scholarly journals The Effect of Dormancy Breakdown through Water Immersion with Temperature Stratification on the Germination of Sugar Palm Seeds (Arenga pinnata)

2020 ◽  
Vol 8 (1) ◽  
pp. 77
Author(s):  
Astry Sri Rezeki Rumahorbo ◽  
Duryat . ◽  
Afif Bintoro
Keyword(s):  
Germination Rate ◽  
Water Immersion ◽  
Germination Percentage ◽  
Germination Test ◽  
Physical Dormancy ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
C 50 ◽  
Control Water

Sugar palm (Arenga pinnata) has a hard and impermeable pericarp, which make an obstacle for it’s germination. Scarification of seed is needed to breakdown the physical dormancy of seeds. One of the methods to break the physical dormancy is stratification by soaking seed in to the water in some different temperature level.  The aims of the study were to determine the effectiveness of stratification and to figure out the best water temperature in breaking the dormancy. A Completely Randomized Design (CRD) in four treatments (control, water immersion at 25°C, 50°C, and 75°C) with four replications was employed as research design.  Statistical analysis was conducted using homogenity of variance, variance analysis, and least significant difference tests. The result showed that the water immersion of arenga seed at 25°C and 50°C resulted in the germination percentage, germination rate and test germination that was comparable to control. Water immersion at 75°C reduced the percentage of germination and germination rate, however the treatment could increase the germination test. Water immersion of arenga seed at 25°C, 50°C, and 75°C were not effective to break the dormancy of sugar palm seed.Keywords: aren seeds, dormancy, water immersion

scholarly journals Estimation of the cardinal temperatures for germination of four Satureja species growing in Iran

2016 ◽  
Vol 62 (1) ◽  
pp. 7-21 ◽  
Author(s):  
Hamed Zarif Ketabi ◽  
Hamid Reza Khazaei ◽  
Ahmad Nezami ◽  
Seyed Reza Tabaei Aghdaei
Keyword(s):  
Regression Models ◽  
Germination Rate ◽  
Germination Percentage ◽  
Randomized Design ◽  
Significant Difference ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Genetic And Environmental Factors ◽  
Cardinal Temperatures ◽  
Temperature Water

SummaryIntroduction Seed germination is a complex physiological process regulated by genetic and environmental factors including temperature, water, oxygen, light and pH. Among them, temperature is one of the most important factors controlling the maximum rate and percentage of diaspore germination. Objective: The aim of the study was to determine the cardinal temperatures (Tb, To, Tc) of four Satureja species growing in Iran. Methods: Seeds of Satureja mutica Fish. et C. A. Mey., S. macrantha C. A. Mey., S. sahandica Bornm and S. bachtiarica Bunge were germinated at nine constant temperatures (from 0 to 40°C) with 5°C intervals. A factorial experiment based on completely randomized design with four replications was applied to determine the cardinal temperatures estimated by three regression models including intersected-lines (ISL), quadratic polynomial (QPN) and five-parameters beta (FPB). Results: The highest germination percentage (GP) occurred at 20°C for S. mutica (86%), S. macrantha (55%), S. sahandica (81%) and S. bachtiarica (89%), but there was no significant difference between 20 and 25°C, except S. sahandica. Germination stopped at 0°C and 40°C. The highest germination rate (GR), the lowest mean germination time (MGT) and time to 50% germination (D50) were obtained at 20-25°C for all species. The GRmfor S. bachtiarica was significantly (p≤0.05) higher than for three other species in all temperatures. None of the species did reach to 50% germination at temperatures higher than 30°C. Conclusion: Obtained results revealed the superiority of S. bachtiarica over the other species, v.s. S. macrantha was inferior. FPB and ISL models were most reliable for predicting cardinal temperatures, because of higher R2value and the lower root mean square error (RMSE). S. macrantha and S. mutica showed the lowest and the highest cardinal temperatures, respectively, in all three models.

scholarly journals Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of Deteriorated Soybean Seed

