DISTURBANCE OF ANDROSTENEDIOL CONVERTING IN PLACENTA DURING REACTIVATION OF CYTOMEGALOVIRUS INFECTION IN THE FIRST TRIMESTER OF PREGNANCY

2016 ◽  
Vol 1 (61) ◽  
pp. 80-83
Author(s):  
Андриевская ◽  
Irina Andrievskaya ◽  
Шолохов ◽  
Leonid Sholokhov
Keyword(s):  
Cytomegalovirus Infection ◽  
Medical Abortion ◽  
First Trimester ◽  
Igg Antibodies ◽  
Reaction Products ◽  
Avidity Index ◽  
Cytomegalovirus Reactivation ◽  
Hardware Complex ◽  
Enzyme Method ◽  
First Trimester Of Pregnancy

The aim is to analyze androstenediol converting in villous chorions at gestation against cytomegalovirus infection activation during the first trimester of pregnancy. 48 villous chorions samples taken during medical abortion at 8-10th weeks of gestation of women with chronic cytomegalovirus reactivation during pregnancy became the material for study, and 35 villous chorions samples were taken as the control. The results of pregnant women examination were analyzed from the point of cytomegalovirus infection activity with immune-enzyme method by the presence of IgM antibodies or by the value of 4-times and more growth of IgG antibodies titer in paired sera in dynamics in 10 days. IgG avidity index to cytomegalovirus was more than 75% in all women. Androstenediol converting was assessed with histochemical methods. Quantitative evaluation of the reaction products was carried out on sections under the microscope MT (Japan) associated with software-hardware complex «SCION Corporation» (USA). Histochemical reaction product of the androstenediol dehydrogenase was well detected in cyto- and syncytiotrophoblast of chorious villi. Reaction activity in the material from women with reactivation of cytomegalovirus infection during pregnancy was low, which indicated a reduction in the reserve for the synthesis of estrogen. The concentration of estriol and estradiol in villous chorions during reactivation of cytomegalovirus infection decreased till 24.9±2.01 nmol/l and 15765.1±107.77 pmol/l, respectively.

scholarly journals EFFECT OF CYTOMEGALOVIRUS INFECTION ON PROGESTERONE CONVERSION INTO A METABOLITE 5α-PREGNAN-3.20-DIONE IN THE PLACENTA IN EARLY PREGNANCY

2018 ◽  
Vol 1 (67) ◽  
pp. 50-54
Author(s):  
Инна Довжикова ◽  
Inna Dovzhikova ◽  
Ирина Андриевская ◽  
Irina Andrievskaya ◽  
Ксения Петрова ◽  
...  
Keyword(s):  
Nervous System ◽  
Cytomegalovirus Infection ◽  
First Trimester ◽  
Control Group ◽  
Reaction Products ◽  
Latent Stage ◽  
Hardware Complex ◽  
Enzyme Method ◽  
Antibody Titers ◽  
First Trimester Of Pregnancy

The aim was to study the activity of progesterone and 5α-pregnan-3.20-dione conversion in the placenta at the exacerbation of cytomegalovirus infection (CMVI) during the first trimester of pregnancy. The study material included 50 villous chorions taken during medical abortions in the period of 8-10 weeks from women with exacerbation of chronic CMVI during pregnancy. The control group had 35 villous chorions of pregnant women of the same gestation period with chronic CMVI in the latent stage. Diagnosis of CMVI was performed taking into account the activity of CMVI with the immune-enzyme method by the level of antibodies of IgM and antibody titers of IgG in the paired serums in dynamics in 10 days. The progesterone content was determined at the “Stat Fax-2100ˮ spectrophotometer. The activity of 4-pregnene-3.20-dione dehydrogenase and 5α-pregnane-3.20-dione dehydrogenase was assessed by the histochemical method. Quantitative evaluation of the reaction products was carried out on sections under the microscope “Meiji Technoˮ connected to the software-hardware complex “SCION Corporationˮ. It was found out that progesterone content in villous chorions of women with reactivation of CMVI during pregnancy was reduced to 21.5±2.73 nmol/L in comparison with the control group (53.4±3.70 nmol/L, p<0.001). The disturbance of progesterone inversion intensiveness in placenta was proved by the decrease of enzyme activity: of 4-pregnene-3.20-dione dehydrogenase till 15.9±0.47 standard units (in control it was 28.6±0.58 standard units, p<0.001) and of 5α-pregnan-3.20-dione till 16.2±1.11 standard units (in control it was 25.0±1.91 standard units, p<0.001). Reduction in the intensity of progesterone conversion into 5α-dihydroprogesterone (5α-pregnan-3.20-dione) in the placenta with the exacerbation of CMVI can negatively affect the formation and functioning of the fetal nervous system.

