Surface hydrophobicity of acidophilic heterotrophic bacterial cells in relation to their adhesion on minerals

1991 ◽  
Vol 37 (9) ◽  
pp. 692-696 ◽  
Author(s):  
B. K. Chakrabarti ◽  
P. C. Banerjee
Keyword(s):  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Manganese Nodule ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Bacterial Cells ◽  
Iron Pyrite ◽  
Acidophilic Bacteria ◽  
Hydrophilic Nature

The cell-surface hydrophobicity of acidophilic heterotrophic bacteria originating from mines varied with the pH of the suspending medium and with the growth temperature. Adhesion of these bacterial cells on mineral particles depended upon the hydrophobic (or hydrophilic) nature of both the cells and the minerals. A strong correlation between these properties was usually observed at different pH values of the suspending medium. At a certain pH value, bacterial attachment depended upon the particle size of the minerals. Key words: hydrophobicity, acidophilic bacteria, Acidiphilium cryptum, Acidiphilium symbioticum, adhesion, manganese nodule, chalcopyrite, iron pyrite.

Fibronectin enhances Campylobacter fetus interaction with extracellular matrix components and INT 407 cells

2008 ◽  
Vol 54 (1) ◽  
pp. 37-47 ◽  
Author(s):  
L. L. Graham ◽  
T. Friel ◽  
R. L. Woodman
Keyword(s):  
Extracellular Matrix ◽  
Solid Phase ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Binding Assays ◽  
Rgd Sequence ◽  
Campylobacter Fetus ◽  
Extracellular Matrix Components

Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface of human colonic biopsy explants, a location consistent with the presence of the extracellular matrix (ECM). To determine whether the ECM mediated C. fetus adherence, 7 C. fetus strains were assessed in a solid-phase binding assay for their ability to bind to immobilized ECM components. Of the ECM components assayed, adherence to fibronectin was noted for all strains. Attachment to ECM components was neither correlated with S-layer expression nor with cell-surface hydrophobicity. Ligand immunoblots, however, identified the S-layer protein as a major site of fibronectin binding, and modified ECM binding assays revealed that soluble fibronectin significantly enhanced the attachment of S-layer-expressing C. fetus strains to other ECM components. Soluble fibronectin also increased C. fetus adherence to INT 407 cells. This adherence was inhibited when INT 407 cells were incubated with synthetic peptides containing an RGD sequence, indicating that integrin receptors were involved in fibronectin-mediated attachment. Together, this data suggests that C. fetus can bind to immobilized fibronectin and use soluble fibronectin to enhance attachment to other ECM components and intestinal epithelial cells. In vivo, fibronectin would promote bacterial adherence, thereby, contributing to the initial interaction of C. fetus with mucosal and submucosal surfaces.

Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa

2020 ◽  
Vol 2 (1) ◽  
pp. 34-41
Author(s):  
Nataliia HRYNCHUK ◽  
Tetiana BUKHTIAROVA ◽  
Daria DUDIKOVA ◽  
Nina VRYNCHANU ◽  
Vira NEDASHKIVSKA ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface Hydrophobicity ◽  
Surface Characteristics ◽  
Surface Hydrophobicity ◽  
Clinical Strain ◽  
Bacterial Cells ◽  
Adhesion Properties ◽  
Minimal Inhibitory Concentrations ◽  
Pre Treatment ◽  
Antibiofilm Agents

Introduction. The present study assessed Pseudomonas aeruginosa surface characteristics, motility and adhesion properties under the influence of 1-[4-(1,1,3,3-tetra methyl butyl) phenoxy]-3-(N-benzyl hexa methylene iminium)-2-propanol chloride (KVM-194). Material and methods. The clinical strain P. aeruginosa 449 was used in the study. The cell surface hydrophobicity (CSH) was evaluated by adhesion to solvent (MATS test). Swimming, swarming and twitching motility of P. aeruginosa were studied by standard methods in media with different agar contents. Cells ability to adhere to polystyrene was assessed by the Christensen method. The effect of KVM-194, meropenem and ciprofloxacin on hydrophobicity and motility was evaluated both at 0.5 or 2.0 minimal inhibitory concentrations (MIC), while on adhesion abilities – only 0.5×MIC. Results. It was shown that 0.5× MIC KVM-194 reduced CSH of P. aeruginosa (by 16%, p˂0.05), affected swimming motility, and decreased its adhesion to polystyrene. The most pronounced changes in adhesion properties were recorded after 3-5 hours of pre-treatment with this compound. Moreover, it was proven that sub-MICs of meropenem and ciprofloxacin did not alter bacterial cells hydrophobicity and had no significant influence on P. aeruginosa motility and adhesion properties. Conclusions. The present study suggested that KVM-194 affected the initial steps of   P. aeruginosa biofilm formation and thus had tremendous potential for new antibiofilm agents’ development.