2018 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
P.K. Dewi Hayati
Keyword(s):  
Seed Viability ◽  
Soybean Seed ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Time Data ◽  
Randomized Design ◽  
Vigor Index ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Hydration And Dehydration

Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integrated with rhizobateri to viability and vigor of deteriorated soybean seed.  A completely randomized design with four replicates were used in this experiment. Seeds were treated with varied combination of hydration period in a rhizobacteria suspension and followed by dehydration time.  Data were analysed using the F-test and significant differences were further tested with Least Significant Difference at the 5% level. Results showed that the hydration and dehydration period of seeds in a rhizobacteria suspension improved viability and vigor of seed which have 59.5% initial germination percentage. The improvement was 12.28%, 0.56, 25.4% and 1.4 days for standard germination test, vigor index, first count test percentage and T50, respectively. The hydration for 60 minutes followed by dehydration for 60 minutes gave the best results on seed viability and vigor.

scholarly journals Fruit Description and Evaluation of Five Treatments to Break Seed Dormancy of Brownlowia peltata Benth.

2017 ◽  
Vol 3 (4) ◽  
pp. 46
Author(s):  
Arief Noor Rachmadiyanto ◽  
Peniwidiyanti . ◽  
Prima Wahyu Kusuma Hutabarat
Keyword(s):  
Germination Rate ◽  
Water Immersion ◽  
Block Design ◽  
Seed Viability ◽  
Hot Water ◽  
Average Weight ◽  
Physical Dormancy ◽  
Significant Difference ◽  
Different Temperatures ◽  
And Control

<p class="Els-Abstract-text"><em>Brownlowia peltata </em>Benth. is a native tree from Borneo, in its germination problem was physical dormancy due to the hard pericarp of the fruit. The study aimed to evaluate five treatments for breaking the seed physical dormancy and the fruit description. The experiment was using Randomized Complete Block Design (RCBD) with four different temperatures of water immersion, a scarification, and control. The fruit of <em>B. peltata</em> was a woody follicle; belong to yellow green group 148 A in color; 29.64 mm in long; 21.62 mm in thickness; 29.86 mm in wide, with an average weight of 12.05 g. Hot water immersion treatment of 40 °C for 60 s; 60 °C for 60 s and 80 °C for 30 s could raise the fruit moisture content of 0.92 % to 1.04 %. There was a significant difference on germination rate coefficient and coefficient of uniformity of germination among treatments. The mature seed does not have a physical dormancy once the carpel splits and create a slit (fruit opening).</p><div><p class="Els-keywords"><strong>Keywords:</strong> <em>Brownlowia pellata </em>Benth; dormancy; fruit; seed; viability.</p></div>

scholarly journals Application of Young Coconut Water and “Kascing” Fertilizer to Red Palm Seed Germination

2017 ◽  
Vol 18 (1) ◽  
pp. 17
Author(s):  
Fatimatuz Zuhro ◽  
Hasni Ummul Hasanah ◽  
Sukadi Sukadi
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Water Concentration ◽  
Germination Percentage ◽  
Coconut Water ◽  
Randomized Design ◽  
Completely Randomized Design

Redpalm isornamentplantwhichpotentiallytobecultured.Thescarcityofredpalmmadeithashighmarket value and need to be preserved. However, the germination growth of red palm is slower than other kinds of palm, so the best way to germinate the red palm seed is needed. The aim of this research is to determine the effect of apliccation some young coconut water concentration and kascing fertilizer to red palm seed germination. The research used factorial completely randomized design with 5 replication, the first factor is young coconut water concentration (0%, 25%, 50%, 75%, and 100%) and the second factor is germination media (steril sand and kascing fertilizer). Variables measured were live germination percentage, speed germination rate, radicula length, and plumula height. Results of this study showed that the best germination media is steril sand and 0% concentration of young coconut water.

scholarly journals Morphometry and Germination of Passovia pyrifolia, Struthanthus marginatus and Phoradendron mucronatum Diaspores

