Effects of feeding graded levels of whole cottonseed on haemato-biochemical parameters of red Sokoto bucks

Whole cottonseed (WCS) is an important source of protein for ruminants, however, it contains a polyphenolic secondary metabolite gossypol which may reduce its palatability and cause pathophysiological effects. The study was aimed at evaluating the effects of feeding graded levels of WCS on the haemato-biochemical parameters of Red Sokoto bucks (RSB). After a 14-day pre-treatment period, 20 bucks (n = 5) were assigned for 90 days to one of four isonitrogeneous treatments: control (A); 0 % (B); 15 % (C); 30 % and (D); 45 %. Blood was collected for Packed Cell Volume (PCV) and serum analyses at days 0, 45 and 90 of the experimental period for concentrations of urea, creatinine, total protein, albumin, globulin and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The PCV was decreased in groups fed above 15 % of WCS on days 45. There were no significant (P > 0.05) differences in globulin concentration, alanine aminotransferase and alkaline phosphatase activities but aspartate transferase activity was higher (p < 0.05) in group D (45% WCS) compared to the various treatment groups. The urea concentration was higher (p < 0.05) in groups B and D at day 45 compared to the control group. The creatinine concentration was higher (p < 0.05) in group D at days 45 and 90 compared to the control group. The serum total protein and albumin concentration were higher (p < 0.05) in groups C and D at day 45 compared to the control group. Our finding revealed that prolonged feeding of bucks above 30 % WCS for more than 60 days exerted considerable deleterious and adverse effects on blood constituents leading to aneamia, hyper-cretenaemia, ureamia, increased levels of total protein, albumin, aspartate transferase activity and death. Keywords: Biochemical parameters, Bucks, Gossypol, Serum, Whole cotton seed

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Antioxidative Role of Hatikana (Leea macrophyllaRoxb.) Partially Improves the Hepatic Damage Induced by CCl4in Wistar Albino Rats

BioMed Research International ◽

10.1155/2015/356729 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 8

Author(s):

Samina Akhter ◽

Md. Atiar Rahman ◽

Jannatul Aklima ◽

Md. Rakibul Hasan ◽

J. M. Kamirul Hasan Chowdhury

Keyword(s):

Alkaline Phosphatase ◽

Liver Injury ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Biochemical Parameters ◽

Lipid Profiles ◽

Scavenging Effect ◽

Serum Aspartate Aminotransferase

This research investigated the protective role ofLeea macrophyllaextract on CCl4-induced acute liver injury in rats. Different fractions ofLeea macrophylla(Roxb.) crude extract were subjected to analysis for antioxidative effects. Rats were randomly divided into four groups as normal control, hepatic control, and reference control (silymarin) group and treatment group. Evaluations were made for the effects of the fractions on serum enzymes and biochemical parameters of CCl4-induced albino rat. Histopathological screening was also performed to evaluate the changes of liver tissue before and after treatment. Different fractions ofLeea macrophyllashowed very potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, FeCl3reducing effect, superoxide scavenging effect, and iron chelating effect. Carbon tetrachloride induction increased the level of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and other biochemical parameters such as lipid profiles, total protein, and CK-MB. In contrast, treatment ofLeea macrophyllareduced the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities as well as biochemical parameters activities.L. macrophyllapartially restored the lipid profiles, total protein, and CK-MB. Histopathology showed the treated liver towards restoration. Results evidenced thatL. macrophyllacan be prospective source of hepatic management in liver injury.

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Acute Toxicity of Quercetin From Onion Skin in Mice

Pharmaceutical and Biomedical Research ◽

10.18502/pbr.v6i4.5113 ◽

2021 ◽

Author(s):

Nathan Isaac Dibal ◽

Sani Hyedima Garba ◽

Tamunotonye Watson Jacks

Keyword(s):

Alkaline Phosphatase ◽

Acute Toxicity ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Renal Tubule ◽

