scholarly journals Effects of aqueous plant extracts and inorganic fertilizer on the germination, growth and development of maize (Zea mays L.)

2014 ◽  
Vol 59 (3) ◽  
pp. 243-254 ◽  
Author(s):  
Kanayo Chukwuka ◽  
Maxwell Obiakara ◽  
Israel Ogunsumi
Keyword(s):  
Plant Extracts ◽  
Growth And Development ◽  
Tap Water ◽  
Germination Percentage ◽  
Distilled Water ◽  
Aqueous Extracts ◽  
Radicle Growth ◽  
Randomized Design ◽  
Npk Fertilizer ◽  
Completely Randomized Design

Screen house experiments were carried out to examine the effects of aqueous leaf extracts of Tithonia diversifolia (Ti) and Vernonia amygdalina (Ve), as well as NPK fertilizer (15-15-15) on the germination, growth and development of maize. Fresh leaves were collected, washed with tap water, chopped and pounded, soaked in distilled water and filtered. The two filtrates were used to prepare extracts at 50 and 100% w/v. Four maize seeds were placed in Petri dishes laid out in a completely randomized design with five replicates. In every Petri dish 10 ml of extract per treatment was added. A control experiment with distilled water was also set up. Also, in a completely randomized design with four replicates, 4-week-old potted maize plants were treated with 500 ml of each extract as well as with 1.52 g of NPK fertilizer. The results showed that the germination percentage of the seeds followed the order Ti50 - Control - Ve50. The seeds treated with aqueous extracts of T. diversifolia and V. amygdalina at 100% w/v produced lower but equal germination percentage. The seedling radicle growth was significantly inhibited by the aqueous extracts of Ti100, Ve50 and Ve100 (p?0.05). The inhibition was dose-dependent and more pronounced in seeds treated with extracts of V. amygdalina. The aqueous extract of T. diversifolia (50% w/v) and control influenced radicle growth substantially. All the extracts inhibited the plumule development compared to the control. On the other hand, growth, development and yield were not significantly affected by the plant extracts.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

scholarly journals EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

2021 ◽  
pp. 1-10
Author(s):  
A Abdulrahman ◽  
M Atiku ◽  
A.S Ambursa ◽  
I Umar ◽  
M Muhammed ◽  
...  
Keyword(s):  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hot Water ◽  
State University ◽  
Detarium Microcarpum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

scholarly journals Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

2017 ◽  
Vol 23 (1) ◽  
pp. 15 ◽  
Author(s):  
Vanessa Stegani ◽  
Guilherme Augusto Cito Alves ◽  
Douglas Junior Bertoncelli ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Liquid Nitrogen ◽  
Dry Mass ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Blotting Paper ◽  
Cryogenic Solutions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Cryoprotective Solution

The objective was to evaluate the use of cryogenic solutions in cryopreservation Sinningia leucotricha seeds by the vitrification method in liquid nitrogen. The treatments were: T1 - control: without cryoprotective solution; T2 - PVS1; T3 - modified PVS1; T4 - PVS2; T5 - modified PVS2; T6 - PVS3; T7 - PVS3 modified; T8 - PVS2 + 1% phloroglucinol. After 15 days of immersion of seeds in LN, the cryotubes were removed and rapidly reheated to a temperature of 40 °C water bath for 1.5 minutes. Then, the seeds were washed with wash solution for 20 min. Later they were submitted to the germination test which was conducted on blotting paper moistened with distilled water packaged in crystal polystyrene boxes kept in a growth chamber at 25 ± 2 °C and 16 hours photoperiod. Was evaluated the germination percentage the germination speed index (GSI), and at the end of the experiment will determine the length of shoot (LS) and root (LR), and dry mass of seedlings (DMS). We used a completely randomized design with eight treatments and five replications, consisting of 100 seeds. The direct submission of the queen of the abyss seeds in liquid nitrogen provided the highest germination values, GSI, LS, LR and DMS. The queen of the abyss seeds can be cryopreserved in liquid nitrogen directly without the need to cryoprotectant solutions.

scholarly journals Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dry Matter ◽  
Folk Medicine ◽  
Germination Percentage ◽  
Climate Variations ◽  
Randomized Design ◽  
Mother Plants ◽  
Physiological Quality ◽  
Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

scholarly journals Uji efektivitas kombinasi pupuk bio-slurry dengan pupuk npk terhadap pertumbuhan dan produksi dua varietas padi sawah (oryza sativa L.)