2018 ◽  
Vol 36 (0) ◽  
Author(s):  
J.M.L. AZEVEDO ◽  
C.G.G. SANTOS ◽  
C.S. CAIRES ◽  
J.C. ARAÚJO NETO ◽  
R.C. SOUZA
Keyword(s):  
Moisture Content ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Essential Characteristic ◽  
Germination Time ◽  
Randomized Design ◽  
Different Temperatures ◽  
Mean Germination Time ◽  
Completely Randomized Design

ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.

scholarly journals PENGARUH PERLAKUAN SKARIFIKASI TERHADAP DAYA KECAMBAH TANAMAN SAGA POHON (Adenanthera pavonina L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 27-34
Author(s):  
Cindy Yurike Aprilia, Liman, Muhtarudin dan Agung Kusuma Wijaya
Keyword(s):  
Analysis Of Variance ◽  
Germination Rate ◽  
Hot Water ◽  
Distilled Water ◽  
Hard Seed ◽  
Adenanthera Pavonina ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test

This research aimed to investigate the effect of various scarification treatments on the germination of tree saga seeds (Adenanthera pavonina L.). This study used a completely randomized design (CRD) with 6 treatments and 3 replications. The treatments in this study were P0 (control), P1 (soaked using distilled water for 24 hours), P2 (soaked using hot water 60 °C for 15 minutes), P3 (sanded skin), P4 (soaked using 1% H2SO4 for 30 minutes), and P5 (soaked using goat urine for 30 minutes).  The data obtained were analyzed by Analysis of Variance and continued with the Least Significant Difference Test (LSD) level of 5%. The results showed that the treatment had a very significant effect (P <0.01) on germination rate. The best treatment that can increase the germination of a tree saga seeds (Adenanthera pavonina L.) was by eroding the seed skin using sandpaper. This treatment can produce an average germination of 78.89%, while the treatment that produce the highest abnormal seeds was found in the treatment by soaking using 1% H2SO4 for 30 minutes.  The treatment that produce normal seeds, dead seeds, and the highest fungus-stricken seeds were found in the treatment by eroding the seed skin using sandpaper. The treatment that produced the highest hard seed was control.   Keywords: Germination, Saga, Scarification.

scholarly journals Germination and Viability of Seeds of Caesalpinia pulcherrima Newly Harvested and Stored

2019 ◽  
Vol 11 (7) ◽  
pp. 73
Author(s):  
Janaina S. Ferro ◽  
Dougllas F. Rocha ◽  
João Luciano A. M. Junior ◽  
João C. A. Neto ◽  
José V. Silva ◽  
...  
Keyword(s):  
Germination Percentage ◽  
Water Volume ◽  
Tetrazolium Salt ◽  
Shrub Species ◽  
Viability Analysis ◽  
Randomized Design ◽  
Significant Difference ◽  
Medicinal Properties ◽  
Completely Randomized Design ◽  
Temperature Storage

Caesalpinia pulcherrima is an exotic shrub species, belonging to the Fabaceae family, that has medicinal properties, and is widely used for urban afforestation. The objective of this research was to evaluate the overcoming of the C. pulcherrima seed dormancy, the influence of temperature, storage, and water quantity on the substrate in the germination of the species, as well as the use of the tetrazolium test for viability analysis. The analyzes were carried out at the Plant Propagation Laboratory at the Agricultural Sciences Center of the Federal University of Alagoas, in the municipality of Rio Largo, Brazil, and at the Laboratory of Plant Physiology, Arapiraca Campus, at the same University. The experiments were performed under a completely randomized design, with four replicates of 25 seeds. The results demonstrated that during storage the seeds developed a possible secondary dormancy, which was overcome with a time of twelve months of storage. The C. pulcherrima seeds subjected to the test of light qualities presented a significant difference in their germination percentage when verified with the time of storage. In the far-red quality, the newly harvested seeds had a germination percentage of (98%), higher than seeds with 12 months of storage (80.5%). The water volume 3.5-fold the weight of the dry paper provides (90%) germination when compared to other volumes. The tetrazolium salt concentrations of 0.075% and 0.1%, under the temperature of 30&deg;C within 2 hours, are indicated for the viability analysis of Caesalpinia pulcherrima seeds.

scholarly journals Effects of Seed Size on the Germination and Early Growth of African Baobab (Adansonia digitata L.)

