Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

The objective was to evaluate the use of cryogenic solutions in cryopreservation Sinningia leucotricha seeds by the vitrification method in liquid nitrogen. The treatments were: T1 - control: without cryoprotective solution; T2 - PVS1; T3 - modified PVS1; T4 - PVS2; T5 - modified PVS2; T6 - PVS3; T7 - PVS3 modified; T8 - PVS2 + 1% phloroglucinol. After 15 days of immersion of seeds in LN, the cryotubes were removed and rapidly reheated to a temperature of 40 °C water bath for 1.5 minutes. Then, the seeds were washed with wash solution for 20 min. Later they were submitted to the germination test which was conducted on blotting paper moistened with distilled water packaged in crystal polystyrene boxes kept in a growth chamber at 25 ± 2 °C and 16 hours photoperiod. Was evaluated the germination percentage the germination speed index (GSI), and at the end of the experiment will determine the length of shoot (LS) and root (LR), and dry mass of seedlings (DMS). We used a completely randomized design with eight treatments and five replications, consisting of 100 seeds. The direct submission of the queen of the abyss seeds in liquid nitrogen provided the highest germination values, GSI, LS, LR and DMS. The queen of the abyss seeds can be cryopreserved in liquid nitrogen directly without the need to cryoprotectant solutions.

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Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

Acta Scientiarum Biological Sciences ◽

10.4025/actascibiolsci.v41i1.46030 ◽

2019 ◽

Vol 41 ◽

pp. e46030

Author(s):

João Everthon da Silva Ribeiro ◽

Arliston Pereira Leite ◽

Jackson Silva Nóbrega ◽

Edna Ursulino Alves ◽

Riselane de Lucena Alcântara Bruno ◽

...

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Seed Vigor ◽

Randomized Design ◽

Temperature Regimes ◽

Seedling Length ◽

Physiological Quality ◽

Mean Germination Time ◽

Completely Randomized Design ◽

Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

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Effects of aqueous plant extracts and inorganic fertilizer on the germination, growth and development of maize (Zea mays L.)

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas1403243c ◽

2014 ◽

Vol 59 (3) ◽

pp. 243-254 ◽

Cited By ~ 1

Author(s):

Kanayo Chukwuka ◽

Maxwell Obiakara ◽

Israel Ogunsumi

Keyword(s):

Plant Extracts ◽

Growth And Development ◽

Tap Water ◽

Germination Percentage ◽

Distilled Water ◽

Aqueous Extracts ◽

Radicle Growth ◽

Randomized Design ◽

Npk Fertilizer ◽

Completely Randomized Design

Screen house experiments were carried out to examine the effects of aqueous leaf extracts of Tithonia diversifolia (Ti) and Vernonia amygdalina (Ve), as well as NPK fertilizer (15-15-15) on the germination, growth and development of maize. Fresh leaves were collected, washed with tap water, chopped and pounded, soaked in distilled water and filtered. The two filtrates were used to prepare extracts at 50 and 100% w/v. Four maize seeds were placed in Petri dishes laid out in a completely randomized design with five replicates. In every Petri dish 10 ml of extract per treatment was added. A control experiment with distilled water was also set up. Also, in a completely randomized design with four replicates, 4-week-old potted maize plants were treated with 500 ml of each extract as well as with 1.52 g of NPK fertilizer. The results showed that the germination percentage of the seeds followed the order Ti50 - Control - Ve50. The seeds treated with aqueous extracts of T. diversifolia and V. amygdalina at 100% w/v produced lower but equal germination percentage. The seedling radicle growth was significantly inhibited by the aqueous extracts of Ti100, Ve50 and Ve100 (p?0.05). The inhibition was dose-dependent and more pronounced in seeds treated with extracts of V. amygdalina. The aqueous extract of T. diversifolia (50% w/v) and control influenced radicle growth substantially. All the extracts inhibited the plumule development compared to the control. On the other hand, growth, development and yield were not significantly affected by the plant extracts.

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Physiological potential of piquin pepper seeds in response to pregermination treatments

Ciência e Agrotecnologia ◽

10.1590/1413-7054202145019521 ◽

2021 ◽

Vol 45 ◽

Author(s):

Miguel Ángel Cano-González ◽

Benjamín Abraham Ayil-Gutiérrez ◽

Rafael Delgado-Martínez ◽

Eduardo Osorio-Hernández ◽

José Antonio Rangel-Lucio ◽

...

