scholarly journals Antimitotic activity of Piper nigrum on clinical isolates of candida

2019 ◽  
Vol 10 (2) ◽  
pp. 1167-1171
Author(s):  
Gayathri Karthikeyan ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Ethanolic Extract ◽  
Fungal Species ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Disc Diffusion ◽  
Antimitotic Activity ◽  
Diffusion Technique

The objective of this study is to analyse the antimycotic activity of pepper on the clinical isolates of Candida. The extracts were prepared in the following concentrations in sterile water. 5mg/ml and 10mg/ml and 20mg/ml. 100µl of an extract of different concentrations were loaded on sterile filter paper discs measuring 6mm in diameter, so that the concentration of the extract on each disc was 500µg, 1000 µg and 2000µg respectively. The discs were dried and kept aseptically. Screening of antifungal activity [dis diffusion technique] The ethanolic extract of Piper nigrum was screened for antifungal activity by the disc diffusion method. Effect of three different concentrations (2000, 1000, 500,µg /disc) of the ethanolic extract Of Piper nigrum was tested against Candida albicans using disc diffusion technique. All the concentrations of the test solution inhibited the fungal species with varying degree of sensitivity. The inhibitory zone was measured with respect to the different concentration of the extracts which include 2000 µg/disc and 500 µg/disc at both room temperature and cold temperature (4-8°c). The inhibition zones were evaluated at 24 and 48 hrs. The result of this study revealed that the pepper oil has pronounced inhibitory activities against Candida albicans. This result is comparable with studies which have shown that Piper nigrum has a broad antibacterial activity. Since Piper nigrum is easily available and well-tolerated, it can be incorporated into medication for topical antifungal therapy as well.

scholarly journals An evaluation of the antifungal activity of some local medicinal plants against growth of Candida albicans in vitro

2010 ◽  
Vol 9 (2) ◽  
pp. 60
Author(s):  
J.S. Al-Hussaini, and A. M. G. Al-Mohana
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Culture Media ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Elettaria Cardamomum ◽  
Local Plants

The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum, Aloe vera, Thyme Vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in the present study (25, 50, 100, 150, 200, and 400) mg/ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans

scholarly journals Anti-fungal activity of Carica papaya leaf extract against candida albicans and its synergy with fluconazole: an in-vitro study

2020 ◽  
Vol 10 (1) ◽  
pp. 101
Author(s):  
Nilofer Noorie M. ◽  
Chenthamarai G.
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Synergistic Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Diffusion Assay

Background: In this study objectives were to evaluate the antifungal activity in increasing concentrations of ethanolic extract and aqueous extract of Carica papaya against Candida albicans and to assess the synergistic activity of ethanolic extract of Caricia papaya with flucanazole as a potential antifungal.Methods: The aim of the study was to evaluate the antifungal activity in increasing concentrations, 500 µl/ml, 750 µl/ml and 1000 µl/ml of ethanolic extract and aqueous extract of caricia papaya against Candida albicans and the synergistic activity with Fluconazole was assessed by observing the zone of inhibition in agar disc diffusion assay and by observing the turbidity in minimum inhibitory concentration (MIC) assay.Results: It was observed that ethanolic extract of Carica papaya leaf showed significant antifungal activity in higher concentration of 1000µg/ml with zone diameter of 11.97±0.15 mm in disc diffusion assay and MIC of 350 µg/ml. The ethanolic extract of Carica papaya leaf with fluconazole showed synergistic activity with zone diameter of 13.6±0.45 mm in disc diffusion assay and MIC was 125µg/ml, whereas the standard drug Fluconazole’s zone of inhibiton was 12.83±0.9 in disc diffusion and MIC was 500 µg/ml.Conclusions: From this study, we can safely conclude that the Carica papaya leaf extract has a significant antifungal property and exhibit synergistic effect when used with fluconazole.

scholarly journals Antibacterial and Antifungal Activity of Plant Extracts from Spinacia oleraceae L. (Amaranthaceae)

2021 ◽  
pp. 94-100
Author(s):  
Firza Shafique ◽  
Uzma Naureen ◽  
Annam Zikrea ◽  
Sohail Akhter ◽  
Tanzila Rafique ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Bacterial Species ◽  
Ethanolic Extract ◽  
Fungal Species ◽  
Diffusion Method ◽  
Root Extract ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Good Efficiency ◽  
Antibacterial And Antifungal

