In vitro trichomonocidal potency of Naja nigricollis and Bitis arietans snake venom

Background and Aim: Trichomonas vaginalis drug's limited efficacy and high toxicity, justify the need to explore other therapeutic agents, including animal toxins. In this study, the Naja nigricollis and Bitis arietans snake venoms were used to assess such trichomonocidal effect. Materials and Methods: The median lethal dose (LD50) value for both snake species was calculated by probit analysis using a statistical package for the sciences version 20.0 with an LD50 of 4.04 μg/mL for the N. nigricollis, and no mortality was observed in the B. arietans envenomed rats. Results: The trichomonocidal potency of the snake venom on T. vaginalis was evident with a growth inhibitory concentration of 89% with a half-maximal inhibitory concentration (IC50) of 0.805 μg/mL in B. arietans while 95% for N. nigricollis at an IC50 of 0.411 μg/mL. Conclusion: The statistical analysis of one-way analysis of variance shows a significant difference (p<0.05) between the venoms and positive control group (p<0.001), and there is no significant difference between each venom and its varying concentration (p>0.05). As the least concentration can be useful, interestingly, there is no significant difference in the efficacy of N. nigricollis and B. arietans to T. vaginalis (p>0.05); as such, either of the venom can be used for the treatment of trichomoniasis.

Download Full-text

Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v3 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

Download Full-text

EFEKTIVITAS EKSTRAK DAUN JERUK PURUT (Citrus hystrix) SEBAGAI INSEKTISIDA ALAMI TERHADAP KECOAK AMERIKA (Periplaneta americana) DENGAN METODE SEMPROT

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v8i1.9420 ◽

2020 ◽

Vol 8 (1) ◽

pp. 8-14

Author(s):

Hanina Hanina ◽

Sarah Madeleyne Baringbing

Keyword(s):

Leaf Extract ◽

Periplaneta Americana ◽

Positive Control ◽

Negative Control ◽

Probit Analysis ◽

Observation Time ◽

American Cockroach ◽

Control Group ◽

Significant Difference ◽

Post Test

ABSTRACT Cockroach control can be done in various ways one of which is spraying insecticide. Natural insecticides are less dangerous than conventional insecticides. This study aimed to determined the effectiveness of kaffir lime leaf extract (Citrus hystrix) as a natural insecticide against cockroach americana (Periplaneta americana) with spray method. This research was an experimental study with a post-test only control group designed design. The sample in this study was 60 cockroaches, the sample was divided into 6 groups: negative control (aquades), positive control (transflutrin 0.06%) and and 4 test groups with concentrations of kaffir lime leaf extract (20%, 40%, 60% , and 80%) each group consisted of 10 cockroaches. Phytochemical tests were carried out to determine the content of secondary metabolites in extracts of kaffir lime leaves. Positive phytochemical test results containing flavonoids, alkaloids and essential oils. Based on probit analysis, it is known that the LC50 value = 58.5%; LC95 = 85.1%. The time of death of LT50 is fastest at a concentration of 60% (3,036 hours) and LT95 is the fastest at a concentration of 80% (5,960 hours). Anova test analysis results showed that the positive control group had no significant difference with kaffir lime leaf extract concentration of 80% Kaffir lime leaf extract has an insecticide effect, the higher the concentration of kaffir lime leaf extract, the more number of dead cockroaches and the longer the observation time, the number of cockroaches death will also increase. Keywords: American cockroach, kaffir lime leaf extract, cockroach death ABSTRAK Pengendalian kecoa dapat dilakukan dengan berbagai cara salah satunya penyemprotan insektisida. Insektisida alami kurang berbahaya dibanding insektisida konvensional. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun jeruk purut (Citrus hystrix) sebagai insektisida alami terhadap kecoak americana (Periplaneta americana) dengan metode semprot. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan post-test only control group designed. Sampel dalam penelitian ini adalah 60 ekor kecoak, sampel dibagi menjadi 6 kelompok yaitu kontrol negatif (aquades), kontrol positif (transflutrin 0,06%) dan dan 4 kelompok uji dengan konsentrasi ekstrak daun jeruk purut (20%, 40%, 60%, dan 80%) masing-masing kelompok terdiri dari 10 ekor kecoak. Uji fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder pada ekstrak daun jeruk purut. Hasil uji fitokimia positif mengandung flavonoid, alkaloid dan minyak atsiri. Berdasarkan analisis probit, diketahui nilai LC50 = 58,5%; LC95 = 85,1%. Waktu kematian LT50 paling cepat pada konsentrasi 60% (3,036 jam) dan LT95 paling cepat pada konsentrasi 80% (5,960 jam). Hasil analisis uji anova menunjukkan bahwa kelompok kontrol positif tidak terdapat perbedaan yang signifikan dengan ekstrak daun jeruk purut konsentrasi 80% Ekstrak daun jeruk purut memiliki efek insektisida, semakin tinggi konsentrasi ekstrak daun jeruk purut maka jumlah kecoak yang mati semakin banyak dan semakin lama waktu pengamatan, maka jumlah kecoak yang mati juga akan semakin banyak. Kata kunci : kecoak amerika, ekstrak daun jeruk purut, kematian kecoak

