scholarly journals A Comparative Study of Antioxidant Potential and Phytochemical Contents of different Extracts of Wild Nasturtium Officinale W T Aiton Collected from Kumaun Region of Uttarakhand

2021 ◽  
Vol 6 (4) ◽  
pp. 298-304
Author(s):  
Pooja Rawal ◽  
Nitisha Negi ◽  
Archana N Sah ◽  
Anchala Guglani
Keyword(s):  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Nasturtium Officinale ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Ferric Reducing Antioxidant Power ◽  
Phytochemical Contents

Nasturtium officinale W. T Aiton or “watercress” (Brassicaceae) is a nutritionally valuable plant that is the richest source of carotenoids, polyphenols, iron, calcium, iodine, folic acid, essential vitamins and minerals. It is traditionally used for their appetiser, anti-scorbutic, stimulant, diuretic and detoxifying properties. The present study was conducted to investigate the antioxidant potential, phytochemical contents (total phenolic, flavonoids and tannin contents) as well as a preliminary phytochemical screening of different extract of aerial parts of Nasturtium officinale collected from the Kumaun region. The different extracts showed significant antioxidant activity as well as total phenolic, flavonoids and tannin contents. The preliminary phytochemical screening showed the presence of several phytochemical constituents such as carbohydrates, proteins, amino acids, glycosides, alkaloids and others. The hydroalcoholic extract possess significantly (P<0.05) higher antioxidant potential with IC50 value (0.333 mg/ml, 0.509mg/ml)and EC50 value (3.537 mg/ml) by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS), and ferric-reducing antioxidant power (FRAP) methods, respectively, as well as highest total tannin, flavonoid and phenolic contents among all the extracts. These results provide substantial evidence that the plant Nasturtium officinalehas antioxidant potential and valuable sources of phytochemicals.

scholarly journals Phytochemicals and Antioxidant Activity in Oat-Buckwheat Dough and Cookies with Added Spices or Herbs

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2267
Author(s):  
Małgorzata Starowicz ◽  
Saruhan Arpaci ◽  
Joanna Topolska ◽  
Małgorzata Wronkowska
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Weight Basis ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Wide Range ◽  
Confectionery Products ◽  
Ferric Reducing Antioxidant Power ◽  
Phytochemical Contents

The aim of this study was to determine the phytochemicals and antioxidant activity in oat-buckwheat doughs and cookies with the addition of ten selected spices or herbs (2 g/100 g flours weight basis). The used spices and herbs, as was expected, showed a wide range of bioactive molecules, namely phenolic acids and flavonoids, and they are a rich source of components with antioxidant potential. All analysed oat-buckwheat dough showed higher antioxidant activity potential and higher total phenolic content (TPC) compared to cookies. The highest TPC was found in clove, both dough and cookies, with its addition showing the highest ferric reducing antioxidant power. Generally, cookies with the addition of spice/herbs showed higher phytochemical contents and antioxidant activity compared to oat-buckwheat cookies without the condiment. The technology of obtaining confectionery products, like oat-buckwheat cookies, that will favor the protection of bioactive compounds should still be improved.

scholarly journals Phenolic Profiles and Antioxidant Activities of 30 Tea Infusions from Green, Black, Oolong, White, Yellow and Dark Teas

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 215 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Guo-Yi Tang ◽  
Shi-Yu Cao ◽  
Xiao-Yu Xu ◽  
Ren-You Gan ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Tea Infusions ◽  
Phenolic Profiles ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power

Tea is among the most consumed drink worldwide, and its strong antioxidant activity is considered as the main contributor to several health benefits, such as cardiovascular protection and anticancer effect. In this study, the antioxidant activities of 30 tea infusions, which were obtained by the mimic of drinking tea of the public, from green, black, oolong, white, yellow and dark teas, were evaluated using ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays, ranging from 504.80 ± 17.44 to 4647.47 ± 57.87 µmol Fe2+/g dry weight (DW) and 166.29 ± 24.48 to 2532.41 ± 50.18 µmol Trolox/g DW, respectively. Moreover, their total phenolic contents (TPC) were detected by Folin-Ciocalteu assay and were in the range of 24.77 ± 2.02 to 252.65 ± 4.74 mg gallic acid equivalent (GAE)/g DW. Generally, Dianqing Tea, Lushan Yunwu Tea, and Xihu Longjing Tea showed the strongest antioxidant activities among 30 teas. Furthermore, the phenolic compounds in tea infusions were identified and quantified, with catechins most commonly detected, especially in green tea infusions, which were main contributors to their antioxidant activities. Besides tea polyphenols, considerable content of caffeine also presented in 30 tea infusions.

scholarly journals Antioxidant poteintial and activity of aerial parts of eight medicinal plants of Uttarakhand, India