scholarly journals DISTURBANCE OF 20α-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN VILLOUS CHORION SYNCIOTHYTHROPHOBLAST AT REACTIVATION OF CYTOMEGALOVIRUS INFECTION DURING PREGNANCY

2017 ◽  
Vol 1 (65) ◽  
pp. 50-53
Author(s):  
Инна Довжикова ◽  
Inna Dovzhikova ◽  
Михаил Луценко ◽  
Mikhail Lutsenko ◽  
Ксения Петрова ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Dehydrogenase Activity ◽  
Cytomegalovirus Infection ◽  
Hydroxysteroid Dehydrogenase ◽  
First Trimester ◽  
Main Group ◽  
Control Group ◽  
Reaction Products ◽  
Igg Antibody ◽  
Antibody Titers

The aim of the work is to assess the effects of cytomegalovirus infection on the 20α-hydroxysteroid dehydrogenase activity in the villous chorion syncytiotrophoblast in the first trimester of pregnancy. The materials for the study included 48 villous chorions samples taken during spontaneous abortions at 8th–10th weeks of gestation from women with reactivation of cytomegalovirus infection (the main group). The control group included 35 villous chorions samples from pregnant women with chronic cytomegalovirus infection in the latent stage taken at medical abortions at the same stage of gestation. The results of women’s examination were studied taken into account the activity of cytomegalovirus infection by immunoassay by detection of IgM antibodies or by 4 times and more increase of IgG antibody titers in the paired serums in dynamics in 10 days. The activity of 20α-hydroxysteroid dehydrogenase was assessed by the histochemical method. Quantitative evaluation of the reaction products was carried out on sections under the microscope MT (Japan) connected to software-hardware complex «SCION Corporation» (USA). At histochemical specimen of villous chorion of pregnant women who had a reactivation of cytomegalovirus infection during pregnancy (main group), there was a decrease of cytophotometric parameter of 20α-hydroxysteroid dehydrogenase activity till 30.1±2.12 pixels/μm2 (p<0.001) compared with the control group. The decrease in the reaction activity in syncytiotrophoblast indicated a decrease in the contents of 20α-dihydroprogesterone in placenta, which contributed, in our opinion, to spontaneous abortion.

scholarly journals CHANGES OF PARAMETRES OF FREE-RADICAL STATUS, OF ANTIOXIDANT PROTECTION AND MORPHOLOGIC CHANGES OF ERYTHROCYTES OF PERIPHERAL BLOOD OF PREGNANT WOMEN OF THE FIRST TRIMESTER WITH CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (68) ◽  
pp. 57-62 ◽  
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Ирина Андриевская ◽  
Irina Andrievskaya
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
Thiobarbituric Acid ◽  
First Trimester ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
First Trimester Of Pregnancy ◽  
Morphologic Changes

The aim is to study the features of changes of free-radical status, antioxidant protection and morphologic changes of erythrocytes of the peripheral blood of pregnant women of the first trimester with exacerbation of cytomegalovirus infection (CMVI). 35 pregnant women with exacerbation of CMVI in the first trimester of gestation (the main group) and 35 pregnant women without this pathology of the same age and term as the patients of the main group were examined. Erythrocytes of the venous blood stabilized by heparine as well as blood plasma became the material of the study. There has been studied the condition of lipid peroxidation (LP) by the contents of diene conjugates and active products reacting with thiobarbituric acid; the state of nonenzymatic antioxidant protection by the contents of α-tocopherol as well as of the enzyme of erythrocytes (superoxide dismutase); morphologic changes of erythrocytes. It was found out that the exacerbation of CMVI in the first trimester of pregnancy is associated with activation of LP processes which include modification of not only primary, reverse stages of this process – the increase of the concentration of diene conjugates (p<0.001), but they lead to an increased formation of secondary active products reacting with thiobarbituric acid (p<0.001), intensifying the destructive action on the membrane of erythrocytes. Primary and secondary products of LP against the decrease of concentration of erythrocyte superoxide dismutase (p<0.001) and α-tocopherol (p<0.001) are accumulated in increasing concentrations and become potential factors of erythrocytes damage causing their functional and metabolic inferiority. Metabolic disturbances and damages of cellular membranes lead to the increase of population of echinocytes, target-like and degenerative forms of erythrocytes, which can be accompanied by a high probability of intravascular hemolysis and contribute to the development of hemic hypoxia complicating the infectious process and the course of pregnancy. Within the present hyperoxidation and suppression of antioxidant protection that lead to the worsening of the rheology of blood, women with exacerbation of CMVI in the first trimester of pregnancy should be prescribed the medicine stabilizing the lipid exchange.

