scholarly journals The application of a vascular closure device for closing a gastrostomy opening used for procedural access

2019 ◽  
Vol 92 (1099) ◽  
pp. 20180837
Author(s):  
Sung Bum Cho ◽  
Hyoung Rae Kim ◽  
Eui-Chul Jung ◽  
Hwan Hoon Chung ◽  
Seung Hwa Lee ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Closure Device ◽  
Leak Pressure ◽  
Vascular Closure Device ◽  
Significant Difference ◽  
Manual Suture ◽  
Survival Studies ◽  
Post Hoc ◽  
Experimental Group

Objective: To study the feasibility of applying the Perclose ProGlide vascular closure device (PPVCD) in vitro for closing a gastrostomy opening for procedural access in the swine stomach in order to prevent peritoneal leakage. Methods: The study included four experimental groups: one manual suture (n = 10), two manual sutures (n = 10), one PPVCD suture (n = 10), and two PPVCD sutures (n = 5). In the two PPVCD sutures group, the “pre-close” technique was used. The leak pressure was measured, and statistical analysis was conducted to compare the leak pressures among the experimental groups. Results: The gastrostomy openings were successfully closed in all experimental groups. The median (range) values of leak pressure (mmHg) for each experimental group were as follows: one manual suture, 86.0 (75.0–110.0); two manual sutures, 98.5 (44.0–130.0); one PPVCD suture, 96.5 (56.0–119.0); and two PPVCD sutures, 98.0 (66.0–104.0). The Mann–Whitney U test revealed no statistically significant difference in leak pressure between the manual (n = 20) and PPVCD (n = 15) suture groups. The Kruskal–Wallis test revealed no statistically significant difference in leak pressure among the four experimental groups. The Bonferroni post hoc test also revealed no statistically significant difference in the pairwise comparisons among the groups. Conclusion: Application of PPVCD is feasible for the closure of gastrostomy openings in an animal model and is as effective as a manual suture. Advances in knowledge: In our in vitro study, percutaneous closure of gastrostomy opening using PPVCD was possible; animal survival studies and development of specific devices are needed before clinical application.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals The Effect of Glaze Coating on Wear Resistance of Some Acrylic Denture Teeth: An In Vitro Study

2021 ◽  
Vol 13 (2) ◽  
pp. 47-51
Author(s):  
Samaneh Abbasi ◽  
Soodabe Ebrahimi ◽  
Arash Shisheian ◽  
Maryam Farhadian
Keyword(s):  
Wear Resistance ◽  
In Vitro Study ◽  
Resistance Rate ◽  
Vitro Study ◽  
Wear Rates ◽  
Denture Teeth ◽  
Significant Difference ◽  
The Mean ◽  
Post Hoc

Background: Acrylic resin teeth wear resistance has an important role in the denture longevity. This study aimed to clarify the effect of glaze coating on wear resistance of three types of artificial acrylic teeth. Methods: In this in vitro study, the wear resistance rate of three of acrylic denture teeth (GENIUS, STON and CLASSIC) was compared with Ivoclar teeth (n=25/group). The wear resistance was measured by estimating the weight loss in pre and post removing glaze coating, following 5000 cycles in the chewing simulator device. Data analysis was made using paired t test, one-way ANOVA and Tukey’s post hoc test. Results: ANOVA test showed that there was no significant difference between the mean amount of wear of GENIUS, CLASSIC, STON and IVOCLAR teeth in the first stage (P <0.061), but this difference was significant (P <0.001) in the second stage. The result of Tukey post hoc test showed that wear rates of GENIUS were significantly lower than other groups (P<0.001). Comparison between the mean wear rates of each dental group at the first and second stages showed a significant difference between average teeth wear resistance of CLASSIC, STON and IVOCLAR in the first and second stages (P <0.001). Conclusions: In conclusion, the teeth wear resistance of STON and CLASSIC were similar to IVOCLAR. Also, after removing the glaze coating, the teeth wear resistance decreased in all groups but was not statistically significant for group GENIUS.

scholarly journals Evaluation of Cyclic Fatigue of Hyflex EDM, Twisted Files, and ProTaper Gold Manufactured with Different Processes: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Pooja D. Khandagale ◽  
Prashant P. Shetty ◽  
Saleem D. Makandar ◽  
Pradeep A. Bapna ◽  
Mohmed Isaqali Karobari ◽  
...  
Keyword(s):  
Fatigue Resistance ◽  
Root Canal ◽  
File Systems ◽  
In Vitro Study ◽  
Cyclic Fatigue ◽  
Segment Length ◽  
Significant Difference ◽  
Number Of Cycles ◽  
Post Hoc