2019 ◽  
Vol 48 (2) ◽  
pp. 265-270
Author(s):  
Shakuli Kashyap ◽  
PB Rao ◽  
P Mishra ◽  
Supriya
Keyword(s):  
Radical Scavenging ◽  
Total Phenolic ◽  
Enzymatic Antioxidants ◽  
Antioxidant Power ◽  
Mean Values ◽  
Aerial Parts ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

The antioxidant potential of aerial parts of eight medicinal plant species were investigated by 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Ferrous ion-chelating (FCA) and ferric reducing antioxidant power assays in five different concentrations and the mean values ranged from 20.39 to 83.01%, 17.21 to 48.62% and 11.70 to 21.65 μg Trolox equivalent/mg extract, respectively. Total phenolic and flavonoid contents ranged from 4.83 to 10.46 μg, gallic acid equivalent/mg extract and 1.12 to 13.11 μg quercitin equivalent/mg extract. Enzymatic antioxidants (Unit/mg fresh weight): superoxide dismutase, catalase and peroxidase values ranged from 14.86 to 49.81, 60.00 to 141.33 and 0.25 to 0.60, respectively. Considering the overall assay M. Pudica can be considered as the potent candidate for displaying antioxidant activity among the experimental plants.

scholarly journals Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Omidreza Firuzi ◽  
Katayoun Javidnia ◽  
Maryam Gholami ◽  
Mohammad Soltani ◽  
Ramin Miri
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

Estimation of total Phenolic and ϑlavonoids content and Studies on antioxidant activity of different extracts of Olax scandens by FRAP assay

2020 ◽  
Vol 11 (3) ◽  
pp. 3367-3371
Author(s):  
Pranaya P ◽  
Akiladevi D
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Ic50 Value

Olax scandens Roxb. (family Olacaceae) available in throughout tropical India. The current study, aerial parts of different concentrates of Olax scandens was evaluated for its in-vitro antioxidant potential by FRAP assay taking ascorbic acid as the standard and estimation of total phenolic content and flavonoids content. The IC50 value was originated that methanolic concentrates of Olax scandens are more efficient in antioxidant activity by FRAP methods compared EA & PE concentrates. The methanolic concentrates of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 207µg/ml & 50µg/ml by Ferric reducing ability Power assay. The methanolic and EA concentrates of Olax scandens showed the total phenolic content (14.426 ± 0.032, 4.128 ± 0.025) respectively, and ৒lavonoids content (11.526 ± 0.054, 3.682 ± 0.042) respectively. Invitro antioxidant studies show methanolic concentrates of Olax scandens have better antioxidant activity as well as a higher content of total phenolic and flavonoids content. These results indicate that aerial parts of methanolic concentrates Olax scandens could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

2019 ◽  
Vol 5 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Vinod Kumar Gautam ◽  
Mannu Datta ◽  
Ashish Baldi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Seasonal Variations ◽  
Summer Season ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Spring Season ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

scholarly journals Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3054 ◽  
Author(s):  
Luisa Quesada-Romero ◽  
Carlos Fernández-Galleguillos ◽  
Jan Bergmann ◽  
María-Eugenia Amorós ◽  
Felipe Jiménez-Aspee ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Macrosiphum Euphorbiae ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Ferric Reducing Antioxidant Power ◽  
First Time ◽  
Derivatives Of

Persicaria maculosa (Polygonaceae) (known as lady’s thumb) is an annual morphologically variable weed that is widely distributed in Chile. The purpose of this study was to investigate the antifeedant potential of methanolic (MeOH), ethanolic (EtOH), and dichloromethane (DCM) extracts from the aerial parts of this plant collected in the Valparaíso and Curicó provinces (Chile) and relate this activity to the antioxidant capacity and the presence of phenolic compounds in the extracts. A phenolic profile based on HPLC-ESI-MS/MS allowed the identification of 26 phenolic compounds, most of them glycosyl derivatives of isorhamnetin, quercetin, and kaempferol. In addition, the total phenolic content (TP), total flavonoids (TF), and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion scavenging (O2−), ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) of the extracts are reported. The antifeedant potentials of the plant extracts were tested against Epilachna paenulata, Pseudaletia adultera, Macrosiphum euphorbiae, and Diaphorina citri insects for the first time. The activity against the aphid M. euphorbiae was significant for the DCM extracts of plants from Valparaíso and Curicó (settling % = 23% ± 4% and 23% ± 5%, respectively). The antifeedant activities against the beetle E. paenulata and the lepidoptera P. adultera were significant for Valparaíso extracts, especially when tested against E. Paenulata (IFP = 1.0 ± 0.0). Finally, the MeOH and EtOH extracts from Valparaíso plants reduced the diet consumption of the psilid D. citri (p < 0.05). The results showed that P. maculosa is a good source of flavonoids with some antioxidant capacities and has potential interest as botanical eco-friendly alternative with deterrent activity.

