scholarly journals Tracheospasmolytic and anti-inflammatory activity of indigenous Curcuma species as traditional antiasthmatic medicines

2018 ◽  
Vol 10 (2) ◽  
pp. 105-110
Author(s):  
SWANDARI PARAMITA ◽  
EMIL BACHTIAR MOERAD ◽  
SJARIF ISMAIL ◽  
EVA MARLIANA
Keyword(s):  
Side Effects ◽  
Guinea Pig ◽  
Curcuma Longa ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Indigenous Species ◽  
Membrane Stabilization ◽  
Major Health Problem ◽  
Anti Inflammatory ◽  
Antiasthma Drugs

Paramita S, Moerad EB, Ismail S, Marliana E. 2018. Tracheospasmolytic and anti-inflammatory activity of indigenous Curcuma species as traditional antiasthmatic medicines. Nusantara Bioscience 10: 105-110. Asthma is still a major health problem in the world. Antiasthma drugs raise the problem of side effects and medical expenses. Thus, it is necessary to develop medicinal plants based antiasthma drugs with fewer side effects and more affordable costs. The potential antiasthma medicinal plant came from the genus of Curcuma (Zingiberaceae family). Turmeric (Curcuma longa L.) is the most prominent species in this genus and has been extensively studied for the treatment of asthma. However, there are other Curcuma indigenous species that are also traditionally used for asthma, namely Curcuma aeruginosa Roxb. and Curcuma mangga Val. Zijp. Both indigenous species have not been studied pharmacological activity as an antiasthma. This research was conducted to know the tracheospasmolytic activity of ethanol extract of C. aeruginosa and C. mangga on the separate organ of guinea pig trachea to determine the antiasthma effect. The research was also conducted to know anti-inflammatory activity with membrane stabilization test and inhibition of lipoxygenase. Results showed that C. aeruginosa and C. mangga had lower tracheospasmolytic activity when they were compared with aminophylline as a positive control. However, the EC50 value of C. aeruginosa (0.020 ± 0.004) and C. mangga (0.039 ± 0.005) were not significantly different with aminophylline (0.016 ± 0.005) as positive controls. The result of membrane stabilization test showed that the EC50 value of C. aeruginosa (47.76 ± 1.57) and C. mangga (67.50 ± 0.97) were high when they were compared with indomethacin (26.39 ± 2.91) as the positive control. Results of lipoxygenase inhibition test showed that the EC50 value of C. aeruginosa (-111.11 ± 2.30) and C. mangga (-101.98 ± 1.32) were high than that of indomethacin (-282.84 ± 7.41) as the positive control. These results show the potential use of C. aeruginosa and C. mangga as antiasthma via tracheospasmolytic activity in separate organs of guinea pig. They also had anti-inflammatory effect based on membrane stabilization and inhibition of lipoxygenase test.

scholarly journals Antiasthmatic effect of Curcuma aeruginosa extract on isolated organ of the trachea

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1799
Author(s):  
Swandari Paramita ◽  
Emil Bachtiar Moerad ◽  
Sjarif Ismail ◽  
Eva Marliana
Keyword(s):  
Side Effects ◽  
Medicinal Plants ◽  
Guinea Pig ◽  
Ethanol Extract ◽  
Relaxation Curve ◽  
Positive Control ◽  
Negative Control ◽  
Spasmolytic Activity ◽  
Major Health Problem ◽  
Antiasthma Drugs

Background:Asthma is a major health problem worldwide. Antiasthma drugs have side effects and can be expensive. It is important to develop antiasthma drugs from medicinal plants that have fewer side effects and are cheaper. One of the medicinal plants used for antiasthma treatment comes fromCurcuma aeruginosa(Zingiberaceaefamily). The aim of the research is to examine spasmolytic activity of ethanol extract ofC. aeruginosaon isolated guinea pig tracheas to determine the antiasthma effects.Methods:The spasmolytic activity ofC. aeruginosaextracts was tested in separated organs of guinea pig trachea. Guinea pig was sacrificed and its trachea rings were suspended in L-shaped wire loops in organ baths containing the Krebs solution aerated with carbogen. Isometric contractions of tracheal rings were measured by the transducer coupled to the amplifier. The trachea rings were exposed to DMSO as negative control, aminophylline as positive control andC. aeruginosaextracts. The single concentration-relaxation curve was obtained in every preparation.Results:The result showed that the decrease of the spasmolytic activity in the guinea pig tracheal tone due toC. aeruginosaextract was significantly better (p=0.022) when compared to the negative control. Meanwhile, the EC50value of aminophylline (0.019 ± 0.05) was not significantly different (p=0.454) withC. aeruginosa(0.024 ± 0.05).Conclusion:It could be concluded thatC. aeruginosaextracts have the potency to be further developed as a new natural source of the antiasthma agents.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF BAWANG DAYAK (ELEUTHERINE BULBOSA (MILL. URB.))ETHANOL BULB EXTRACTS

