scholarly journals Effects of ruminal pH and subacute ruminal acidosis on milk yield and composition of Holstein cows in different stages of lactation

2019 ◽  
Vol 70 (2) ◽  
pp. 1551
Author(s):  
G. C. KITKAS ◽  
G. E. VALERGAKIS ◽  
M. KRITSEPI-KONSTANTINOU ◽  
A. I. GELASAKIS ◽  
G. ARSENOS ◽  
...  

Subacute ruminal acidosis (SARA; implying a rumen fluid pH between 5.5 and 5.0), is one of the most important metabolic diseases of dairy cows. In this study, the effect of SARA and rumen fluid pH on milk yield and composition was assessed in dairy cows under field conditions, with repeated measurements in the same cows, at different stages of lactation. Rumenocentesis was performed in 83 Holstein cows of a commercial herd at 30, 90, and 150 days in milk (DIM). Rumen fluid pH was measured on-site using a portable pH-meter. Milk yield was also recorded at the same days. Milk samples were analyzed for fat, protein, lactose and total solids content. For the statistical analysis, mixed linear regression models were used. Prevalence of SARA was 48.2%, 53.8% and 65.3% at 30, 90 and 150 DIM, respectively. There was a significant negative effect of SARA and decreased rumen fluid pH on milk fat content; SARA was associated with a decrease of milk fat content by 0.22%, while a one-unit increase of rumen fluid pH, even within the normal range, was associated with a 0.28% increase of milk fat content and 0.44% increase of milk total solids content. There was no effect of SARA on milk yield or protein, lactose and total solids content. In conclusion, under field conditions, SARA and decreased rumen fluid pH reduce milk fat content.

Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


2012 ◽  
Vol 92 (3) ◽  
pp. 353-364 ◽  
Author(s):  
S. Li ◽  
G. N. Gozho ◽  
N. Gakhar ◽  
E. Khafipour ◽  
D. O. Krause ◽  
...  

Li, S., Gozho, G. N., Gakhar, N., Khafipour, E., Krause, D. O. and Plaizier, J. C. 2012. Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows. Can J. Anim. Sci. 92: 353–364. Effects of subacute ruminal acidosis (SARA) challenges on measurements of feces, urine, milk and blood samples, and of feeding behavior were investigated to determine which of these measurements may aid in the diagnosis of SARA. Eight multiparous lactating dairy cows were used in a crossover design with two 6-wk experimental periods. During weeks 1, 2, and 6, cows received a control diet with a forage-to-concentrate ratio of 58:42. During weeks 3 to wk 5, a grain-based SARA challenge (GBSC) or an alfalfa-pellet SARA challenge (APSC) was conducted by replacing 12% of the dry matter of the control ration with pellets containing 50% ground wheat and 50% ground barley, and by replacing 26% of the dry matter of the control ration with pellets of ground alfalfa, respectively. The rumen pH depression did not differ between the challenges. The GBSC increased the concentrations of lipopolysaccharide (LPS) in feces and of serum amyloid A in blood, but decreased that of milk fat and urea in blood. The APSC increased the urine pH, the net-acid-base excretion, and the red blood cell count and potassium concentration in blood. Both challenges increased the concentrations of LPS and propionate in rumen fluid, protein in milk, glucose, lactate and sodium and the partial pressure of CO2in blood, and tended to decrease the concentration of chloride in blood. The measures that were similarly affected by both challenges may aid in the diagnosis of a rumen pH depression. Differences between the SARA challenges suggest that this disorder is not solely rumen pH dependent.