Introduction. The main aims of root canal instrumentation are to provide an environment that will lead to healing and to provide a root canal shape that is comfortable to clean and seal. When working with rotary endodontic instruments, the most significant concerns are that the instrument might fracture in the root canal, thus affecting the treatment outcome. Hence, it is of immense importance to know which file systems have more cyclic fatigue resistance. Methodology. This study evaluated the effect of the curved segment length of the artificial canal (the arch), and the number of cycles necessary in fracture of Hyflex EDM, Twisted files, and ProTaper Gold were recorded. Sixty NiTi rotary instruments of 25 mm length (Hyflex EDM (20), Twisted files (20), and ProTaper Gold (20)) were tested in a metal block with simulated canal having 90° angle of curvature. The study was performed with a specific radius and degree of curvature, i.e., 8 mm radius and 90⁰ angle of curvature, and data obtained were subsequently subjected to statistical evaluation using one-way analysis of variance and Tukey’s post hoc test. Result. The Hyflex EDM (774.29) exhibited the maximum cyclic fatigue resistance compared to Twisted files (654.875) and ProTaper Gold (375.575). A statistically significant difference was observed between the tested groups. Conclusion. The Hyflex EDM files showed the highest cyclic fatigue resistance, followed by Twisted files and ProTaper Gold files.

scholarly journals Estimation of Total Fluoride Concentration, Total Soluble Fluoride Concentration and pH among Various Brands of Toothpastes

2021 ◽  
Author(s):  
Praneetha D Rani ◽  
Vijaya Hegde
Keyword(s):  
Group Analysis ◽  
Fluoride Concentration ◽  
In Vitro Study ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Institute Of Technology ◽  
Level Of Significance ◽  
Post Hoc

Introduction: Fluoride toothpastes are a major reason for the decline in dental caries globally. For fluoride toothpaste to be effective, an adequate amount of Total Soluble Fluoride (TSF) must be available in the toothpastes. Aim: To determine and compare the Total Fluoride (TF), TSF and pH among various toothpaste brands. Materials and Methods: An in-vitro study was conducted during October 2019 on 20 toothpaste samples, which belonged to four groups namely, herbal toothpastes group, nonherbal toothpastes group, medicated toothpastes group and kids toothpastes group. Analysis of the samples was done at the Department of Environmental Engineering Laboratory, National Institute of Technology, Surathkal, Karnataka, India. The TF and the TSF concentration was determined using fluoride ion electrode. The pH was determined using pH meter. Statistical analysis was performed using Statistical Package for Social Sciences (SPSS version 19.0). Analysis of variance (ANOVA) was used by Tukey’s post-hoc test to determine the difference between groups. The level of significance was kept at 0.05. Results: The results showed that there was a significant difference between the toothpaste types for mean TF concentration (p=0.004). The post-hoc test showed that there was a significant difference between herbal and nonherbal toothpastes (p=0.041) where the mean TF concentration for nonherbal toothpastes was 1095.20 ppm when compared to herbal toothpastes was 704.40 ppm. The results showed that there was a siginificant difference between the toothpaste types for Mean TSF concentration (p<0.003). There was a significant difference between medicated and kids toothpastes (p=0.024) where the mean total soluble fluoride concentration for medicated toothpastes was 938.60 ppm when compared to the kids toothpastes was 521.20 ppm. Conclusion: The present study showed that pH of all the toothpastes were either neutral or alkaline and the TSF concentration was less when compared to TF concentrations.

scholarly journals Comparative Evaluation of Different Irrigation Techniques with Conventional Irrigation Technique for the Removal of Double Antibiotic Paste from Root Canal-An in vitro study

2020 ◽  
Vol 11 (4) ◽  
pp. 5578-5583
Author(s):  
Samrudhi Khatod ◽  
Anuja Ikhar ◽  
Pradnya Nikhade ◽  
Manoj Chandak ◽  
Kaustubh Khatod ◽  
...  
Keyword(s):  
Root Canal ◽  
Irrigation System ◽  
In Vitro Study ◽  
Complete Removal ◽  
Passive Ultrasonic Irrigation ◽  
Significant Difference ◽  
Ultrasonic Irrigation ◽  
Double Antibiotic Paste ◽  
Experimental Group

Complete removal of infection, rendering root canal free of an organism is the prime aim of endodontic treatment. It cannot be achieved alone by biomechanical preparation due to the complexity of root canal treatment. Therefore the use of intracanal medicament is a must. The antibiotic paste is frequently used intracanal medicament, usually in regenerative cases. Hence the aim of the is to compare different irrigation technique for removal of double antibiotic paste from root canal through a stereomicroscope. 36 single-rooted were standardized to root length of 12mm then instrumented by Protaper rotary file up to size F4. Irrigation was done using sodium hypochlorite in between instrument change. The root canal was dried using paper point and filled by DAP and then randomly allocated to 3 groups(n=12) according to irrigation system used: conventional syringe irrigation (CSI), Passive ultrasonic irrigation(PUI), EndoVac (EV). Roots were then longitudinally sectioned using the diamond disk and studied under a stereomicroscope using a scoring scale. Data were evaluated using one way ANOVA and Tukey test. Among all experimental group CSI was least efficient. PUI and EV showed greater efficiency but no significant difference between PUI and EV, but a significant difference between CSI and PUI, EV (p&lt; 0.05). Use of irrigation activation system results in efficient removal of DAP compared to CSI.