2019 ◽  
Vol 2 (2) ◽  
pp. 51 ◽  
Author(s):  
Swandari Paramita ◽  
Muhammad Khairul Nuryanto
Keyword(s):  
Side Effects ◽  
Traditional Medicine ◽  
Experimental Method ◽  
Research Method ◽  
Positive Control ◽  
Normal Process ◽  
Membrane Stabilization ◽  
Natural Sources ◽  
East Kalimantan ◽  
Anti Inflammatory

Background: Inflammation is a normal process in response to injury, but causes problems for the patient, including the appearance of pain, swelling, or fever. Anti-inflammatory agents generally used for those conditions, have several side effects to patients. Purpose: The objective of this research was to find alternative anti-inflammatory agents, especially from natural sources. Eleutherine bulbosa (Mill.) Urb. knew as “Bawang Dayak” belong to family Iridaceae is one of the natural sources for anti-inflammatory agents. This plant is known as traditional medicine in East Kalimantan and used as material in this research. Method: The experimental method of anti-inflammatory measurement using membrane stabilization activity for E. Bulbosa ethanol bulbs extracts. Result: The results showed that significant differences of EC50(p < 0.05) were achieved between indomethacin (26.39 ± 2.91) as the positive control with E. Bulbosa (52.87 ± 2.29). EC50 of E. bulbosa showed the potential anti-inflammatory activities with similar effectiveness to half indomethacin concentration. Conclusion: It could be concluded that E. bulbosa could be further developed as a new natural source of the anti-inflammatory agents.

scholarly journals Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1718
Author(s):  
Kaimin Lu ◽  
Jing Zhou ◽  
Jie Deng ◽  
Yangjun Li ◽  
Chuanfang Wu ◽  
...  
Keyword(s):  
Side Effects ◽  
Pathogenic Bacteria ◽  
Periplaneta Americana ◽  
Biochemical Characterization ◽  
Antioxidant Activities ◽  
Inflammatory Activity ◽  
Mice Model ◽  
Acute Colitis ◽  
Anti Inflammatory

The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects. Periplaneta americana (P. americana), a traditional Chinese medicine, has been used to treat arthritis, fever, aches, inflammation, and other diseases. This study aimed to evaluate the anti-inflammatory effects of oligosaccharides from P. Americana (OPA) and its possible mechanisms in vivo. OPA were purified and biochemical characterization was analyzed by HPGPC, HPLC, FT-IR, and GC–MS. Acute colitis mice model was established, the acute toxicity and anti-inflammatory activity were tested in vivo. The results showed OPA with molecular mass of 1.0 kDa were composed of 83% glucose, 6% galactose, 11% xylose, and the backbone was (1→4)-Glcp. OPA had potent antioxidant activities in vitro and significantly alleviated the clinical symptoms of colitis, relieved colon damage without toxic side effects in vivo. OPA exhibited anti-inflammatory activity by regulating Th1/Th2, reducing oxidative stress, preserving intestinal barrier integrity, and inhibiting TLR4/MAPK/NF-κB pathway. Moreover, OPA protected gut by increasing microbial diversity and beneficial bacteria, and reducing pathogenic bacteria in feces. OPA might be the candidate of complementary and alternative medicines of IBD with low-cost and high safety.

Oral anti-hyperalgesic and anti-inflammatory activity of NK1 receptor antagonists in models of inflammatory hyperalgesia of the guinea-pig

Pain ◽  
2000 ◽  
Vol 87 (3) ◽  
pp. 253-263 ◽  
Author(s):  
Elizabeth A. Campbell ◽  
Clive Gentry ◽  
Sadhana Patel ◽  
Bruce Kidd ◽  
Simon Cruwys ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inflammatory Activity ◽  
Nk1 Receptor ◽  
Receptor Antagonists ◽  
Inflammatory Hyperalgesia ◽  
Anti Inflammatory ◽  
Nk1 Receptor Antagonists

scholarly journals Design, Synthesis and Anti-inflammatory Activity of Structurally Simple Anthranilic Acid Congeners Devoid of Ulcerogenic Side Effects

2012 ◽  
Vol 60 (10) ◽  
pp. 1290-1300 ◽  
Author(s):  
Amal Abdel Haleem Mohamed Eissa ◽  
Gamal Abd El-Hakeem Soliman ◽  
Moayad Hussein Khataibeh
Keyword(s):  
Side Effects ◽  
Anthranilic Acid ◽  
Inflammatory Activity ◽  
Design Synthesis ◽  
Anti Inflammatory

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

2021 ◽  
Vol 20 ◽  
Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
Membrane Stabilization ◽  
Leaf Extracts ◽  
Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

scholarly journals ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD

2016 ◽  
Vol 9 (5) ◽  
pp. 78 ◽  
Author(s):  
Entris Sutrisno ◽  
Ketut Adnyana I ◽  
Elin Yulinah Sukandar ◽  
Irda Fidrianny ◽  
Widhya Aligita
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Ethyl Alcohol ◽  
Red Blood Cell ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Alcohol Extract ◽  
Red Blood Cell Membrane ◽  
Anti Inflammatory

