scholarly journals CARPOSEMINAL BIOMETRY AND GERMINATION OF Cabralea canjerana (Vell.) Mart.

FLORESTA ◽  
2017 ◽  
Vol 47 (4) ◽  
pp. 391 ◽  
Author(s):  
Helena Cristina Rickli-Horst ◽  
Manoela Mendes Duarte ◽  
Cacilda João Chirinzane ◽  
Antonio Carlos Nogueira
Keyword(s):  
Native Species ◽  
Germination Rate ◽  
Potential Temperature ◽  
Dry Mass ◽  
Germination Percentage ◽  
Germination Test ◽  
Mean Values ◽  
Blotting Paper ◽  
Mean Germination Time ◽  
Digital Caliper

Cabralea canjerana is a species that has ecological and economic importance for the Southern Brazil. This study aimed to characterize the biometry of fruits and seeds as well as the C. canjerana germinative process submitted to different substrates and temperatures. The biometric characteristics of fruits and seeds were analyzed with the aid of digital caliper. In the germination test, the substrates blotting paper, sand and vermiculite, and the temperatures of 20, 25 and 30 ºC were used. The germination percentage, mean germination rate (MGR), mean germination time (MGT), synchronization index (SI), fresh mass (FS) and dry mass (DM) of seedlings were assessed. The highest germination percentages occurred for vermiculite at three temperatures, blotting paper at 25 °C and sand at 20 and 30 °C. There was seedling formation in all treatments. The use of the sand substrate and the temperatures of 25 and 30 °C provided lower mean values of MGT and SI and higher mean values of MGR. The highest DM occurred with sand at the temperature of 20 ºC. The biometric characteristics of fruits and seeds varied. We recommend the use of vermiculite at temperatures of 20, 25 and 30 ºC, blotting paper at 25 °C and sand at 20 and 30 °C for germination tests.Keywords: Meliaceae, native species, germination potential, temperature, substrate. Resumo:Biometria carpo seminal e germinação de Cabralea canjerana (Vell.) Mart. Cabralea canjerana é uma espécie que possui importância ecológica e econômica para o Sul do Brasil. Este estudo objetivou caracterizar a biometria de frutos e sementes, assim como o processo germinativo de C. canjerana submetido a diferentes substratos e temperaturas. Analisaram-se as características biométricas de frutos e sementes com auxílio de paquímetro digital. No teste de germinação, foram utilizados os substratos papel mata-borrão, areia e vermiculita e as temperaturas de 20, 25 e 30 ºC, avaliando-se a porcentagem de germinação, velocidade média de germinação (VMG), tempo médio de germinação (TMG), índice de sincronização (I), massa fresca (MF) e massa seca (MS) de plântulas. As maiores porcentagens de germinação ocorreram com vermiculita nas três temperaturas, papel mata-borrão a 25 °C e areia a 20 e 30 °C. Houve formação de plântulas em todos os tratamentos. O uso do substrato areia e das temperaturas de 25 e 30 ºC proporcionou menores médias de TMG e I e maiores médias de VMG. A maior MS ocorreu utilizando-se areia sob temperatura de 20 ºC. Ocorreu variação das características biométricas de frutos e sementes. Recomenda-se a utilização de vermiculita nas temperaturas de 20, 25 e 30 ºC, papel mata-borrão a 25 °C e areia a 20 e 30 °C para testes de germinação.Palavras-chave: Meliaceae, espécie nativa, potencial germinativo, temperatura, substrato.

scholarly journals Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

2017 ◽  
Vol 23 (1) ◽  
pp. 15 ◽  
Author(s):  
Vanessa Stegani ◽  
Guilherme Augusto Cito Alves ◽  
Douglas Junior Bertoncelli ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Liquid Nitrogen ◽  
Dry Mass ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Blotting Paper ◽  
Cryogenic Solutions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Cryoprotective Solution

The objective was to evaluate the use of cryogenic solutions in cryopreservation Sinningia leucotricha seeds by the vitrification method in liquid nitrogen. The treatments were: T1 - control: without cryoprotective solution; T2 - PVS1; T3 - modified PVS1; T4 - PVS2; T5 - modified PVS2; T6 - PVS3; T7 - PVS3 modified; T8 - PVS2 + 1% phloroglucinol. After 15 days of immersion of seeds in LN, the cryotubes were removed and rapidly reheated to a temperature of 40 °C water bath for 1.5 minutes. Then, the seeds were washed with wash solution for 20 min. Later they were submitted to the germination test which was conducted on blotting paper moistened with distilled water packaged in crystal polystyrene boxes kept in a growth chamber at 25 ± 2 °C and 16 hours photoperiod. Was evaluated the germination percentage the germination speed index (GSI), and at the end of the experiment will determine the length of shoot (LS) and root (LR), and dry mass of seedlings (DMS). We used a completely randomized design with eight treatments and five replications, consisting of 100 seeds. The direct submission of the queen of the abyss seeds in liquid nitrogen provided the highest germination values, GSI, LS, LR and DMS. The queen of the abyss seeds can be cryopreserved in liquid nitrogen directly without the need to cryoprotectant solutions.

