scholarly journals Bacterial Cellulose Production in The Overripe Guava Juice by Acetobacter xylinum as A Solution to Reduce Organic Waste

2021 ◽  
Vol 23 (2) ◽  
pp. 41-48
Author(s):  
Annisa Nur Lathifah ◽  
Isa Nuryana
Keyword(s):  
Carbon Source ◽  
Bacterial Cellulose ◽  
Organic Waste ◽  
Sugar Content ◽  
Cost Effective ◽  
Reducing Sugar ◽  
Acetobacter Xylinum ◽  
High Yield ◽  
Guava Fruit ◽  
External Sources

The exploration for a new cost-effective carbon source with shorter fermentation process for high yield BC production is still needed. In this study, bacterial cellulose (BC) was synthesized by Acetobacter xylinum using overripe guava juice as a carbon source. The results showed that A. xylinum was able to grow on the overripe guava juice with different concentration and produced BC after two days incubation. In the later on, the BC is called as nata de guava. The overripe guava juice which containing reducing sugar content 23 g /L (substrate 100%) at pH 4 produced the thickest BC (1.267 cm). This study showed due to the high reducing sugar and protein contents in the overripe guava, without the addition of carbon and nitrogen from external sources, BC could be formed. Considering the huge disposal overripe guava fruit waste in Java, the present study provides an alternative methodology to synthesize BC. Besides, most importantly, this study provides a new insight to manage organic waste specifically from overripe guava fruit rather than the waste being thrown away and becoming organic waste generation. Keywords: bacterial cellulose, Acetobacter xylinum, overripe guava, organic waste

scholarly journals Bacterial cellulose production by Acetobacter xylinum CGMCC 1.2378 using coconut shell acid hydrolysate as carbon source

BioResources ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 1042-1062
Author(s):  
Yu Liu ◽  
Fangfang Wang ◽  
Yangyang Sun
Keyword(s):  
Sulfuric Acid ◽  
Carbon Source ◽  
Bacterial Cellulose ◽  
Sulfuric Acid Concentration ◽  
Potential Method ◽  
Reducing Sugar ◽  
Acetobacter Xylinum ◽  
Coconut Shell ◽  
Acid Hydrolysate ◽  
Hydrolysis Of

Bacterial cellulose (BC) was synthesized by Acetobacter xylinum using a carbon source of coconut shell hydrolysate, which was treated with an ultra-low concentration of sulfuric acid. The coconut shell was found to contain 57.13% holocellulose and 27.42% lignin. The effect of sulfuric acid concentration, reaction temperature, and reaction time on hydrolysis of coconut shell were evaluated by response surface methodology. The reducing sugar concentration was 8.39 g/L under the predicted optimum treatment at 200 °C for 32 min with a solution of 0.07% sulfuric acid. The holocellulose conversion rate was 56.1% at this condition. In a detoxification process using calcium hydroxide and activated carbon, furfural and hydroxymethylfurfural in the hydrolysate were almost completely removed, whereas formic acid and acetic acid levels decreased by 30%. After cultivation for 7 days at the reducing sugar status of 5 g/L, the BC production in medium with the detoxified hydrolysate could reach 1.66 g/L. After fermentation for 21 days, BC yield in medium using composited carbon source (20 g/L) of glucose and hydrolysate was 5.30 g/L. Structural analysis showed that BC obtained from medium of control and detoxified hydrolysate exhibited similar properties. This work provided a potential method for BC production.

scholarly journals PRODUCTION OF BIOETHANOL FROM RICE AND MILLET HUSKS

2021 ◽  
pp. 1-12
Author(s):  
Gwandu AZ ◽  
Farouq AA ◽  
Baki AS ◽  
Peni DN
Keyword(s):  
Weight Loss ◽  
Thermogravimetric Analysis ◽  
Rice Husk ◽  
Sugar Content ◽  
Proximate Analysis ◽  
Reducing Sugar ◽  
High Yield ◽  
Bioethanol Production ◽  
Alternative Source ◽  
Fermentation Period

