scholarly journals DOCKING MOLEKUL DENGAN METODA MOLEGRO VIRTUAL DOCKER DARI EKSTRAK AIR Psidium guajava, Linn DAN Citrus sinensis, Peels SEBAGAI INHIBITOR PADA TIROSINASE UNTUK PEMUTIH KULIT

2013 ◽  
Vol 15 (1) ◽  
pp. 31-39
Author(s):  
Ayik Rosita Puspaningtyas
Keyword(s):  
Amino Acids ◽  
Vitamin C ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Water Extract ◽  
Psidium Guajava ◽  
Positive Control ◽  
Agent Based ◽  
Molegro Virtual Docker ◽  
Guava Fruit

We have been molecular docking using MolegroVirtual Docker (MVD) on water extract of guava fruit(Psidiumguajava, Linn) and sweet orange (Citrussinensis,Peels) as inhibitor on enzyme tyrosinase withascorbic acid (vitamin C) as positive control to studywhitening agent. Based on the previous studies, the maincontent of the water extract of guava fruit (Psidiumguajava)are 2,6-dihydroxy-3,5-dimethyl-4-0--D-glucopyranosilbenzophenone, 3-hydroxy-2-butanone, and vitamin C, whilethe extract juice of sweet oranges (Citrus sinensis) arelimonene, linalol, and vitamin C. In this study the resultsshowed that the main content of the water extract of Psidiumguajava fruit have better bond as inhibitor on tyrosinase thanCitrus sinensis and vitamin C which can be seen fromMoldock score of Psidiumguajava (-107.806) and Citrussinensis (-76,9593); it meanslower the energyand morestable binding. The IC50 on water extract of guava fruit(psidiumguajava) and sweet orange (Citrus sinensis) were0.26 mM and 31.07 mM, respectively. The hydrogen bonds of2, 6-dihydroxy-3, 5-dimethyl-4-0--D- glucopyranosilbenzophenone with 5amino acid of tyrosinase were Gly 200,Pro 201 , Gly 196, Phe 197, and Asn 205, while limonen,linalool binding 3 amino acids were Gly 200, Phe 197, andAsn 205. Finally, 3D MVD visualization between maincontent of guava and sweet orange water extract can beconcluded that interaction of guava fruit (psidiumguajava)water extract against tyrosinase was more harmonious andstabil than vitamin C and main content of sweet orange(Citrus sinensis) water extract.Keywords: Psidium guajava, Citrus sinensis, tyrosinase, vitamin Cand docking molecule

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

Characterization of three terpenoid glycosyltransferase genes in ‘Valencia’ sweet orange (Citrus sinensis L. Osbeck)

Genome ◽  
2010 ◽  
Vol 53 (10) ◽  
pp. 816-823 ◽  
Author(s):  
Jing Fan ◽  
Chunxian Chen ◽  
Qibin Yu ◽  
Zheng-Guo Li ◽  
Frederick G. Gmitter
Keyword(s):  
Amino Acids ◽  
Citrus Sinensis ◽  
Southern Blotting ◽  
Sweet Orange ◽  
Secondary Product ◽  
Reading Frame ◽  
C Terminus ◽  
Group D

Three putative terpenoid UDP-glycosyltransferase (UGT) genes, designated CsUGT1, CsUGT2, and CsUGT3, were isolated and characterized in ‘Valencia’ sweet orange ( Citrus sinensis L. Osbeck). CsUGT1 consisted of 1493 nucleotides with an open reading frame encoding 492 amino acids, CsUGT2 consisted of 1727 nucleotides encoding 504 amino acids, and CsUGT3 consisted of 1705 nucleotides encoding 468 amino acids. CsUGT3 had a 145 bp intron at 730–874, whereas CsUGT1 and CsUGT2 had none. The three deduced glycosyltransferase proteins had a highly conserved plant secondary product glycosyltransferase motif in the C terminus. Phylogenetic analysis showed that CsUGT1 and CsUGT3 were classified into group L of glycosyltransferase family 1, and CsUGT2 was classified into group D. Through Southern blotting analysis, CsUGT1 was found to have two copies in the sweet orange genome, whereas CsUGT2 and CsUGT3 had at least seven and nine copies, respectively. CsUGT1, CsUGT2, and CsUGT3 were constitutively expressed in leaf, flower, and fruit tissues. The results facilitate further investigation of the function of terpenoid glycosyltransferases in citrus and the biosynthesis of terpenoid glycosides in vitro.

scholarly journals Pelilinan Efektif Memperpanjang Masa Simpan Buah Jambu Biji (Psidium guajava L.) ‘Kristal’