scholarly journals Phytotoxicity of Chitosan and SiO2 Nanoparticles to Seed Germination of Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) Plants

2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH
Keyword(s):  
Germination Rate ◽  
Dry Weight ◽  
Sio2 Nanoparticles ◽  
Triticum Aestivum L ◽  
Germination Percentage ◽  
Hordeum Vulgare L ◽  
Randomized Design ◽  
Direct Exposure ◽  
Contrast Exposure ◽  
Mean Germination Time

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  

scholarly journals Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Gabriel Streck Bortolin ◽  
Alfredo José Salinas-Arcienega ◽  
Yutcelia Carolina Galviz-Fajardo ◽  
Luciano do Amarante ◽  
Carlos Eduardo da Silva Pedroso ◽  
...  
Keyword(s):  
Seed Germination ◽  
Physiological Responses ◽  
Dry Mass ◽  
Germination Percentage ◽  
Early Growth ◽  
Paspalum Notatum ◽  
Water Restriction ◽  
Germination Test ◽  
Growth Activity ◽  
Higher Temperature

ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).

scholarly journals Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  
Keyword(s):  
Dry Mass ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Randomized Design ◽  
Temperature Regimes ◽  
Seedling Length ◽  
Physiological Quality ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

scholarly journals Morphological and germination response variability in seeds of wild yellow gentian (Gentiana lutea L.) accessions from northwest Spain

Botany ◽  
2012 ◽  
Vol 90 (8) ◽  
pp. 731-742 ◽  
Author(s):  
Félix Pérez-García ◽  
Federico Varela ◽  
M. Elena González-Benito
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Germination Rate ◽  
Seed Mass ◽  
Germination Percentage ◽  
Gentiana Lutea ◽  
Moist Chilling ◽  
Mean Germination Time ◽  
Wild Accessions ◽  
Seed Water Content

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.

scholarly journals Plant growth regulators in the germination of Diospyros hispida A. DC. (EBENACEAE) seeds

2021 ◽  
Vol 10 (2) ◽  
pp. e27110212471
Author(s):  
Aline Bueno Ramalho ◽  
Lúcia Filgueiras Braga
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Rate ◽  
Dry Mass ◽  
Shoot Length ◽  
Distilled Water ◽  
Seedling Production ◽  
Mean Germination Time

Plant growth regulators in the germination of Diospyros hispida A. DC. (Ebenaceae) Seed. Diospyros hispida species is a native fruit that has long germination period, hindering seedling production. The aim of this work was to evaluate the germination of Diospyros hispida seeds under the effect of gibberellic acid and biostimulant applications. Four replicates of 25 seeds per treatment were used. Treatments were: T1 - distilled water, T2, T3 and T4 - GA3 solutions at 100, 150 and 200 mg L-1, respectively, T5 - Immersion for 24 hours in distilled water with aeration, T6, T7 and T8 - Immersion for 24 hours in aerated GA3 solutions at 100, 150 and 200 mg L-1, respectively, T9, T10 and T11 - biostimulant application (containing auxin, gibberellin and cytokinin) at 3, 5 and 7 ml kg-1 of seeds. Germination rate, mean germination time, germination speed index, synchronization index, relative germination frequency, normal seedlings, root length, shoot length and seedling dry mass were evaluated. The effect of gibberellic acid and biostimulant containing auxin, gibberellin and cytokinin negatively influenced Diospyros hispida germination under the conditions of this study.

scholarly journals Maturation and germination of Vernonanthura discolor seeds

2016 ◽  
Vol 40 (2) ◽  
pp. 164-172
Author(s):  
Camila Ribeiro de Souza Grzybowski ◽  
Rosemeire Carvalho da Silva ◽  
Elisa Serra Negra Vieira ◽  
Maristela Panobianco
Keyword(s):  
Native Species ◽  
Dry Matter ◽  
Reproductive Development ◽  
Dry Weight ◽  
Harvest Time ◽  
Germination Test ◽  
Physiological Maturity ◽  
Light Regimes ◽  
Blotting Paper ◽  
Periodic Monitoring