This study was based on the production of bioethanol as an alternative source of fuel using rice and millet husks. Proximate, elemental and thermogravimetric analysis (TGA) were conducted of the biomass. The proximate analysis revealed that millet husk had the highest moisture content of 26.67±0.58% when compared with rice husk 8.17±0.29%. The TGA of rice husk had a high endset temperature of 355.510C and a weight loss of -48.23%, millet husk had low endset temperature of 349.210C and a weight loss of -44.25%. When thirty grams (30g) of the substrates was used, rice husk revealed the highest reducing sugar content of 2.59±1.24mol/dm3 when compared to millet husk that had 1.63±0.68 mol/dm3. Rice husk indicated low yield after 7 days of fermentation period but showed a significantly high yield in the volume of ethanol produced 68.67±17.69g/l and millet husk had a yield of 79.80±0.93g/l. This indicated that both rice and millet husks were potent for bioethanol production with millet husk having the highest yield.

Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693

2009 ◽  
Vol 76 (2) ◽  
pp. 333-335 ◽  
Author(s):  
Akihiro Kurosumi ◽  
Chizuru Sasaki ◽  
Yuya Yamashita ◽  
Yoshitoshi Nakamura
Keyword(s):  
Carbon Source ◽  
Bacterial Cellulose ◽  
Acetobacter Xylinum ◽  
Fruit Juices

scholarly journals The quality of nata de coco from sawarna and mapanget coconut varieties to the time of storing coconut water

Food Research ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 957-963
Author(s):  
B. Santosa ◽  
W. Wignyanto ◽  
N. Hidayat ◽  
S. Sucipto
Keyword(s):  
Carbon Source ◽  
Design Of Experiments ◽  
Storage Time ◽  
Reducing Sugar ◽  
Acetobacter Xylinum ◽  
Coconut Water ◽  
Palm Fruit ◽  
Random Design ◽  
Main Factor

This study was aimed to find out the type of coconut water and its storage time in order to get the best of nata de coco quality. The design of experiments conducted in this study nested random design 2 factors. The main factor coconut varieties consisting of two levels: palm fruit varieties sawarna and the coconut fruit varieties mapanget, the storage time of coconut water is nested in palm varieties which consists of 5 levels i.e. 0, 6, 12, 18 and 24 hrs. Each treatment was repeated 3 times so that the total of all 30 samples. The results showed that the nata de coco best coconut water obtained from fresh. However, it demonstrated that coconut water stored for 6 hrs of varieties of sawarna, the quality of nata de coco generated no difference from nata de coco coconut water varieties of fresh mapanget (0 hrs of storage) and reducing sugar in coconut water varieties sawarna is an important factor as the carbon source for Acetobacter xylinum. Thus, in order to get nata de coco with the best quality, coconut water of sawarna varieties which is still fresh or has been stored for 6 hrs can be used.

Influence of Date Syrup as a Carbon Source on Bacterial Cellulose Production by Acetobacter xylinum 0416

2016 ◽  
Vol 37 (4) ◽  
pp. 1085-1091 ◽  
Author(s):  
Samaneh Lotfiman ◽  
Dayang Radiah Awang Biak ◽  
Tey Beng Ti ◽  
Suryani Kamarudin ◽  
Saeid Nikbin
Keyword(s):  
Carbon Source ◽  
Bacterial Cellulose ◽  
Acetobacter Xylinum ◽  
Cellulose Production ◽  
Bacterial Cellulose Production ◽  
Date Syrup

Cost-effective upgrading of the Arnsberg WWTP by post denitrification with a moving bed system

