Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

Annona nutans (Annonaceae) is a plant species found in Bolivia, Paraguay, Argentina, and the Brazilian Cerrado, specifically in the states of Mato Grosso and Mato Grosso do Sul (Brazil). Its common names are Araticû-Mi and Araticû-Ñu. The research contributions regarding the chemical composition and biological activities of extracts from A. nutans are rare, with only four articles being found in the literature. Therefore, the present study evaluated the anti-inflammatory and antinociceptive activities of the hydromethanolic fraction (FHMeOH) using carrageenan-induced paw edema and hot-plate tests. In addition, the antioxidant activity was evaluated by DPPH radical scavenging, total phenolic, flavonoid and tannin content assays and quantification of the major metabolites by LC-MS were performed. Oral treatment with the FHMeOH (at a dose of 300 mg.kg-1) significantly reduced paw edema 2 h and 4 h after the inflammatory stimulus. The intraperitoneal (i.p.) treatment with the FHMeOH (50 and 100 mg.kg-1) proved to be most effective, and the inhibition of acute inflammation was still visible 6 h after carrageenan injection. At doses of 50 and 100 mg.kg-1 (i.p.), FHMeOH exhibits central antinociceptive effects by increasing the latency of the reaction in the hot-plate model. The FHMeOH showed antioxidant potential, and the metabolites quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apiofuranosyl-(1→2)-galactopyranoside, and chlorogenic acid were identified and quantified by LC-MS. Our results indicate, for the first time, that FHMeOH has anti-inflammatory, antinociceptive and antioxidant potential, and it is a promising source of studies for new herbal medicines

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Antioxidant, Anti-Inflammatory, and Analgesic Activities of Aqueous Extract of Diploknema butyracea (Roxb.) H.J. Lam Bark

The Scientific World JOURNAL ◽

10.1155/2020/6141847 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Sumit Bahadur Baruwal Chhetri ◽

Deepa Khatri ◽

Kalpana Parajuli

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging ◽

Total Phenolic ◽

Paw Edema ◽

Hot Plate ◽

Anti Inflammatory ◽

Analgesic Activities ◽

Radical Scavenging Assay

Diploknema butyracea (Roxb.) H.J. Lam is a multipurpose tree used by the Nepalese indigenous people for medicinal purposes such as rheumatism, asthma, and ulcer and other purposes such as cooking and lighting. However, there is no scientific evidence for the medicinal uses of this plant. The present study aimed to explore the phytochemical constituents, estimate the total phenolic content, evaluate antioxidant activity, and investigate the in vivo anti-inflammatory and analgesic activities of aqueous extract of Diploknema butyracea (Roxb.) H.J. Lam bark (ADBB). Phytochemical screening was performed using standard methods. The total phenolic content was determined using the Folin–Ciocalteu method. The in vitro antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay and nitric oxide radical scavenging assay. For the in vivo studies, the plant extract was given in three different doses (50, 100, and 200 mg/kg body weight) to male albino Wistar rats. Anti-inflammatory and analgesic studies were carried out using the carrageenan-induced rat paw edema and the hot plate method, respectively. Results revealed the presence of different phytoconstituents such as flavonoids, tannins, glycosides, terpenoids, and carbohydrates together with a considerable amount of phenolic compounds. Antioxidant assays indicated the potent antioxidant activity of the plant extracts. The higher dose of D. butyracea (200 mg/kg) exhibited a maximum and significant inhibition (53.20%) of rat hind paw edema volume at 4 h and showed a greater increment in latency time (12.15 ± 1.81 sec) in the hot plate test at 120 min. The present study demonstrated the antioxidant, anti-inflammatory, and analgesic potential of ADBB, which supports its traditional medicinal use.

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Potentially Bioactive Metabolites from Pineapple Waste Extracts and Their Antioxidant and α-Glucosidase Inhibitory Activities by 1H NMR

Foods ◽

10.3390/foods9020173 ◽

2020 ◽

Vol 9 (2) ◽

pp. 173 ◽

Cited By ~ 1

Author(s):

Awanis Azizan ◽

Ai Xin Lee ◽

Nur Ashikin Abdul Hamid ◽

Maulidiani Maulidiani ◽

Ahmed Mediani ◽

...