2018 ◽  
Vol 2 (2) ◽  
pp. 188
Author(s):  
Ahmad Khanafi ◽  
Yafizham Yafizham ◽  
Didik Wisnu Widjajanto
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Growth And Yield ◽  
Production Data ◽  
Single Treatment ◽  
Randomized Design ◽  
Npk Fertilizer ◽  
Completely Randomized Design ◽  
Fertilizer Combination ◽  
Range Test

The objective of this research was to investigate the effectiveness of combination of bio-slurry fertilizer and NPK fertilizer on the growth and production of two varieties of rice. The experiment was assigned in a completely randomized design of factorial pattern. The first factor was the combination of bio-slurry and NPK fertilizer that consisted of P0 = no added fertilizer (control) 0 ton/ha, P1 = NPK fertilizer 550 kg/ha (165 kg N, 33 kg P, 45 kg K); P2 = bio-slurryfertilizer 2.3 tons/ha (45 kg N, 14 kg P, 23 kg K) and NPK fertilizer 400 kg/ha (120 kg N, 24 kg P, 32 kg K); P3 = bio-slurryfertilizer 4.6 tons/ha (90 kg N, 28 kg P, 46 kg K) and NPK fertilizer 250 kg/ha (75 kg N, 15 kg P, 20 kg K); P4 = bio-slurry fertilizer 5.9 tons/ha (115 kg N, 36 kg P, 59 kg K) and NPK fertilizer 100 kg/ha (30 kg N, 6 kg P, 8 kg K); and P5 = bio-slurryfertilizer 8.5 tons/ha (165 kg N, 52 kg P, 85 kg K). The second factor was varieties of rice that consisted of V1 : IR-64 and V2 : Ciherang. Each treatment was repeated three times. Parameters observed were plant height, number of tillers, weight of 1.000 grains, and rice production. Data were analyzed using ANOVA and continued with Duncan’s Multiple Range Test (DMRT). The results showed that the combination of bio-slurry fertilizer and NPK fertilizer were significantlyaffect (p <0.05)all observation parameters, while varieties of wetland rice did not show significant effect on all observation parameters (p<0.05). The application of bio-slurry fertilizer in single treatment or in either combination with NPK fertilizer had the same result with the treatment of NPK fertilizer on the growth and yield of rice. Keywords: rice, fertilizer combination, bio-slurry fertilizer, NPK fertilizer

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

2021 ◽  
Vol 5 (1) ◽  
pp. 11-17
Author(s):  
Febiasasti Trias Nugraheni ◽  
Erma Prihastanti ◽  
Endah Dwi Hastuti
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Storage Temperature ◽  
Germination Percentage ◽  
Plasma Radiation ◽  
Agricultural Commodity ◽  
Time Duration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Storage

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

scholarly journals Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of Deteriorated Soybean Seed

2018 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
P.K. Dewi Hayati
Keyword(s):  
Seed Viability ◽  
Soybean Seed ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Time Data ◽  
Randomized Design ◽  
Vigor Index ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Hydration And Dehydration

Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integrated with rhizobateri to viability and vigor of deteriorated soybean seed.  A completely randomized design with four replicates were used in this experiment. Seeds were treated with varied combination of hydration period in a rhizobacteria suspension and followed by dehydration time.  Data were analysed using the F-test and significant differences were further tested with Least Significant Difference at the 5% level. Results showed that the hydration and dehydration period of seeds in a rhizobacteria suspension improved viability and vigor of seed which have 59.5% initial germination percentage. The improvement was 12.28%, 0.56, 25.4% and 1.4 days for standard germination test, vigor index, first count test percentage and T50, respectively. The hydration for 60 minutes followed by dehydration for 60 minutes gave the best results on seed viability and vigor.

scholarly journals Plastochron and yield of Physalis peruviana L. grown in different environments and transplanting dates

