scholarly journals Characterization and stability evaluation of nanoencapsulated epoxylignans

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 253
Author(s):  
Yusnita Rifai ◽  
Radhia Riski ◽  
Gemini Alam ◽  
Magdalena Litaay ◽  
Latifah Rahman
Keyword(s):  
Physical Stability ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Stability Evaluation ◽  
Therapeutic Application ◽  
Polymer Stabilizer ◽  
Diffraction Patterns ◽  
Eudragit Rl ◽  
Main Strategy ◽  
Potential Antitumor

3',6-dimethoxy-3'',4''-(methylenedioxy)-2,5-epoxylignan-4'-ol (DMEO), an epoxylignan isolated from Piper nigrum, has currently captured attention for its potential antitumor effect. However, low stability is limiting its therapeutic application. The application of nanocapsulation would be the main strategy for overcoming this problem. DMEO-loaded nanocapsules were prepared by an emulsion-diffusion method using Eudragit RL 100 (at concentrations of 1, 1.5 and 2%) and polyvinyl alcohol. As the polymer content increased, the encapsulation efficiency and mean particle size also increased. After 6 months of storage at 25°C (0% RH), no crystalline peaks were observed in the diffraction patterns of all nanocapsules, thereby suggested that the physical stability of nanoencapsulated DMEO was not affected by the concentration ratio of the polymer−stabilizer combinations.

scholarly journals Characterization and stability evaluation of nanoencapsulated epoxylignans

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 253
Author(s):  
Yusnita Rifai ◽  
Radhia Riski ◽  
Gemini Alam ◽  
Magdalena Litaay ◽  
Latifah Rahman
Keyword(s):  
Physical Stability ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Stability Evaluation ◽  
Therapeutic Application ◽  
Polymer Stabilizer ◽  
Diffraction Patterns ◽  
Eudragit Rl ◽  
Main Strategy ◽  
Potential Antitumor

3',6-dimethoxy-3'',4''-(methylenedioxy)-2,5-epoxylignan-4'-ol (DMEO), an epoxylignan isolated from Piper nigrum, has currently captured attention for its potential antitumor effect. However, low stability is limiting its therapeutic application. The application of nanocapsulation would be the main strategy for overcoming this problem. DMEO-loaded nanocapsules were prepared by an emulsion-diffusion method using Eudragit RL 100 (at concentrations of 1, 1.5 and 2%) and polyvinyl alcohol. As the polymer content increased, the encapsulation efficiency and mean particle size also increased. After 6 months of storage at 25°C (0% RH), no crystalline peaks were observed in the diffraction patterns of all nanocapsules, thereby suggested that the physical stability of nanoencapsulated DMEO was not affected by the concentration ratio of the polymer−stabilizer combinations.

scholarly journals Investigation Utilizing the HLB Concept for the Development of Moisturizing Cream and Lotion: In-Vitro Characterization and Stability Evaluation

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 43 ◽  
Author(s):  
Shoaib Alam ◽  
Mohammed S. Algahtani ◽  
Mohammad Zaki Ahmad ◽  
Javed Ahmad
Keyword(s):  
Physical Stability ◽  
Storage Conditions ◽  
Phase System ◽  
Stability Evaluation ◽  
Stable Emulsion ◽  
Promising Strategy ◽  
In Vitro Characterization ◽  
Hydrophilic Lipophilic Balance ◽  
Droplet Morphology

The current study aims to utilize the concept of the hydrophilic–lipophilic balance (HLB) value of ingredients for the development of a stable emulsion-based moisturizing cream and lotion for cosmetic application. The combination of a hydrophilic and lipophilic emulsifier such as glyceryl stearate (HLB value 3.8) and PEG-100 stearate (HLB value 18.8) were found to be effective to emulsify the chosen oil phase system at a specific concentration to achieve the required HLB for the development of the stable emulsion-based system. The developed formulation was characterized for pH, viscosity, spreadability, rheology, and droplet morphology. The influence of carbopol® ETD 2020 and the concentration of the oil phase on the rheology of the product was investigated and found to be significant to achieve the required thickening to convert the lotion into a cream. The formulation system developed through utilizing the concept of HLB was compared to a product developed through the conventional approach. It was observed that the utilization of the HLB method for the development of an emulsion-based product is a promising strategy compared to the conventional method. The physical stability and thermodynamic stability tests were carried out under different storage conditions. It was observed that the developed formulation was able to retain its integrity without showing any signs of instability during storage.