scholarly journals Diversity of heterotrophic bacteria isolated from biofilm samples and cell surface hydrophobicity

2009 ◽  
Vol 55 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Katsunori Furuhata ◽  
Yuko Kato ◽  
Keiichi Goto ◽  
Motonobu Hara ◽  
Masafumi Fukuyama
Keyword(s):  
Cell Surface ◽  
Heterotrophic Bacteria ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity

Physicochemical, Mechanical, and Molecular Properties of Nonlysogenic and P22-Lysogenic Salmonella Typhimurium Treated with Citrus Oil

2014 ◽  
Vol 77 (5) ◽  
pp. 758-764 ◽  
Author(s):  
JUHEE AHN ◽  
JOSE ALEJANDRO ALMARIO ◽  
SERAJUS SALAHEEN ◽  
DEBABRATA BISWAS
Keyword(s):  
Salmonella Typhimurium ◽  
Expression Patterns ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Swarming Motility ◽  
Heat Treated ◽  
Noticeable Reduction ◽  
Lysogenic Conversion ◽  
Sublethal Concentrations

The aim of this study was to evaluate the phenotypic and genotypic properties of nonlysogenic Salmonella Typhimurium (STP22−) and lysogenic Salmonella Typhimurium (STP22+) in the presence of sublethal concentrations (SLC2D) of citrus essential oils (CEOs), which were used to evaluate antimicrobial susceptibility, cell surface hydrophobicity, autoaggregation ability, bacterial motility, lysogenic conversion, gene expression patterns, and antibiofilm formation. The SLC2D values of non–heat-treated (N-CEO) and heat-treated (H-CEO) CEO in an autoclave at 121°C for 20 min were 2.0 to 2.1 mg/ml against STP22− and 1.7 to 1.9 mg/ml against STP22+. The rates of injured STP22− and STP22+ cells treated with SLC2D of N-CEO and H-CEO ranged from 67 to 83%. The hydrophobicity and autoaggregation were decreased to 2.5 and 19.5% for STP22− and 4.7 and 21.7% for STP22+, respectively, in the presence of N-CEO. A noticeable reduction in the swarming motility was observed in STP22− with N-CEO (14.5%) and H-CEO (13.3%). The numbers of CEO-induced P22 were 5.40 log PFU/ml for N-CEO and 5.65 log PFU/ml for H-CEO. The relative expression of hilA, hilC, hilD, invA, invC, invE, invF, sirA, and sirB was down-regulated in STP22− and STP22+ with N-CEO and H-CEO. The numbers of adherent STP22− and STP22+ were effectively reduced by more than 1 log in the presence of CEO. These results suggest that CEO has potential to be used to control bacterial attachment, colonization, and invasion.

scholarly journals Heterologous Inducible Expression ofEnterococcus faecalis pCF10 Aggregation Substance Asc10 inLactococcus lactis and Streptococcus gordonii Contributes to Cell Hydrophobicity and Adhesion to Fibrin

2000 ◽  
Vol 182 (8) ◽  
pp. 2299-2306 ◽  
Author(s):  
Helmut Hirt ◽  
Stanley L. Erlandsen ◽  
Gary M. Dunny
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Regulatory System ◽  
Surface Hydrophobicity ◽  
Bacterial Cells ◽  
Streptococcus Gordonii ◽  
Cell Surfaces ◽  
Gene Encoding ◽  
Aggregation Substance

ABSTRACT Aggregation substance proteins encoded by the sex pheromone plasmid family of Enterococcus faecalis have been shown previously to contribute to the formation of a stable mating complex between donor and recipient cells and have been implicated in the virulence of this increasingly important nosocomial pathogen. In an effort to characterize the protein further, prgB, the gene encoding the aggregation substance Asc10 on pCF10, was cloned in a vector containing the nisin-inducible nisA promoter and its two-component regulatory system. Expression of aggregation substance after nisin addition to cultures of E. faecalis and the heterologous bacteria Lactococcus lactis andStreptococcus gordonii was demonstrated. Electron microscopy revealed that Asc10 was presented on the cell surfaces ofE. faecalis and L. lactis but not on that ofS. gordonii. The protein was also found in the cell culture supernatants of all three species. Characterization of Asc10 on the cell surfaces of E. faecalis and L. lactisrevealed a significant increase in cell surface hydrophobicity upon expression of the protein. Heterologous expression of Asc10 on L. lactis also allowed the recognition of its binding ligand (EBS) on the enterococcal cell surface, as indicated by increased transfer of a conjugative transposon. We also found that adhesion of Asc10-expressing bacterial cells to fibrin was elevated, consistent with a role for the protein in the pathogenesis of enterococcal endocarditis. The data demonstrate that Asc10 expressed under the control of the nisA promoter in heterologous species will be an useful tool in the detailed characterization of this important enterococcal conjugation protein and virulence factor.

