scholarly journals Single-Laboratory Validation of a Two-Tiered DNA Barcoding Method for Raw Botanical Identification

2019 ◽  
Vol 102 (5) ◽  
pp. 1435-1447 ◽  
Author(s):  
Zhengfei Lu ◽  
Christopher M Thompson ◽  
Tiffany Chua ◽  
Silva Babajanian ◽  
Yanjun Zhang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Raw Materials ◽  
Dna Barcode ◽  
Sampling Technique ◽  
Regulatory Compliance ◽  
Raw Material ◽  
Animal Products ◽  
Policy Makers ◽  
Plant Parts ◽  
Wide Range

Abstract Background: The applications of deoxyribonucleic acid (DNA) barcoding methods have been extended from authenticating taxonomic provenance of animal products to identifying botanicals used as herbal medicine and in botanical dietary supplements. DNA barcoding methods for botanical identification must be adequately validated to meet regulatory compliance. Objective: The goal of this study is to provide a validation protocol for a two-tiered DNA barcoding method that aims to identify raw botanicals. Methods: A barcode database was computationally validated to define the barcode combinations that can unambiguously identify botanicals in the database. A maximum variation sampling technique was used to capture a wide range of perspectives relating to DNA barcode-based botanical identification, including plant parts and species distance, for the experimental validation. Twenty-two authenticated botanicals were purposively sampled from different plant parts—covering both closely related and distantly related species—to validate the two-tiered DNA barcoding method. The performance of the method was assessed on accuracy, precision, ruggedness, and uncertainty. Results: High accuracy (100%) and precision (1.0) were obtained from the validation samples. The method was also found to be rugged and have acceptable uncertainty. Conclusions: The method was validated and suitable for DNA-based identification of botanical raw materials listed in the current database. Highlights: This work will provide support guidance for manufacturers and regulatory policy makers to implement equivalent validated and compliant DNA-based testing in quality control processes to improve botanical raw material identification and authentication.

scholarly journals Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2023
Author(s):  
Suwimol Thariwong ◽  
Aekkhaluck Intharuksa ◽  
Panee Sirisa-ard ◽  
Wannaree Charoensup ◽  
Sunee Chansakaow
Keyword(s):  
Dna Barcoding ◽  
Herbal Remedy ◽  
Raw Materials ◽  
Dna Barcode ◽  
Raw Material ◽  
Morinda Citrifolia ◽  
Herbal Remedies ◽  
Scientific Database ◽  
Kidney Deficiency ◽  
Fruit Species

The Pikad Tri-phol-sa-mut-than (TS) remedy, a Thai traditional medicine, is officially recorded in Tamra Paetsart Sonkrau Chabub Anurak for its capabilities in treating kidney deficiency. TS remedy is composed of three fruit species—Aegle marmelos (L.) Corrêa., Coriandrum sativum L., and Morinda citrifolia L.—in an equal part by weight. The quality of the raw material is one of the essential factors that can affect the effectiveness and safety of treatment by herbal remedy. The pharmacognostic evaluation and DNA barcode of the three fruit species and TS remedy were performed in this study to authenticate them from contamination, and to provide the scientific database for further uses. Macroscopic and microscopic examination, chemical profile by TLC, and DNA barcoding were employed to positively identify the raw materials bought from the herbal market, especially the powder form. Consequently, the outcomes of this investigation can be used to develop an essential and effective tool for the authentication of crude drugs and herbal remedies.

scholarly journals Bio-Based Alternatives to Phenol and Formaldehyde for the Production of Resins

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2237 ◽  
Author(s):  
P. R. Sarika ◽  
Paul Nancarrow ◽  
Abdulrahman Khansaheb ◽  
Taleb Ibrahim
Keyword(s):  
Raw Materials ◽  
Phenol Formaldehyde ◽  
Raw Material ◽  
Wood Industry ◽  
Suitable Material ◽  
The Past ◽  
Wide Range ◽  
Recent Developments ◽  
Sustainable Materials ◽  
Materials Used

Phenol–formaldehyde (PF) resin continues to dominate the resin industry more than 100 years after its first synthesis. Its versatile properties such as thermal stability, chemical resistance, fire resistance, and dimensional stability make it a suitable material for a wide range of applications. PF resins have been used in the wood industry as adhesives, in paints and coatings, and in the aerospace, construction, and building industries as composites and foams. Currently, petroleum is the key source of raw materials used in manufacturing PF resin. However, increasing environmental pollution and fossil fuel depletion have driven industries to seek sustainable alternatives to petroleum based raw materials. Over the past decade, researchers have replaced phenol and formaldehyde with sustainable materials such as lignin, tannin, cardanol, hydroxymethylfurfural, and glyoxal to produce bio-based PF resin. Several synthesis modifications are currently under investigation towards improving the properties of bio-based phenolic resin. This review discusses recent developments in the synthesis of PF resins, particularly those created from sustainable raw material substitutes, and modifications applied to the synthetic route in order to improve the mechanical properties.

