scholarly journals Physiological properties of new species of Acidithiobacillus isolated from abandoned Tin mine in Ha Thuong, Thai Nguyen province

2014 ◽  
Vol 6 (3) ◽  
pp. 196-200
Author(s):  
Tuyet Anh Nguyen ◽  
Thi Thuy Tuyen Nguyen ◽  
Thi Thuy Duong ◽  
Thi Phuong Quynh Le ◽  
Cuong Tu Hoang
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Ph Value ◽  
Metal Leaching ◽  
Acidithiobacillus Thiooxidans ◽  
Acidic Environment ◽  
16S Rdna Gene ◽  
Acidophilic Bacteria ◽  
Sampling Campaign ◽  
Physiological Properties ◽  
Potential Applications

Acidophilic bacteria are able to tolerate acidic environment and also contribute to the lowering of environmental pH value, implying potential applications in metal-leaching technology extracting metals from tailings and electronic wastes. In this study, we conducted a sampling campaign in abandoned Tin mine in Ha Thuong, Thai Nguyen province, to isolate acidophilic bacteria and to study physiological characteristics of the isolated bacteria. As a result, two acidophilic bacteria were successfully isolated and identification by 16S rDNA gene sequences showed that the two bacteria are similar to Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans (98% and 94% of similarity, respectively). Both strains are tolerant of pH in the range of 3 and have the ability to grow optimally at temperatures of 30°C. Vi khuẩn ưa axit có ý nghĩa ứng dụng trong công nghệ tách rút kim loại từ quặng đuôi và ngay cả từ rác thải linh kiện điện tử. Trong nghiên cứu này, chúng tôi đã tiến hành thu mẫu ở mỏ thiếc bỏ hoang ở Hà Thượng, Thái Nguyên nhằm phân lập được nhóm vi khuẩn ưa axit và qua đó nghiên cứu một số đặc điểm sinh lý của vi khuẩn này. Kết quả chúng tôi đã phân lập được hai chủng vi khuẩn ưa axit. Định dạng bằng nhận diện trình tự gen 16S rADN cho thấy hai vi khuẩn này có độ tương đồng là 98% với vi khuẩn Acidithiobacillus ferrooxidans và 94% Acidithiobacillus thiooxidans. Cả hai chủng vi khuẩn đều có tính chịu pH trong khoảng 3 và có khả năng sinh trưởng tối ưu ở nhiệt độ 30°C.

scholarly journals A Novel Pseudomonas geniculata AGE Family Epimerase/Isomerase and Its Application in d-Mannose Synthesis

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1809
Author(s):  
Zhanzhi Liu ◽  
Ying Li ◽  
Jing Wu ◽  
Sheng Chen
Keyword(s):  
3D Structure ◽  
Three Dimensional ◽  
Optimal Temperature ◽  
Reaction Conditions ◽  
Enzymatic Production ◽  
Physiological Properties ◽  
Potential Applications ◽  
Kinetics Of ◽  
Optimal Reaction ◽  
Gene Age

d-mannose has exhibited excellent physiological properties in the food, pharmaceutical, and feed industries. Therefore, emerging attention has been applied to enzymatic production of d-mannose due to its advantage over chemical synthesis. The gene age of N-acetyl-d-glucosamine 2-epimerase family epimerase/isomerase (AGEase) derived from Pseudomonas geniculata was amplified, and the recombinant P. geniculata AGEase was characterized. The optimal temperature and pH of P. geniculata AGEase were 60 °C and 7.5, respectively. The Km, kcat, and kcat/Km of P. geniculata AGEase for d-mannose were 49.2 ± 8.5 mM, 476.3 ± 4.0 s−1, and 9.7 ± 0.5 s−1·mM−1, respectively. The recombinant P. geniculata AGEase was classified into the YihS enzyme subfamily in the AGE enzyme family by analyzing its substrate specificity and active center of the three-dimensional (3D) structure. Further studies on the kinetics of different substrates showed that the P. geniculata AGEase belongs to the d-mannose isomerase of the YihS enzyme. The P. geniculata AGEase catalyzed the synthesis of d-mannose with d-fructose as a substrate, and the conversion rate was as high as 39.3% with the d-mannose yield of 78.6 g·L−1 under optimal reaction conditions of 200 g·L−1d-fructose and 2.5 U·mL−1P. geniculata AGEase. This novel P. geniculata AGEase has potential applications in the industrial production of d-mannose.