Spinach (Spinacia oleracea L. SO) leaves represent an important dietary source, have high nutritional value and antimicrobial properties. Spinach leaves have been used in the treatment of human diseases since ancient times. Here, the aim of this study was to evaluate the antimicrobial and antifungal activities of ethanolic extract of Spinach oleracea leaves by determining the minimum inhibitory concentrations (MICs) using well diffusion method against bacterial        species Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus and Klebseilla pneumonia and fungal species Aspergillus fumigatus, Aspergillus niger,        Candida albicans and Fusarium oxysporum. We evaluated from present data that ethanolic extract of root showed zone of inhibition ranges from 6 mm to 21 mm, ethanolic extract of stem showed zone of inhibition ranges from 8 mm to 21 mm and ethanolic extract of leaf showed zone of inhibition ranges from 9 mm to 22 mm from concentration 25 mg/ml to 100mg/ml. Leaf extract has high antibacterial and antifungal activity against bacterial and fungal species while root extract has low antibacterial and antifungal activity against bacterial and fungal species. Activity of plant extract was increased by the increasing concentration of extracts. Very low zone of inhibition was found at concentration 25 mg/1 ml DMSO which ranges from 6 mm to 14 mm while very high zone of inhibition was found at concentration 100 mg/ml which ranges from 6 mm to 22 mm. So, ethanolic extracts of Spinach oleraceae has good efficiency against bacterial and fungal species.

scholarly journals Synthesis and In-vitro Antifungal Evaluation of 5- Pyrazolones

2016 ◽  
Vol 3 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Poonam Gupta ◽  
Jitendra K. Gupta
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Standard Drug ◽  
H Nmr ◽  
Diffusion Technique ◽  
C Nmr

A series of 4-chloro-3-methyl-N-(substituted phenyl)-5-oxo-4,5-dihydro-1H-pyrazole-1- carbothioamide were synthesized using earmark reaction of thiosemicarbazides and ethyl-2-chloroacetoacetate in DMF. All structures of the synthesized compounds were distinguished on the basis of IR,1H-NMR,13C-NMR and also elemental analysis. Synthesized Compounds were examined their potent antifungal activity using disc diffusion technique against three fungal pathogens vizAspergillus niger, Candida albicansandCurvularia. Fluconazole were used as standard drug respectively. All compounds exhibited good to moderate activity.

The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

10.5219/1695 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1112-1119
Author(s):  
Hana Ďúranová ◽  
Veronika Valková ◽  
Lucia Galovičová ◽  
Jana Štefániková ◽  
Miroslava Kačániová
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Vapor Phase ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Wheat Bread ◽  
Citrus Reticulata ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

scholarly journals Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

2014 ◽  
Vol 43 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Cíntia Lima Gouveia ◽  
Isabelle Cristine Melo Freire ◽  
Maria Luísa de Alencar e Silva Leite ◽  
Rebeca Dantas Alves Figueiredo ◽  
Leopoldina de Fátima Dantas de Almeida ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Sodium Bicarbonate ◽  
Fungal Growth ◽  
Antifungal Effect ◽  
Dental Prostheses ◽  
Diffusion Technique ◽  
Zones Of Inhibition ◽  
And Control

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

scholarly journals Evaluation of the anti-mycotic activity of Rosemary Oil Against Candida al-bicans

2019 ◽  
Vol 10 (2) ◽  
pp. 1228-1232
Author(s):  
Shebi S ◽  
Geetha RV ◽  
Lakshmi Thangavelu Lakshmi Thangavelu
Keyword(s):  
Antifungal Activity ◽  
Fungal Growth ◽  
Fungal Species ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Rosemary Oil ◽  
Athlete’S Foot ◽  
Systemic Infections ◽  
Sterile Filter

An antifungal medication, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycoses such as athlete's foot, ringworm, candidiasis, serious systemic infections such as Cryptococcal meningitis, and others. In traditional medicine, extracts and essential oil from flowers and leaves are used in the belief they may be useful to treat a variety of fungal disorders. The aim of this study was to analyse the antimycotic properties of rosemary oil and its principal components. The Rosemary oil was screened for antifungal activity by the disc diffusion method. Activated cultures of Candida albicans in Sabouraud’s broth was adjusted to 0.5 McFarland standards [108cfu/ml]. 100 µl of the inoculum was introduced to molten Sabourauds dextrose agar and poured in the sterile Petri plates and allowed to set. Sterile filter paper discs (6.0 mm diameter) impregnated with 25µl, 50µl and 100µl /disc were placed on fungal seeded plates and incubated at 28oC for 48 hrs. Clear zones within which fungal growth was absent were measured and recorded as the diameter (mm) of complete growth inhibition. All the concentrations of the test solution inhibited the fungal species with varying degree of sensitivity. The extract showed good antifungal activity at different concentrations with a maximum zone of inhibition of 38 mm at concentration 100µl. This study provides a sample large enough to determine the antifungal properties of Rosemary oil and suggests further studies for possible therapeutic use.