scholarly journals PLANT-BASED MILK ANALOGUES IN THE NUTRITION OF PEOPLE WITH NUTRITION-DEPENDENT NON-INFECTIOUS DISEASES

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
N. Pritulska ◽  
I. Motuzka ◽  
A. Koshelnyk ◽  
M. Jarossová ◽  
A. Lacková
Keyword(s):  
Infectious Diseases ◽  
Raw Materials ◽  
Food Products ◽  
Domestic Market ◽  
Biologically Active ◽  
Raw Material ◽  
Fenugreek Seeds ◽  
Product Group ◽  
Wide Range ◽  
Treatment Procedures

A recent tendency in Ukraine is the changes in the structure of food consumption, which are caused by the reduced consumption of some product groups and by their decreasing quality. A persisting problem is that affordable and easy-to-consume food products made with due consideration of the needs of patients with certain categories of diseases can hardly be found on the domestic market. The products present on the domestic market are all imported, available in a limited assortment, very expensive, and not customised to suit all the specific needs of human metabolism. Approaches to the nutrition of patients with certain types of nutrition-dependent and non-infectious diseases have been analysed. It has been established that аn effective component in the diet of patients with non-infectious diseases can be plant-based milk analogues. It has been determined how important it is to use domestic raw materials: it will contribute to expanding domestic production, will help the gross domestic product growth, and will reduce the product’s costs, thus reducing the costs of diagnostic and treatment procedures. It has been established that the assortment of plant-based milk analogues is constantly expanding. The vegetable raw material conventionally used to make this product group has been analysed. It has been shown that fenugreek seeds can be most effectively used to make special food products (in particular, plant-based milk analogues) for the nutritional support of patients with non-infectious diseases. This is feasible due to the availability of the raw material, simple cultivating conditions, the chemical composition of the product (e. g. a wide range of biologically active substances), low costs of the product when it is made from domestic raw materials, and the simple production technology. It is expected that manufacture of milk analogues from fenugreek seeds will widen the assortment of this product group, and satisfy the target consumers’ needs of safe and high quality products that offer an alternative to imports.

scholarly journals Создание гибридов сверхсахарной кукурузы с высоким уровнем антиоксидантной активности с использованием тест-системы на основе стабильного радикала DPPH

2016 ◽  
pp. 50-56
Author(s):  
В. В. Поздняков ◽  
Ю. В. Харченко ◽  
Л. Я. Харченко ◽  
О. В. Анцыферова
Keyword(s):  
Antioxidant Activity ◽  
Functional Food ◽  
Raw Materials ◽  
Raw Material ◽  
Maize Starch ◽  
Wide Range ◽  
Total Antioxidant ◽  
Index Value ◽  
Canning Industry

Гибриды сахарной и сверхсахарной кукурузы являются хорошим сырьем для производства функциональных пищевых продуктов, в том числе диетического и детского питания. Важными показателями высокого качества этих продуктов являются повышенное содержание белка и ценного масла, незначительное количество плохо усвояемого кукурузного крахмала и высокое содержание антиоксидантов, а также отменные вкусовые качества. В работе представлены данные по оценке антиоксидантной активности большой группы новых перспективных гибридов сверхсахарной кукурузы среднеспелой группы, созданных с целью получения ценных источников сырья для консервной промышленности. Значения показателя общей антиоксидантной активности варьировали в широком интервале значений (от 31,8 % до 60,4 %, 568,4–1008 мкг/г семян), что указывает на перспективность использования этого важного биохимического параметра в селекции сверхсахарной кукурузы на качество. Hybrids of excess sugar maize are good raw material for the production of functional food products, including baby food and diet. Important indicators of high quality of these products are high protein content and a valuable oil, a small amount of poorly digestible maize starch and a high content of antioxidants, and also excellent taste qualities. The paper presents data on the evaluation of the antioxidant activity of a large group of promising new hybrids of excess sugar maize of mid-group created in order to obtain valuable raw materials for the canning industry. Index value of total antioxidant activity varied over a wide range (from 31,8 % to 60,4 %, 568,4–1,008 mg/g of seed), which indicates on promising use of this important parameter in the breeding of biochemical excess sugar maize on quality.