2000 ◽  
Vol 41 (9) ◽  
pp. 123-130
Author(s):  
N. Jardin ◽  
L. Rath ◽  
A. Sabin ◽  
F. Schmitt ◽  
D. Thöle ◽  
...  
Keyword(s):  
Carbon Source ◽  
Biological Process ◽  
Cost Benefit Analysis ◽  
Cost Benefit ◽  
Cost Effective ◽  
Benefit Analysis ◽  
Moving Bed ◽  
Trickling Filters ◽  
Sludge Hydrolysis ◽  
Effective Integration

On the basis of a cost-benefit analysis it was decided to expand the Arnsberg WWTP by a multistage biological process which allows for cost-effective integration of the existing facilities. Carbon removal will then be accomplished in a high-loaded activated sludge stage for which the existing primary clarifier is to be reconstructed. The existing trickling filters will be used for nitrification during a midterm period and will be replaced later on either by a moving bed system or by new trickling filters. Line 3 of the existing secondary clarifiers will be reconstructed and used for post denitrification in a moving bed system. The carbon needed for denitrification will be provided by means of sludge hydrolysis and the use of an external carbon source.

Quality Improvement of the DNA extracted by boiling method in Gram negative bacteria

2017 ◽  
Vol 6 (04) ◽  
pp. 5347 ◽  
Author(s):  
Omar B. Ahmed* ◽  
Anas S. Dablool
Keyword(s):  
Dna Extraction ◽  
Control Method ◽  
Extraction Procedure ◽  
Cost Effective ◽  
High Yield ◽  
Gram Negative Bacteria ◽  
Bacterial Dna ◽  
Gram Negative ◽  
E Coli

Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality of the DNA obtained for each one of those methods are variable. The study aimed to evaluate bacterial DNA extraction using conventional boiling method followed by alcohol precipitation. DNA extraction from Gram negative bacilli was extracted and precipitated using boiling method with further precipitation by ethanol. The extraction procedure performed using the boiling method resulted in high DNA yields for both E. coli and K. pneumoniae bacteria in (199.7 and 285.7μg/ml, respectively) which was close to control method (229.3 and 440.3μg/ml). It was concluded that after alcohol precipitation boiling procedure was easy, cost-effective, and applicable for high-yield quality of DNA in Gram-negative bacteria.

scholarly journals Cost Effectiveness of Transbronchial Needle Aspiration

1999 ◽  
Vol 6 (4) ◽  
pp. 332-335 ◽  
Author(s):  
Jennifer A Crocket ◽  
Eric YL Wong ◽  
Dale C Lien ◽  
Khanh Gia Nguyen ◽  
Michelle R Chaput ◽  
...  
Keyword(s):  
Cost Effectiveness ◽  
Cost Effective ◽  
Diagnostic Procedures ◽  
Needle Aspiration ◽  
Benign Disease ◽  
University Hospital ◽  
High Yield ◽  
Transbronchial Needle Aspiration ◽  
Computed Tomographic ◽  
The Cost

OBJECTIVE: To evaluate the yield and cost effectiveness of transbronchial needle aspiration (TBNA) in the assessment of mediastinal and/or hilar lymphadenopathy.DESIGN: Retrospective study.SETTING: A university hospital.POPULATION STUDIED: Ninety-six patients referred for bronchoscopy with computed tomographic evidence of significant mediastinal or hilar adenopathy.RESULTS: Ninety-nine patient records were reviewed. Three patients had two separate bronchoscopy procedures. TBNA was positive in 42 patients (44%) and negative in 54 patients. Of the 42 patients with a positive aspirate, 40 had malignant cytology and two had cells consistent with benign disease. The positive TBNA result altered management in 22 of 40 patients with malignant disease and one of two patients with benign disease, thereby avoiding further diagnostic procedures. The cost of these subsequent procedures was estimated at $27,335. No complications related to TBNA were documented.CONCLUSIONS: TBNA is a high-yield, safe and cost effective procedure for the diagnosis and staging of bronchogenic cancer.

Sign in / Sign up

Export Citation Format

Share Document