Keyword(s):

Acetic Acid ◽

Salicylic Acid ◽

Growth Regulators ◽

Germination Percentage ◽

Wild Plant ◽

Naphthalene Acetic Acid ◽

Distilled Water ◽

Randomized Design ◽

Completely Randomized Design ◽

Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

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Physiological maturation of cowpea seeds

Journal of Seed Science ◽

10.1590/2317-1545v36n31007 ◽

2014 ◽

Vol 36 (3) ◽

pp. 312-317 ◽

Cited By ~ 3

Author(s):

Narjara Walessa Nogueira ◽

Rômulo Magno Oliveira de Freitas ◽

Salvador Barros Torres ◽

Caio César Pereira Leal

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Maturation Process ◽

Cowpea Seeds ◽

Randomized Design ◽

Germination Process ◽

Physiological Quality ◽

Completely Randomized Design ◽

Nutrient Reserves

The seed maturation process is genetically controlled and involves an arranged sequence of morphological and physiological changes extending from fertilization to its total independence from the mother-plant. These changes also include a set of preparatory phases for the germination process, which are characterized for the synthesis and accumulation of nutrient reserves. Thereby, this study was developed aiming at assessing development and physiological quality of cowpea seeds during maturation process. To this, the cowpea pods of cultivar BRS-Guariba were harvested from the tenth day after anthesis (DAA) until the twenty sixth DAA, with four days intervals. Immediately after each harvest, seeds were manually extracted from the pods and then subjected to the following determinations: moisture content, first count of germination, final germination percentage, length of shoots and roots, hypocotyl diameter, and seedling dry mass. The experiment was conducted in a completely randomized design, with five treatments (DAA), and four replications to each treatment. Results have shown that cowpea seeds have fairly fast physiological maturation, and that seeds harvested between 14 and 18 DAA have better vigor as well as higher germination rates; thus the harvest performed during this period does not cause damages to seeds.

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CARPOSEMINAL BIOMETRY AND GERMINATION OF Cabralea canjerana (Vell.) Mart.

FLORESTA ◽

10.5380/rf.v47i4.54128 ◽

2017 ◽

Vol 47 (4) ◽

pp. 391 ◽

Cited By ~ 1

Author(s):

Helena Cristina Rickli-Horst ◽

Manoela Mendes Duarte ◽

Cacilda João Chirinzane ◽

Antonio Carlos Nogueira

Keyword(s):

Native Species ◽

Germination Rate ◽

Potential Temperature ◽

Dry Mass ◽

Germination Percentage ◽

Germination Test ◽

Mean Values ◽

Blotting Paper ◽

Mean Germination Time ◽

Digital Caliper

Cabralea canjerana is a species that has ecological and economic importance for the Southern Brazil. This study aimed to characterize the biometry of fruits and seeds as well as the C. canjerana germinative process submitted to different substrates and temperatures. The biometric characteristics of fruits and seeds were analyzed with the aid of digital caliper. In the germination test, the substrates blotting paper, sand and vermiculite, and the temperatures of 20, 25 and 30 ºC were used. The germination percentage, mean germination rate (MGR), mean germination time (MGT), synchronization index (SI), fresh mass (FS) and dry mass (DM) of seedlings were assessed. The highest germination percentages occurred for vermiculite at three temperatures, blotting paper at 25 °C and sand at 20 and 30 °C. There was seedling formation in all treatments. The use of the sand substrate and the temperatures of 25 and 30 °C provided lower mean values of MGT and SI and higher mean values of MGR. The highest DM occurred with sand at the temperature of 20 ºC. The biometric characteristics of fruits and seeds varied. We recommend the use of vermiculite at temperatures of 20, 25 and 30 ºC, blotting paper at 25 °C and sand at 20 and 30 °C for germination tests.Keywords: Meliaceae, native species, germination potential, temperature, substrate. Resumo:Biometria carpo seminal e germinação de Cabralea canjerana (Vell.) Mart. Cabralea canjerana é uma espécie que possui importância ecológica e econômica para o Sul do Brasil. Este estudo objetivou caracterizar a biometria de frutos e sementes, assim como o processo germinativo de C. canjerana submetido a diferentes substratos e temperaturas. Analisaram-se as características biométricas de frutos e sementes com auxílio de paquímetro digital. No teste de germinação, foram utilizados os substratos papel mata-borrão, areia e vermiculita e as temperaturas de 20, 25 e 30 ºC, avaliando-se a porcentagem de germinação, velocidade média de germinação (VMG), tempo médio de germinação (TMG), índice de sincronização (I), massa fresca (MF) e massa seca (MS) de plântulas. As maiores porcentagens de germinação ocorreram com vermiculita nas três temperaturas, papel mata-borrão a 25 °C e areia a 20 e 30 °C. Houve formação de plântulas em todos os tratamentos. O uso do substrato areia e das temperaturas de 25 e 30 ºC proporcionou menores médias de TMG e I e maiores médias de VMG. A maior MS ocorreu utilizando-se areia sob temperatura de 20 ºC. Ocorreu variação das características biométricas de frutos e sementes. Recomenda-se a utilização de vermiculita nas temperaturas de 20, 25 e 30 ºC, papel mata-borrão a 25 °C e areia a 20 e 30 °C para testes de germinação.Palavras-chave: Meliaceae, espécie nativa, potencial germinativo, temperatura, substrato.