2018 ◽  
Vol 9 (1) ◽  
pp. 19-26
Author(s):  
Slamet Susanto ◽  
Delys Inkorisa ◽  
Dadang Hermansyah
Keyword(s):  
Weight Loss ◽  
Vitamin C ◽  
Storage Period ◽  
Titratable Acidity ◽  
Psidium Guajava ◽  
Soluble Solids ◽  
Guava Fruit ◽  
Performance Quality ◽  
Randomized Design ◽  
Solids Content

ABSTRACT‘Kristal’ guava fruit can easily be deteriorated during storage. The objective of this experiment was to study the effect of the waxing on the shelflife and quality of the ‘Kristal’ guava fruit. Experiment was conducted at the Postharvest Laboratory of Departemen of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University from February to May 2016. The experiment was designed using completely randomized design (CRD) with single factor consisted of 5 treatments, i.e., 0% of beewax (control), 2% of beewax, 4% of beewax, 6% of beewax and 8% of beeswax. The results showed that beewax coating suppressed weight loss and softness of fruit compared to the control fruit during storage period. Beewax coating did not significantly affect chemical quality such as soluble solids content, titratable acidity and vitamin C. The beeswax coating with 2% and 4% were able to extend shelflife of ‘Kristal’ guava fruit 9 days longer as compared to the control. Keywords: ‘Krístal’, performance, quality, waxing, weight loss ABSTRAK Buah jambu ‘Kristal’ dapat dengan mudah rusak selama penyimpanan. Tujuan percobaan ini yaitu mempelajari pengaruh pelilinan terhadap daya simpan dan kualitas buah jambu ‘Kristal’. Percobaan dilakukan di Laboratorium Pascapanen, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor dari bulan Februari sampai Mei 2016. Percobaan dirancang menggunakan rancangan acak lengkap (RAL) dengan faktor tunggal terdiri atas 5 perlakuan, yaitu 0% dari lilin lebah (kontrol), 2% dari lilin lebah, 4% lilin lebah, 6% lilin lebah dan 8% lilin lebah. Hasil penelitian menunjukkan bahwa pelapisan lilin lebah menekan susut bobot dan kelunakan buah dibandingkan dengan kontrol selama masa penyimpanan. Pelapisan lilin lebah tidak secara nyata mempengaruhi kualitas kimia buah seperti kandungan padatan terlarut, keasaman titrasi dan vitamin C. Pelapisan lilin lebah dengan konsentrasi 2% dan 4% mampu memperpanjang masa simpan buah jambu ‘Kristal’ 9 hari lebih lama dibandingkan dengan kontrol. Kata Kunci: kualitas, ‘Kristal’, pelilinan, penampilan, susut bobot

scholarly journals UJI EFEKTIVITAS JAMBU BIJI MERAH (Psidium guajava) TERHADAP LAJU ALIRAN SALIVA PADA PENDERITA XEROSTOMIA YANG MENGONSUMSI TELMISARTAN

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Jemima L. Waworuntu ◽  
Jane Wuisan ◽  
Christy N. Mintjelungan
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Flow Rate ◽  
Salivary Flow ◽  
Psidium Guajava ◽  
Salivary Flow Rate ◽  
Control Group ◽  
Control Group Design ◽  
Guava Fruit ◽  
Disposable Syringe