Keyword(s):

1H Nmr ◽

Inhibitory Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ananas Comosus ◽

Bioactive Metabolites ◽

Total Phenolic ◽

Pineapple Waste ◽

Inhibitory Activities ◽

Promising Source

Pineapple (Ananas comosus) waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications. This study strives to characterize the complete metabolite profiles of a variety of MD2 pineapple waste extracts. Metabolomics strategies were utilized to identify bioactive metabolites of this variety prepared with different solvent ratios. Each pineapple waste extract was first screened for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, and α-glucosidase inhibitory activities. The highest TPC was found in all samples of the peel, crown, and core extracted using a 50% ethanol ratio, even though the results were fairly significant than those obtained for other ethanol ratios. Additionally, crown extracted with a 100% ethanol ratio demonstrated the highest potency in DPPH and NO scavenging activity, with IC50 values of 296.31 and 338.52 µg/mL, respectively. Peel extracted with 100% ethanol exhibited the highest α-glucosidase inhibitory activity with an IC50 value of 92.95 µg/mL. Then, the extracts were analyzed and the data from 1H NMR were processed using multivariate data analysis. A partial least squares and correlogram plot suggested that 3-methylglutaric acid, threonine, valine, and α-linolenic acid were the main contributors to the antioxidant activities, whereas epicatechin was responsible for the α-glucosidase inhibitory activity. Relative quantification further supported that 100% crown extract was among the extracts that possessed the most abundant potential metabolites. The present study demonstrated that the crown and peel parts of MD2 pineapple extracted with 100% ethanol are potentially natural sources of antioxidants and α-glucosidase inhibitors, respectively.

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Evaluation of Antioxidant Activities of Alpinia galanga (L.) Willd

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2700 ◽

2018 ◽

Vol 15 (4) ◽

pp. 899-908

Author(s):

Khoirom Ratipiyari Devi ◽

Paonam Priyobrata Singh ◽

Moirangthem Medhapati Devi ◽

Gurumayum Jitendra Sharma

Keyword(s):

Free Radical ◽

Plant Extracts ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Alpinia Galanga ◽

Radical Scavenging Capacity ◽

Scavenging Capacity ◽

Promising Source

Present research was designed to evaluate the free radical scavenging capacities and antioxidant activities of rhizome extracts of Alpinia galanga prepared in different solvent systems (60% aqueous methanol, 60% aqueous ethanol and distilled water) using different in vitro chemical assays. Antioxidant components such as total phenolic content (TPC), total flavonoid content (TFC) and ascorbic acid contents of the ginger species were screened. Antioxidant assays employed included sulphur free radical reactivity assay, ferric ion reducing power assay, DPPH free radical scavenging capacity assay, hydroxyl radical scavenging assay, nitric oxide scavenging activity assay and hydrogen peroxide scavenging assay. The obtained data reveal that the plant extracts contained significant amount of the observed antioxidant components and also exhibited significant free radical scavenging capacities. Methanol (60%) extract exhibited highest antioxidant activity than other solvents. The polyphenolic constituents of the plant extracts appear to be largely responsible for the radical scavenging capacity. The plant extracts act as promising source of antioxidants, and may be useful for development of nutraceuticals and pharmaceutical drugs.

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Antioxidant activities of aqueous extracts from nine different rose cultivars

International Journal of Food Studies ◽

10.7455/ijfs.v7i2.484 ◽

2018 ◽

Vol 7 (2) ◽

Author(s):

Hamadia Khurshid ◽

Syed Mubashar Sabir ◽

Shahid Iqbal Awan ◽

Syed Rizwan Abbas ◽

Muhammad Irshad

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Liver Homogenate ◽

Antioxidant Potential ◽

Total Phenolic ◽

Aqueous Extracts ◽

Dpph Radical ◽

Rose Petals

Rose petals have been applied as food additives in teas, cakes and flavor extracts. The aim of this research study was to explore and reveal the antioxidant potential of aqueous extracts of rose petals belonging to nine genotypes of rose (wild as well as hybrid). The in vitro antioxidant activities of roses were studied by lipid peroxidation assay, DPPH radical scavenging assay, iron chelation assay, phosphomolybdenum reduction assay and total phenolic and flavonoid contents. The aqueous extract showed inhibition against lipid peroxidation (TBARS), induced by prooxidants (10 µM FeSO4) in mice liver homogenate. The free radical scavenging activities of the extracts were determined by scavenging of the DPPH radical. Extracts also showed metal chelating activities and high antioxidant activity in the phosphomolybdenum assay. The high content of phenolics and flavonoids detected in aqueous extracts may be responsible for the antioxidant activity. Amongst the different rose genotypes, screened, Rosa moschata (musk rose) was found to carry slightly higher antioxidant potential, owing to its higher phytochemical content.