1990 ◽  
Vol 70 (2) ◽  
pp. 731-734 ◽  
Author(s):  
A. S. ATWAL ◽  
J. D. ERFLE

Large day-to-day variations in milk fat, particularly for the morning milkings, were observed in 36 Holstein cows. Changes in percent fat were gradual and produced wavelike patterns in a number of instances. Supplemental feeding of long hay had no effect on acetate/propionate ratio in rumen fluid, daily milk yield or weighted milk fat percentage. Key words: Dairy cows, milk, fat depression, hay


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2401
Author(s):  
Xiaoge Sun ◽  
Yue Wang ◽  
Erdan Wang ◽  
Shu Zhang ◽  
Qianqian Wang ◽  
...  

High-yield dairy cows with high-concentrate diets are more prone to experiencing health problems associated with rumen microbial imbalance. This study assessed the effects of Saccharomyces cerevisiae culture (SC), a food supplement, on ruminal pH, volatile fatty acid (VFA), inflammatory cytokines, and performance of high-yield dairy cows. Forty Holstein cows with similar characteristics (e.g., milk yield, days of milk, and parity) were randomly divided into two groups: an experimental group fed the basal ration supplemented with the SC of 100 g of SC per cow per day (hour, SC group), and a control group fed the same basal ration diet without SC (i.e., CON group). On average, the supplementation of SC started at 73 days of lactation. The experimental period lasted approximately 70 days (from 18 January to 27 March 2020), including 10 days for dietary adaptation. Milk yield was recorded daily. Rumen fluid and milk samples were collected after 2 h of feeding in the morning of day 0, 15, 30, and 60. The data showed that rumen pH increased (p < 0.05) when cows were provided with SC. On average, the cows in the SC group produced 1.36 kg (p < 0.05) more milk per day than those in the CON group. Milk fat content of cows in the SC group was also higher (4.11% vs. 3.96%) (p < 0.05). Compared with the CON group, the concentration of acetic acid in the rumen fluid of dairy cows in the SC group was significantly higher (p < 0.05). There were no differences (p > 0.05) found in milk protein content and propionic acid between groups. The SC group had a tendency increase in butyric acid (p = 0.062) and total VFA (p = 0.058). The result showed that SC supplementation also enhanced the ratio between acetic and propionic. Most of the mean inflammatory cytokine (IL-2, IL-6, γ-IFN, and TNF-α) concentrations (p < 0.05) of the SC group were lower than CON group. This study demonstrated that high-yield cows receiving supplemental SC could produce more milk with higher fat content, have higher rumen acetate, and potentially less inflammatory cytokines.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 179-180
Author(s):  
Xiaoge Sun ◽  
Shu Zhang ◽  
Erdan Wang ◽  
Na Lu ◽  
Wei Wang ◽  
...  

Abstract Dramatic increases in milk yields in recent decades have created challenges in terms of rumen pH and microbial health which ultimately impact dairy cow health. The objective of this study was to assess the effects on ruminal pH, Volatile Fatty Acid (VFA), microbiota, inflammation, and performance of high-yield dairy cows by supplementing Saccharomyces cerevisiae culture (SC). Forty Holstein cows were divided into two groups based on their milk yield, days of milk, and parity fed the same basal ration diet that did or did not contain 100 g of SC /cow per day. Individual dry matter intake (DMI) and milk yield were recorded each day. Rumen fluid and milk samples were collected after 2 hours of morning feeding at intervals of 15 days during the experiment period. The data showed that rumen pH was increased by 0.19 (P = 0.09) when SC was supplemented than no SC was provided. SC-supplemented cow consumed 0.28 kg (P &lt; 0.05) extra DM/d. Those supplemented with SC produced 1.36 kg (P &lt; 0.05) more milk/cow per day than did non-supplemented cows. Milk fat percentage was higher (4.11 vs. 3.96%) for cows receiving SC. There were no differences in milk protein percentage. Rumen fluid VFA concentration was not statistically affected by SC but was numerically higher acetic and lower propionic for supplemented cows. The blood of the SC group with lower inflammation cytokines and somatic cell count (SCC). SC-supplemented cows had a greater relative abundance of Prevotellaceae, Succinivibrionaceae, Fibrobacteraceae, Lactobacillaceae, and lower relative abundance of Spirochaetaceae, Methanobacteriaceae, Enterobacteriaceae than the unsupplemented cows. It had greater functions on xylanolysis, fermentation, cellulolysis in the rumen in terms of the KEGG function prediction analysis. This study demonstrated that high-yield lactation cows receiving supplemental SC produced more milk and potentially reduced the inflammation and enhanced rumen cellulolysis bacteria growth.


