COMPOSITION AND CONTENT OF FLAVOINODES OF LEAVES OF HIPPOPHAE RHAMNOIDES L., RELATING IN AZERBAIJAN

Seabuckthorn – Hippophae rhamnoides L. is a valuable food, vitamin and medicinal plant, various parts of which are used to treat diseases as a traditional medicine in many countries of the world. All parts of Hippophae rhamnoides L. are a rich source of biologically active substances, especially polyphenolic compounds, carotenoids, phytosterols and others. Extracts obtained from various sea buckthorn organs possess high antioxidant, antibacterial, antimicrobial, anti-inflammatory, antikanserogenic, antiradiation properties. Analysis of leaves of H. rhamnoides on the content of flavonoids showed that they contain from 2.81 to 3.2% flavonoids. Chromatographic spectrophotometric methods were used to study the qualitative composition and content of the leaf flavonoids of male H. rhamnoides. Five individual flavonoids were isolated from the sum of flavonoids, the isolated flavonoids identified as quercetin, mirisetin, isoramnetin, quercetin-3-rutinoside (rutin) and isoramnetin-3-rutinoside (narcissine), on the basis of chromatographic data, UV spectra, and acid hydrolyses. Routine and narcissine are the main components of leaves of H. rhamnoides. It has been established that the content of flavonoids is significant, with the main components being biologically active flavonoids such as rutin and narcissine, thus the leaves of the male sea buckthorn are a promising source of raw materials for the production of P vitamins and food additives.

Download Full-text

Stability of Carotene and Phenols of Sea Buckthorn (Hippophae rhamnoides L.) Juice with Pomace during Storage

Periodica Polytechnica Chemical Engineering ◽

10.3311/ppch.15641 ◽

2020 ◽

Author(s):

Chagnaadorj Rentsendavaa ◽

Dóra Székély ◽

Diána Furulyás ◽

György Végvári ◽

Faraja Gonelimali ◽

...

Keyword(s):

Antioxidant Capacity ◽

Sea Buckthorn ◽

Biologically Active ◽

Hippophae Rhamnoides ◽

Food Ingredient ◽

Carotene Content ◽

Total Period ◽

Hippophaë Rhamnoides ◽

Hippophae Rhamnoïdes L ◽

Β Carotene

Sea Buckthorn (SB) juice is rich in biologically active compounds. It has considerable health benefits; thus, it can serve as functional food ingredient. The aim of this study was to determine and compare the total content of polyphenols, antioxidant capacity (FRAP), soluble solid content, pH value, β-carotene content and colour parameters (L*, a*, b*) in berry juice of cultivar 'Leikora' Sea Buckthorn (SB) (Hippophae rhamnoides L.). The treatment was made as control sample of Sea Buckthorn juice (C), and Sea Buckthorn juice with 0.5 % (P0.5); 1 % (P1); 2 % (P2) dried pomace of Sea Buckthorn. The samples were stored at room temperature for physicochemical analysis at interval of 2 months for a total period of 14 months.According to our results, the β-carotene (C to P2 was 3.71, 4.82, 5.49 and 6.52 mg 100mL−1) as well as the antioxidant capacity of the samples increased with the growth of the pomace content. During storage, degradation occurred in the polyphenol content and antioxidant capacity, but the β-carotene content increased. The increase of the β-carotene content was 94.6 % (C) and 32.7 % (P0.5). The smaller reduction in antioxidant compounds was the higher the sample of pomace content is. The FRAP and total polyphenol values measured during storage confirm that the pomace has antioxidant effect. There is a close correlation between the two parameters, including a positive correlation (r = 0.8614), which indicates that a significant part of the antioxidant capacity of buckthorn is due to the presence of different polyphenols.

Download Full-text

Chemical composition of sea buckthorn (Hippophae rhamnoides L.) berry grown in the Chelyabinsk region

Vestnik MGTU ◽

10.21443/1560-9278-2021-24-3-306-312 ◽

2021 ◽

Vol 24 (3) ◽

pp. 306-312

Author(s):

N. L. Naumova

Keyword(s):