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PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL STUDY OF CAPPARIS CARTILAGINEA DECNEFROM YEMEN

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i6.22905 ◽

2018 ◽

Vol 10 (6) ◽

pp. 38

Author(s):

Bushra Abdulkarim Moharram ◽

Hassan M. Al-mahbashi ◽

Riyadh Saif Ali ◽

Faten Ali Aqlan

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Cytotoxic Activity ◽

Human Lung ◽

Biological Activities ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Paw Edema ◽

Anti Inflammatory

Objective: To investigate phytochemicals and biological activities of Capparis cartilaginea extracts.Methods: The methanolic extracts of leaves, stem and twigs of C. cartilaginea were screened for their phytochemicals. The essential oil of the leaves was hydrodistilled by a Clevenger apparatus and analyzed by gas chromatography-mass spectrometry (GC-MS). The leaves extract of C. cartilaginea was evaluated for its anti-inflammatory effect, using formalin-induced paw edema. The leaves, stem and twig extracts were assessed for their antioxidant activity, using free radical scavenging assay, cytotoxic activity, using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and antibacterial activity, using the microdilution method.Results: All extracts of C. cartilaginea contained alkaloids, carbohydrates, protein, coumarin, phytosterols, bitter principles, phenols and tannins. The essential oil of the leaves was mainly composed of isopropyl isothiocyanate (69.4%), butane,1-isothiocyanate (26.97%) and isobutyl isothiocyanate (3.26%). The leaves extract at doses of 200 and 400 mg/kg, significantly inhibited paw edema at the 3rd h (49.1%, 54.0%, respectively) and this effect was comparable to that of diclofenac (58.87%). The leaves extract showed the highest antioxidant activity with IC50 value of 91.71 µg/ml. The twigs extract exhibited the highest cytotoxic activity against human lung carcinoma (A549) with IC50 of 57.5 µg/ml. The leaves and stem extracts exhibited antibacterial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 5.0 mg/ml. Conclusion: The leaves extract of C. cartilaginea is a potential source of bioactive compounds that could have a role in anti-inflammation. Twigs extract of the C. cartilaginea possesses a potential cytotoxic effect on human lung cell line.

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Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity

Antioxidants ◽

10.3390/antiox10071004 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1004

Author(s):

Monika Hejna ◽

Lauren Kovanda ◽

Luciana Rossi ◽

Yanhong Liu

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Feed Additives ◽

Enterotoxigenic Escherichia Coli ◽

Barrier Integrity ◽

Tnf Α ◽

Anti Inflammatory

The objectives of the study were to test the biological activities of peppermint and spearmint oils via (i) measuring in vitro anti-inflammatory effects with porcine alveolar macrophages (PAMs), (ii) determining the barrier integrity of IPEC-J2 by analyzing transepithelial electrical resistance (TEER), (iii) testing their antioxidant activities, and (iv) investigating the antimicrobial activity against enterotoxigenic Escherichia coli (ETEC) F18+. Briefly, (i) macrophages were seeded at 106 cells/mL and treated (24 h) with mint oils and lipopolysaccharide (LPS). The treatments were 2 (0 or 1 μg/mL of LPS) × 5 (0, 25, 50, 100, 200 µg/mL of mint oils). The supernatants were collected for TNF-α and IL-1β measurement by ELISA; (ii) IPEC-J2 cells were seeded at 5 × 105 cells/mL and treated with mint oils (0, 25, 50, 100, and 200 μg/mL). TEER (Ωcm2) was measured at 0, 24, 48, and 72 h; (iii) the antioxidant activity was assessed (0, 1, 50, 100, 200, 500, and 600 mg/mL) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assays; (iv) overnight-grown ETEC F18+ were quantified (CFU/mL) after supplementing with peppermint and spearmint oils (0, 1.44, 2.87, 5.75, 11.50, and 23.00 mg/mL). All data were analyzed using the MIXED procedure. Both mint oils significantly inhibited (p < 0.05) IL-1β and TNF-α secretion from LPS-stimulated PAMs. Mint oil treatments did not affect TEER in IPEC-J2. Spearmint and peppermint oils exhibited (p < 0.05) strong antioxidant activities in DPPH and reducing power assays. Both mint oils also dose-dependently inhibited (p < 0.05) the growth of ETEC F18+ in vitro. The results of the study indicated that both mint oils are great candidate feed additives due to their in vitro anti-inflammatory, antioxidant, and antimicrobial effects. Further research is needed to evaluate their efficacy in vivo.