2020 ◽  
pp. 1-7
Author(s):  
J. I. Amonum ◽  
F. Sumaduniya ◽  
T. O. Amusa
Keyword(s):  
Leaf Area ◽  
Seed Size ◽  
Germination Rate ◽  
Germination Percentage ◽  
Early Growth ◽  
Adansonia Digitata ◽  
Size Classes ◽  
Randomized Design ◽  
Significant Difference ◽  
Viable Seeds

The effects of seed size on the germination and early growth of A. digitata were assessed. Seeds were sourced locally within the University of Ilorin Campus. One hundred viable seeds were selected from each of the size classes. The seed weight, length, breadth, and width were measured for all the selected seeds. Mean weight, length, breadth, and thickness were: 0.62 g, 9.16 mm, 7.45 mm, and 5.85 mm, respectively for small seeds. 0.41 g, 9.54 mm, 7.68 mm, and 6.03 mm, respectively for medium seeds; and 0.44 g, 9.92 mm, 7.89 mm, and 6.21 mm, respectively for large seeds. The experiment was laid out in a completely randomized design (CRD). Seed dormancy was broken through the acid scarification method by soaking in H2SO4 at 70% concentration for 20 minutes with constant stirring. Variables of interest include: leaves number, seedlings height, collar diameter and leaf area. Based on the results of this finding, medium and large seeds germinated in 7 days after sowing while small seeds germinated in 10 days after sowing. Large seeds had the highest germination percentage (55%), followed by medium seeds with 27%. The small seeds were the least in terms of germination rate (21%). There was no significant difference in mean seedling heights, collar diameters and number of leaves among the seed size classes (P˃0.05). Mean leaf area significantly differed among the three size classes (P˂0.05). The result showed that large seeds gave best germination at a shorter period than the medium and small seed sizes. Thus, large seeds of A. digitata are recommended in propagation activities involving the species.

scholarly journals EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

2021 ◽  
pp. 1-10
Author(s):  
A Abdulrahman ◽  
M Atiku ◽  
A.S Ambursa ◽  
I Umar ◽  
M Muhammed ◽  
...  
Keyword(s):  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hot Water ◽  
State University ◽  
Detarium Microcarpum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

scholarly journals Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

2017 ◽  
Vol 23 (1) ◽  
pp. 15 ◽  
Author(s):  
Vanessa Stegani ◽  
Guilherme Augusto Cito Alves ◽  
Douglas Junior Bertoncelli ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Liquid Nitrogen ◽  
Dry Mass ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Blotting Paper ◽  
Cryogenic Solutions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Cryoprotective Solution

The objective was to evaluate the use of cryogenic solutions in cryopreservation Sinningia leucotricha seeds by the vitrification method in liquid nitrogen. The treatments were: T1 - control: without cryoprotective solution; T2 - PVS1; T3 - modified PVS1; T4 - PVS2; T5 - modified PVS2; T6 - PVS3; T7 - PVS3 modified; T8 - PVS2 + 1% phloroglucinol. After 15 days of immersion of seeds in LN, the cryotubes were removed and rapidly reheated to a temperature of 40 °C water bath for 1.5 minutes. Then, the seeds were washed with wash solution for 20 min. Later they were submitted to the germination test which was conducted on blotting paper moistened with distilled water packaged in crystal polystyrene boxes kept in a growth chamber at 25 ± 2 °C and 16 hours photoperiod. Was evaluated the germination percentage the germination speed index (GSI), and at the end of the experiment will determine the length of shoot (LS) and root (LR), and dry mass of seedlings (DMS). We used a completely randomized design with eight treatments and five replications, consisting of 100 seeds. The direct submission of the queen of the abyss seeds in liquid nitrogen provided the highest germination values, GSI, LS, LR and DMS. The queen of the abyss seeds can be cryopreserved in liquid nitrogen directly without the need to cryoprotectant solutions.

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