scholarly journals Hydroxyl, Fe2+, and Acidithiobacillus ferrooxidans Jointly Determined the Crystal Growth and Morphology of Schwertmannite in a Sulfate-Rich Acidic Environment

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 3194-3201
Author(s):  
Kun Feng ◽  
Xiaomeng Wang ◽  
Bo Zhou ◽  
Min Xu ◽  
Jianru Liang ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Acidithiobacillus Ferrooxidans ◽  
Acidic Environment

scholarly journals Efecto de la cisteína en un proceso de biodepiritización de carbones en lecho empacado

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Gerardo Andrés Caicedo Pineda ◽  
Marco Antonio Márquez Godoy
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Acidithiobacillus Thiooxidans

<p>Se llevaron a cabo procesos de biodesulfurización de dos carbones colombianos ricos en azufre (“Mina Vieja” y “Vampiro”), en reactores de lecho empacado a nivel de erlenmeyer, utilizando un consorcio de Acidithiobacillus ferrooxidans (ATCC 23270) y Acidithiobacillus thiooxidans (ATCC 15494), evaluando la adición de cisteína a la solución lixiviante. Los ensayos fueron monitoreados por medidas de hierro en solución, pH y potencial redox. Adicionalmente, se hicieron análisis mineralógicos por difracción de rayos X (DRX) antes y después de los experimentos. Los ensayos sin adición de cisteína alcanzaron una oxidación de pirita de 45.3% y 57.9% para “Mina Vieja” y “Vampiro” respectivamente. Cuando se adicionó cisteína, la oxidación aumentó en 14.9% para “Mina Vieja” y 6.4% para “Vampiro”. Por otra parte, todos los ensayos evidenciaron remoción de caolinita, debido a su interacción con el ácido sulfúrico del medio. Con base en los resultados obtenidos, los componentes del carbón influenciaron tanto crecimiento bacteriano como la eficiencia de la cisteína sobre el grado de pirita oxidada.</p>

Acidophilic Microorganisms from Geothermal Copahue Volcano System. Assessment of Biotechnological Applications

2009 ◽  
Vol 71-73 ◽  
pp. 87-91 ◽  
Author(s):  
P. Chiacchiarini ◽  
L. Lavalle ◽  
Alejandra Giaveno ◽  
Edgardo R. Donati
Keyword(s):  
Hot Springs ◽  
Heterotrophic Bacteria ◽  
Extreme Environment ◽  
Acidithiobacillus Thiooxidans ◽  
Biotechnological Applications ◽  
Acidophilic Microorganisms ◽  
Autotrophic Bacteria ◽  
Physiological Properties ◽  
Biological Studies ◽  
System Assessment

This work presents an overview of the physicochemical and biological studies carried out along Rio Agrio and in different hot springs belonging to the geothermal Copahue volcano system, in Neuquén Argentina. This is an extreme environment characterized by wide ranges of temperature, pH (<1 to 8) and heavy metals concentration. In these extreme conditions chemolitho-autotrophic bacteria, archaea, heterotrophic bacteria, yeasts and filamentous fungi were detected. Members of Leptospirillum ferrooxidans, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidianus spp., among others, were successfully cultivated and physiological properties of different isolates were determined. Additionally, bioleaching and biooxidation of regional ores were carried out using mixed native cultures.

scholarly journals The Physicomechanical Deterioration Characteristics and Mesoscopic Damage Analysis of Sandstone under Acidic Environment

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Runke Huo ◽  
Tian Qiu ◽  
Yanling Liang ◽  
Shuguang Li ◽  
Meiting Qian
Keyword(s):  
Elastic Modulus ◽  
Dissolution Rate ◽  
Ph Value ◽  
Acidic Environment ◽  
Quality Change ◽  
Soaking Time ◽  
Rock Samples ◽  
Peak Point ◽  
Function Relationship ◽  
Deterioration Characteristics