ABSTRACTObjective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was thehighest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human redblood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin andasiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit thehemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combinationof ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition ofhemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or ascombinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

scholarly journals UJI AKTIVITAS ANTIINFLAMASI INFUSA BUNGA PUKUL EMPAT SECARA IN VITRO

2021 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
Hidayatul Ihsan ◽  
Iman Surya Pratama ◽  
Nisa Isnaeni Hanifa
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Test Group ◽  
Inflammatory Activity ◽  
Test Results ◽  
Membrane Stabilization ◽  
Red Blood Cell Membrane ◽  
Mirabilis Jalapa ◽  
Anti Inflammatory

The stew of Mirabilis jalapa L. flower has been used traditionally as an anti-inflammatory. Phytochemical screening of Mirabilis jalapa L. flower showed flavonoid content which is known to have anti-inflammatory activity. This study aims to determine the anti-inflammatory activity of Mirabilis jalapa L. flower infusion using the red blood cell membrane stabilization method by inducing hypotonicity and heat. The test group consisted of Mirabilis jalapa L. flower infusion with a concentration of 2.5%; 5%; and 10% w / v, positive control (diclofenac sodium 0.01% w / v and aspirin 0.01% w / v), and negative control (distilled water). The anti-inflammatory activity of Mirabilis jalapa L. flower infusion was determined by the percentage of membrane stabilization and inhibition of red blood cell hemolysis. Data from the test results were statistically analyzed both parametric and nonparametric with SPSS. Mirabilis jalapa L. flower infusion contains flavonoids, tannins and terpenoids. The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% ( p≤0.05). From these results, it can be concluded that the Mirabilis jalapa L. flower infusion has potential as an anti-inflammatory by stabilizing the red blood cell membrane.

scholarly journals Anti-inflammatory activity of Punica granatum extract on oral microbes - In vitro

2019 ◽  
Vol 10 (2) ◽  
pp. 1019-1022
Author(s):  
Westeros Dominic Pereira ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Protein Denaturation ◽  
Periodontal Diseases ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Oral Diseases ◽  
Major Health Problem ◽  
Maximum Inhibition ◽  
Anti Inflammatory ◽  
Oral Microbes

To study the anti-inflammatory effect of Punica granatum extract against the oral microbes. Oral diseases continue to be a major health problem worldwide. Dental caries and periodontal diseases are among the most important global oral health problems, although conditions such as oral and pharyngeal cancers and oral tissue lesions are also significant health concerns. Pomegranate extracts have been used for centuries in traditional medicine to confer health benefits in a number of inflammatory diseases, microbial infections and cancer. The anti-inflammatory activity of pomegranate extract was evaluated by protein denaturation assay, and the results were read spectrophotometrically. Denaturation of proteins is a great‐ documented cause of inflammation. As a part of the investigation on the mechanism of the anti-inflammatory activity, the ability to extract to inhibit protein denaturation was studied. It was effective in inhibiting heat induced albumin denaturation at different concentrations as shown in Table 1. Maximum inhibition, 70.12±1.12% was observed at500µg/ml. IC50 value was found to be 105.35±1.99µg/ml. Aspirin, a standard anti-inflammatory drug showed the maximum inhibition, 77.12±1.42% at the concentration of 200µg/ml. Hence it can be concluded that pomegranate extract has anti-inflammatory property and also can be used in products such as toothpaste and mouth wash etc.

scholarly journals Synthesis, Anti-Inflammatory Activity, and In Silico Study of Novel Diclofenac and Isatin Conjugates

2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Musab Mohamed Ibrahim ◽  
Tilal Elsaman ◽  
Mosab Yahya Al-Nour
Keyword(s):  
Virtual Screening ◽  
Condensation Reaction ◽  
Docking Study ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Molecular Docking Study ◽  
Design Synthesis ◽  
Chemical Structures ◽  
Anti Inflammatory

The design, synthesis, and development of novel non-steroidal anti-inflammatory drugs (NSAIDs) with better activity and lower side effects are respectable area of research. Novel Diclofenac Schiff’s bases (M1, M2, M4, M7, and M8) were designed and synthesized, and their respective chemical structures were deduced using various spectral tools (IR, 1H NMR, 13C NMR, and MS). The compounds were synthesized via Schiff’s condensation reaction and their anti-inflammatory activity was investigated applying the Carrageenan-induced paw edema model against Diclofenac as positive control. Percentage inhibition of edema indicated that all compounds were exhibiting a comparable anti-inflammatory activity as Diclofenac. Moreover, the anti-inflammatory activity was supported via virtual screening using molecular docking study. Interestingly compound M2 showed the highest in vivo activity (61.32% inhibition) when compared to standard Diclofenac (51.36% inhibition) as well as the best binding energy score (-10.765) and the virtual screening docking score (-12.142).

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