scholarly journals ANTIOXIDANT ACTIVITIES FROM VARIOUS EXTRACTS OF DIFFERENT PARTS OF KELAKAI (STENOCHLAENA PALUSTRIS) GROWN IN CENTRAL KALIMANTAN - INDONESIA

2016 ◽  
pp. 215 ◽  
Author(s):  
Irda Fidrianny ◽  
Siti Kusmardiyani ◽  
Grace Novita
Keyword(s):  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Root Extract ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Young Leaves ◽  
Ferric Reducing Antioxidant Power ◽  
Different Parts

<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant activity from various extracts of different parts of kelakai (Stenochlaena palustris<br />[Burm.f.] Bedd) using two antioxidant testing methods, which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power<br />(FRAP), and correlation of total phenolic contents (TPC), total flavonoid contents (TFC), and total carotenoid contents (TCC) with their inhibitory<br />concentration 50% (IC<br />50<br />) of DPPH and exhibitory concentration 50% (EC<br />50<br />) of FRAP.<br />Methods: Sample was extracted by reflux using different polarity solvents. The extracts were evaporated using vacuum rotary evaporator. Antioxidant<br />activities were tested using DPPH and FRAP assays, determination of TPC, TFC, and TCC was carried out by ultraviolet-visible spectrophotometry, and<br />correlation with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP capacities was analyzed by Pearson’s method.<br />Results: Ethanolic root extract of kelakai (S. palustris) had the lowest IC<br />50<br /> of DPPH scavenging activity 0.8 µg/ml and the lowest EC<br /> of FRAP capacity<br />5.4 µg/ml. Ethanolic kelakai root extract demonstrated the highest phenolic content, ethyl acetate young leaves extract had the highest flavonoid<br />content, and the highest carotenoid content was given by n-hexane root extract. There was significantly negative correlation between TPC in root<br />extract of kelakai with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP.<br />Conclusions: All different extracts of kelakai parts were categorized as very strong antioxidants by DPPH method. Phenolic compounds in kelakai<br />root extract were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant<br />activities of root kelakai extract.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Stenochlaena palustris, Young leaves, Old leaves, Root.<br />50</p>

scholarly journals Preliminary evaluation of selected Prunus spinosa and P. insititia genotypes for their nutraceutical properties

2011 ◽  
Vol 17 (4-5) ◽  
Author(s):  
N. Papp ◽  
F. Szőke ◽  
Z. Szabó ◽  
J. Nyéki ◽  
É. Stefanovits-Bányai ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Preliminary Evaluation ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Prunus Spinosa ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Fruits of nine Prunus spinosa and P. insititia selections were compared in their ferric reducing antioxidant power and total phenolic content. The antioxidant capacity and total phenolic content ranged between 6.36 and 29.26 mmol AA/L, and 5.04 and 29.71 mmol GA/L, respectively. These ranges cover an almost 5-times variation among the tested genotypes. The Pearson’s coefficient was very high (0.92) indicating a major contribution of polyphenolics to the antioxidant capacity of the tested Prunus fruits. Conserving resulted in an approximate 20 % loss of antioxidant power and slightly increased phenolic contents. Our results led us to the conclusion that fruit of Prunus spinosa and P. insititia might be considered as rich sources of antioxidants. In addition, procession with heat treatment caused only a slight decrease in the antioxidant capacity without loss in the total polyphenolic content.

scholarly journals Antioxidant Potential of Grass Pea Seeds from European Countries

Foods ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 142 ◽  
Author(s):  
Wojciech Rybiński ◽  
Magdalena Karamać ◽  
Katarzyna Sulewska ◽  
Andreas Börner ◽  
Ryszard Amarowicz
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolics ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Grass Pea ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
Derivatives Of

Phenolic compounds were extracted from seeds of 30 varieties of grass pea (Lathyrus sativus) into 80% (v/v) methanol. The total phenolics compounds content of the extracts and their antioxidant activity were determined using Folin-Ciocalteu’s phenol reagent and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric-reducing antioxidant power (FRAP) methods, respectively. Total phenolic contents ranged from 1.88 to 7.12 mg/g extract and 20.3 to 70.3 mg/100 g seeds. The extracts and seeds were characterized using Trolox equivalent antioxidant capacity values of 0.015–0.037 mmol Trolox/g extract and 0.158–0.372 mmol Trolox/100 g seeds, and FRAP values of 0.045–0.120 mmol Fe2+/g extract and 0.487–1.189 Fe2+/100 g seeds. The total phenolics content of grass pea extract was correlated with the results of the ABTS (r = 0.881) and FRAP (r = 0.781) assays. The same correlation was observed between the results of both assays (r = 0.842). Two derivatives of p-coumaric acid were the dominant phenolic compounds of the Derek cultivar of grass pea.

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