scholarly journals Short Communication: Sarocladium oryzae associated with sheath rot disease of rice in Indonesia

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Syafiqa Pramunadipta ◽  
Ani Widiastuti ◽  
Arif Wibowo ◽  
Haruhisa Suga ◽  
Achmadi Priyatmojo
Keyword(s):  
Molecular Identification ◽  
Fungal Pathogens ◽  
Short Communication ◽  
Rice Variety ◽  
Leaf Sheath ◽  
Morphological Characterization ◽  
Control Measures ◽  
Rice Grain ◽  
Rot Disease ◽  
Sheath Rot

Abstract. Pramunadipta S, Widiastuti A, Wibowo A, Suga H, Priyatmojo A. 2020. Short Communication: Sarocladium oryzae associated with sheath rot disease of rice in Indonesia. Biodiversitas 21: 1243-1249. One of the obstacles in increasing rice production is the presence of sheath rot pathogen infection, which causes changes in color on the rice sheath to brown or reddish-brown, sometimes does not produce rice grain. The major fungal pathogens that cause sheath rot disease are Sarocladium oryzae and Fusarium spp. The loss of rice yields reaches 85%. The disease found in six provinces, some of which are the largest rice-producing centers in Indonesia. A total of twenty-four Sarocladium sp. were isolated from leaf sheath symptom on potato dextrose agar and water agar medium. Sheath rot pathogen identification based on molecular method was performed using internal transcribed spacer (ITS) rDNA gene sequencing. Necrosis occurs after artificial inoculation in Ciherang rice variety was observed and showed that all isolates were pathogenic. Morphological characterization of the isolates identified them as Sarocladium sp. Molecular identification showed that six representatives isolates belonging to S. oryzae. These findings are important information about the fungal pathogen that causes sheath rot disease in Indonesia, and in studies for formulating control measures of the pathogen in the future to prevent the disease epidemic on rice. This is the first report about the existence of sheath rot disease, morphological characterization and molecular identification of S. oryzae in various rice fields in Indonesia.

scholarly journals Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves

2019 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Endah Yulia ◽  
Silviya Rahma Dhiya Syafira ◽  
Fitri Widiantini ◽  
Wawan Kurniawan
Keyword(s):  
Growth Inhibition ◽  
Leaf Extract ◽  
Plant Protection ◽  
Colony Growth ◽  
Leaf Sheath ◽  
Control Treatment ◽  
Botanical Pesticide ◽  
Plant Pest ◽  
Rot Disease ◽  
Sheath Rot

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot

scholarly journals Metabolomic and Transcriptomic Changes Induced by Potassium Deficiency During Sarocladium oryzae Infection Reveal Insights into Rice Sheath Rot Disease Resistance

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jianglin Zhang ◽  
Zhifeng Lu ◽  
Tao Ren ◽  
Rihuan Cong ◽  
Jianwei Lu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Flag Leaf ◽  
Leaf Sheath ◽  
Lipid Homeostasis ◽  
Antioxidant Systems ◽  
Glutathione S Transferase ◽  
Negative Impacts ◽  
Rot Disease ◽  
The Stability ◽  
Sheath Rot

AbstractRice sheath rot disease caused by Sarocladium oryzae (S. oryzae) infection is an emerging disease, and infection can cause yield losses of 20–85%. Adequate potassium (K) application is a feasible strategy for rice tolerance to S. oryzae infection. However, little is known about the metabolic mechanisms regulated by K that allow rice to cope better with S. oryzae infection. The present study performed a comparative metabolome and transcriptome analysis of rice with different K nutrition statuses before and upon S. oryzae infection. Sarocladium oryzae infection triggered a hydrogen peroxide (H2O2) burst, and K starvation aggravated the accumulation of H2O2 in the flag leaf sheath (FLS), which resulted in lipid peroxidation. Likewise, K deficiency altered the lipid homeostasis of the host plants by hyperaccumulation of 1-alkyl-2-acylglycerophosphoethanolamine. K starvation decreased the content of glycoglycerolipids including monogalactosyldiacyglycerol and digalactosyldoacylglycerol during S. oryzae infection, which destroyed the stability of bilayer membranes. In contrast, sufficient K supply increased antioxidant-related transcript expression (for example, the genes related to glutathione-S-transferase biosynthesis were upregulated), which activated the antioxidant systems. Additionally, upon S. oryzae infection, K starvation amplified the negative impacts of S. oryzae infection on flag leaf photosynthetic potential. These results provide new insight into the role of K in alleviating S. oryzae infection. Adequate K supply decreased the negative impacts of sheath rot disease on rice growth by alleviating lipid peroxidation and maintaining lipid homeostasis.

