9 and between 24-48 hours was 1.9 ± 0.9.The mean values and resultant p-value of ANOVA of various nebulising agents used for improvement in CSS shows significant association between various nebulising agents used along with improvement in CSS at the end of assessment at 48 hours of treatment. Conclusion: This study was conducted to establish the efficacy of each nebulisation agent (i.e. adrenaline, 3% hypertonic saline and normal saline) currently used and compare the outcomes as there is not enough evidence amongst Indian population on level of efficacy of each drug in causing improvement in symptoms and signs in various severities of bronchiolitis in early childhood. Comparison of significant improvement in mean difference in CSS at various intervals in all cases compared between groups by post hoc test revealed non-significant difference (p-value 0.700) between 3% hypertonic saline and normal saline. Keywords: nebulisation, adrenaline, bronchiolitis & clinical.

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Comparison of Emission Levels of Motor Cars, Motorcycles, and Tricycles Using Petrol Engines in Southwestern Nigeria

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.900.183 ◽

2021 ◽

Vol 900 ◽

pp. 183-187

Author(s):

Odunlami Olayemi Abosede ◽

Akeredolu Funso Alaba

Keyword(s):

Carbon Dioxide ◽

Carbon Monoxide ◽

Carbon Dioxide Emissions ◽

Mean Value ◽

Southwestern Nigeria ◽

Mean Values ◽

Mobile Sources ◽

Southwest Nigeria ◽

The Mean ◽

European Standards

The emissions of carbon monoxide, carbon dioxide, and hydrocarbon from four stroke-powered motorcars and two stroke-powered motorcycles and tricycles in Southwest Nigeria were examined using an automotive 4-gas analyer. Results show that tricycles produced more hydrocarbon and carbon monoxide emissions than motorcycles, while motorcycles emitted more of these pollutants than the gasoline fueled motor cars. (The gasoline fueled motorcars produced lowest hydrocarbon and carbon monoxide while the tricycles produced the highest hydrocarbon and carbon monoxide emissions). On the contrary, motor cars had the highest mean value of carbon dioxide followed by the motorcycles, while tricycles had the least. This could be attributed to the presence of the catalytic converters in some of the motor cars oxidizing carbon monoxide to carbon dioxide. The mean values of hydrocarbon, carbon monoxide and carbon dioxide emissions from motorcars are 630ppm, 10200ppm and 59900ppm. This is much higher than the NESREA (National Environmental standards and Regulations Enforcement Agency) standards as well as Euro II and Euro III (European standards) for vehicular emission. The mean values for hydrocarbon, carbon monoxide and carbon dioxide emissions from motorcycles and tricycles are (2150ppm, 21530ppm and 31200ppm) and (2820ppm, 24880ppm and 38710ppm) respectively. These results do not comply with Nigeria and European emission standards for hydrocarbon, and carbon monoxide. Tricycles and motorcycles account for higher concentrations of hydrocarbon and carbon monoxide pollutants from mobile sources, while they emit carbon dioxide minimally.

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pH of exudate test in the physiological quality of crambe seeds

Ciência Rural ◽

10.1590/0103-8478cr20141838 ◽

2016 ◽

Vol 46 (6) ◽

pp. 1014-1018 ◽

Cited By ~ 4

Author(s):

Charline Zaratin Alves ◽

Lennis Afraire Rodrigues ◽

Carlos Henrique Queiroz Rego ◽

Josué Bispo da Silva

Keyword(s):

Pearson Correlation ◽

Raw Material ◽

Mean Values ◽

Tukey Test ◽

Physiological Quality ◽

The Subject ◽

The Mean ◽

C 30

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

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Evaluation of Inter-System Variability in Liver Stiffness Measurements

Ultraschall in der Medizin - European Journal of Ultrasound ◽

10.1055/s-0043-124184 ◽

2018 ◽

Vol 40 (01) ◽

pp. 64-75 ◽

Cited By ~ 14

Author(s):

Giovanna Ferraioli ◽

Annalisa De Silvestri ◽

Raffaella Lissandrin ◽

Laura Maiocchi ◽

Carmine Tinelli ◽

...

Keyword(s):

Shear Wave ◽

Liver Stiffness ◽

Shear Wave Elastography ◽

Mean Difference ◽

Observer Agreement ◽

Concordance Correlation ◽

Limits Of Agreement ◽

Inter Observer Variability ◽

System Variability ◽

The Mean

Abstract Aim The primary aim of this study was to determine the inter-system variability of liver stiffness measurements (LSMs) in patients with varying degrees of liver stiffness. The secondary aim was to determine the inter-observer variability of measurements. Materials and Methods 21 individuals affected by chronic hepatitis C and 5 healthy individuals were prospectively enrolled. The assessment of LSMs was performed using six ultrasound (US) systems, four of which with point shear wave elastography (p-SWE) and two with 2 D shear wave elastography (2D-SWE) systems. The Fibroscan (Echosens, France) was used as the reference standard. Four observers performed the measurements in pairs (A-B, C-D). The agreement between different observers or methods was calculated using Lin’s concordance correlation coefficient. The Bland-Altman limits of agreement (LOA) were calculated as well. Results There was agreement above 0.80 for all pairs of systems. The mean difference between the values of the systems with 2D-SWE technique was 1.54 kPa, whereas the maximum mean difference between the values of three out of four systems with the pSWE technique was 0.79 kPa. The intra-patient concordance for all systems was 0.89 (95 % CI: 0.83 – 0.94). Inter-observer agreement was 0.96 (95 % CI: 0.94 – 0.98) for the pair of observers A-B and 0.93 (95 % CI: 0.89 – 0.96) for the pair of observers C-D. Conclusion The results of this study show that the agreement between LSMs performed with different US systems is good to excellent and the overall inter-observer agreement in “ideal conditions” is above 0.90 in expert hands.

