Food Fraud Monitoring of Raw Materials for Commercial Seafood Products Using DNA Barcode Information

2021 ◽  
Vol 36 (4) ◽  
pp. 331-341
Author(s):  
Eun-Ji Park ◽  
◽  
Ju-Yeong Kang ◽  
Han-Cheol Lee ◽  
Min-Ji Park ◽  
...  
2021 ◽  
Vol 7 (2) ◽  
pp. 276
Author(s):  
Siti Aimi Sarah Zainal Abidin ◽  
Siti Ayu Jalil ◽  
Mohd Nasir Taib

A globalized food trade, extensive production and complex supply chains are contributing toward an increased number of non-Halal substance in food cases. Halal laboratory testing or Halal testing is an approach to identify contaminants and ensure the integrity of raw materials and food products. The adoption of an integrative approach of Halal management system and Halal testing was investigated to identify and discuss several related economic issues in this field.  From an extensive analysis of academic literatures using ‘Halal Food Fraud’, ‘Halal Food Testing’ and ‘Halal Food’ specifically at food manufacturer, the issues involving economic impact of Halal testing was highlighted. Several Halal Testing Points were proposed for the evaluation of Halal ingredients and products, followed by a conceptual framework on the potential economic impact of Halal food testing. The high demand for transparency in the food industry among consumers has therefore may need food manufacturers to consider Halal food testing as part of their production process.


Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2023
Author(s):  
Suwimol Thariwong ◽  
Aekkhaluck Intharuksa ◽  
Panee Sirisa-ard ◽  
Wannaree Charoensup ◽  
Sunee Chansakaow

The Pikad Tri-phol-sa-mut-than (TS) remedy, a Thai traditional medicine, is officially recorded in Tamra Paetsart Sonkrau Chabub Anurak for its capabilities in treating kidney deficiency. TS remedy is composed of three fruit species—Aegle marmelos (L.) Corrêa., Coriandrum sativum L., and Morinda citrifolia L.—in an equal part by weight. The quality of the raw material is one of the essential factors that can affect the effectiveness and safety of treatment by herbal remedy. The pharmacognostic evaluation and DNA barcode of the three fruit species and TS remedy were performed in this study to authenticate them from contamination, and to provide the scientific database for further uses. Macroscopic and microscopic examination, chemical profile by TLC, and DNA barcoding were employed to positively identify the raw materials bought from the herbal market, especially the powder form. Consequently, the outcomes of this investigation can be used to develop an essential and effective tool for the authentication of crude drugs and herbal remedies.


Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2347-2354
Author(s):  
M.H.M. Yusop ◽  
M.F.A. Bakar

Food product authentication is important at every level of the food manufacturing process, starting from raw materials until finished products. Authentication also plays an important role in assuring accurate food labelling, which is required to help consumers select suitable types of food products. Food adulteration is one of the vital issues addressed by halal authentication, especially for food products that contain pig traces or porcine ingredients. Various methods that aim to guarantee the authenticity of foods have been developed over the past years. In this article, a short review of recent food analytical methods related to authenticity studies, with special regard to pork identification, is provided. The focus of this review is DNA-based methods, which have gained the interest of the scientific community. The specificity, sensitivity and fast and high throughput of the methods are highlighted. In the present case, methods that are capable of detecting pork by using DNA barcode, polymerase chain reaction (PCR)-restriction fragment length polymorphism, conventional PCR, real-time PCR and isothermal amplification are discussed. Although PCR is the most popular method, recent studies have shown that isothermal amplification is a potential alternative because it is rapid, simple and does not require the use of any complicated instruments, such as a thermal cycler and sequencer.


2020 ◽  
Vol 35 (6) ◽  
pp. 574-580
Author(s):  
Eun-Ji Park ◽  
◽  
Ah-Hyeon Jo ◽  
Ju-Yeong Kang ◽  
Han-Cheol Lee ◽  
...  

2016 ◽  
Vol 99 (5) ◽  
pp. 1135-1144 ◽  
Author(s):  
Richard H Stadler ◽  
Lien-Anh Tran ◽  
Christophe Cavin ◽  
Pascal Zbinden ◽  
Erik J M Konings

Abstract A brief overview of the main analytical approaches and practices to determine food authenticity is presented, addressing, as well, food supply chain and future requirements to more effectively mitigate food fraud. Food companies are introducing procedures and mechanisms that allow them to identify vulnerabilities in their food supply chain under the umbrella of a food fraud prevention management system. A key step and first line of defense is thorough supply chain mapping and full transparency, assessing the likelihood of fraudsters to penetrate the chain at any point. More vulnerable chains, such as those where ingredients and/or raw materials are purchased through traders or auctions, may require a higher degree of sampling, testing, and surveillance. Access to analytical tools is therefore pivotal, requiring continuous development and possibly sophistication in identifying chemical markers, data acquisition, and modeling. Significant progress in portable technologies is evident already today, for instance, as in the rapid testing now available at the agricultural level. In the near future, consumers may also have the ability to scan products in stores or at home to authenticate labels and food content. For food manufacturers, targeted analytical methods complemented by untargeted approaches are end control measures at the factory gate when the material is delivered. In essence, testing for food adulterants is an integral part of routine QC, ideally tailored to the risks in the individual markets and/or geographies or supply chains. The development of analytical methods is a first step in verifying the compliance and authenticity of food materials. A next, more challenging step is the successful establishment of global consensus reference methods as exemplified by the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals initiative, which can serve as an approach that could also be applied to methods for contaminants and adulterants in food. The food industry has taken these many challenges aboard, working closely with all stakeholders and continuously communicating on progress in a fully transparent manner.