ABSTRACT Vernonanthura discolor is a native species with potential for reclamation of degraded lands, and the manufacture of wood panels. This study has aimed to evaluate the process of maturation and germination of Vernonanthura discolor seeds, determining the harvest time, and conducting the germination test. A periodic monitoring of the reproductive development of sources of Vernonanthura discolor was conducted, located in the municipality of Bocaiúva do Sul, PR, Brazil; and starting from the anthesis, weekly collections of seeds were performed to evaluate the percentage of dry matter, germinating power, and vigor, in order to identify the physiological maturity of the seeds. In the germination test, two combinations of four temperatures (20, 30, 20-30 and 30 °C), and two light regimes were tested, seeking to define the best methodology and evaluation dates of the test. It was concluded that the seeds physiological maturity Vernonanthura discolor reached 45 days after the anthesis, when the maximum values of dry weight, germination and vigor are achieved. Germination test may be conducted on blotting paper at 20 or 25 °C with a supply of light, or at 25 °C in the dark, the first count being on the 13th and the last one on the 29th day after seeding.

scholarly journals Effect of GA3, KNO3, and removing of basal point of seeds on germination of sweet granadilla (Passiflora ligularis Juss) and yellow passion fruit (Passiflora edulis f. flavicarpa)

2013 ◽  
Vol 35 (3) ◽  
pp. 853-859 ◽  
Author(s):  
Julián Cárdenas ◽  
Carlos Carranza ◽  
Diego Miranda ◽  
Stanislav Magnitskiy
Keyword(s):  
Leaf Area ◽  
Germination Rate ◽  
Dry Mass ◽  
Passion Fruit ◽  
Initial Growth ◽  
Passiflora Edulis ◽  
Mean Germination Time ◽  
Previously Treated ◽  
The Mean ◽  
Yellow Passion Fruit

Passiflora seeds germinate erratically presenting difficulties for their handling in a greenhouse. The effect of removing of basal point of seeds (RB) and pre-imbibition of seeds of sweet granadilla and yellow passion fruit in 50, 100, 200, and 400 mg mL-1 solutions of gibberellic acid (GA3) or 0.1% KNO3 solution was studied. The experiment was conducted in greenhouses in La Plata, Colombia. Two accessions PrJ1 and PrJ2 of sweet granadilla were evaluated. There were calculated the final percentage of germination (PG), mean germination time (MGT), and the mean germination rate (MGR). The leaf area and dry mass of seedlings were measured 22 days after sowing (das); with this data, specific leaf area and relation root/shoot were calculated. In all cases, the highest germination percentages were achieved treating seeds with KNO3 (89, 92, and 87% for yellow passion fruit, PrJ2, and PrJ1, respectively), but the increase in MGR (3.3 germinated seeds per day) and the decrease in MGT (16 days) were only significant for PrJ1. RB had a significant reduction of PG in all cases (28, 12, and 33% for passion fruit, PrJ2 and PrJ1, respectively). With the increase in the concentration of GA3, PG was reduced for two accessions of sweet granadilla, for yellow passion fruit this trend was not clear, no treatment with GA3 showed significant differences with the control. Leaf area (24.07 cm2) and dry mass of seedlings (135 mg) were significantly higher than seeds previously treated with KNO3 only for PrJ1.The solution of KNO3 0,1% is recommended to improve the germination and initial growth of granadilla seedlings.

scholarly journals Conditioning in the promotion and uniformization of Umbu seed germination

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
Renato Teo Barros ◽  
Cibele Chalita Martins ◽  
Francisco Elder Carlos Bezerra Pereira ◽  
Givanildo Zildo da Silva
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Water Content ◽  
Environmental Conditions ◽  
Seed Quality ◽  
Germination Rate ◽  
Ambient Conditions ◽  
Germination Test ◽  
Mean Germination Time ◽  
Cold Chamber

Abstract Seed dormancy may decrease during storage and some environmental conditions may accelerate this process. The aim of this work was to determine efficient techniques to condition umbu seeds in order to promote and standardize their germination. Seeds were stored for 180 days in paper bags kept in five ambient conditions: laboratory (25 ºC and 55% RH); warm oven (40 °C and 53% RH); hot oven (50 °C and 49% RH); dry chamber (18 °C and 65% RH) and cold chamber (10 °C and 65% RH). Seed quality was evaluated every 60 days by means of the following tests and determinations: water content; germination test (25 °C and 55% RH, weekly evaluated up to 91 days after sowing); first count test (14 days); germination rate index; mean germination time and electrical conductivity. The conditioning of umbu seeds in laboratory, or in warm oven (40 ºC) used efficient techniques to promote and standardize germination; under these conditions, after six months of storage, germination increased from 31% to 84 and 74%, respectively.

scholarly journals The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Tomato Seeds ◽  
Germination Characteristics ◽  
Salinity Levels ◽  
Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

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