Serum Alkaline Phosphatase ◽

Control Group ◽

Wide Range ◽

Two Phases

Background: Quercetin is the most abundant flavonoid molecule, is widely distributed in the plant kingdom, and has a wide range of uses. Objectives: This study aimed to determine the LD50 of quercetin from onion (Allium cepa) skin (QOS) and its effect on the livers and kidneys of mice. Methods: This study consisted of two phases. In phase one, 9 mice BALB/c were divided into three groups of three mice each. The mice in each group received QOS at 10 mg/kg, 100 mg/kg, and 1000 mg/kg, respectively, and were monitored for 24 h for any signs of toxicity or mortality. In phase two, three mice were divided into three groups of one mouse each. Each mouse received QOS at 1600 mg/kg, 2900 mg/kg, and 5000 mg/kg. The mice were observed for 24 h for any signs of toxicity or mortality. Results: A significant increase was observed in serum albumin, total protein, and aspartate aminotransferase (AST) levels in mice that received 10 mg/kg, 100 mg/kg, and 1000 mg/kg QOS. A significant decrease in serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), cholesterol, creatinine, urea, and the electrolyte was noticed in mice that received QOS at 10 mg/kg, 100 mg/kg, and 1000 mg/kg when compared with the control group. The livers of mice that received 1600 mg/kg and 2900 mg/kg QOS showed hemorrhage and enlarged sinusoids along with a distortion of the renal tubule and aggregation of lymphocytes within the kidneys. Conclusion: The LD50 of QOS was 3807 mg/kg in mice. QOS above 1000 mg/kg led to a distortion of the hepatocytes and renal tubule with an increase in serum AST, ALT, and creatinine, suggesting that QOS could be toxic at 1000 mg/kg and above.

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Effects Of High Dosage of Codeine - Containing Cough Syrup Administration on Some Biochemical Parameters of Liver in Albino Rats

Journal of Biochemistry, Microbiology and Biotechnology ◽

10.54987/jobimb.v9i2.614 ◽

2021 ◽

Vol 9 (2) ◽

pp. 30-33

Author(s):

Aishatu Muhammad Bello ◽

Ramlatu Musa Adam ◽

Fatima Umar Maigari ◽

Idi Jalil James ◽

Abubakar Aisami

Keyword(s):

Alkaline Phosphatase ◽

Pain Management ◽

Total Protein ◽

Protein Level ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Biochemical Parameters ◽

Albino Rats ◽

Total Protein Level ◽

Cough Syrup

The study aimed to determine the effect of a high dosage of codeine-containing cough syrup administration on some biochemical parameters of the liver in albino rats. Codeine at 80 mg/kg/day, 160 mg/kg/day, 240 mg/kg/day, 320 mg/kg/day cough syrup were administered orally to albino rats for 21 days, biochemical parameters were analyzed for the activities of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Bilirubin, Total protein and Albumin. Results obtained revealed that a high dosage of codeine administration significantly increased plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and albumin while it reduced total protein level when compared with the control rats. The study confirmed the risk of increased hepatotoxicity due to a high dosage of codeine administration. Although codeine is reported to be effective in pain management, its toxicity should be kept in mind.

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Effect of Xanthohumol Added to Diet on Blood Biochemistry in Japanese Quails

Asian Journal of Agriculture and Food Sciences ◽

10.24203/ajafs.v9i6.6815 ◽

2021 ◽

Vol 9 (6) ◽

Author(s):

Zuzana Lacková ◽

František Zigo ◽

Zuzana Farkašová

Keyword(s):

Enzyme Activity ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Biochemical Parameters ◽

Blood Biochemistry ◽

Control Group ◽

Japanese Quails ◽

Experimental Group ◽

Alt Alanine Aminotransferase

In this paper, we monitored to effect of xanthohumol added to diet on blood biochemistry in Japanese quails. Forty Japanese quails breeds lines Pharaoh were included in the experiment. The quails were randomly divided into two groups: one control and one experimental group with supplementation by xanthohumol in feed. In the evaluation of biochemical parameters, we focused on total protein, albumin, globulin, glucose, cholesterol and enzyme activity of AST (Aspartate aminotransferase), ALT (Alanine aminotransferase) and ɤ-GT (ɤ-Glutamyltransferase). Statistical comparisons were made between group with supplementation by xanthohumol in feed and the control group. Total protein and albumin levels were significantly differed between groups (P <0.05). A significant decrease in AST activity (P <0.05) was observed in supplementation group relative to control group.