The physicomechanical deterioration characteristics of sandstone subjected to H2SO4, HCl, and H2O solutions of different pH values are studied by the method of long-term accelerated immersion. The quantitative relationships between the damage variables based on CT (computer tomographic identification technology) numbers and the immersion time, the uniaxial compressive strength, the peak point strain, and the elastic modulus of rock samples are analyzed. The test results indicate that the pH value of immersion solutions, the dissolution rate of Ca2+ and Na+, and the quality change of rock samples show visible stage characteristics under acidic environment. With the soaking time extended, the pH value of solutions increases gradually, and the quality change of rock samples decreases gradually. The smaller the pH value of immersion solutions is, the higher the dissolution rate of Ca2+ and Na+ is. However, the cation dissolution rate under a weak acid environment with a high pH value has little difference with that under the distilled water (pH = 7). With the increase of the soaking time and the acidity, the compaction stage of rock samples becomes longer, the elastic stage becomes shorter, the deterioration degree of mechanical parameters becomes more extensive, and the destruction of sandstone samples shows ductility characteristics increasingly. The corrosion degree of corroded sandstone samples is quantitatively represented by microscopic damage variables based on CT numbers. The regression analysis results show that damage variables of acid-corroded sandstone samples have a power function relationship with soaking time and an exponential function relationship with peak strength, peak point strain, and elastic modulus.

scholarly journals Responses of Acidithiobacillus thiooxidans A01 to Individual and Joint Nickel (Ni2+) and Ferric (Fe3+)

Minerals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 82 ◽  
Author(s):  
Aijia Chen ◽  
Xiaodong Hao ◽  
Yunhua Xiao ◽  
Kai Zou ◽  
Hongwei Liu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Ferric Iron ◽  
Ph Value ◽  
Sulfur Metabolism ◽  
Metal Resistance ◽  
Acidithiobacillus Thiooxidans ◽  
Promoting Effect ◽  
Maximum Cell Density ◽  
Nickel Ion ◽  
Whole Process

Acidithiobacillus thiooxidans A01 is widely used in bioleaching processes and commonly thrives in most metal-rich environments. However, interactions between different heavy metals remain obscure. In this study, we elaborated the effect of ferric iron on the growth and gene expression of At. thiooxidans A01 under the stress of nickel. The results showed that 600 mM Ni2+ completely inhibited the growth and sulfur metabolism of At. thiooxidans A01. However, trace amounts of Fe3+ (0.5 mM) facilitated the growth of At. thiooxidans A01 in the presence of 600 mM Ni2+. With the addition of 5 mM Fe3+, the maximum cell density reached 1.84 × 108 cell/mL, and pH value was 0.95. In addition, metal resistance-related and sulfur metabolism genes were significantly up regulated with extra ferric iron. Taking the whole process into account, the promoting effect of Fe3+ addition can be attributed to the following: (1) alleviation of the effects of Ni2+ toxicity and restoring the growth of At. thiooxidans A01, (2) a choice of multiple pathways to export nickel ion and producing precursor of chelators of heavy metals. This can suggest that microorganisms may widely exhibit metabolic activity in iron-rich environments with heavy metals. Our study will facilitate the technique development for the processing of ore bodies with highly challenging ore compositions.

Adsorption of water-soluble organic dyes on ferrofluid-modified sawdust

Holzforschung ◽  
2007 ◽  
Vol 61 (3) ◽  
pp. 247-253 ◽  
Author(s):  
Ivo Safarik ◽  
Petra Lunackova ◽  
Ewa Mosiniewicz-Szablewska ◽  
Frantisek Weyda ◽  
Mirka Safarikova
Keyword(s):  
Magnetic Nanoparticles ◽  
Ph Value ◽  
Organic Dyes ◽  
Nile Blue ◽  
Dye Adsorption ◽  
Water Soluble ◽  
Methyl Green ◽  
Brown Crystal ◽  
Potential Applications ◽  
Safranin O

Abstract A magnetically responsive composite material based on lignocellulose and magnetic nanoparticles has been prepared. Spruce sawdust was magnetically modified by contact with water-based magnetic fluid stabilised with perchloric acid. Electron spin resonance and transmission electron microscopy measurements indicated the presence of both individual magnetic nanoparticles and clusters precipitated on the surface of sawdust particles. The material prepared was used to study the adsorption of selected water-soluble organic dyes. The adsorption isotherms followed Langmuir, generalised Freundlich, generalised Langmuir, and Langmuir-Freundlich adsorption patterns. Maximum adsorption capacities for acridine orange, Bismarck brown, crystal violet, malachite green, methyl green, Nile blue, safranin O and Saturn blue LBRR 200 ranged between 34 and 59 mg g-1 dry adsorbent. A change in pH value can increase the dye adsorption. Ferrofluid-modified sawdust is thus an interesting material for further study and potential applications.