Chemical Composition ◽

Mineral Elements ◽

Sea Buckthorn ◽

Biologically Active ◽

Hippophae Rhamnoides ◽

Customs Union ◽

Hippophaë Rhamnoides ◽

Hippophae Rhamnoïdes L ◽

Chelyabinsk Region ◽

Fat Soluble Vitamins

The most important phytonutrients of sea buckthorn ( Hippophae rhamnoides L.) berry are carotenoids, flavonoids, anthocyanins, sugars, organic and amino acids, water and fat-soluble vitamins, tannins and pectin substances, phospholipids, macro- and microelements. In the Chelyabinsk region it is almost impossible to find well-groomed industrial plantings of sea buckthorn, since its main areas (600 hectares) are concentrated in amateur and home gardens. To form a healthy diet, it is important not only to ensure the safety of horticultural products, but also to raise the priority of signs that determine consumer demand, namely the quality and chemical composition of fruits, their saturation with the necessary micronutrients. While studying food and biologically active substances of sea buckthorn berries of the "Velikan" variety growing in different garden agrocenoses of the Chelyabinsk region it has been determined that sea buckthorn berries cultivated in the garden "Lokomotiv-1" (Chelyabinsk) contain more flavonoids (by 21.4 %), proteins (by 17.4 %), mineral elements - Al (in 5.3 times), V (3.4 times), Cu (3 times), Ti (2.4 times), Ca and Fe (1.9 times), Mn (1.5 times), in berries obtained in the garden "Dizelist-1" (Chelyabinsk region, Troitsk) contains more sugars (by 15.1 %), as well as Mo and Sr (2-2.3 times), Se (1.4 times), Cr (by 76.2 %), etc. In terms of Se content, the studied fruits exceed the clarke values by 4-6 times; in terms of Pb content, they turned out to be as close as possible to the upper limit of MPC according to the requirements of the Technical Regulations of the Customs Union. The study of the mineral composition of sea buckthorn is especially important in the context of the ecological disadvantage of the Chelyabinsk region due to the pollution of the environment with heavy metals.

Download Full-text

Morphological and Genetic Diversity of Sea Buckthorn (Hippophae rhamnoides L.) in the Karakoram Mountains of Northern Pakistan

Diversity ◽

10.3390/d10030076 ◽

2018 ◽

Vol 10 (3) ◽

pp. 76 ◽

Cited By ~ 2

Author(s):

Muhammad Nawaz ◽

Konstantin Krutovsky ◽

Markus Mueller ◽

Oliver Gailing ◽

Asif Khan ◽

...

Keyword(s):

Genetic Diversity ◽

International Markets ◽

Sea Buckthorn ◽

Hippophae Rhamnoides ◽

Morphometric Traits ◽

Hippophaë Rhamnoides ◽

Hippophae Rhamnoïdes L ◽

Karakoram Mountains ◽

Gilgit Baltistan ◽

Promising Source

Sea buckthorn (Hippophae rhamnoides L.) is a dioecious, wind-pollinated shrub growing in Eurasia including the Karakoram Mountains of Pakistan (Gilgit-Baltistan territory). Contrary to the situation in other countries, in Pakistan this species is heavily underutilized. Moreover, a striking diversity of berry colors and shapes in Pakistan raises the question: which varieties might be more suitable for different national and international markets? Therefore, both morphological and genetic diversity of sea buckthorn were studied to characterize and evaluate the present variability, including hypothetically ongoing domestication processes. Overall, 300 sea buckthorn individuals were sampled from eight different populations and classified as wild and supposedly domesticated stands. Dendrometric, fruit and leaf morphometric traits were recorded. Twelve EST-SSRs (expressed sequence tags-simple sequence repeats) markers were used for genotyping. Significant differences in morphological traits were found across populations and between wild and village stands. A significant correlation was found between leaf area and altitude. Twenty-two color shades of berries and 20 dorsal and 15 ventral color shades of leaves were distinguished. Mean genetic diversity was comparatively high (He = 0.699). In total, three distinct genetic clusters were observed that corresponded to the populations’ geographic locations. Considering high allelic richness and genetic diversity, the Gilgit-Baltistan territory seems to be a promising source for selection of improved germplasm in sea buckthorn.

Download Full-text

Research on the acute toxicity of biologically active compounds derived from Sea buckthorn (Hippophae rhamnoides L. ) flakes in vivo

Bulletin of Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences ◽

10.5564/bicct.v0i7.1270 ◽

2019 ◽

pp. 28-32

Author(s):

Munkhtsetseg B ◽

Mungunnaran D ◽

Odonchimeg M ◽

Bayarmaa B

Keyword(s):