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Amauroderma rugosum(Blume & T. Nees) Torrend: Nutritional Composition and Antioxidant and Potential Anti-Inflammatory Properties

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/304713 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Pui-Mun Chan ◽

Gowri Kanagasabapathy ◽

Yee-Shin Tan ◽

Vikineswary Sabaratnam ◽

Umah Rani Kuppusamy

Keyword(s):

Submerged Culture ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Indigenous Communities ◽

Antioxidant Potential ◽

Raw264.7 Cells ◽

Total Phenolic ◽

No Production ◽

Freeze Dried ◽

Anti Inflammatory

Amauroderma rugosumis a wild mushroom that is worn as a necklace by the indigenous communities in Malaysia to prevent fits and incessant crying by babies. The aim of this study was to investigate the nutritive composition and antioxidant potential and anti-inflammatory effects ofA. rugosumextracts on LPS-stimulated RAW264.7 cells. Nutritional analysis of freeze-dried mycelia ofA. rugosum(KUM 61131) from submerged culture indicated a predominant presence of carbohydrates, proteins, dietary fibre, phosphorus, potassium, and sodium. The ethanol crude extract (EE), its hexane (HF), ethyl acetate (EAF), and aqueous (AF) fractions of mycelia ofA. rugosumgrown in submerged culture were evaluated for antioxidant potential and anti-inflammatory effects. EAF exhibited the highest total phenolic content and the strongest antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. HF showed dose-dependent inhibition of NO production in LPS-stimulated RAW264.7 cells and NO radical scavenging activity. Gas chromatographic analysis of HF revealed the presence of ethyl linoleate and ergosterol, compounds with known anti-inflammatory properties. In conclusion, the nutritive compositions and significant antioxidant potential and anti-inflammatory effects of mycelia extracts ofA. rugosumhave the potential to serve as a therapeutic agent or adjuvant in the management of inflammatory disorders.

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Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

Open Chemistry ◽

10.1515/chem-2019-0003 ◽

2019 ◽

Vol 17 (1) ◽

pp. 23-30 ◽

Cited By ~ 1

Author(s):

Mert Ilhan ◽

Fatma Tuğçe Gürağaç Dereli ◽

Ibrahim Tümen ◽

Esra Küpeli Akkol

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Folk Medicine ◽

Radical Scavenging ◽

Total Phenolic ◽

Tail Flick ◽

Scavenging Activity ◽

Tail Flick Test ◽

Etoac Extract ◽

Anti Inflammatory

AbstractBryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

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Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Molecules ◽

10.3390/molecules27010314 ◽

2022 ◽

Vol 27 (1) ◽

pp. 314

Author(s):

Jed Jebali ◽

Hanene Ghazghazi ◽

Chedia Aouadhi ◽

Ines ELBini-Dhouib ◽

Ridha Ben Salem ◽

...

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Total Phenolic ◽

Mentha Pulegium ◽

Leaf Extracts ◽

Traditional Remedies

Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

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Phytochemical Screening and Antioxidant Activity of Salvia hispanica

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i41a32314 ◽

2021 ◽

pp. 167-174

Author(s):

Hanane Dib ◽

Meryem Seladji ◽

Fatima Zohra Bencheikh ◽

Meriem Faradji ◽

Chahid Benammar ◽

...

Keyword(s):

Antioxidant Activities ◽

Condensed Tannin ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Total Phenolic ◽

Salvia Hispanica ◽

Salvia Hispanica L ◽

Free Radical Scavenging Assay ◽

Β Carotene

Background: There is a growing interest worldwide to identify novel functional foods able to exert important biochemical activities with low or without toxic effect. Accordingly, the use of Salvia hispanica L. (chia) a Mexican herb, has tremendously grown as an agriculture crop owing to the nutritional and medicinal values. The objective of this study was to evaluate phytochemical composition of chia seeds and prove their claim for functional properties. Materials and Methods: The determination of total phenolic, flavonoid and condensed tannin contents of chia seeds were carried out by the Folin-Ciocalteu, the aluminum trichloride and the vanilline acid spectrometric methods. Antioxidant activities of chia seeds extracts were assessed using free radical scavenging assay DPPH, Ferric Reducing Antioxidant Potential FRAP and β-Carotene bleaching assay. Results: Chia seeds contain high levels of total phenolic and flavonoid contents 19.06±0.14 mg GAE/ g DW and 12.3±0.04 mg CE/g DW, respectively. The content of condensed tannins was estimated at 8.32±0.01 mg CE/g DW. The flavonoid extract showed a higher antioxidant potential against DPPH, FRAP and the bleaching of β-Carotene (0.27±0.00, 0.06±0.03 and 0.39±0.01 mg/mL, respectively). Conclusion: Following the obtained results, we should explore the use of this seeds in food products due its nutritional and chemical composition, possible human health benefits and role as a functional food.

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