scholarly journals GC-MS Analysis and Antibacterial Activity of different Solvent Extracts of Shuteria involucrata

2018 ◽  
Vol 7 (2) ◽  
pp. 116-120
Author(s):  
Senthamizh Selvan N ◽  
◽  
Isaiah S ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Disc Diffusion ◽  
Plant Materials ◽  
Gram Negative ◽  
Good Antibacterial Activity ◽  
Ms Analysis ◽  
Diffusion Technique

The present study was focused to examine the presence of phytoconstituents in the ethanolic extract of Shuteria involucrata plant using GC-MS analysis and Antibacterial activity. The GC-MS analysis of S. involucrata leaf was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. The antimicrobial activity was carried out by disc diffusion technique against the four selected pathogens. Among the four, tested for Antibacterial Activity Staphylococcus aureus, and Pseudomonas aeruginosa and were more susceptible to the extract, whereas the others are less susceptible. Ethanol and methanol extracts of plant materials exhibited good antibacterial activity against gram positive, gram negative bacterias

scholarly journals BIOAKTIVITAS JAMUR Aspergillus flavus YANG BERSIMBION DENGAN ASCIDIAN Eudistoma sp.

2020 ◽  
Vol 8 (2) ◽  
pp. 11
Author(s):  
Alfa Mongi ◽  
Deiske A Sumilat ◽  
Fitje Losung ◽  
Remmy E P Mangindaan ◽  
Rosita A Lintang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Aspergillus Flavus ◽  
Marine Invertebrate ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts

Ascidian is a marine invertebrate that produce bioactive compounds such as antibacterial and antifungal. The purpose of this study was to obtain symbiotic fungi isolates, ethyl acetate extracts from ascidian symbiotic isolates, and to determine the antibacterial and antifungal activities of ethyl acetate extracts of symbiotic isolates using the diffusion method (disc diffusion Kirby and Bauer) against Escherichia coli, Staphylococcus aureus bacteria and against the Candida albicans fungus. The results obtained 1 ascidian species which was cultured to obtain pure isolates, the pure isolates were tested molecularly and the results showed that the isolate was Aspergillus flavus, then the isolates were grown on rice medium and extracted. The results of the fungal extract showed the presence of antibacterial and antifungal activity against Escherichia coli by 10 mm, Staphylococcus aureus by 19 mm and against the Candida albicans by 13.5 mm. Keywords : Ascidian, Eudistoma sp., Aspergillus flavus, Antibacterial, Antifungal, Symbiotic.                                                  AbstrakAscidian merupakan avertebrata laut yang dapat menghasilkan senyawa bioaktif yang seperti antibakteri dan antijamur. Tujuan dari penelitian ini yaitu mendapatkan isolat jamur simbion dari ascidian, mendapatkan ekstrak etil asetat dari isolat jamur simbion ascidian, dan menguji aktivitas antibakteri dan antijamur ekstrak etil asetat isolat jamur simbion dengan metode difusi agar (disc diffusion Kirby and Bauer) terhadap bakteri Escherichia coli, Staphylococcus aureus dan terhadap jamur Candida albicsans. Hasil penelitian didapatkan 1 jenis ascidian yang kemudian dikultur untuk mendapatkan isolat murni, isolat murni diuji secara molekuler dan didapati hasil bahwa isolat tersebut merupakan jamur Aspergillus flavus. Isolat tersebut kemudian ditumbuhkan pada media nasi dan diekstrak. Hasil ekstrak jamur tersebut menunjukkan adanya aktivitas antibakteri dan antijamur terhadap bakteri Escherichia coli sebesar 10 mm, Staphylococcus aureus sebesar 19 mm dan terhadap jamur Candida albicsans 13,5 mm. Kata kunci : Ascidian, Eudistoma sp., Aspergillus flavus, Antibakteri, Antijamur, Simbion.

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