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Biochemical parameters of blood of calves when using a probiotic based on the strain enterococcus faecium l-3

International bulletin of Veterinary Medicine ◽

10.17238/issn2072-2419.2020.1.88 ◽

2020 ◽

Vol 1 ◽

pp. 88-92

Author(s):

M.N. Lebedev ◽

◽

S.P. Kovalev ◽

Keyword(s):

Alkaline Phosphatase ◽

Blood Serum ◽

Alanine Aminotransferase ◽

Enterococcus Faecium ◽

Biochemical Parameters ◽

Control Group ◽

Amylase Level ◽

Serum Amylase Level ◽

Positive Effect ◽

Experimental Group

In this paper, we studied the effect of a probiotic, based on the Enterococcus Faeci-um L-3 strain on 20 black-motley calves. The effect of the drug on their biochemical parameters of blood serum were studied. The authors found that this drug, which was fed to calves up to 45 days of age, had a positive effect on the biochemical parameters of blood and on the total animal organism.. So, at 14 days of age, blood serum parame-ters in calves, not receiving the drug, such as: bilirubin, alkaline phosphatase, alanine aminotransferase and aspartate aminotrans-ferase levels were significantly higher than in calves that received probiotic, which indi-cates possible dystrophic changes in the liv-er. Also, at 14 days of age in cows, reciev-ing the drug , the blood serum amylase level was significantly higher than of the calves, not receiving the drug. By the age of 30 days, the tendency to a higher content of bilirubin, alkaline phosphatase, alanine ami-notransferase and aspartate aminotransferase in the blood serum, persisted in the calves of the control group, compared with the ani-mals of the experimental group. By the age of 45 days, the levels of bilirubin, alanine aminotransferase and alkaline phosphatase were also significantly higher in control group of animals, than in experimental group and were 5.0 ± 0.56 μmol / L and 3.72 ± 0.4 μmol / L , 30.66 ± 2.9 IU / L and 16.52 ± 1.2 IU / L, 345.7 ± 30.7 and 226.6 ± 24.1, respectively.The results obtained, indicated that regular feeding of a probiotic based on Enterococcus Faecium L-3 to calves of the milky period, contributes to the normaliza-tion of biochemical parameters and greater resistance to gastrointestinal disorders.

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Pregnancy-associated changes of serum biochemical values in Lipizzaner broodmares

Acta Veterinaria Hungarica ◽

10.1556/004.2015.028 ◽

2015 ◽

Vol 63 (3) ◽

pp. 303-316 ◽

Cited By ~ 7

Author(s):

Boglárka Vincze ◽

Orsolya Kutasi ◽

Ferenc Baska ◽

Ottó Szenci

Keyword(s):

Alkaline Phosphatase ◽

Glutamate Dehydrogenase ◽

Reference Values ◽

Total Protein ◽

Biochemical Parameters ◽

Clinical Chemistry ◽

Venous Blood ◽

Serum Triglyceride ◽

Control Group ◽

Aspartate Transaminase

The aims of this study were to detect physiological changes in blood biochemical parameters throughout gestation, to compare the findings in nonpregnant and pregnant Lipizzaner mares in early-mid and late pregnancy, and to provide reference values for clinical chemistry parameters in this horse breed. A total of 136 venous blood samples were collected from 20 pregnant and 10 nonpregnant (control) asymptomatic Lipizzaner broodmares for biochemical analyses. Twelve parameters (albumin, total protein, urea, triglycerides, glucose, creatinine, alkaline phosphatase, aspartate transaminase, glutamate dehydrogenase, gammaglutamyltransferase, creatine kinase and lactate dehydrogenase) were measured. For the statistical analyses, correlation, analysis of variance and Kruskal–Wallis H-test were used to evaluate the possible associations between parameters. Serum triglyceride levels proved to be significantly different in pregnant mares compared to the control group. Total protein and urea levels significantly decreased, while glucose, triglyceride and glutamate dehydrogenase values increased from approx. the fifth month of gestation until parturition. Four biochemical parameters (albumin, aspartate transaminase, total protein and urea) were lower and three other variables (glucose, alkaline phosphatase and creatinine) were significantly higher in late-term pregnant mares than in mares in early or mid-gestation. It is concluded that reference values not only reflect the species, breed and sex but also the reproductive status of animals.