Heavy Metals ◽

Acute Toxicity ◽

Biologically Active Compounds ◽

Sea Buckthorn ◽

Biologically Active ◽

Hippophae Rhamnoides ◽

Total Flavonoids ◽

Active Compounds ◽

Hippophaë Rhamnoides ◽

Hippophae Rhamnoïdes L

Recently, plants, animal and compounds by minerals are increased to use trend. Raw materials from plant, especially sea buckthorn, have the high contents of biologically active compounds, then, these compounds demonstrate some special properties for medicine and functional that including to supply deficiency of minerals and vitamins, to improve immune system, metabolism, and tissue culture, to anti-inflammation, to detoxification, to immune human body. In our previous study, we determined optimal conditions for extracting total flavonoids from sea buckthorn (Hippophae rhamnoides L.) fruit flakes and purified biologically active compounds in laboratory conditions. Level of total flavonoids, the main active ingredient extracted from sea buckthorn fruit flakes, heavy metals, and the hygiene parameters were determined in the current study. Total flavonoids were 2.1%, heavy metals, including cadmium and lead, did not exceed maximum permissible levels, and there were no bacteria or fungi detected.The acute toxicity study performed on the C57/BL6 strain of mice by administering 500-6000 mg/kg of extract orally. The LD50 was more than 6000 mg/kg, which is classified as a non-toxic compound based on Hodge and Sterner toxicity scale. Чацаргана (Hippophae rhamnoides L.) жимсний хальснаас гарган авсан биологийн идэвхт бэлдмэлийн хорон чанарын судалгаа Хураангуй: Орчин үед ургамал, амьтан, эрдсийн гаралтай бэлдмэлүүдийг хэрэглэх хандлага өсөн нэмэгдэж байна. Ургамлын гаралтай түүхий эдүүд ялангуяа чацаргана жимс нь биологийн идэвхт олон бодисуудыг өндөр тунгаар агуулдаг бөгөөд бие махбодийн эрдэс бодис, витамины дутагдлыг нөхөх, дархлааг сайжруулах, үрэвслийн эсрэг үйлдэл үзүүлэх, бодисын солилцоог эрчимжүүлэх, эд эсийн төлжилтийг сайжруулах, хоргүйжүүлэх зэрэг эмчилгээний болон зохицуулах үйлчилгээтэй өвөрмөц шинжийг үзүүлдэг.Бид өмнөх судалгаагаар чацаргана жимсний хальснаас нийлбэр флавоноидыг хандлах, тохиромжтой горимыг тогтоож, биологийн, идэвхт бэлдмэлийг лабораторийн нөхцөлд гарган авсан. Чацаргана (Hippophae rhamnoides L.) жимсний хальснаас гарган авсан бэлдмэлийн гол үйлчлэгч бодис болох нийлбэр флавоноидын агууламж, эрүүл ахуй, хүнд металлын үзүүлэлтүүдийг тодорхойлоход флавоноидын агууламж нь 2.1%, кадми, хар тугалгын агууламж хүнсний бүтээгдэхүүнд зөвшөөрөгдөх дээд хэмжээнээс бага, бактери, хөгц мөөгөнцөр илрээгүй болно.Бэлдмэлийн хорон чанарын туршилтыг C57/BL6 үүлдрийн шугаман хар хулгана дээр хийсэн бөгөөд бэлдмэлийг 500-6000 мг/кг тунгаар хулганад амаар олгоход LD50 нь 6000 мг/кг-аас дээш хэмжээтэй байгаа нь Ходжа ба Стернерийн хорон чанарын ангилалаар хоргүй ангилалд багтаж байна. Түлхүүр үг: чацарганы хальс, флавоноид, бэлдмэл, хорон чанар .

Download Full-text

Protective Effects of Sea Buckthorn (Hippophae rhamnoides L.) Leaves Fermented with Hericium erinaceum Mycelium against Oxidative Modification of Biological Macromolecules and Cell Death

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2015.44.1.035 ◽

2015 ◽

Vol 44 (1) ◽

pp. 35-43

Author(s):

Seung-Sub Kim ◽

Inn-Goo Kyeong ◽

Mi-La Lee ◽

Dong-Goo Kim ◽

Ji-Young Shin ◽

...

Keyword(s):

Cell Death ◽

Oxidative Modification ◽

Sea Buckthorn ◽

Hippophae Rhamnoides ◽

Biological Macromolecules ◽

Protective Effects ◽

Hippophaë Rhamnoides ◽

Hericium Erinaceum ◽

Hippophae Rhamnoïdes L

Download Full-text

Effect of Ozone Treatment on the Quality of Sea Buckthorn (Hippophae rhamnoides L.)

Plants ◽

10.3390/plants10050847 ◽

2021 ◽

Vol 10 (5) ◽

pp. 847

Author(s):

Anita Zapałowska ◽

Natalia Matłok ◽

Miłosz Zardzewiały ◽

Tomasz Piechowiak ◽

Maciej Balawejder

Keyword(s):

Mechanical Properties ◽

Volatile Compounds ◽

Sea Buckthorn ◽

Hippophae Rhamnoides ◽

Ozone Treatment ◽

Aerobic Bacteria ◽

Hippophaë Rhamnoides ◽

Hippophae Rhamnoïdes L ◽

Ozonation Process

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.

Download Full-text