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The Determination of the Probabilistic Properties of Velocities and Accelerations in Kinematic Chains With Uncertainty

15th Design Automation Conference: Volume 2 — Design Optimization ◽

10.1115/detc1989-0094 ◽

1989 ◽

Author(s):

S. J. Lee ◽

B. J. Gilmore

Keyword(s):

Effective Length ◽

Radial Clearance ◽

Probabilistic Design ◽

Link Length ◽

Mean Values ◽

Kinematic Chains ◽

Analysis Package ◽

The Mean ◽

Linkage Model

Abstract A probabilistic model and methods to determine the means and variances of the velocity and acceleration of stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link’s effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data is the nominal linkage model and statistical information. The “effective link length” model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper solves the higher-order kinematics problem for the probabilistic design analysis of stochastically defined mechanisms.

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Blood and plasma volume in adult males

Journal of Applied Physiology ◽

10.1152/jappl.1962.17.2.195 ◽

1962 ◽

Vol 17 (2) ◽

pp. 195-198 ◽

Cited By ~ 22

Author(s):

Marvin J. Yiengst ◽

Nathan W. Shock

Keyword(s):

Plasma Volume ◽

Age Groups ◽

Dilution Method ◽

Total Blood Volume ◽

Adult Males ◽

Age Changes ◽

Total Blood ◽

Mean Values ◽

The Mean ◽

Blood Volumes

Total blood volumes were estimated in 94 normal ambulatory males, aged 19–95 years, by the T-1824 dye dilution method. There were no significant age changes when results were expressed in terms of either body weight or surface area. The mean values for individual age groups between 40'49 and 80'95 years varied between 46.7 ± 1.5 and 51.5 ± 1.5 ml/kg body wt. for plasma volume and between 75.1 ± 2.0 and 82.3 ± 2.0 ml/kg body wt. for total blood volume. Submitted on October 16, 1961

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Colorimetric Method for Rapid Determination of Serum Arginase

Clinical Chemistry ◽

10.1093/clinchem/13.10.900 ◽

1967 ◽

Vol 13 (10) ◽

pp. 900-908 ◽

Cited By ~ 19

Author(s):

Brigitta Mellerup

Keyword(s):

Rapid Determination ◽

Colorimetric Method ◽

Normal Range ◽

Clinical Routine ◽

Mean Values ◽

Enzymatic Formation ◽

Significant Difference ◽

The Mean ◽

Normal Men

Abstract A method for the determination of serum arginase is given which combines the enzymatic formation of urea with the sensitive method of Coulombe (1) for measuring this substance. This procedure allows more accurate determinations in the normal range than do previous methods described and is convenient for clinical routine. Significant difference is found between the mean values of normal men and women, 3.9 units/L. for the former and 2.9 units/L. for the latter.

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Determination of carcinoembryonic antigen and cancer antigen values with the radioimmunoassay method in healthy females dogs

Veterinární Medicína ◽

10.17221/6810-vetmed ◽

2013 ◽

Vol 58 (No. 5) ◽

pp. 277-283 ◽

Cited By ~ 9

Author(s):

V. Ledecky ◽

A. Valencakova-Agyagosova ◽

J. Lepej ◽

Z. Frischova ◽

S. Hornak ◽

...

Keyword(s):

Carcinoembryonic Antigen ◽

Statistical Significance ◽

Average Weight ◽

Cancer Antigen ◽

Cancer Markers ◽

Mean Values ◽

The Mean ◽

Radioimmunoassay Method ◽

Healthy Females

The aim of this study was to determine reference values of carcinoembryonic antigen and cancer antigen in 32 clinically healthy bitches. The average age of the bitches in each group was as follows: small breeds 3.50 ± 2.30, medium breeds 3.83 ± 3.21, large breeds 6.00 ± 3.22 and giant breeds 2.40 ± 2.43. The average weight in each group was as follows: 1st group 7.94 kg ± 1.84, 2nd group 22.38 kg ± 2.77, 3rd group 35.94 kg ± 7.16, and 4th group 52.75 kg ± 5.04. The cancer markers were determined using human kits. The mean values of the carcinoembryotic antigen markers ± SD were as follows: 1st group 0.18 ± 0.03, 2nd group 0.20 ± 0.03, 3rd group 0.22 ± 0.01, 4th group 0.18 ± 0.04. The statistical significance for the carcinoembryonic antigen markers was P = 0.0042**. The values of cancer antigen markers ± SD were: 4.90 ± 1.04, 4.80 ± 1.13, 5.90 ± 1.22, and 4.72 ± 0.97, respectively. The cancer antigen values were statistically insignificant (P = 0.1762). Based on obtained values of the mean 95%, we expect a standard for carcinoembryonic antigen of 0.00–0.23 ng/ml and for cancer antigen 0.0–7.00 IU/ml. The results of the present study show that it is possible to use human kits for the determination of carcinoembryonic antigen and cancer antigen in clinically healthy bitches using the radioimmunoassay method.

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