scholarly journals Studying the Optical 3D Accuracy of Intraoral Scans: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Pokpong Amornvit ◽  
Sasiwimol Sanohkan ◽  
Chaimongkon Peampring
Keyword(s):  
High Resolution ◽  
Computer Software ◽  
In Vitro Study ◽  
Reference Points ◽  
Washington Dc ◽  
Scanning Area ◽  
Significant Difference ◽  
Post Hoc ◽  
Digital Caliper

There are various scanners available in dental practice with various accuracies. The aim of this study was to compare the 3D capturing accuracy of scans obtained from Trios 3 and Dental Wings scanner. A reference mandibular model was printed from FormLab with reference points in three axes (X, Y, and XY and Z). The printed model was scanned 5 times with 3 scans: normal scan by Trios 3 (Trios 3A), high-resolution scan by Trios 3 (Trios 3B), and normal scan by Dental Wings. After scan, the stereolithography (stl) files were generated. Then, the measurements were made from the computer software using Rhinoceros 3D (Rhino, Robert McNeel & Associates for Windows, Washington DC, USA). The measurements made with digital caliper were taken as control. Statistical analysis was done using one-way ANOVA with post hoc using Sheffe (P<0.01). Trios 3 presented higher accuracy than Dental Wings and high resolution showed better results. The Dental Wings showed less accuracy at the measurements >50 mm of length and >30 mm in width. There was no significant difference (P>0.05) of control with the Trios 3A and Trios 3B. Similarly, for the measurements in Z-axis, there was no significant difference of control with each scan (Trios 3A, Trios 3B, and Dental Wings). Accuracy of the scan is affected by the length of the scanning area and scanning pattern. It is less recommended to Dental Wings scan >3-unit prosthesis and that crosses the midline.

scholarly journals Shaping ability of two heat treated rotary NiTi instruments using different kinematics/in vitro study

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Engy M. Kataia ◽  
Mohamed Mokhtar Nagy ◽  
Mohamed M. Kataia ◽  
Hala Fayek Khalil
Keyword(s):  
In Vitro Study ◽  
Preparation Time ◽  
Heat Treated ◽  
Significant Difference ◽  
Before And After ◽  
Canal Transportation ◽  
Niti Instruments ◽  
Post Hoc ◽  
Shaping Ability

Abstract Background Comparing shaping ability of Azure and Fanta AF One in rotation and reciprocation. This study was conducted on 40 resin blocks, having canal curvatures 30°-angle and of 16-mm length. The blocks were assigned to 4-groups according to the instrument and motion used: Azure/Rotation (A/Rt), Fanta AF one/Rotation (F/Rt), Azure/Reciprocation (A/Rec) and Fanta AF one/Reciprocation (F/Rec). For both systems single file 25, taper 0.06 were used. Digital images were taken before and after instrumentation. Adobe Photoshop was used to overlap post and pre-instrumentation images to assess canal transportation at 1, 4, 7-mm; representing apical, middle and cervical levels respectively. Stopwatch was used to document the time taken for canal preparation. One-way ANOVA followed by Tukey post hoc test, used to compare between more than two groups in non-related samples. Results At the apical level, no statistically significant difference was found between both files in either rotation or reciprocation. However, reciprocation motion showed significantly lower transportation levels in both files. No significant difference was found among groups at the middle and cervical levels. Regarding preparation time, Reciprocation in both groups was significantly longer. Conclusions The use of reciprocation motion showed minimized apical canal transportation in both files however in longer preparation time.

scholarly journals Comparative evaluation of anti-bacterial efficacy of silver nanoparticle, nanoparticle calcium hydroxide and calcium hydroxide against enterococcus faecalis biofilm- An In vitro study

2018 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Dr.Abirami V. ◽  
Dr.A.Shafie Ahamed ◽  
Dr.Bhavani S. ◽  
Dr.Rajaraman G.
Keyword(s):  
Silver Nanoparticles ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Silver Nanoparticle ◽  
In Vitro Study ◽  
P Value ◽  
Antibacterial Efficacy ◽  
Significant Difference ◽  
Post Hoc