2021 ◽  
Vol 19 (3) ◽  
pp. 311
Author(s):  
Inggit Kentjonowaty ◽  
Achmad Bagus Adhiluhung Mardhotillah ◽  
Trinil Susilawati ◽  
Puguh Surjowardojo

<p class="MDPI17abstract"><strong>Objective: </strong><span lang="EN-GB">The objective of this study was conducted to evaluate the effects of Mammae Hand Massages (MHM) on oxytocin release, milk yield, and milk quality in dairy cows.</span></p><p class="MDPI17abstract"><strong>Methods: </strong><span lang="EN">Twelve dairy cows with the following criterion: 5-yr-old, 1st to 9th month of lactation, and average body weight of 390 ± 5.55 kg were used. Cows were assigned in a randomized block design with 4 treatments, i.e T0 (without massage), T1 (MHM for 20 s), T2 (MHM for 50 s), and T3 (MHM for 80 s). Oxytocin release, milk yield, and milk quality were measured accordingly</span><span lang="EN-GB">.</span><strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong><span lang="EN">The data obtained were analyzed using analysis of variance (ANOVA). The results showed that MHM had a very significant effect on milk production (P &lt;0.01) as evidenced by the MHM 50 s treatment obtained the highest average milk production (13.08 ± 3.38 liters/head/day) compared to the MHM 20 s, MHM 80 s and control. MHM (1-1.6 volts) for 50 s resulted in the highest release of oxytocin (0.22955 pcg/0.1mL) at 240 seconds compared to control, MHM 20 s and 80 s. Likewise, the percentage of milk protein content (2.96 ± 0.03) and milk fat content (4.27 ± 0.70) was highest at MHM 50 s</span><span lang="EN-GB">.</span><strong></strong></p><strong><span>Conclusions: </span></strong><span lang="EN">It can be concluded that MHM (1-1.6 volts) for 50 seconds increases the release of oxytocin, milk production, and milk quality in terms of the percentage of milk protein and fat content</span><span lang="EN-GB">.</span>


2013 ◽  
Vol 16 (4) ◽  
pp. 813-821
Author(s):  
A.M. Brzozowska ◽  
K. Słoniewski ◽  
J. Oprządek ◽  
P. Sobiech ◽  
Z.M. Kowalski

Abstract One of the largest challenges for the dairy industry is to provide cows with a diet which is highly energetic but does not negatively affect their rumens’ functions. In highly productive dairy cows, feeding diets rich in readily fermentable carbohydrates provides energy precursors needed for maximum milk production, but simultaneously decreases ruminal pH, leading to a widespread prevalence of subacute ruminal acidosis. Maximizing milk production without triggering rumen acidosis still challenges dairy farmers, who try to prevent prolonged bouts of low ruminal pH mainly by proper nutrition and management practices. The animals try to avoid overeating fermentable feeds, as it causes negative consequences by disturbing digestive processes. The results of several experiments show that ruminants, including sheep and beef cattle, are able to modify some aspects of feeding behaviour in order to adjust nutrient intake to their needs and simultaneously prevent physiological disturbances. Particularly, such changes (e.g., increased preference for fibrous feeds, reduced intake of concentrates) were observed in animals, which were trying to prevent the excessive drop of rumen fluid pH. Thanks to a specific mechanism called “the postingestive feedback”, animals should be able to work out such a balance in intake, so they do not suffer either from hunger or from negative effects of over-ingesting the fermentable carbohydrates. This way, an acidosis should not be a frequent problem in ruminants. However, prolonged periods of excessively decreased rumen pH are still a concern in dairy cows. It raises a question, why the regulation of feed intake by postingestive feedback does not help to maintain stable rumen environment in dairy cows?