2020 ◽  
Vol 41 (4) ◽  
pp. 1151
Author(s):  
André Ricardo Zeist ◽  
Juliano Tadeu Vilela de Resende ◽  
Daniel Suek Zanin ◽  
Israel Felipe Lustosa da Silva ◽  
Daniel Fernandes da Silva ◽  
...  
Keyword(s):  
Leaf Area ◽  
Growth And Development ◽  
Temperature Data ◽  
Node Number ◽  
Physalis Peruviana ◽  
Yield And Quality ◽  
Randomized Design ◽  
Crop Cycle ◽  
Completely Randomized Design ◽  
Reproductive Phases

This study aimed to estimate plastochron, leaf area, and yield of Physalis peruviana seedlings grown in different environments and transplanted on different dates. The experiment was carried out in a completely randomized design, arranged in a 2 x 3 factorial scheme, namely: two cultivation environments (protected and field) and three transplanting dates (10/27/2013, 12/15/2013, and 02/11/2014). Four replicates of eight plants were used per plot, and the four central plants composed the useful area. Based on mathematical models and temperature data, plastochron was estimated for the entire crop cycle and vegetative and reproductive phases separately. Leaf area and node number were measured, and their relationship established at the end of the study. Other variables assessed were fruit number, mass, and yield per plant. Our results showed that the higher temperatures in the protected environment anticipated phenological timings. Plastochron values of 60.0 and 16.5 °C day-1 can be used during vegetative and reproductive stages, respectively. The protected environment increased vegetative growth and development, as well as yield of P. peruviana seedlings. In the Guarapuava-PR region, P. peruviana seedlings should be transplanted during the spring so that higher fruit yield and quality could be achieved.

Effects of crude aqueous extracts of indigenous pesticidal plants on the ladybird beetle, Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae)

2018 ◽  
Vol 38 (02) ◽  
pp. 159-167
Author(s):  
E. Mazhawidza ◽  
B. M. Mvumi ◽  
U. Mazarura
Keyword(s):  
Plant Extracts ◽  
Tap Water ◽  
Datura Stramonium ◽  
Negative Control ◽  
Aqueous Extracts ◽  
Ladybird Beetle ◽  
Post Exposure ◽  
Application Rates ◽  
Hippodamia Variegata ◽  
Laboratory Bioassays

AbstractEffects of pesticidal plant extracts on non-targeted species are rare. Direct topical and residual sprays of crude aqueous extracts of three pesticidal plants: Datura stramonium L. Bobgunnia madagascariensis Kirkbr. &amp; Wiersema and Solanum delagoense L. were assessed in laboratory bioassays and on-station experiments against the ladybird beetle, Hippodamia variegata (Goeze). The plants are routinely used by smallholder vegetable farmers to control aphids, Brevicoryne brassicae L. attacking rape, Brassica napus L. The crude extracts of D. stramonium fresh leaves, S. delagoense fresh fruits and B. madagascariensis dried pods, were applied separately at 5, 10, 15, 20 and 25% w/v under laboratory conditions. Application rates of 20% and 25% w/v of the plant extracts were further evaluated on-station. Negative (tap water) and positive (dimethoate® (36% E.C)) controls were included for comparison. The mortality of H. variegata was recorded 24, 48 and 72 h post-exposure. In on-station experiments, treatments were applied fortnightly and live H. variegata adults were counted at 1, 7 and 14 days post-application. Mortality of H. variegata in laboratory bioassays increased with increase in post-exposure time and B. madagascariensis (25% w/v) caused the highest mortality. Based on LD50 values, B. madagascariensis extracts were most toxic (LD50, 30% w/v) followed by D. stramonium (LD50, 34% w/v) and S. delagoense (LD50, 49% w/v) 24 h post-application. In on-station experiments, the synthetic chemical significantly lowered (P &lt; 0.05) H. variegata numbers compared to the negative control and plant extracts. The results showed that D. stramonium and S. delagoense extracts at the application rates used in the study were relatively safer to H. variegata than B. madagascariensis (25% w/v); hence, the former two plants can be included in integrated pest management programmes.

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