Antibacterial Activity of Alimentary Plants Against Staphylococcus aureus Growth

1994 ◽  
Vol 22 (02) ◽  
pp. 169-174 ◽  
Author(s):  
Cristina Pérez ◽  
Claudia Anesini
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Theobroma Cacao ◽  
Plant Material ◽  
Penicillin G ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Ripe Fruit ◽  
Ethanol Extracts ◽  
Agar Well Diffusion Method

Alimentary plants were screened for antibacterial activity against a penicillin G resistant strain of Staphylococcus aureus. Twenty-five samples of plant material corresponding to 21 species from 13 families were used. Both aqueous and ethanol extracts were obtained from them. Antibacterial activity was determined by the agar-well diffusion method, using cephazolin as a standard antibiotic. Seventeen ethanol extracts were found active. Eugenia caryophyllata (clavo de olor*) flowers, Myristica fragans (nuez moscada*) seeds, Theobroma cacao (cacao*) seed bark, Triticum sp (trigo*) fruit, Zea mays (maíz*) fruit and Piper nigrum (pimienta*) ripe fruit produced some of the more active extracts (*= Argentine vulgar names).

scholarly journals Antimitotic activity of Piper nigrum on clinical isolates of candida

2019 ◽  
Vol 10 (2) ◽  
pp. 1167-1171
Author(s):  
Gayathri Karthikeyan ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Ethanolic Extract ◽  
Fungal Species ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Disc Diffusion ◽  
Antimitotic Activity ◽  
Diffusion Technique

The objective of this study is to analyse the antimycotic activity of pepper on the clinical isolates of Candida. The extracts were prepared in the following concentrations in sterile water. 5mg/ml and 10mg/ml and 20mg/ml. 100µl of an extract of different concentrations were loaded on sterile filter paper discs measuring 6mm in diameter, so that the concentration of the extract on each disc was 500µg, 1000 µg and 2000µg respectively. The discs were dried and kept aseptically. Screening of antifungal activity [dis diffusion technique] The ethanolic extract of Piper nigrum was screened for antifungal activity by the disc diffusion method. Effect of three different concentrations (2000, 1000, 500,µg /disc) of the ethanolic extract Of Piper nigrum was tested against Candida albicans using disc diffusion technique. All the concentrations of the test solution inhibited the fungal species with varying degree of sensitivity. The inhibitory zone was measured with respect to the different concentration of the extracts which include 2000 µg/disc and 500 µg/disc at both room temperature and cold temperature (4-8°c). The inhibition zones were evaluated at 24 and 48 hrs. The result of this study revealed that the pepper oil has pronounced inhibitory activities against Candida albicans. This result is comparable with studies which have shown that Piper nigrum has a broad antibacterial activity. Since Piper nigrum is easily available and well-tolerated, it can be incorporated into medication for topical antifungal therapy as well.

scholarly journals Formulasi dan Evaluasi Sediaan Obat Kumur (Mouthwash) dari Ekstrak Etanol Daun Kalangkala (Litsea angulata) sebagai Antiseptik Mulut

2021 ◽  
Vol 7 (1) ◽  
pp. 248-256
Author(s):  
Rohama Rohama ◽  
Melviani Melviani
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Physical Stability ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Physical Quality ◽  
Stability Properties ◽  
Ideal Formula ◽  
The Ideal

