scholarly journals Genome-wide identification and expression analysis of late embryogenesis abundant protein-encoding genes in rye (Secale cereale L.)

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249757
Author(s):  
Mengyue Ding ◽  
Lijian Wang ◽  
Weimin Zhan ◽  
Guanghua Sun ◽  
Xiaolin Jia ◽  
...  
Keyword(s):  
Secale Cereale ◽  
Abiotic Stresses ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Pcr Analysis ◽  
Genome Wide ◽  
Late Embryogenesis ◽  
Secale Cereale L ◽  
Peg Treatment ◽  
Spatial Specificity

Late embryogenesis abundant (LEA) proteins are members of a large and highly diverse family that play critical roles in protecting cells from abiotic stresses and maintaining plant growth and development. However, the identification and biological function of genes of Secale cereale LEA (ScLEA) have been rarely reported. In this study, we identified 112 ScLEA genes, which can be divided into eight groups and are evenly distributed on all rye chromosomes. Structure analysis revealed that members of the same group tend to be highly conserved. We identified 12 pairs of tandem duplication genes and 19 pairs of segmental duplication genes, which may be an expansion way of LEA gene family. Expression profiling analysis revealed obvious temporal and spatial specificity of ScLEA gene expression, with the highest expression levels observed in grains. According to the qRT-PCR analysis, selected ScLEA genes were regulated by various abiotic stresses, especially PEG treatment, decreased temperature, and blue light. Taken together, our results provide a reference for further functional analysis and potential utilization of the ScLEA genes in improving stress tolerance of crops.

scholarly journals Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 696
Author(s):  
Datong Liu ◽  
Jing Sun ◽  
Dongmei Zhu ◽  
Guofeng Lyu ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Expression Profiles ◽  
Triticum Aestivum L ◽  
Late Embryogenesis Abundant ◽  
Wheat Breeding ◽  
Pcr Analysis ◽  
Lea Genes ◽  
Cellular Dehydration ◽  
Genome Wide ◽  
Late Embryogenesis

Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we carried out a series of analyses based on the latest genome sequence of the bread wheat Chinese Spring. 121 Triticum aestivum L. LEA (TaLEA) genes, classified as 8 groups, were identified and characterized. TaLEA genes are distributed in all chromosomes, most of them with a low number of introns (≤3). Expression profiles showed that most TaLEA genes expressed specifically in grains. By qRT-PCR analysis, we confirmed that 12 genes among them showed high expression levels during late stage grain maturation in two spring wheat cultivars, Yangmai16 and Yangmai15. For most genes, the peak of expression appeared earlier in Yangmai16. Statistical analysis indicated that expression level of 8 genes in Yangmai 16 were significantly higher than Yangmai 15 at 25 days after anthesis. Taken together, our results provide more knowledge for future functional analysis and potential utilization of TaLEA genes in wheat breeding.

scholarly journals KvLEA, a New Isolated Late Embryogenesis Abundant Protein Gene fromKosteletzkya virginicaResponding to Multiabiotic Stresses

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoli Tang ◽  
Hongyan Wang ◽  
Liye Chu ◽  
Hongbo Shao
Keyword(s):  
Abiotic Stresses ◽  
Stress Proteins ◽  
Bioinformatic Analysis ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Lea Proteins ◽  
Lea Gene ◽  
Late Embryogenesis ◽  
Kosteletzkya Virginica ◽  
Multiple Sequences

The LEA proteins are a kind of hydrophilic proteins, playing main functions in desiccation tolerance. However, their importance as a kind of stress proteins in abiotic stress is being clarified little by little. In this study we isolated, cloned, and identified the firstKvLEAgene inKosteletzkya virginica. Bioinformatic analysis showed that the protein encoded by this gene had common properties of LEA proteins and the multiple sequences alignment and phylogenetic analysis further showed that this protein had high homology with twoArabidopsisLEA proteins. Gene expression analysis revealed that this gene had a higher expression in root and it was induced obviously by salt stress. Moreover, the transcripts ofKvLEAwere also induced by other abiotic stresses including drought, high temperature, chilling, and ABA treatment. Among these abiotic stresses, ABA treatment brought about the biggest changes to this gene. Collectively, our research discovered a novel LEA gene and uncovered its involvement in multiabiotic stresses inK. virginica. This research not only enriched studies on LEA gene in plant but also would accelerate more studies onK. virginicain the future.

scholarly journals Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zihan Cheng ◽  
Xuemei Zhang ◽  
Wenjing Yao ◽  
Kai Zhao ◽  
Lin Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Higher Plants ◽  
Abiotic Stress Tolerance ◽  
Gene Families ◽  
Regulatory Elements ◽  
Late Embryogenesis Abundant ◽  
Genome Wide ◽  
Lea Gene ◽  
Late Embryogenesis ◽  
Genome Wide Search

Abstract Background The Late Embryogenesis-Abundant (LEA) gene families, which play significant roles in regulation of tolerance to abiotic stresses, widely exist in higher plants. Poplar is a tree species that has important ecological and economic values. But systematic studies on the gene family have not been reported yet in poplar. Results On the basis of genome-wide search, we identified 88 LEA genes from Populus trichocarpa and renamed them as PtrLEA. The PtrLEA genes have fewer introns, and their promoters contain more cis-regulatory elements related to abiotic stress tolerance. Our results from comparative genomics indicated that the PtrLEA genes are conserved and homologous to related genes in other species, such as Eucalyptus robusta, Solanum lycopersicum and Arabidopsis. Using RNA-Seq data collected from poplar under two conditions (with and without salt treatment), we detected 24, 22 and 19 differentially expressed genes (DEGs) in roots, stems and leaves, respectively. Then we performed spatiotemporal expression analysis of the four up-regulated DEGs shared by the tissues, constructed gene co-expression-based networks, and investigated gene function annotations. Conclusion Lines of evidence indicated that the PtrLEA genes play significant roles in poplar growth and development, as well as in responses to salt stress.

Levels of MeLEA3, a cDNA Sequence Coding for an Atypical Late Embryogenesis Abundant Protein in Cassava, Increase Under In Vitro Salt Stress Treatment

2011 ◽  
Vol 29 (4) ◽  
pp. 997-1005 ◽  
Author(s):  
Carinne de Nazaré Monteiro Costa ◽  
Ailton Borges Santa Brígida ◽  
Bárbara do Nascimento Borges ◽  
Marco Antônio de Menezes Neto ◽  
Luiz Joaquim Castelo Branco Carvalho ◽  
...  
Keyword(s):  
Salt Stress ◽  
Cdna Sequence ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Stress Treatment ◽  
Abundant Protein ◽  
Late Embryogenesis

Translational enhancement conferred by the 3’ untranslated region of a transcript encoding a group 6 late embryogenesis abundant protein

2021 ◽  
Vol 182 ◽  
pp. 104310
Author(s):  
Marina E. Battaglia ◽  
Ana Valeria Martínez-Silva ◽  
Yadira Olvera-Carrillo ◽  
Tzvetanka D. Dinkova ◽  
Alejandra A. Covarrubias
Keyword(s):  
Untranslated Region ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Abundant Protein ◽  
Late Embryogenesis ◽  
Group 6
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