scholarly journals Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

2019 ◽  
Vol 16 (9) ◽  
pp. 1526 ◽  
Author(s):  
Amanda Pacholak ◽  
Wojciech Smułek ◽  
Agnieszka Zgoła-Grześkowiak ◽  
Ewa Kaczorek
Keyword(s):  
Environmental Impact ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Microbial Cell ◽  
Continuous Exposure ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Nitrofuran Derivatives ◽  
Living Organisms ◽  
The Impact

The continuous exposure of living organisms and microorganisms to antibiotics that have increasingly been found in various environmental compartments may be perilous. One group of antibacterial agents that have an environmental impact that has been very scarcely studied is nitrofuran derivatives. Their representative is nitrofurantoin (NFT)—a synthetic, broad-spectrum antibiotic that is often overdosed. The main aims of the study were to: (a) isolate and characterize new microbial strains that are able to grow in the presence of NFT, (b) investigate the ability of isolates to decompose NFT, and (c) study the impact of NFT on microbial cell properties. As a result, five microbial species were isolated. A 24-h contact of bacteria with NFT provoked modifications in microbial cell properties. The greatest differences were observed in Sphingobacterium thalpophilum P3d, in which a decrease in both total and inner membrane permeability (from 86.7% to 48.3% and from 0.49 to 0.42 µM min−1) as well as an increase in cell surface hydrophobicity (from 28.3% to 39.7%) were observed. Nitrofurantoin removal by selected microbial cultures ranged from 50% to 90% in 28 days, depending on the bacterial strain. Although the isolates were able to decompose the pharmaceutical, its presence significantly affected the bacterial cells. Hence, the environmental impact of NFT should be investigated to a greater extent.

scholarly journals Control of Multidrug-Resistant Pathogenic Staphylococci Associated with Vaginal Infection Using Biosurfactants Derived from Potential Probiotic Bacillus Strain

Fermentation ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 19
Author(s):  
Najla Haddaji ◽  
Karima Ncib ◽  
Wael Bahia ◽  
Mouna Ghorbel ◽  
Nadia Leban ◽  
...  
Keyword(s):  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Multidrug Resistant ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Vaginal Infection ◽  
Adhesive Properties ◽  
Coating Agent ◽  
Bacterial Growth Inhibition

Biosurfactants exhibit antioxidant, antibacterial, antifungal, and antiviral activities. They can be used as therapeutic agents and in the fight against infectious diseases. Moreover, the anti-adhesive properties against several pathogens point to the possibility that they might serve as an anti-adhesive coating agent for medical inserts and prevent nosocomial infections, without using synthetic substances. In this study, the antimicrobial, antibiofilm, cell surface hydrophobicity, and antioxidative activities of biosurfactant extracted from Bacillus sp., against four pathogenic strains of Staphylococcus spp. associated with vaginal infection, were studied. Our results have shown that the tested biosurfactant possesses a promising antioxidant potential, and an antibacterial potency against multidrug clinical isolates of Staphylococcus, with an inhibitory diameter ranging between 27 and 37 mm, and a bacterial growth inhibition at an MIC of 1 mg/ mL, obtained. The BioSa3 was highly effective on the biofilm formation of different tested pathogenic strains. Following their treatment by BioSa3, a significant decrease in bacterial attachment (p < 0.05) was justified by the reduction in the optical (from 0.709 to 0.111) following their treatment by BioSa3. The antibiofilm effect can be attributed to its ability to alter the membrane physiology of the tested pathogens to cause a significant decrease (p < 0.05) of over 50% of the surface hydrophobicity. Based on the obtained result of the bioactivities in the current study, BioSa3 is a good candidate in new therapeutics to better control multidrug-resistant bacteria and overcome bacterial biofilm-associated infections by protecting surfaces from microbial contamination.

