scholarly journals Phytochemical Analysis and Antioxidant Potential of Raphia Hookeri Leaf and Epicarp

2018 ◽  
Vol 34 (6) ◽  
pp. 2742-2746
Author(s):  
Oluyori Peter Abimbola ◽  
Dada Adewumi Oluwasogo ◽  
Inyinbor Abosede Adejumoke
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Raphia Hookeri ◽  
Life Threatening ◽  
Phenolic And Flavonoid ◽  
Hydrazyl Radical

The contribution of oxidative stress to the pathogenesis of several health conditions and its association with life-threatening diseases such as malaria has necessitated the search for more phytochemicals with antioxidant properties. Various parts of Raphia hookeri such as the leaves, fruit and sap have been moderately studied and applied in different areas of industry. However, the epicarp is generally discarded. The ethanolic extracts of Raphia hookeri leaf and epicarp were herein investigated for their total phenolic and flavonoid contents using spectrometric methods. 1,1-diphenyl-2-picryl hydrazyl radical (DPPH) and 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) cation assays were used to determine the antioxidant potential of the extracts. The recorded Total Phenolic Content (TPC) of both extracts are comparable while the Total Flavonoid Content of the leaf extract is higher. Consequently, the antioxidant activity of the leaf extract was higher. Although the antioxidant potential of the leaf is higher (351.170±22.950 mg/g RE), the comparable TPC and the antioxidant activity exhibited by Raphia hookeri epicarp extract projects it as a rich phytochemical bio-resourse which may have future relevance in the pharmaceutical industry.

Phytochemical Analysis of Secondary Metabolites and Evaluation of the Antioxidant Activity of Centella asiatica (L.) by UV-Vis Spectroscopy

2020 ◽  
Vol 9 (2) ◽  
pp. 120-126
Author(s):  
Shouvik Chakravarty ◽  
Kaniz W. Sultana ◽  
Sumanta Das ◽  
Indrani Chandra
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Centella Asiatica ◽  
Retention Factor ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Perennial Plant ◽  
Vis Spectroscopy

Background:: Centella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging to the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and saponins. It is used as a traditional medicine for skin infection, cough and asthma. Methods: The present study was intended at the quantitative and qualitative analysis of its secondary metabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according to their Rf (Retention factor) values. Results:: The total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by Folin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract, 44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively). Conclusion:: The present study revealed the antioxidant properties in Centella asiatica.

scholarly journals IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT

2017 ◽  
Vol 10 (6) ◽  
pp. 367
Author(s):  
Samidha M Pawaskar ◽  
Sasangan Kc
Keyword(s):  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Powder ◽  
Phenolic And Flavonoid

Objective: In this study, the leaf powder of Aegle marmelos (L.) Correa. was subjected to preliminary phytochemical and in vitro antioxidant analysis. Methods: The freshly prepared plant leaf extract was subjected to preliminary phytochemical screening, which revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids, and steroids. Reducing power, superoxide (SO) anion radical, nitric oxide (NO) radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the methanolic leaf extract of this plant. The amounts of total phenolic and flavonoid compounds were also determined. Results: This study has revealed that the A. marmelos (L.) Correa. leaf extract showed considerably high amounts of most of the phytochemicals, total antioxidant capacity, total phenolic, and flavonoid content. The study also indicated that the A. marmelos (L.) Correa. showed comparatively good scavenging activity, i.e., inhibition of hydroxyl radical, NO and SO anion scavenging and reducing power activities when compared with the respective standards. Conclusion: The leaf powder of A. marmelos (L.) Correa. can be used as easily accessible source of natural antioxidant and as a possible food supplement or in pharmaceutical industry.

scholarly journals Pharmacognostical, Phytochemical, Antioxidant and Antimicrobial Activity of Costus woodsonii

2019 ◽  
pp. 53-59
Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Concentration Test ◽  
Antioxidant And Antimicrobial Activity

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.

scholarly journals Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Intragastric Administration ◽  
Total Antioxidant ◽  
Liver And Kidney ◽  
Mda Content ◽  
Phenolic And Flavonoid

The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves ofLannea coromandelica(L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various establishedin vitrosystems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activityin vitroand among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC5063.9 ± 0.64 µg/mL), superoxide radical (EC508.2 ± 0.12 mg/mL), and Fe2+chelating activity (EC506.2 ± 0.09 mg/mL). Based on ourin vitroresults, EAF was investigated forin vivoantioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show thatL. coromandelicabared antioxidant activity.

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

scholarly journals Characterisation of the total phenolic, vitamins C and E content and antioxidant properties of the beebread and honey from the same batch

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 158-163
Author(s):  
Tomasz Sawicki ◽  
Natalia Bączek ◽  
Małgorzata Starowicz
Keyword(s):  
Antioxidant Activity ◽  
Vitamin E ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Bioactive Substances ◽  
Reducing Activity ◽  
Phenolic And Flavonoid ◽  
Reducing Potential

The aim of this study was to compare total phenolic and flavonoid content, antioxidant and reducing activity, as well as vitamin C and E content in beebread and honey. The antioxidant activity of samples was determined by photochemiluminescence (PCL) assay, while reducing potential was measured using FRAP and ORAC assays. Vitamin C was analysed by HPLC–MS-TripleTOF method and vitamin E by HPLC-DAD. Beebread was characterised by the higher level of the total phenolic (~90%), flavonoid (~97%), vitamin C (~99%) and E (~98%) as compared to the honey. The data showed that beebread had also higher values of antioxidant activity and reducing potential by ~99% and ~41–98%, respectively, than honey. Our findings indicate that beebread is valuable source of bioactive substances, with the high beneficial properties.

Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

2019 ◽  
Vol 5 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Vinod Kumar Gautam ◽  
Mannu Datta ◽  
Ashish Baldi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Seasonal Variations ◽  
Summer Season ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Spring Season ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

scholarly journals ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC AND FLAVONOID CONTENT VARIATIONS OF LEAF EXTRACTS OF LAURUS NOBILIS L. FROM MOROCCO

2018 ◽  
Vol 11 (12) ◽  
pp. 540 ◽  
Author(s):  
Amal Taroq ◽  
Fatima El Kamari ◽  
Imane Aouam ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laurus Nobilis ◽  
Dry Extract ◽  
Water Extracts ◽  
Phenolic And Flavonoid

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

scholarly journals Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts

2012 ◽  
Vol 66 (4) ◽  
pp. 547-552 ◽  
Author(s):  
Marija Radojkovic ◽  
Zoran Zekovic ◽  
Senka Vidovic ◽  
Drago Kocar ◽  
Pavle Maskovic
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phenolic Components ◽  
Dpph Method ◽  
Morus Spp ◽  
Phenolic And Flavonoid

Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids) and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%). M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g), while highest total flavonoids content (67.37 mg RE/g) was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

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