2019 ◽  
Vol 102 (5) ◽  
pp. 1435-1447 ◽  
Author(s):  
Zhengfei Lu ◽  
Christopher M Thompson ◽  
Tiffany Chua ◽  
Silva Babajanian ◽  
Yanjun Zhang ◽  
...  

Abstract Background: The applications of deoxyribonucleic acid (DNA) barcoding methods have been extended from authenticating taxonomic provenance of animal products to identifying botanicals used as herbal medicine and in botanical dietary supplements. DNA barcoding methods for botanical identification must be adequately validated to meet regulatory compliance. Objective: The goal of this study is to provide a validation protocol for a two-tiered DNA barcoding method that aims to identify raw botanicals. Methods: A barcode database was computationally validated to define the barcode combinations that can unambiguously identify botanicals in the database. A maximum variation sampling technique was used to capture a wide range of perspectives relating to DNA barcode-based botanical identification, including plant parts and species distance, for the experimental validation. Twenty-two authenticated botanicals were purposively sampled from different plant parts—covering both closely related and distantly related species—to validate the two-tiered DNA barcoding method. The performance of the method was assessed on accuracy, precision, ruggedness, and uncertainty. Results: High accuracy (100%) and precision (1.0) were obtained from the validation samples. The method was also found to be rugged and have acceptable uncertainty. Conclusions: The method was validated and suitable for DNA-based identification of botanical raw materials listed in the current database. Highlights: This work will provide support guidance for manufacturers and regulatory policy makers to implement equivalent validated and compliant DNA-based testing in quality control processes to improve botanical raw material identification and authentication.


2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Barahima Abbas ◽  
RUDY JOHANIS KABES ◽  
NOUKE LENDA MAWIKERE ◽  
Reymas Marten Reinhard Ruimassa ◽  
RUDY APRIANTO MATURBONG

Abstract. Abbas B, Kabes RJ, Mawikere NL, Ruimassa RMR, Maturbong RA. 2020. DNA barcode of Metroxylon sagu and other palm species using matK gene. Biodiversitas 21: 4047-4057. Palm family encompasses numerous species, and they disperse broadly across tropical and subtropical countries. The palm family is generally used as an ornamental plant, food, oil, and industrial raw materials. Species of palm that accumulate large amount carbohydrate in the trunk is sago palm (Metroxylon sagu Rottb). The objectives of this study were to explore the plastid sequence associated with matK genes in the palm family and to reveal DNA barcode of 16 genera and 28 species of the palm. Plant materials used in the studies were derived from Sago Research Center (SRC), and other palm sequences were retrieved from the GenBank, NCBI accessions. The PCR product was sequenced by the 1st Base Asia, Singapore. Sequences of the matK gene that were observed in the plastid genome of M. sagu were registered into the NCBI GenBank as DNA barcode of M. sagu. Percent query cover of Blast analysis range from 98% to 100%, and identity range from 97.70% to 100%. Plastid nucleotides associated with matK genes in the chloroplast genome of palm family were shown substantial differences in inter-genera and small differences in inter-species. Genetic distance among palm family range from 0 to 0.026 with nucleotide variation observed was of 0.008822 value. The result of molecular phylogenetic analysis showed that the palm family separated into three clades and three sub-clades based on the plastid matK gene. Species of M. sagu and M. warburgii were considered closely related as compared to other palm species. The matK gene barcoding method is one powerful tool for identification inter-genera and inter-species of the palm.


2020 ◽  
Vol 35 (3) ◽  
pp. 234-242
Author(s):  
Yeon-Cheol Yu ◽  
◽  
Yewon Hong ◽  
Jung Ju Kim ◽  
Dong Ho Lee ◽  
...  

2019 ◽  
Vol 22 (3) ◽  
pp. 508-519
Author(s):  
Asadatun Abdullah ◽  
Hana Aulia Sativa ◽  
Tati Nurhayati ◽  
Mala Nurilmala

Surimi-based processed products are prone to mislabeling using raw materials that are not in accordance with food safety requirements. There were cases reporting use of toxic fish tissue in commercial seafood products. This study was aimed to identify and determine the raw materials used in various processed surimi products using cytochrome oxidase I (COI) gene marker. The experiment consisted of DNA isolation, DNA amplification using several target primers namely full-length barcodes, mini-barcodes, as well as specific species primers against poisonous puffer fish Lagocephalus lunaris and genetic analysis. The results of bioinformatics analysis revealed that S1 samples were Coryphaena hippurus or mahi-mahi fish, S2 and S3 samples were Nemipterus bathybius or curated fish and CS samples were Evynnis cardinalis or kuro fish. Detection of samples with species specific primers of puffer fish Lagocephalus lunaris with annealing temperatures of 54°C, 57°C, and 60°C showed no DNA bands in the six samples analyzed.


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