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Evidence of maternal effect on germination and vigor of sour passion fruit

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452020002 ◽

2020 ◽

Vol 42 (4) ◽

Author(s):

Luciana Domiciano Silva Rosado ◽

João Paulo Gava Cremasco ◽

Carlos Eduardo Magalhães dos Santos ◽

Claudio Horst Bruckner ◽

Maria Helena Menezes Cordeiro ◽

...

Keyword(s):

Maternal Effect ◽

Dry Mass ◽

Germination Percentage ◽

Passion Fruit ◽

Reciprocal Effects ◽

Passiflora Edulis ◽

Randomized Design ◽

High Germination ◽

Completely Randomized Design ◽

Sour Passion Fruit

Abstract The seminiferous propagation of Sour Passion Fruit (Passiflora edulis Sims) is the main kind of multiplication due to the ease formation of seedlings. However, the reduced number of productive and homogeneous cultivars reflects the need for genetic breeding. The objective of this study was to assess the maternal and reciprocal effects in vigor and germination of Sour Passion Fruit seeds. The experiment was conducted in a completely randomized design in the factorial scheme, with two structure (hybrid and reciprocal) and ten crosses with four replicates (50 seeds) in trays containing inert sand in germination chamber. Before sowing, it was obtained the weight of 100 seeds, and at 28 days germination percentage, emergence speed index, total seedlings length, shoot length and main root length, number of normal seedlings and dry mass per seedlings were evaluated. The reciprocal effects tell us which genotype is most promising when used as the female or male parent for the trait studied. It can be inferred that there is maternal effect influencing the weight of 100 seeds, emergence, emergence speed index and the number of normal seedlings. There was influence of the parent on the expression of traits in seeds of Sour Passion Fruit. It is important to define the paternal and maternal parent to obtain seeds with high germination potential.

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The Effect of Dormancy Breakdown through Water Immersion with Temperature Stratification on the Germination of Sugar Palm Seeds (Arenga pinnata)

Jurnal Sylva Lestari ◽

10.23960/jsl1877-84 ◽

2020 ◽

Vol 8 (1) ◽

pp. 77

Author(s):

Astry Sri Rezeki Rumahorbo ◽

Duryat . ◽

Afif Bintoro

Keyword(s):

Germination Rate ◽

Water Immersion ◽

Germination Percentage ◽

Germination Test ◽

Physical Dormancy ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

C 50 ◽

Control Water

Sugar palm (Arenga pinnata) has a hard and impermeable pericarp, which make an obstacle for it’s germination. Scarification of seed is needed to breakdown the physical dormancy of seeds. One of the methods to break the physical dormancy is stratification by soaking seed in to the water in some different temperature level. The aims of the study were to determine the effectiveness of stratification and to figure out the best water temperature in breaking the dormancy. A Completely Randomized Design (CRD) in four treatments (control, water immersion at 25°C, 50°C, and 75°C) with four replications was employed as research design. Statistical analysis was conducted using homogenity of variance, variance analysis, and least significant difference tests. The result showed that the water immersion of arenga seed at 25°C and 50°C resulted in the germination percentage, germination rate and test germination that was comparable to control. Water immersion at 75°C reduced the percentage of germination and germination rate, however the treatment could increase the germination test. Water immersion of arenga seed at 25°C, 50°C, and 75°C were not effective to break the dormancy of sugar palm seed.Keywords: aren seeds, dormancy, water immersion

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TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

Revista Caatinga ◽

10.1590/1983-21252018v31n403rc ◽

2018 ◽

Vol 31 (4) ◽

pp. 817-822 ◽

Cited By ~ 2

Author(s):

Antônia Adailha Torres Souza ◽

Narjara Walessa Nogueira ◽

Salvador Barros Torres ◽

Clarisse Pereira Benedito ◽

Maria Lilia de Souza Neta ◽

...