Relationship of Cell Surface Charge and Hydrophobicity to Strength of Attachment of Bacteria to Cantaloupe Rind†

2002 ◽  
Vol 65 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
DIKE O. UKUKU ◽  
WILLIAM F. FETT
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Interaction Chromatography ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Wash Water ◽  
Bacterial Surface ◽  
E Coli ◽  
Salmonella Infections

The cantaloupe melon has been associated with outbreaks of Salmonella infections. It is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. The surface charge and hydrophobicity of strains of Salmonella, Escherichia coli (O157:H7 and non-O157:H7), and Listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. Initial bacterial attachment to cantaloupe surfaces and the ability of bacteria to resist removal by washing with water were compared with surface charge and hydrophobicity. Whole cantaloupes were submerged in inocula containing individual strains or in cocktails containing Salmonella, E. coli, and L. monocytogenes, either as a mixture of strains containing all three genera or as a mixture of strains belonging to a single genus, for 10 min. Inoculated cantaloupes were dried for 1 h in a biosafety cabinet and then stored for up to 7 days at 4°C. Inoculated melons were washed with water, and bacteria still attached to the melon surface, as well as those in the wash water, were enumerated. Initial bacterial attachment was highest for individual strains of E. coli and lowest for L. monocytogenes, but Salmonella exhibited the strongest attachment on days 0, 3, and 7. When mixed-genus cocktails were used, the relative degrees of attachment of the three genera ware altered. The attachment of Salmonella strains was the strongest, but the attachment of E. coli was more extensive than that of L. monocytogenes on days 0, 3, and 7. There was a linear correlation between bacterial cell surface hydrophobicity (r2 = 0.767), negative charge (r2 = 0.738), and positive charge (r2 = 0.724) and the strength of bacterial attachment to cantaloupe surfaces.

Modulation of Cell Surface Hydrophobicity and Attachment of Bacteria to Abiotic Surfaces and Shrimp by Malaysian Herb Extracts

2012 ◽  
Vol 75 (8) ◽  
pp. 1507-1511 ◽  
Author(s):  
YEW WOH HUI ◽  
GARY A. DYKES
Keyword(s):  
Stainless Steel ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Water Extracts ◽  
Bacterial Hydrophobicity ◽  
Herb Extracts ◽  
Herb Extract

The use of simple crude water extracts of common herbs to reduce bacterial attachment may be a cost-effective way to control bacterial foodborne pathogens, particularly in developing countries. The ability of water extracts of three common Malaysian herbs (Andrographis paniculata, Eurycoma longifolia, and Garcinia atroviridis) to modulate hydrophobicity and attachment to surfaces of five food-related bacterial strains (Bacillus cereus ATCC 14576, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 10145, Salmonella Enteritidis ATCC 13076, Staphylococcus aureus ATCC 25923) were determined. The bacterial attachment to hydrocarbon assay was used to determine bacterial hydrophobicity. Staining and direct microscopic counts were used to determine attachment of bacteria to glass and stainless steel. Plating on selective media was used to determine attachment of bacteria to shrimp. All extracts were capable of either significantly (P &lt; 0.05) increasing or decreasing bacterial surface hydrophobicity, depending on the herb extract and bacteria combination. Bacterial attachment to all surfaces was either significantly (P &lt; 0.05) increased or decreased, depending on the herb extract and bacteria combination. Overall, hydrophobicity did not show a significant correlation (P &gt; 0.05) to bacterial attachment. For specific combinations of bacteria, surface material, and plant extract, significant correlations (R &gt; 0.80) between hydrophobicity and attachment were observed. The highest of these was observed for S. aureus attachment to stainless steel and glass after treatment with the E. longifolia extract (R = 0.99, P &lt; 0.01). The crude water herb extracts in this study were shown to have the potential to modulate specific bacterial and surface interactions and may, with further work, be useful for the simple and practical control of foodborne pathogens.

The role of an extracellular polysaccharide produced by the marine Pseudomonas sp. S9 in cellular detachment during starvation

1989 ◽  
Vol 35 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Michael Wrangstadh ◽  
Patricia L. Conway ◽  
Staffan Kjelleberg
Keyword(s):  
Cell Surface ◽  
Immunofluorescence Microscopy ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Surface ◽  
Extracellular Polysaccharide ◽  
Surface Hydrophobicity ◽  
Bulk Phase ◽  
Bacterial Cells ◽  
Pseudomonas Sp

An exopolysaccharide polymer is produced by the marine Pseudomonas sp. S9 in response to complete energy and nutrient starvation. The presence of this polysaccharide on the cell surface and its subsequent release have been shown to be associated with both adhesion and detachment of the bacterial cells. Detachment from a hydrophobic surface was correlated to the presence of the exopolysaccharide on detached S9 cells. The exopolysaccharide was detected, using immunofluorescence microscopy, on surface-bound cells after only 15 min of exogenous energy and nutrient deprivation. This technique did not reveal any significant amounts of exopolysaccharide on starving bulk phase cells prior to 3 h of starvation. Cells that detached after 5.5 h of starvation had low cell surface hydrophobicity values and increased amounts of cell-bound exopolysaccharide. In contrast, cells that became detached during the first 5.5 h of starvation showed increasing hydrophobicity values during prolonged bulk phase starvation.

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