scholarly journals LIPID SYNTHESIS FUNCTION OF LIVER IN PREGNANT WOMEN WITH CYTOMEGALOVIRUS INFECTION

2017 ◽  
Vol 1 (66) ◽  
pp. 54-59
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Наталия Ишутина ◽  
Nataliya Ishutina
Keyword(s):  
Total Cholesterol ◽  
Cytomegalovirus Infection ◽  
Lipid Synthesis ◽  
High Density ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Igg Antibodies ◽  
Avidity Index ◽  
Pregnancy And Delivery ◽  
Antibody Titers

Lipid synthesis function of the liver was studied by the contents of total cholesterol, lipoproteins of high density, lipoproteins of low density and triglycerides in 270 women in the second trimester of pregnancy. The first group (control) included 30 women with a physiological course of pregnancy. The main group consisted of the patients of the second, third, fourth and fifth groups with chronic cytomegalovirus infection (CMVI). Each of them were divided into two subgroups: subgroup A included the women with progressing pregnancy which terminated with the delivery in term; subgroup B - the patients with miscarriage threat whose treatment led to prolongation of the pregnancy and delivery at the 38-40th weeks of gestation. The second group included 60 women with latent chronic CMVI. The third group included patients with reactivation of chronic CMVI (antibody titers of IgM to CMV were 1:200-1:400, antibody titers of IgG to CMV were 1:200-1:400, avidity index of IgG antibodies was more than 65%). The fourth group included 60 patients with acute phase of chronic CMVI (antibody titers of IgM to CMV were 1:200-1:400, antibody titers of IgG to CMV were 1:200-1:800, avidity index of IgG to CMV was more than 65%), the fifth group had 60 patients with reactivation of chronic CMVI (antibody titers of IgM to CMV were 1:200-1:400, antibody titers of IgG to CMV were 1:400-1:1600, avidity index of IgG antibodies was more than 65%). When comparing the parameters of the first group with such-like ones of 2A and 2B subgroups there were not found any changes in the contents of total cholesterol, lipoproteins of high density and lipoproteins of low density and triglycerides; whereas in 3A subgroup there were revealed lower values of lipoproteins of low density (3.55±0.09 mmole/L; p<0.01), and in subgroup 3B there was a decrease of concentration of total cholesterol till 6.33±0.14 mmole/L (in control it was 6.7±0.09 mmole/L; p<0.05) and lipoproteins of low density till 3.43±0.10 mmole/L (in control it was 3.93±0.09 mmole/L; p<0.001). In the patients of subgroup 4B in comparison with the first group there were registered lower values of total cholesterol (6.20±0.15 mmole/L; p<0.01) and there was a growth of lipoproteins of high density till 2.04±0.13 mmole/L (p<0.05). In subgroup 5B there was a drop of concentration of total cholesterol till 5.93±0.14 mmole/L (p<0.001 in comparison with the first group; p<0.05 in comparison with subgroup 2B); there was a decrease of lipoproteins of low density in comparison with the first group till 2.73±0.09 mmole/L (p<0.001), as well as the increase of concentration of lipoproteins of high density till 2.06±0.10 mmole/L (p<0.01) and triglycerides till 1.96±0.05 mmole/L (p<0.01). The changes in the concentration of lipids in the serum of peripheral blood proved a very important role of the acute virus infection with 4-time growth of antibodies of IgG to CMVI in the suppression of lipid synthesis function of the liver and development of miscarriage.