Control measures of root rot fungal pathogens by producing resistance against twelve wheat cultivars under greenhouse in Nebraska State of USA

2018 ◽  
Vol 51 (2) ◽  
Author(s):  
Tanveer Hussain ◽  
Tony Adesemoye ◽  
Muhammad Ishtiaq ◽  
Mewash Maqbool ◽  
Azhar Azam ◽  
...  
Keyword(s):  
Root Rot ◽  
Fungal Pathogens ◽  
Control Measures ◽  
Wheat Cultivars

Implementing the systems approach in rice pest management : India context

2019 ◽  
Vol 56 (Special Issue) ◽  
pp. 136-142 ◽  
Author(s):  
RK Tanwar ◽  
S Singh ◽  
SP Singh ◽  
Vikas Kumar Kanwar ◽  
Rakesh Kumar ◽  
...  
Keyword(s):  
Systems Approach ◽  
Insect Pests ◽  
Rice Variety ◽  
National Level ◽  
Uttar Pradesh ◽  
Fine Tuning ◽  
Basmati Rice ◽  
Rice Grain ◽  
Integrated Crop Management ◽  
Chemical Pesticides

A basic IPM module for basmati rice as a part of integrated crop management accounting the pest prevalence and the information available from literature has been developed. As the module is location specific and dynamic, therefore needs regular updating and fine tuning depending on the location and pest prevalence. The technology has been successfully validated and implemented during 1998 to 2019 in Pusa Basmati 1, Taraori Basmati, Dehraduni Basmati, Pusa Basmati 1121 in Haryana, Uttar Pradesh and Uttarakhand with little modification and fine tuning. Implementation of the technology resulted in increase in rice grain yield i.e., 21.6% in Pusa Basmati 1 at Shikohpur, in Uttar Pradesh 21.5% in Taraori Basmati at Chhajpur in Haryana, 19.5% in Type 3 in Uttarakhand, 14.5 to 22.7% in Pusa Basmati 1121 in Haryana and 38.2% in Pusa Basmati 1121 in Uttar Pradesh over farmer's practices (FP). In all the basmati rice trials, higher yield as well as Benefit - Cost (B-C) ratio was obtained in IPM as compared to FP. Implementation of IPM led to significant reduction in uses of chemical pesticides. In case of IPM, only 1.46 application of chemical pesticides (103.2 g a.i./ha) were undertaken in Pusa Basmati 1121 against 2.8 application in FP (1214.4 g a.i./ha) at Bambawad, Uttar Pradesh. ICAR-NRRI, Cuttack developed and validated IPM module at Sigmapur (Cuttack, Odisha) non-Basmati rice variety Pooja for four kharif seasons i.e 2010-2013 using standard agronomic practices. The trial resulted in an enhancement of 47.1% yield and 26.47 % B-C ratio over FP. Additional income of Rs. 9857/- per ha was also obtained in IPM over FP. On-Farm trial on validation of IPM module in non-Basmati rice (Pooja variety) under semi deep water conditions conducted in 10 ha during rabi 2017 at Nagapur and Basudeipur villages in Khurda district of Odisha resulted in reduction in the incidence of insect pests and diseases with higher population of natural enemies as compared to FP. Rice yield was recorded 5600 kg ha-1 in IPM as against 4900 kg ha-1 in FP. Recently, ICT based e-pest monitoring and advisory has become an important component for area wise implementation of IPM at state and national level. The programme has been successfully implemented in Odisha during 2010 and 2011 under RKVY. Implementation of the programme had resulted successful management of swarming caterpillar in 13 rice growing districts of Odisha during kharif 2010 and 2011.

scholarly journals Biological control of Phaseolus vulgaris and Pisum sativum root rot disease using Trichoderma species