Download Full-text

Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090201 ◽

2011 ◽

Vol 9 (2) ◽

pp. 33-37

Author(s):

MUHAMMAD ARIF NUR SYAHID ◽

CR. SITI UTARI ◽

SUTARMIADJI DJUMARGA

Keyword(s):

Ascaris Suum ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Mimosa Pudica ◽

Control Group Design ◽

Significance Level ◽

Death Time ◽

Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

Download Full-text

Evaluation of the Apical Sealing Ability of Resin and Silicon Based Sealers by Dye Extraction Method: An In Vitro Study

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/46152.14428 ◽

2021 ◽

Author(s):

Abbas Saifuddin Unwala ◽

Vanitha Umesh Shenoy ◽

Sumanthini V Margasahayam ◽

Shouvik S Mandal

Keyword(s):

In Vitro Study ◽

Positive Control ◽

Control Group ◽

P Value ◽

Dye Penetration ◽

Significant Difference ◽

Ah Plus ◽

The Mean ◽

Dye Extraction

Introduction: Thorough, chemo-mechanical preparation of the root canal is required to completely eliminate microorganisms from the dentinal tubules. Faulty obturation in the apical area is the pathway from which bacteria and their toxins pass on to the periapical area and this leakage can be studied by sealability studies. Aim: To evaluate the apical sealing ability of three sealers i.e., AH Plus, Roekoseal and Adseal using passive dye penetration test and a dye extraction test with Methylene blue dye to evaluate the apical leakage. Materials and Methods: The present study was an experimental in-vitro study, carried out at MGM Dental College and Hospital, Navi Mumbai, Maharashtra, India over a period of 10 months (from August 2013 to May 2014). Eighty freshly extracted human permanent maxillary central incisor teeth were obtained. The crowns of the teeth were sectioned at/below cemento-enamel junction and divided into five groups: Groups I, II and III were obturated with gutta-percha and three different sealers, and groups IV and V were the two control groups. Passive dye penetration test was performed to evaluate penetration of the dye by means of capillarity followed by dye extraction test. At the end of dye extraction procedure, the supernatant solution obtained was analysed using an Ultraviolet spectrophotometer. Significance was set at 5%. Statistical analysis was performed by using IBM Statistical Package for the Social Sciences (SPSS) statistical software version 20 (Armonk, New York, United States). The normality of data in each group was tested using Shapiro-Wilk test. The mean absorbance values of the five groups were compared using one-way ANOVA. Further to compare the level of absorbance of dye among various groups, Tukey’s post-hoc test was applied. Results: The mean values of dye absorbance of all groups were as follows, AH Plus (0.02638), Roekoseal (0.06713), Adseal (0.03575), positive control group (1.36250) and negative control group (0.01894). One-way ANOVA showed p<0.001, thus indicating there was a significant difference between the dye absorbance values of all the groups. Tukey’s post-hoc test showed statistically significant difference in dye absorbance of the experimental groups when compared to positive control group. There was no significant difference in dye absorbance when experimental groups were compared to each other. When AH Plus was compared with Roekoseal, the p-value was 0.568 and 0.997 with Adseal whereas when Roekoseal was compared with Adseal the p-value was 0.778. Conclusion: The mean dye absorbance value of AH Plus was found to be least and that of Roekoseal was highest in the experimental groups. AH Plus gave the best results, as least dye was extracted from this group, indicating least dye penetration and minimum microleakage followed by Adseal and Roekoseal.

Download Full-text

Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v2 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs.Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm.esults The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively.Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

Download Full-text

General and Genetic Toxicology of Guayusa Concentrate (Ilex guayusa)

International Journal of Toxicology ◽

10.1177/1091581815625594 ◽

2016 ◽

Vol 35 (2) ◽

pp. 222-242 ◽

Cited By ~ 8

Author(s):

Robert W. Kapp ◽

Odete Mendes ◽

Shambhu Roy ◽

Robert S. McQuate ◽

Richard Kraska

Keyword(s):