PATHOPHYSIOLOGICAL MECHANISMS OF DAMAGING ACTION OF CYTOMEGALOVIRUS INFECTION IN PREGNANCY

2017 ◽  
Vol 1 (66) ◽  
pp. 98-107 ◽  
Author(s):  
Оксана Кытикова ◽  
Oksana Kytikova ◽  
Татьяна Новгородцева ◽  
Tatyana Novgorodtseva ◽  
Ксения Петрова ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
First Trimester ◽  
Modern Medicine ◽  
Pathophysiological Mechanism ◽  
Negative Consequences ◽  
Childbearing Age ◽  
Pathophysiological Mechanisms ◽  
Long Time ◽  
First Trimester Of Pregnancy ◽  
In Pregnancy

This systematic review was undertaken to define the urgency of studying the problem of cytomegalovirus infection due to the high frequency of detection of specific antibodies among women of childbearing age and the negative consequences of cytomegalovirus for the fetus and newborn. Despite the advances in modern medicine in the study of etiopathogenesis and diagnosis of cytomegalovirus infection during pregnancy, there is currently no trend towards a reduction in the incidence of obstetric pathologies and fetal damage. Physiological features of the functioning of the immune system in pregnancy are discussed; they create the prerequisites for the emergence or activation of long time persistent cytomegalovirus infection and intrauterine fetal damage. Complications in newborns are much more common if the primary cytomegalovirus infection was detected in the first trimester of pregnancy. Cytomegalovirus infection is accompanied by the development of immunodeficiency, but pathophysiological mechanisms of cytomegalovirus infection with the damaging effect in the first trimester of pregnancy need to be detailed. The pathophysiological mechanism of mismatch of immune regulation in pathological pregnancy and in the presence of cytomegalovirus infection is Th-1/Th-2 imbalance which can serve as a target for the development of pathogenetically oriented therapy of pregnant women with cytomegalovirus infection. It is suggested that the specification of these mechanisms will reduce the frequency of obstetric pathologies.

scholarly journals STATE OF LIPID SYNTHESIS FUNCTION OF LIVER AT MODERATE CEREBRAL ISCHEMIA IN NEWBORNS FROM MOTHERS WITH CYTOMEGALOVIRUS INFECTION

2017 ◽  
Vol 1 (65) ◽  
pp. 65-70
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Михаил Луценко ◽  
Mikhail Lutsenko ◽  
Наталия Ишутина ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Total Cholesterol ◽  
Cytomegalovirus Infection ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Igg Antibodies ◽  
Avidity Index ◽  
Igg Antibody ◽  
Igm Antibodies ◽  
Antibody Titers

The contents of total cholesterol, high-density lipoproteins, low-density lipoproteins, and triglycerides in cord blood were studied at moderate cerebral ischemia in 230 mature newborns from mothers with chronic cytomegalovirus infection (CMVI) in the second trimester of gestation (main group). The first group (control group) consisted of 30 newborns from mothers with physiological pregnancy. Depending on the activity of chronic CMVI, 4 groups were distinguished, in each of which there were 2 subgroups. Subgroup A included newborns from mothers with pregnancy and an uncomplicated threat of miscarriage, and in subgroup B there were the patients whose mothers suffered the threat of miscarriage at 16-21st week of gestation. The second group was represented by 50 newborns whose mothers had a latent course of chronic CMVI. The third group included 50 children from mothers with reactivation of chronic CMVI (titers of IgM antibodies to CMV were 1:200-1:400, IgG antibody titers to CMVI were 1:200-1:400, avidity index of IgG antibodies to CMVI was more than 65%). The fourth group included 50 newborns whose mothers suffered an acute phase of chronic CMVI (titers of IgM antibodies to CMV were 1:200-1:400, IgG antibody titers to CMV were 1:200-1: 800, avidity index of IgG antibodies to CMV was more than 65%), and in the fifth group there were 50 patients with antenatal history with reactivation of chronic CMVI (IgM antibody titers to CMV were 1:200-1:400, IgG antibody titers to CMV were 1:400-1:1600, avidity index of IgG antibodies to CMV was more than 65%). When comparing 2A and 2B subgroups with the first group, there were no significant changes in total cholesterol of high and low density lipoproteins and triglycerides. In children in 3A subgroup, the concentration of low density lipoproteins decreased to 0.34±0.02 mmol/L, and in 3B subgroup to 0.32±0.02 mmol/L with simultaneous increase in the triglyceride level to 0.51±0.03 mmol/L (in the first group, it was 0.53±0.03 mmol/L, p<0.001 and 0.43±0.03 mmol/L, p<0.05, respectively). In comparison with the first group in 5B subgroup, the lowest total cholesterol values (1.82±0.05 mmol/L, p<0,05), low-density lipoproteins (0.21±0.05 mmol/L, p<0.001), as well as high triglycerides (0.59±0.03, p<0.001) were registered. These can weaken the compensatory-adaptive reactions of the hormonal system that regulates the immune response, vascular permeability and the blood-brain barrier, as well as disrupt the blood supply to the brain in newborns with moderate cerebral ischemia.

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