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Hammad Abdelwanees Ketta ◽  
Omar Abd El-Raouf Hewedy
Keyword(s):  
Phaseolus Vulgaris ◽  
Pisum Sativum ◽  
Common Bean ◽  
Root Rot ◽  
Fungal Pathogens ◽  
Plant Diseases ◽  
Root Rot Disease ◽  
Pea Root ◽  
Rot Disease ◽  
Pea Root Rot

Abstract Background Root rot pathogens reported to cause considerable losses in both the quality and productivity of common bean (Phaseolus vulgaris L.) and pea (Pisum sativum L.). It is an aggressive crop disease with detriment economic influence caused by Fusarium solani and Rhizoctonia solani among other soil-borne fungal pathogens. Destructive plant diseases such as root rot have been managed in the last decades using synthetic pesticides. Main body Seeking of economical and eco-friendly alternatives to combat aggressive soil-borne fungal pathogens that cause significant yield losses is urgently needed. Trichoderma emerged as promising antagonist that inhibits pathogens including those inducing root rot disease. Detailed studies for managing common bean and pea root rot disease using different Trichoderma species (T. harzianum, T. hamatum, T. viride, T. koningii, T. asperellum, T. atroviridae, T. lignorum, T. virens, T. longibrachiatum, T. cerinum, and T. album) were reported both in vitro and in vivo with promotion of plant growth and induction of systemic defense. The wide scale application of selected metabolites produced by Trichoderma spp. to induce host resistance and/or to promote crop yield, may represent a powerful tool for the implementation of integrated pest management strategies. Conclusions Biological management of common bean and pea root rot-inducing pathogens using various species of the Trichoderma fungus might have taken place during the recent years. Trichoderma species and their secondary metabolites are useful in the development of protection against root rot to bestow high-yielding common bean and pea crops.

scholarly journals Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 218
Author(s):  
Chingchai Chaisiri ◽  
Xiang-Yu Liu ◽  
Wei-Xiao Yin ◽  
Chao-Xi Luo ◽  
Yang Lin
Keyword(s):  
Phylogenetic Analyses ◽  
Citrus Fruit ◽  
Epidemiological Studies ◽  
Morphological Characterization ◽  
Jiangxi Province ◽  
Citrus Reticulata ◽  
Fruit Storage ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Morphology Characterization

The Nanfengmiju (Citrus reticulata cv. Nanfengmiju), a high-quality local variety of mandarin, is one of the major fruit crops in Jiangxi Province, China. Citrus melanose and stem-end rot, two common fungal diseases of Nanfengmiju, are both caused by Diaporthe spp. (syn. Phomopsis spp.). Identification of the Diaporthe species is essential for epidemiological studies, quarantine measures, and management of diseases caused by these fungi. Melanose disease was observed on Nanfengmiju fruit in Jiangxi Province of China in 2016. Based on morphological characterization and multi-locus phylogenetic analyses, three out of 39 isolates from diseased samples were identified as D. passifloricola. Since these three isolates did not cause melanose on citrus fruit in the pathogenicity tests, they were presumed to be endophytic fungi present in the diseased tissues. However, our results indicate that D. passifloricola may persist as a symptom-less endophyte in the peel of citrus fruit, yet it may cause stem-end if it invades the stem end during fruit storage. To the best of our knowledge, this is the first report of D. passifloricola as the causal agent of the stem-end rot disease in Citrusreticulata cv. Nanfengmiju.

scholarly journals Comparison of Morphological and Physicochemical Properties of a Floury Rice Variety upon Pre-Harvest Sprouting

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 746
Author(s):  
Chae-Min Han ◽  
Jong-Hee Shin ◽  
Jung-Bae Kwon ◽  
Jong-Soo Kim ◽  
Jong-Gun Won ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Rice Variety ◽  
Wheat Variety ◽  
Economic Loss ◽  
Rice Grain ◽  
Starch Granules ◽  
Rice Varieties ◽  
Starch Structure ◽  
Gelatinization Properties