Recovery of Nickel and Zinc Using Biogenerated Sulphuric Acid

2009 ◽  
Vol 71-73 ◽  
pp. 649-652 ◽  
Author(s):  
Nadia Yagnentkovsky ◽  
M. Viera ◽  
Edgardo R. Donati
Keyword(s):  
Sulphuric Acid ◽  
Ph Value ◽  
Aerobic Oxidation ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Sulphuric Acid Solution ◽  
Pulp Density ◽  
Solid Matrix ◽  
Attractive Alternative ◽  
Acidithiobacillus Thiooxidans

Sludge generated in automotive and related industries often contains heavy metals. Bioleaching is an attractive alternative for the treatment of metal containing solids. Bacteria of the genus Acidithiobacillus are the most important microorganisms applied to metal solubilisation. These microorganisms are able to produce sulphuric acid from the aerobic oxidation of elemental sulphur. The biogenerated sulphuric acid can be applied to the solubilisation of metals from a solid matrix. In this paper we present the results of our experiments aimed at the removal of nickel and zinc from sludge generated in the water treatment plant of an automotive industry. Acidithiobacillus thiooxidans cells were immobilised on sulphur pearls in a column reactor. The effects of sulphur pulp density and the dilution rate on the production of sulphuric acid were studied. In a second stage, sulphuric acid was used to solubilise the nickel and zinc from the sludge. The effects of different sludge pulp densities and initial acid pH were studied. High recoveries of zinc and nickel were obtained when the pH value of the sulphuric acid solution was lower than 2.0 for 1 and 2 % of pulp density.

Improved Phosphate Ore Leaching with Physical Mutagenized Acidithiobacillus Ferrooxidans Based on Modified Logistic Model

2012 ◽  
Vol 214 ◽  
pp. 520-524 ◽  
Author(s):  
En Wen Wang ◽  
Shao Min Lei ◽  
Wen Qi Gong
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Treatment Time ◽  
Ph Value ◽  
Hot Spring ◽  
Temperature Treatment ◽  
Low Grade ◽  
Initial Growth ◽  
Illumination Time ◽  
Phosphate Ore ◽  
Low Temperature Treatment

Strains of Acidithiobacillus ferrooxidans (At.f) were separated and purified from water sample collected from a hot spring and bioleaching a low grade phosphate ore. The results indicated that bioleaching phosphorus rate increasing sharply was starting at the ending of pH value decreasing, and ascend velocity had slowed down after linear regression of pH was at its second peak. In the environment of ultraviolet mutagenesis, the initial growth of At.f (r) was decreased by illumination time increased, but the maximum rate of bioleaching phosphate ore (ηm) was increased. In the condition of ultrasonic vibration, r was increased by vibration time added, and ηm was almost increased, except 20 min, owing to the modificatory coefficient was large enough. In circumstances of low temperature treatment, r was decreased by treatment time raised; ηm was more increase than the sample of un-treatment (CK).

Surface hydrophobicity of acidophilic heterotrophic bacterial cells in relation to their adhesion on minerals

1991 ◽  
Vol 37 (9) ◽  
pp. 692-696 ◽  
Author(s):  
B. K. Chakrabarti ◽  
P. C. Banerjee
Keyword(s):  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Manganese Nodule ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Bacterial Cells ◽  
Iron Pyrite ◽  
Acidophilic Bacteria ◽  
Hydrophilic Nature

The cell-surface hydrophobicity of acidophilic heterotrophic bacteria originating from mines varied with the pH of the suspending medium and with the growth temperature. Adhesion of these bacterial cells on mineral particles depended upon the hydrophobic (or hydrophilic) nature of both the cells and the minerals. A strong correlation between these properties was usually observed at different pH values of the suspending medium. At a certain pH value, bacterial attachment depended upon the particle size of the minerals. Key words: hydrophobicity, acidophilic bacteria, Acidiphilium cryptum, Acidiphilium symbioticum, adhesion, manganese nodule, chalcopyrite, iron pyrite.

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