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Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

Human & Experimental Toxicology ◽

10.1177/0960327110368693 ◽

2010 ◽

Vol 30 (1) ◽

pp. 25-33 ◽

Cited By ~ 1

Author(s):

SE Atawodi ◽

AC Ene ◽

DA Ameh

Keyword(s):

Alkaline Phosphatase ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Intraperitoneal Administration ◽

Chloroform Extract ◽

Hepatic Tissue ◽

Albino Rats ◽

High Dose ◽

Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

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Biological characterization of implant surfaces - in vitro study

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.1087 ◽

2015 ◽

Vol 44 (4) ◽

pp. 195-199 ◽

Cited By ~ 1

Author(s):

Priscilla Barbosa Ferreira Soares ◽

Camilla Christian Gomes Moura ◽

Huberth Alexandre da Rocha Júnior ◽

Paula Dechichi ◽

Darceny Zanetta-Barbosa

Keyword(s):

Alkaline Phosphatase ◽

Cell Viability ◽

Total Protein ◽

Cell Attachment ◽

Control Group ◽

Mitochondrial Activity ◽

Serum Total Protein ◽

Trypan Blue Exclusion ◽

Experimental Surface

<title>Abstract</title><sec><title>Objective</title>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</sec><sec><title>Material and method</title>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</sec><sec><title>Result</title>The values of cell viability were: 4h: C– 0.32±0.01A; Exp1– 0.34±0.08A; Exp2– 0.29±0.03A. 24h: C– 0.43±0.02A; Exp1– 0.39±0.01A; Exp2– 0.37±0.03A. The cell adhesion counting was: C– 85±10A; Exp1- 35±5B; Exp2– 20±2B. The amounts of serum total protein were 4d: C– 40±2B; Exp1– 120±10A; Exp2– 130±20A. 7d: C– 38±2B; Exp1– 75±4A; Exp2– 70±6A. 14 d: C– 100±3A; Exp1– 130±5A; Exp2– 137±9A. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1C; Exp1– 5.1±0.8B; Exp2– 9.8±2.0A. 7d: C– 1.0±0.01C; Exp1– 5.3±0.5A; Exp2– 3.0±0.3B. 14 d: C– 4.1±0.3A; Exp1– 4.4±0.8A; Exp2– 2.2±0.2B. Different letters related to statistical differences.</sec><sec><title>Conclusion</title>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</sec>

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Haematological and serum chemistry profiles of ferrets (Mustela putorius furo)

Laboratory Animals ◽

10.1258/002367782781110241 ◽

1982 ◽

Vol 16 (2) ◽

pp. 133-137 ◽

Cited By ~ 32

Author(s):

Elsbeth J. Lee ◽

W. E. Moore ◽

H. C. Fryer ◽

H. C. Minocha

Keyword(s):

Alkaline Phosphatase ◽

Weight Gain ◽

Total Protein ◽

Alanine Aminotransferase ◽

Inorganic Phosphate ◽

Sorbitol Dehydrogenase ◽

Serum Chemistry ◽

Mustela Putorius ◽

Mustela Putorius Furo

Female, male and castrated male ferrets were studied. Weight gain plateaued at 28-weeks of age with males about 500 g heavier than females. No statistically significant differences in haematology were observed with age, but alkaline phosphatase and alanine aminotransferase levels fell while glucose increased. Haemolysis led to various changes including marked increases in total protein, albumin, inorganic phosphate and sorbitol dehydrogenase.

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The effects of different anticoagulants on biochemical parameters in blood of dairy cows

Veterinarski glasnik ◽

10.2298/vetgl1502013b ◽

2015 ◽

Vol 69 (1-2) ◽

pp. 13-20

Author(s):

Branislava Belic ◽

Marko Cincovic ◽

Maja Dosenovic ◽

Dragica Stojanovic ◽

Zorana Kovacevic

Keyword(s):

Alkaline Phosphatase ◽

Dairy Cows ◽

Alkaline Phosphatase Activity ◽

Total Protein ◽

Biochemical Parameters ◽

Biochemical Analysis ◽

Blood Parameters ◽

Albumin Level ◽

Blood Samples ◽

Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

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