2012 ◽  
Vol 172 (3-4) ◽  
pp. 141-149 ◽  
Author(s):  
S. Iqbal ◽  
S.J. Terrill ◽  
Q. Zebeli ◽  
A. Mazzolari ◽  
S.M. Dunn ◽  
...  

2018 ◽  
Vol 51 ◽  
pp. 131-139 ◽  
Author(s):  
Y. V. Poslavska ◽  
E. I. Fedorovych ◽  
P. V. Bodnar

Selection and breeding work, which is responsible for realization and further improvement of genetic potential of animals, has a significant role in solving problems of industry competitiveness of dairy sector. Increase of animal productivity due to better development of those body parts which impact on milk productivity directly or indirectly, and elimination of some defects in appearance, affecting on various economic useful traits of animals may be one of the breeding work directions. Considering the above mentioned, the aim of our research was to study dependence of milk productivity of Ukrainian Black-and-White Dairy cows on their body measurements during the period of their first lactation. The research was conducted at Ukrainian Black-and-White Dairy cows in "Milk rivers" LLC of Sokal district, Lviv region. Exterior of first-сalf heifers (measurements of withers height, the depth of the chest, chest width, chest girth for scapula, oblique body length (by stick), width in hips, girth of metacarpus) and evaluation of dairy cow productivity during the first, second, third and better lactation (milk yield, fat content in milk, the amount of milk fat) were carried out according to the data of zootechnical accounting (during last 30 years). It was revealed that the experimental first-calf heifers had proportional development of a body, a deep and voluminous chest. Withers height was 131,0, chest depth – 73,1, chest width – 45,2, chest girth for scapula – 192,7, oblique body length – 156,3, width in hips – 51,8 and girth of metacarpus – 18,6 cm. It should be noted that the animals on most of the evaluated measurements, slightly, but prevailed the target parameters for the desired type of Ukrainian Black-and-White dairy cattle (exception – chest width). Narrow chests of the first-calf heifers were inherited from Holstein bull-sires, which for the recent decades, have widely been used in domestic herds of cattle. The variability level of the investigated measurements of a body was low; it testifies the consolidation of the herd. According to the measurements it was within 3,11–9,79 %. It should be noted that measurements of chest width (9,79 %) and width in hips (8,11 %) were observed with the highest variability. The milk yields of the Ukrainian Black-and-White Dairy cows of the herd were 3728,2 kg during the first lactation, 3936,8 kg during the second, 4375,2 kg during the third and 4446,3 kg during the best, fat content in milk – 3,86; 3,80; 3,78 and 3,82 % respectively and the amount of milk fat – 143,8; 149,7; 165,5 and 169,8 kg. As a result of the research we found the dependence of milk productivity of the cows on their body measurements during the first lactation. The highest milk yield and the amount of milk fat were characterized for animals, which withers height was 130–132, the depth of the chest – 74–76, the width of the chest – 43–45, chest girth – 195–199, oblique body length – 155–159, width in hips – 55–57, girth of metacarpus – 18,1–19,0 cm during the first lactation. According to the abovementioned indicators of milk productivity, they exceeded animals of all other experimental groups, but this predominance was significant only in some cases. High significant relationships between exterior measurements of the first-calf heifers and their further milk production were revealed. The correlation coefficients between body measurements of first-calf heifers and milk yields, depending on measurement and lactation, were within 0,100-0,388, between body measurements and fat content in milk – within 0,145–0,347 and between body measurements and the amount of milk fat – within 0,132–0,388, and share of impact of measurements on these indicators of milk productivity – within 8,68–36,38; 10,80–28,42 and 8,83–34,96 % respectively.


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