Abstract: A study done by National Center for Biotechnologi Information (NCBI) to the user of antihypertension drugs, 50% of the individuals are suffering xerosomia or dry mouth. The reduction of saliva because of xerostomia may increase the risk of tooth damage. Vitamin C contained in red guava (Psidium Guajava) is expected to induce salivary flow rate in Xerostomia patients who consume antihypertensive Telmisartan.The purpose of this study is to acknowledge the effect of red guava in increasing salivary flow rate in Xerosotmia patients who consume antihypertensive Telmisartan. This is a clinical trial study with nonequivalent control group experimental design. Each group has 15 samples from the population of hypertensive patients who consume antihypertensive Telmisartan and are suffering Xerostomia in Pancaran Kasih Hospital and Prof. Dr. RD. Kandou Hospital. The treatment group is given red guava fruit that is already served as pure juice while the control group is only given mineral water. Saliva is collected twice, that is before treatment and after treatment. Saliva is collected by spitting method and the salivary flow rate is measured by using disposable syringe with the measurement of ml/minute.The result of this study shows that the average of salivary flow rate before of control group is 0.23 ml/minute and the average of salivary flow rate after is 0.28 ml/minute. While the average of salivary flow rate before treatmen in treatment group is 0.24 ml/minute and the average of salivary flow rate after treatment is 0.83 ml/minute. It can be concluded that red guava has been proved to be effectively increase salivary flowrate of xerostomia patients who consume antihypertensive Telmisartan.Keywords: xerostomia, red guava, salivary flow rateAbstrak: Sebuah studi yang dilakukan oleh National Center for Biotechnology Information (NCBI) yang melakukan penelitian terhadap pengguna obat antihipertensi, sebanyak 50% menderita Xerostomia atau mulut kering. Laju aliran saliva yang menurun akibat Xerostomia dapat menyebabkan meningkatnya resiko kerusakan gigi. Kandungan vitamin C pada buah jambu biji merah (Psidium guajava) diharapkan dapat meningkatkan laju aliran saliva pada penderita xerostomia yang mengonsumsi obat antihipertensi golongan Telmisartan.Penelitian ini bertujuan untuk mengetahui efek jambu biji merah dalam meningkatkan laju aliran saliva pada penderita Xerostomia yang mengonsumsi Telmisartan. Penelitian ini adalah penelitian uji klinis dengan rancangan eksperimental nonequivalent control group design. Setiap kelompok beranggotakan 15 orang dari populasi pasien pengguna obat antihipertensi golongan Telmisartan yang menderita Xerostomia di RSU Pancaran Kasih dan RSUP Prof. DR. R. D. Kandou Malalayang. Kelompok perlakuan mengonsumsi buah jambu biji merah yang disajikan dalam bentuk jus murni sedangkan kelompok kontrol hanya mengonsumsi air mineral. Saliva dikumpulkan sebanyak dua kali yaitu sebelum dan sesudah mengonsumsi buah jambu biji merah. Saliva dikumpulkan dengan metode spitting dan laju aliran saliva diukur menggunakan disposable syringe dengan satuan ml/ menit.Hasil penelitian menunjukkan bahwa rerata laju aliran saliva awal pada kelompok kontrol yaitu 0,23 ml/menit dan laju aliran saliva akhir kelompok kontrol yaitu 0,28 ml/menit. Sedangkan rerata laju aliran saliva awal pada kelompok perlakuan yaitu 0,24 ml/menit dan laju aliran saliva akhir kelompok perlakuan yaitu 0,83 ml/menit. Dapat disimpulkan bahwa buah jambu biji merah dapat meningkatkan laju aliran saliva pada penderita Xerostomia yang mengonsumsi Telmisartan.Kata kunci: xerostomia, jambu biji merah, laju aliran saliva.

Guava (Psidium guajava L.) Fruit Coating with Gum – Arabic for Quality and Fruit Fly Control

1970 ◽  
pp. 01-04
Author(s):  
Esameldin B. M. Kabbashi, Ghada H. Abdelrahman and Nawal A. Abdlerahman
Keyword(s):  
Shelf Life ◽  
Gum Arabic ◽  
Fruit Fly ◽  
Psidium Guajava ◽  
Postharvest Quality ◽  
Guava Fruit ◽  
Fruit Shelf Life ◽  
Fly Control ◽  
And Control ◽  
Subtropical Fruit

Guava (Psidium guajava L.) is a lovely tropical and subtropical fruit that originates in Mexico, Central America, and then taken to other distant and near parts around the world. In Sudan this popular fruit is produced in orchards and household and is so profitable but yet attacked by a lot of fruit fly species of the Genera Ceratitis and Bactrocera and the result is a loss of more than 70%. This research aimed at evaluating the effect of Gum Arabic coating (GAC) in extending the shelf life of guava fruit and disinfesting it from these notorious pests. Guava fruits from Kadaro orchards, Khartoum North, were tested using seven concentrations of Gum Arabic solutions. The results reflect that 1: 4 (25%) and 1: 8 (12.5%) (GA: water) concentrations attained 56 and 40% disinfestation, respectively whereas the other lower concentrations effected corresponding results in a range from 20 – 08%. The reduction in maggots per test fruit reached upto 188% as compared to the control.  The highest concentrations (1: 4 & 1: 8) effected a sustainability of 52% in fruit firmness (FF) with an average of medium (3) FF compared to soft FF (4) in the control. The corresponding results in other lower concentrations (1: 16; 1: 32; 1: 64; 1: 72 & 1: 96) were 36, 24, 24, 20 and 16%, respectively. In addition to an average FF of 4 (soft) for all these concentrations and 5 (very soft) for all the corresponding controls. Nevertheless, the sustainability of fruit color (FC) effected by the test concentrations was 52, 44, 24, 22, 24, 20, and 24%, respectively. Regarding these results, the two highest test concentrations effected a sizeable disinfestation and control of fruit flies and a good extension of shelf life of guava in Khartoum State. These findings support using this treatment as an effective IPM tool to extend guava fruit shelf life and upgrading its postharvest quality.

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