Research of mouthwash formulation and evaluation of kalangkala leaves extract (Litsea angulata) as an oral antiseptic also has been tested in Streptococcus mutans bacteria by variation in the concentration of extract 2%, 2,5%, and 3%, which aims to determine the ideal formula in physical quality and has the highest antibacterial activity based on an inhibition zone of Streptococcus mutans bacteria in mouthwash formula. The methods used include the step of extraction of Kalangkala leaves, preparation of mouthwash with 3 formula F1 (2%), F2 (2,5%), and F3 (3%) followed by an evaluation that includes organoleptic, pH test, viscosity test, and the test of inhibition zone bacteria. The test of inhibition zone bacteria used NA media with diffusion method. Stability was performed on weeks 1, 2, 3, and 4. The result showed variation in the concentration of extract kalangkala leaves in a mouthwash formula affects the diameter of the inhibition zone. But did not have a significant effect on the physical stability properties of the mouthwash formula. Mouthwash formula has the highest antibacterial activity based on the inhibition zone of Streptococcus mutans that is 3% contained in formula F3.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oils from Black Pepper, Cumin, Coriander and Cardamom Against Some Pathogenic Microorganisms

2016 ◽  
Vol 20 (2) ◽  
pp. 39-52 ◽  
Author(s):  
Desislava Teneva ◽  
Zapryana Denkova ◽  
Bogdan Goranov ◽  
Rositsa Denkova ◽  
Georgi Kostov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Black Pepper ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Oil Composition ◽  
Gram Positive Bacteria ◽  
Inhibition Zones ◽  
Main Components

Abstract Four popular spices black pepper (Piper nigrum L.), cumin (Cuminum cyminum L.), coriander (Coriandrum sativum L.) and cardamom (Elettaria cardamomum) were analyzed for their oil composition by GC-MS. Thirty compounds were identified in the black pepper oil and the main components were β-caryophyllene (20.225 %), sabinene (18.054 %), limonene (16.924 %), α-pinene (9.171 %) and α-phellandrene (5.968 %). Twenty five compounds were identified in the cumin oil – cuminaldehyde (30.834 %), 3-caren-10-al (17.223 %), β-pinene (14.837 %), γ–terpinene (11.928 %), 2-caren-10-al (8.228 %) and pcymene (6.429 %). Twenty nine compounds were identified in the coriander oil – β-linalool (58.141 %), α-pinene (8.731 %), γ-terpinene (6.347 %) and p-cymene (5.227 %). Twenty nine compounds were identified in the cardamom oil – α-terpinyl acetate (39.032 %), eucalyptol (31.534 %), β-linalool (4.829 %), sabinene (4.308 %) and α-terpineol (4.127 %). The antimicrobial activity of essential oils against pathogenic (Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Salmonella sp. (clinical isolate), Staphylococcus aureus ATCC 6538P, Proteus vulgaris G) microorganisms by disc-diffusion method was examined. Gram-positive bacteria were more sensitive to the oils (inhibition zones being between 8 and 12.5 mm) and the minimum inhibitory concentration was more than 600 ppm; Gram-negative bacteria were less sensitive. The obtained essential oils are suitable for use as biopreservative agents.

scholarly journals EFFICACY OF MEDICINAL PLANTS AGAINST HUMAN PATHOGENS ISOLATED FROM WESTERN HIMALAYAS OF HIMACHAL PRADESH

2017 ◽  
Vol 10 (9) ◽  
pp. 353
Author(s):  
Neha Chauhan ◽  
Umar Farooq ◽  
Mohammad Azhar Khan
Keyword(s):  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Juglans Regia ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Phytochemical Analysis ◽  
Chloroform Fraction ◽  
Western Himalayas ◽  
Highly Active ◽  
Antibacterial Assay