Ceramic Composition Material with Mullite Crystals Obtained from the Clayy Raw Material of Yakutia

2020 ◽  
Vol 992 ◽  
pp. 253-258
Author(s):  
M.P. Lebedev ◽  
V.N. Tagrov ◽  
E.S. Lukin
Keyword(s):  
Building Materials ◽  
Raw Materials ◽  
Practical Interest ◽  
Physical And Mechanical Properties ◽  
Ceramic Materials ◽  
Strength Properties ◽  
Raw Material ◽  
Composition Material ◽  
Material Base ◽  
Wide Range

The article deals with the manufacture of modern structural ceramic materials from clay and loam deposits of the Republic of Sakha (Yakutia). The importance and relevance of the development of the production of building materials from local raw materials is emphasized, since this will certainly affect the effectiveness of the construction complex as a whole. The successful development of the construction complex is capable of not only stimulating growth in all sectors of the economy, but also contributes to solving the most pressing social problems. Today, Yakutia has huge reserves of mineral raw materials for the production of a wide range of building materials and products. Of practical interest are wall materials made from clay soils. Given the features of the region’s raw material base, this work focuses on additional processing of traditional material. Controlling the complex physicochemical and structural-mechanical transformations that occur during heat treatment, a methodology has been developed for creating a composite material that will allow competitive innovative materials with enhanced strength properties to be produced with a reinforcing element with a glassy phase matrix of mullite crystals. The fabricated samples have a wide range of physical and mechanical properties and allow using it as a high-quality structural building ceramics, as well as industrial floor technical tile.

scholarly journals Determinants of regulatory compliance in health and social care services: a systematic review protocol

2021 ◽  
Vol 4 ◽  
pp. 13
Author(s):  
Paul Dunbar ◽  
John P. Browne ◽  
Laura O'Connor
Keyword(s):  
Systematic Review ◽  
Quality Improvement ◽  
Social Care ◽  
Service Providers ◽  
Regulatory Compliance ◽  
Policy Makers ◽  
Care Services ◽  
Health And Social Care ◽  
Wide Range ◽  
Study Designs

Background: The delivery of high quality health and social care services is a fundamental goal for health systems worldwide. Identifying the determinants of quality is a complex task as there are a myriad of variables to choose from. Researchers in this field have assessed a range of organisational and environmental factors (for example: staff composition, facility ownership, facility size) for an association with various quality metrics. Less attention has been paid to the determinants of compliance with quality regulation. Identifying the determinants of compliance has the potential to improve regulatory processes and can inform quality improvement initiatives undertaken by service providers and policy makers. This protocol describes a systematic review which will review literature from a wide range of study designs and sources to develop an overview of the determinants of regulatory compliance in health and social care services. Methods: A wide range of study designs and grey literature will be sought for this review. Searches will be conducted using PubMed, MEDLINE, PsycInfo, SocINDEX and CINAHL databases. The studies included in the review will be subject to quality appraisal with reference to the collection of tools available from the Joanna Briggs Institute. Data extraction will be informed by the Consolidated Framework for Implementation Research (CFIR). A narrative synthesis will be conducted on the barriers, facilitators and factors associated with compliance, with reference to the concepts mapped onto the CFIR. GRADE-CERQual will be used to grade the overall body of evidence. Conclusion: The findings of this review will be useful to regulators to inform regulatory policy and practice. Service providers and policy makers may also use the findings to inform quality improvement initiatives aimed at improving compliance and quality across a range of health and social care services.

scholarly journals DNA barcoding identifies cryptic animal tool materials

2021 ◽  
Vol 118 (29) ◽  
pp. e2020699118
Author(s):  
Matthew P. Steele ◽  
Linda E. Neaves ◽  
Barbara C. Klump ◽  
James J. H. St Clair ◽  
Joana R. S. M. Fernandes ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Raw Materials ◽  
Camera Traps ◽  
Behavioral Experiments ◽  
Plant Materials ◽  
Long Term Study ◽  
Fitness Consequences ◽  
Wide Range ◽  
Significant Research ◽  
New Caledonian Crows