Keyword(s):

Test Procedure ◽

Seed Viability ◽

Abelmoschus Esculentus ◽

Tetrazolium Salt ◽

Distilled Water ◽

Germination Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Tetrazolium Test

ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

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Competitiveness of alexandergrass or Bengal dayflower with soybean

Planta Daninha ◽

10.1590/s0100-83582010000300008 ◽

2010 ◽

Vol 28 (3) ◽

pp. 515-522 ◽

Cited By ~ 7

Author(s):

A.C.R. Dias ◽

S.J.P. Carvalho ◽

L.W. Marcolini ◽

M.S.C. Melo ◽

P.J. Christoffoleti

Keyword(s):

Competitive Ability ◽

Dry Mass ◽

Total Density ◽

Weed Species ◽

Replacement Series ◽

Commelina Benghalensis ◽

Randomized Design ◽

Full Flowering ◽

Completely Randomized Design ◽

Soybean Plants

Weeds compete with field crops mainly for water, light and nutrients, and the degree of competition is affected by the weed density and the intrinsic competitive ability of each plant species in coexistence. The objective of this research was to compare the competitiveness of alexandergrass (Brachiaria plantaginea) or Bengal dayflower (Commelina benghalensis) in coexistence with soybean, cv. M-Soy 8045. A factorial experiment (2 x 5) with two weed species and five competition proportions was carried out in a completely randomized design with four replicates. Proportions were based on a replacement series competition design, always maintaining the total density of four plants per 10 L plastic pots, which corresponded to 60 plants m ². The weed-crop proportions were: 0:4; 1:3; 2:2; 3:1; 4:0; that corresponded to the proportion of 100, 75, 50, 25 and 0% of soybean plants and the opposite for weeds, B. plantaginea or C. benghalensis plants. Leaf area, shoot dry mass of the weeds and soybean and number of soybean trifoliate leaves were evaluated when the soybean reached the phenologic stage of full flowering. B. plantaginea was a better competitor than soybean plants. Otherwise, C. benghalensis revealed a similar competitive ability that of the soybean. In both cases, there were evidences that intraspecific competition was more important.

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INFLUENCE OF SUGARCANE STRAW AND SOWING DEPTH ON THE EMERGENCE OF WEED SPECIES

Planta Daninha ◽

10.1590/s0100-83582015000300003 ◽

2015 ◽

Vol 33 (3) ◽

pp. 405-412 ◽

Cited By ~ 2

Author(s):

P. V. SILVA ◽

P. A. MONQUERO ◽

F. B. SILVA ◽

N. C. BEVILAQUA ◽

M. R. MALARDO

Keyword(s):

Ricinus Communis ◽

Seedling Emergence ◽

Dry Mass ◽

Weed Species ◽

Sowing Depth ◽

Sugarcane Straw ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Luffa Aegyptiaca

ABSTRACT This study aimed to understand the influence of sowing depth and the amount of sugarcane straw on the emergence of weed species Luffa aegyptiaca Miller (Cucurbitaceae); Mucuna aterrima Piper & Tracy (Fabaceae - Leguminosae) and Ricinus communis (Euphorbiaceae). A completely randomized design with a 5 x 4 x 3 factorial layout with four replications was used, at five sowing depths (0, 2, 4, 8 and 10 cm), four different amounts of sugarcane straw (0, 5, 10 and 15 t ha-1) and three different evaluation periods (7, 14 and 21 days after sowing). After sowing, different amounts of sugarcane straw (0, 5, 10 and 15 t ha-1) were deposited on soil. Seedling emergence was analyzed at 7, 14 and 21 days after sowing, counting the number of seedlings that had emerged. At the end of the trial, weed height (cm), leaf area (cm2) and shoot dry mass (g) were measured. In relation to emergence ability, studied species presented different responses according to sowing depth and to the amount of sugarcane straw deposited on the soil. For the L.aegyptiacaand M.aterrima, no significant difference was observed in the interaction between depth and sugarcane straw, showing the adaptation of these species to no-burn sugarcane system. For R.communis, seeds placed at 0 cm of sugar cane straw depth were observed to favor the emergence of seedlings.

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