The purpose of this study was to evaluate the antibacterial efficacy of silver Nanoparticles, Nanoparticle calcium hydroxide and calcium hydroxide as intracanal medicament against Enterococcus faecalis bio film formed on root dentinThe Minimal inhibitory concentration test and Anti microbial test were conducted for silver nanoparticle, Nanoparticle calcium hydroxide. Teeth were inoculated with E.faecalis strains for 1month to establish a standard mono species bio film model. Biofilm formation is confirmed using SEM. The intracanal medicaments were placed inside the canal and colony forming units (CFU) were counted before and 7 days later the placement of the medicament.MIC for AGNP is 25µg/200µl and for NCH is 12.5µg/200µl. The Antimicrobial test conducted shows zone of inhibition about 11mm and 14mm for AGNP and NCH respectively at 500µg. The calcium hydroxides do not show any antibacterial activity at this concentration. In antibacterial efficacy test using CFU count, the AGNP and NCH shows 75% and 61% of antibacterial efficacy respectively whereas calcium hydroxide has exhibited just 11% of antibacterial efficacy. Silver Nanoparticles and nanoparticle calcium hydroxide had statistically significant difference in the reduction in the number of colonies when compared to calcium hydroxide by post hoc turkey test and p-value of <0.01 was considered as significant in this study.

scholarly journals The Evaluation of apically extruded debris during root canal preparation using Pro-Taper Universal, ProTaper Gold and Reciproc Blue system: An in-vitro comparative Study

2020 ◽  
Vol 6 (2) ◽  
pp. 11-15
Author(s):  
Padma Chandra ◽  
Rajesh Prasad ◽  
Surendra Kumar Mishra ◽  
Jagdish Chandra
Keyword(s):  
File System ◽  
In Vitro Study ◽  
Nickel Titanium ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significance Level ◽  
Significant Difference ◽  
Bonferroni Test ◽  
Post Hoc

ABSTRACT Aims & Objectives: The purpose of this study was to compare the amount of apically extruded debris with four nickel-titanium instruments i.e ProTaper Universal, ProTaper Gold, and Reciproc Blue system, Hand K file System. Methods: Eighty extracted single-rooted mandibular premolar human teeth were randomly assigned to 4 groups (n = 20). The canals were instrumented using 1 of the following instruments: ProTaper Universal, ProTaper Gold, and Reciproc Blue system, Hand K file System. Apically extruded debris during instrumentation was collected into preweighed Eppendorf tubes. The weight of the extruded debris was calculated by subtracting the weight of the empty tubes from that of the tubes containing the debris. The data were analyzed statistically using the analysis of variance (ANOVA) and Post-hoc bonferroni test at a significance level of P < .05. Results: All the instrumentation systems resulted in production of extruded debris. The amount of extruded debris was significantly more in Hand K file than PTU, PTG and Reciproc Blue (P<0.05). The PTU system was associated with significantly more extruded debris than the PTG(P=0.041), Reciproc Blue systems (P=`0.011), whereas no significant difference was found in the amount of the debris extruded between PTG and Reciproc Blue (P=0.271). The amount of extruded debris was least in Reciproc Blue in comparison to all used system. Conclusions: Within the limitations of this in vitro study, the amount of apically extruded debris registered for the different files tested was highest for hand file, Protaper Universal, followed by the ProTaper Gold and least in Reciproc Blue.

scholarly journals COMPARATIVE EVALUATION OF IATROGENIC DAMAGE IN CLASS II CAVITIES USING MAGNIFICATION TOOLS DURING TOOTH PREPARATION: AN IN VITRO STUDY

2020 ◽  
Vol 8 (11) ◽  
pp. 569-575
Author(s):  
Pooja Yadav ◽  
◽  
Ashish Jain ◽  
Rahul Rao ◽  
Harshil Patel ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Class Ii ◽  
Vitro Study ◽  
Tooth Surface ◽  
Naked Eye ◽  
Significant Difference ◽  
Iatrogenic Damage ◽  
Tooth Preparation ◽  
Post Hoc

Aims: This is an vitro study compares and evaluates the effect of magnification tools on frequency and extent of iatrogenic damage to approximal tooth surface during conservative Class II cavity preparations. Methods and Material: 30 Typodont teeth were divided into 3 groups 10 typodont teeth in each group and were mounted on Phantom head. Teeth were prepared for class II (MO) cavity on 36 with conservative design using airotor with naked eye, loupes and microscope and iatrogenic damage was assessed on tooth no 35. Assessment of iatrogenic damage of all the groups was done by profilometer test. Statistical analysis used:Statistical analysis was done using one-way analysis of variance (ANOVA). Post-hoc pair-wise comparisons were done using Dunnetts test. Results: Results revealed that tooth preparation was better under microscopes and loupes with statistical significant difference for samples with loupes and microscope on comparison with naked eye. Study also expressed the difficulty faced during tooth preparation with microscope and loupes for the first time. Conclusions: Magnifying tools helps in better vision and less iatrogenic damage while preparing the tooth for restoration.

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