Some animals fashion tools or constructions out of plant materials to aid foraging, reproduction, self-maintenance, or protection. Their choice of raw materials can affect the structure and properties of the resulting artifacts, with considerable fitness consequences. Documenting animals’ material preferences is challenging, however, as manufacture behavior is often difficult to observe directly, and materials may be processed so heavily that they lack identifying features. Here, we use DNA barcoding to identify, from just a few recovered tool specimens, the plant species New Caledonian crows (Corvus moneduloides) use for crafting elaborate hooked stick tools in one of our long-term study populations. The method succeeded where extensive fieldwork using an array of conventional approaches—including targeted observations, camera traps, radio-tracking, bird-mounted video cameras, and behavioral experiments with wild and temporarily captive subjects—had failed. We believe that DNA barcoding will prove useful for investigating many other tool and construction behaviors, helping to unlock significant research potential across a wide range of study systems.

scholarly journals Technology of Natural Biocorrectors from Seafood Processing Waste

2021 ◽  
Vol 320 ◽  
pp. 01008
Author(s):  
V.D. Bogdanov ◽  
A.A. Simdyankin ◽  
A.V. Pankina
Keyword(s):  
Nutritional Value ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Chemical Components ◽  
Animal Products ◽  
Biological Value ◽  
Dry Food ◽  
Composition Structure ◽  
Seafood Processing

Biocorrectors are dry concentrates from natural raw sources with high nutritional value due to the high content of important chemical components of food, including essential, minor, and biologically active nutrients. The raw material for the production of natural biocorrectors can be waste from cutting the fish and animal products. It is promising to use the skin of an octopus, the weight of which during cutting is up to 37% of the weight of raw materials, Pacific herring milt, which is formed during cutting in an amount of up to 12.4% of the weight of raw materials, and bivalve mantle mollusk of Primorsky scallop, accounting for about 10% of its mass. The goal of the research is to obtain dry food concentrates from seafood waste that exhibit functional properties. To preserve and form the specific properties of finished products, namely high biological value, digestibility, organoleptic indicators of concentrates, low-processing temperatures (cryotechnology) were used, which maximally preserved the composition, structure and other natural properties of raw materials. The dry seafood concentrates obtained by cryogenic technology exhibit high functionality and can be used as biocorrectors of food in order to impart high nutritional and biological value to it due to the balance of microand macronutrients.

scholarly journals Aktivitas Antimalaria Daun Gempol (Nauclea orientalis (L.) L) terhadap Plasmodium falciparum

2020 ◽  
Vol 30 (2) ◽  
pp. 135-146
Author(s):  
Mery Budiarti ◽  
Anshary Maruzy ◽  
Nengah Ratri RK ◽  
Endang Brotojoyo
Keyword(s):  
Plasmodium Falciparum ◽  
Antimalarial Activity ◽  
Raw Materials ◽  
Raw Material ◽  
Plant Parts ◽  
Phytochemical Compounds ◽  
Bioactive Potential ◽  
Layer Chromatography ◽  
Selection Of