  Objective: The present study was aimed to evaluate the antibacterial effect of Himalayan medicinal plants on several human pathogens.Methods: The leaves of Mesua ferrea, Cannabis sativa, and Urtica dioica, bark of Juglans regia, Glycyrrhiza glabra, fruit of Terminalia chebula, and seed of Piper nigrum were dried and powdered before methanolic extraction (ME). Antibacterial assay of MEs was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae using agar well diffusion method. The minimum inhibitory concentrations of these extracts were assessed using microdilution method. The phytochemical analysis of these extracts was carried out to know the bioactive compounds and was fractionated with various solvents such as chloroform, butanol, ethyl acetate, and hexane to isolate the bioactive compound.Results: The ME of C. sativa was found highly active, followed by T. chebula, J. regia, G. glabra, and M. ferrea against these pathogens. While the extracts of U. dioica and P. nigrum found less effective. MIC values of J. regia extract were found much more significant (0.219 g/ml). The antibacterial assay of fractions revealed that the chloroform fraction was highly active against all the pathogens than those of ethyl acetate, butanol, and hexane.Conclusion: It was concluded from the present study that methanol was a good solvent for isolating compounds from medicinal plants and also favors the traditional uses of medicinal plants as antibacterial agents and as a potential source for the new drug discovery. It could also be possible that the compound responsible for antibacterial activity must be present in chloroform fraction of J. regia.

In Vitro Growth of Aragonite Crystal on Nacre Surface

2007 ◽  
Vol 330-332 ◽  
pp. 1335-1338 ◽  
Author(s):  
Cheng Luo ◽  
Lei Xie ◽  
Xiao Xiang Wang
Keyword(s):  
Calcium Carbonate ◽  
Deposition Time ◽  
Early Stage ◽  
Diffusion Method ◽  
In Vitro Growth ◽  
X Ray Diffraction ◽  
X Ray ◽  
Aragonite Crystal ◽  
Diffraction Patterns

In an effort to investigate the growth mechanism of nacre, a conventional vapor diffusion method for CaCO3 crystal precipitation was applied to in vitro growth of calcium carbonate on freshwater H. cumingii Lea shell. In a 10mM/L CaCl2 solution without any additives, aragonite deposit was obtained on the nacre surface, specifically at the edges of nacreous tablets. At the early stage of the deposition (up to 4 hr), the deposit did not take any specific form. After 12hr of deposition, the deposit exhibited faceted morphology characteristic of crystalline. Further increasing the deposition time resulted in the formation of well-faceted crystals of tower-like shape. Both Raman spectra and X-ray diffraction patterns showed that the deposit consists of aragonite polymorph of calcium carbonate.

scholarly journals Sensory acceptability and physical stability evaluation of a prebiotic soy-based dessert developed with passion fruit juice

2012 ◽  
Vol 32 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Daniel Granato ◽  
Maria Lucia Masson ◽  
Jéssica Caroline Bigaski Ribeiro
Keyword(s):  
Soy Protein ◽  
Fruit Juice ◽  
Physical Stability ◽  
Passion Fruit ◽  
Preference Ranking ◽  
Stability Evaluation ◽  
Sensory Acceptability ◽  
Emulsion Process ◽  
Hedonic Scale ◽  
Water Holding

The aim of this study was to use a factorial design approach for developing a palatable and stable soy-based dessert with the addition of Soy Protein (SP), oligofructose, and Passion Fruit Juice (PFJ). Panelists (n = 50) used a seven-point hedonic scale to assess the overall liking, degree of liking of creaminess, taste, and color of the desserts. In addition, the samples were submitted to a preference ranking test in order to evaluate the products' preference. Water Holding Capacity (WHC) and backscattering (BS) measures were also determined to assess the physical stability of the trials. Sample F3 (35% PFJ and 2% SP) was the only one that presented a WHC index of 94.8%; moreover, none of the developed samples had synerisis after 72 hours of storage indicating adequate physical stability of the emulsion process. Samples F2 (25% PFJ, and 3.0% SP), F4 (35% PFJ, and 3.0% SP), and F5 (30% PFJ, and 2.5% SP) presented mean hedonic scores above 'slightly liked' for all sensory attributes. The acceptance index of samples varied from 62.50 to 88% showing the great sensory potential of such products.

Sign in / Sign up

Export Citation Format

Share Document