Human Lymphocytes ◽

Lethal Dose ◽

Blood Chemistry ◽

Positive Control ◽

Female Rats ◽

Control Group ◽

High Dose ◽

Genetic Toxicology ◽

Male And Female Rats

Tea from the leaves of guayusa ( Ilex guayusa) has a long history of consumption by Ecuadorian natives in regions where the plant is indigenous. The tea contains the methylxanthines caffeine and theobromine as well as chlorogenic acids, flavonoids, and sugars. Various studies were performed to evaluate the general and genetic toxicology of a standardized liquid concentrate of guayusa (GC). Guayusa concentrate was found to be negative in in vitro genotoxicity tests including the Ames test and a chromosome aberration study in human lymphocytes. The oral median lethal dose (LD50) of GC was >5,000 mg/kg for female rats. Guayusa concentrate was administered to male and female rats in a 90-day subchronic study at 1,200, 2,500, and 5,000 mg/kg/d of GC and a caffeine-positive control at 150 mg/kg/d corresponding to the amount of caffeine in the high-dose GC group. Effects observed in the GC-treated groups were comparable to those in the caffeine control group and included reductions in body weights, food efficiency, triglycerides values, and fat pad weights and increases in blood chemistry values for serum aspartate aminotransferase, serum alanine aminotransferase, and cholesterol and adaptive salivary gland hypertrophy. No signs of incremental toxicity due to any other components of guayusa were observed. The studies indicate no harmful effects of GC in these test systems.

Download Full-text

UJI EFEKTIVITAS DAYA ANTHELMINTIK JUS BIJI MENTIMUN (Cucumis Sativum, L) TERHADAP CACING ASCARIDIA GALLI SECARA IN VITRO

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i1.38 ◽

2017 ◽

Vol 2 (1) ◽

pp. 1

Author(s):

Leonov Rianto ◽

Indri Astuti ◽

Ika Prihatiningrum

Keyword(s):

Positive Control ◽

Negative Control ◽

Probit Analysis ◽

Ascaridia Galli ◽

Control Solution ◽

In Vitro Method ◽

Anthelmintic Efficacy ◽

Pyrantel Pamoate ◽

Ld50 Value

Research has been conducted to review the efficacy of anthelmintic on seeing the juice of cucumber seeds ( Cucumis sativum , L ) Against Ascaridia galli by in vitro method . By experimental study was conducted , with samples of seeds of cucumber ( Cucumis sativum , L ) The smoothed and made with 3 concentrations That is 15 % , 30 % and 60 % . The experimental animals used is Ascaridia galli with negative control solution of NaCl 0.9 % and a positive control pyrantel pamoate suspension with concentrations of 0.2 % , 0.4 % and 0.6 % . The analysis of data is probit analysis be adopted . Results Of Research was claimed that the juice of cucumber seeds ( Cucumis sativum , L ) have anthelmintic efficacy against Ascaridia galli with 12.76 % LC50 and LT50 3 hours 4 minutes 39 seconds . Comparising with tables of LD50 value, the obtained dose is 472,59mg / kg and it is in no toxic category.

Download Full-text

In Vitro Comparison of MTA and BC RRM-Fast Set Putty as Retrograde Filling Materials

10.21203/rs.3.rs-1099531/v1 ◽

2021 ◽

Author(s):

Carla Arvelaiz ◽

Andreina Fernandes ◽

Veronica Graterol ◽

Katiuska Gomez ◽

Jose Francisco Gomez-Sosa ◽

...

Keyword(s):

Brain Heart Infusion ◽

Bacterial Count ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Material ◽

Human Teeth ◽

Retrograde Filling ◽

Significant Difference

Abstract Background: To compare, in vitro, the bioceramic materials (MTA and BC RRM-fast set putty) capacity to prevent microleakage of Enterococcus faecalis over time. Methods: An experimental design was made with forty extracted human teeth, coronally cut, and prepared to be placed in a leakage system under sterile conditions. They were randomly divided into an experimental group: thirty teeth (fifteen for retrograde filling material MTA and BC RRM-fast set putty, respectively) and a control group: ten teeth (five positive control, five negative control). The 3 mm root-ends were submerged in a brain-heart infusion broth with a red phenol indicator. The coronal access of each sample was inoculated with E. faecalis every seven days to maintain bacterial viability. The lower chamber was evaluated daily for 30 days to observe the turbidity of the culture medium and establish the presence and day of the filtration. Calculation of the colony-forming units (CFU) was performed for each leaked sample. Results: Of the total samples prepared for each type of bioceramic material, leaked 60.0% (9/15) of the MTA group and 40.0% (6/15) of the BC RRM-fast set putty group. All positive controls filtered on the first day of evaluation, while 20% (1/5) of the negative control leaked in the second week. There was no significant difference in leakage between the bioceramic material types, nor concerning the bacterial count and the type of cement used (p = 0.101). Conclusions: This study suggests that BC RRM-fast set putty and MTA have a similar sealing capacity when used as a retrograde filling material in vitro.

Download Full-text

THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v12i4.10215 ◽

2018 ◽

Vol 12 (4) ◽

Cited By ~ 1

Author(s):

Nuzul Asmilia ◽

T Armansyah TR ◽

Dwinna Aliza ◽

Juli Melia ◽

Erdiansyah Rahmi ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Ethanolic Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Probit Analysis ◽

Antiplasmodial Activity ◽

Control Group ◽

Ic50 Value

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

Download Full-text

Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v1 ◽

2019 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs.Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm.Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively.Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

Download Full-text