Abstract The use of gempol (Nauclea orientalis (L.) L) stem as a malaria medicine has been empirically and scientifically proven. This condition encourages exploitation which can lead to scarcity of raw materials. Substitution of plant parts as raw material for medicine is one of the innovations that can be done for the sustainability of the plant species. Leaves are one part of the plant that is often used as a raw material for medicine. The selection of leaves as the main ingredient has many advantages over other parts. Until now, research related to the antimalarial bioactive potential of N. orientalis (L.) L leaves has not been published. Therefore, this study aimed to explore the potential for leaf antimalarial activity as an alternative to substitution of stem parts. The leaf extract of N. orientalis (L.) L was prepared by maceration method with 96% ethanol then fractionation was carried out in stage using hexane, ethyl acetate and methanol as solvents. Antimalarial activity testing was carried out in vitro against Plasmodium falciparum 3D7 and Thin Layer Chromatography (TLC) for screening phytochemical compounds in each sample. The hexane solvent was known to produce the most optimum extract by with a yield of 20%. The antimalarial activity of the hexane (IC 50 1.93 μg/mL) and methanol (IC 3.91 μg/ mL) fractions were classified as ‘very active’ and had a tendency to be able to compete with chloroquine phosphate activity. The potential for antimalarial activity in both samples was influenced by the content of alkaloids, steroids, flavonoids and terpenoids which had been developed as active ingredients for malaria drugs. The results of this study indicate that the leaves of Nauclea orientalis (L.) L have the potential to be developed as an alternative to malaria medicine. Abstrak Pemanfaatan batang gempol (Nauclea orientalis (L.) L) sebagai obat malaria telah terbukti secara empiris dan ilmiah. Kondisi tersebut mendorong terjadinya eksploitasi hingga dapat berujung pada kelangkaan bahan baku. Substitusi bagian tumbuhan sebagai bahan baku obat merupakan salah satu inovasi yang dapat dilakukan untuk keberlanjutan hidup spesies tumbuhan tersebut. Daun merupakan salah satu bagian tumbuhan yang sering digunakan sebagai bahan baku obat. Pemilihan daun sebagai bahan utama memiliki banyak kelebihan dibandingkan bagian lainnya. Penelitian terkait potensi bioaktif antimalaria daun Nauclea orientalis (L.) L hingga saat ini belum dipublikasikan. Oleh karena itu, penelitian ini bertujuan untuk menggali potensi aktivitas antimalaria daun sebagai alternatif subtitusi bagian batang. Ekstrak daun Nauclea orientalis (L.) L disiapkan dengan metode maserasi dengan etanol 96%, kemudian dilakukan fraksinasi cair-cair bertingkat menggunakan pelarut heksana, etil asetat, dan metanol. Pengujian aktivitas antimalaria dilakukan secara in vitro terhadap Plasmodium falciparum 3D7 dan Kromatografi Lapis Tipis (KLT) untuk penapisan senyawa fitokimia pada masingmasing sampel. Pelarut heksana diketahui menghasilkan ekstrak paling optimum dengan rendemen 20%. Aktivitas antimalaria fraksi heksana (IC 50 1,93 µg/mL) dan metanol (IC 3,91 µg/mL) yang tergolong dalam kategori ‘sangat aktif, serta memiliki kecenderungan mampu bersaing dengan aktivitas klorokuin fosfat. Potensi aktivitas antimalaria pada kedua sampel tersebut dipengaruhi oleh kandungan senyawa alkaloid, steroid, flavonoid dan terpenoid yang telah banyak dikembangkan sebagai bahan aktif obat malaria. Hasil penelitian ini menunjukkan bahwa daun Nauclea orientalis (L.) L berpotensi untuk dikembangkan sebagai alternatif obat malaria.

scholarly journals Gap-analysis of DNA barcoding in ERMS reference libraries for ascidians and cnidarians

2020 ◽  
Author(s):  
Guy Paz ◽  
Baruch Rinkevich
Keyword(s):  
Quality Control ◽  
Quality Assurance ◽  
Dna Barcoding ◽  
Regulatory Compliance ◽  
Marine Biodiversity ◽  
Sequence Information ◽  
Reference Library ◽  
Knowledge Gaps ◽  
Ecosystem Monitoring ◽  
Wide Range

Abstract BackgroundAll-inclusive DNA barcoding libraries in the storage and analysis platform of the BOLD (Barcode of Life Data) system are essential for the study of the marine biodiversity and are pertinent for regulatory purposes, including ecosystem monitoring and assessment, such as in the context of the EU Water Framework Directive (WFD) and the Marine Strategy Framework Directive (MSFD). Here we investigate knowledge gaps in the lists of DNA barcoded organisms within two inventories, Cnidaria (Anthozoa and Hydrozoa) and Ascidiacea from the reference libraries of the European Register of Marine Species (ERMS) dataset (402 ascidians and 1200 cnidarian species). ERMS records were checked species by species, against publicly available sequence information and other data stored in BOLD system. As the available COI barcode data adequately covers just a small fraction of the ERMS reference library, it is of importance to employ quality control on existing data, to close the knowledge gaps and purge errors off BOLD.ResultsResults revealed that just 22.9% and 29.2% of the listed ascidians and cnidarians species, respectively, are BOLD barcodes of which, 58.4% and 52.3% of the seemingly barcoded species, respectively, were noted to have complete BOLD pages. Thus, only 11.44% of the tunicate and 17.07% of the cnidarian data in the ERMS lists are of high quality. Deep analyses revealed seven common types of gaps in the list of the barcoded species in addition to a wide range of discrepancies and misidentifications, discordances and errors primarily in the GenBank mined data as with the BINs assignments, among others.ConclusionsGap knowledge in barcoding of important taxonomic marine groups exist and in addition, quality management elements (quality assurance and quality control) were not employed when using the list for national monitoring projects, for regulatory compliance purposes and other purposes. Even though BOLD is the most trustable DNA barcoding reference library, worldwide projects of DNA barcoding are needed to close these gaps of mistakes, verifications, missing data and unreliable sequencing labs. Tight quality control and quality assurance is important to close the knowledge gaps of Barcoding of the European recommended ERMS reference library.

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