Pre-harvest sprouting (PHS) severely reduces rice grain yield, significantly affects grain quality, and leads to substantial economic loss. In this study, we aimed to characterize the physicochemical properties and processing quality of the Garumi 2 flour rice variety under PHS conditions and compare them with those of the Seolgaeng, Hangaru, Shingil, and Ilpum rice varieties and the Keumkang wheat variety. Analysis of the molecular structure of starch revealed uniform starch granules, increased proportions of short-chain amylopectin in DP 6–12 (51.0–55.3%), and enhanced crystallinity (30.7–35.7%) in rice varieties for flour compared with the Ilpum cooking rice variety. PHS significantly altered the starch structure and gelatinization properties of Garumi 2. It also caused surface pitting and roughness in Garumi 2 starch granules and decreased their crystallinity. Collectively, the findings of this study provide important novel insights into the effects of PHS on the physicochemical properties of Garumi 2 floury rice for flour.

scholarly journals Community structure of fungal pathogens causing spikelet rot disease of naked oat from different ecological regions of China

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Longlong Liu ◽  
Mingchuan Ma ◽  
Zhang Liu ◽  
Lijun Zhang ◽  
Jianping Zhou
Keyword(s):  
Fungal Pathogens ◽  
The Other ◽  
Qinghai Province ◽  
Naked Oat ◽  
Ecological Regions ◽  
Yield And Quality ◽  
Spray Inoculation ◽  
Grain Yield And Quality ◽  
Rot Disease ◽  
Grain Surface

AbstractSpikelet rot disease (SRD) is an emerging disease of the grain surface of naked oat in China that affects both grain yield and quality. The typical symptom is discoloration from the black structures of the causal fungi. Here, we investigated the fungal communities on the grain surfaces of cultivar Bayou 13 grown in ten ecological oat-producing regions of China, to identify the main pathogens of naked oat SRD. Our results showed that the growth of Alternaria spp. and Davidiella spp. exhibited a competitive relationship and was mainly affected by the elevations of all 10 ecological regions. The dominant pathogens were Davidiella spp. in Shannan Prefecture in Tibet and Haidong Prefecture in Qinghai Province and Alternaria spp. in the other eight regions. The ratios of black pathogens of interest to all pathogens in Shannan Prefecture and Haidong Prefecture were significantly lower than those of the other eight regions, thus indicating that SRD mainly occurred in regions below 2000 m (elevation). We isolated black fungal pathogens from grain surfaces and deduced that they were Alternaria spp. by sequence comparison. The blackened appearance of the grain surfaces was more evident under spray inoculation with a spore suspension of Alternaria than under the control in greenhouse experiments. The recovered pathogen was the same as the pathogen used for inoculation. We thus concluded that Alternaria alone causes naked oat SRD and mainly infects naked oat in regions below 2000 m, which provides a basis for the recognition and management of SRD of naked oat.

Efficacy of Different Level of Systemic Fungicides on Management of Rice Blast at Baitadi, Nepal

2020 ◽  
Vol 7 ◽  
pp. 33-42
Author(s):  
Ashok Acharya ◽  
Prabin Ghimire ◽  
Dhurba Raj Joshi ◽  
Kishor Shrestha ◽  
Govinda Sijapati ◽  
...  
Keyword(s):  
Disease Severity ◽  
Rice Blast ◽  
Fungal Pathogens ◽  
Block Design ◽  
Rice Variety ◽  
Disease Suppression ◽  
Systemic Fungicide ◽  
Disease Development ◽  
Systemic Fungicides ◽  
Different Levels

Rice blast (Pyriculariaoryzae Cavara) is one of the most devastating diseases affecting the rice crop in across the world. Systemic fungicides are used for the suppression of blast diseases caused by fungal pathogens. Propiconazole and Carbendazim are commercial chemical control products available in markets for the control of the fungal pathogen. An experiment was conducted to examine the effectiveness of systemic fungicide on suppression of rice blast incidence in farmers' field during wet seasons in 2016. The treatments consisted of the use of different levels of propiconazole and Carbendazim on ‘Rato Basmati’ a landrace rice variety. The experiments were arranged in a randomized complete block design with three replications. The disease was scored according to the standard scale developed by the International Rice Research Institute (IRRI). Disease severity and Area under Disease Progressive curve (AUDPC) was computed based on that scale score. Propiconazole and Carbendazim at different levels reduce disease development than no treatment (control). But its efficacy was not consistent. The magnitude of disease suppression by Propiconazole was high as compared to Carbendazim. The application of propiconazole at the rate of 1.5 ml effectively reduced disease severity and AUDPC at different dates. So propiconazole at the rate of 1.5 ml thrice at weekly intervals is effective to reduce the disease development

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