scholarly journals Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

2021 ◽  
Vol 26 (4) ◽  
pp. 254-264
Author(s):  
Nuril Azhar ◽  
Ervia Yudiati ◽  
Subagiyo Subagiyo ◽  
Rabia Alghazeer
Keyword(s):  
Secondary Metabolite ◽  
Vibrio Vulnificus ◽  
Bacterial Pathogen ◽  
Disease Outbreaks ◽  
Inhibition Zone ◽  
Marine Biota ◽  
Genus Streptomyces ◽  
Streptomyces Spp ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi have been found in aquatic environments and suspected as the primary trigger of WFD (White Feces Disease) outbreaks in aquaculture. This Vibrio spp. has an antibiotic resistance to Ampicillin, Co-Amoxiclav, Amoxicillin, Azithromycin, Actinobacteria and Ciprofloxacin HCL. Actinobacteria and alginate have been reported to increase the marine biota resistance against diseases through prebiotic and probiotic mechanisms. This study aims to discover and increase the secondary metabolite production of Actinobacteria-Alginate and its ability as anti-vibrio. Alginate extraction in the samples dated September 2020 originally from Teluk Awur Bays, Jepara, Central Java, Indonesia (33.73±1.84%) was considerably higher than in May 2021 (22.67±0.3%). Samples were taken from sediment and mangrove root. Actinobacteria strains are macroscopically and microscopically similar to the genus Streptomyces. The most well-known antibiotics were produced by Streptomyces spp. The anti-vibrio test was carried out by Kirby-Bauer disc diffusion. The results were observed by measuring the inhibition zone surrounding the paper disc using a digital calliper. Co-culture strain 90 together with alginate have an approved antibacterial activity against all Vibrio spp. in the concentration of 10.disc-1 mg and 5 mg.disc-1. Co-culture Actinobacteria with alginate has remarkably changed the green-yellow color to olive green/dark red-orange (strains 3, 62, 63, 72, and 90), indicating the transformation of the formation alginate with pigments into other compounds through the biosynthetic pathway. Therefore, alginate enables to support of Actinobacteria by induction the active secondary metabolite as an anti-vibrio to counteract the bacterial pathogen diseases.

scholarly journals Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

2020 ◽  
Vol 23 (3) ◽  
pp. 381-387
Author(s):  
Subagiyo Subagiyo ◽  
Ervia Yudiati ◽  
Nuril Azhar ◽  
Rabia Alghazeer
Keyword(s):  
Active Compound ◽  
Vibrio Parahaemolyticus ◽  
Inhibition Zone ◽  
Vibrio Species ◽  
Disk Diffusion ◽  
Shrimp Pond ◽  
Pine Oil ◽  
Pathogenic Vibrio ◽  
Agar Media ◽  
Vibrio Spp

Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and  V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively.  Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol  4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth. 

scholarly journals Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Iman Rusmana ◽  
ISRAMILDA ISRAMILDA ◽  
Alina Akhdiya
Keyword(s):  
Vibrio Vulnificus ◽  
Vibrio Harveyi ◽  
Pond Water ◽  
Bacillus Sp ◽  
Shrimp Ponds ◽  
Pathogenic Vibrio ◽  
Bacillus Spp ◽  
Ph Range ◽  
Vibrio Spp ◽  
Filtration Technique

Abstract. Rusmana I, Isramilda, Akhdiya A. 2021. Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds. Biodiversitas 22: 4872-4879. Pathogenic Vibrio spp. such as Vibrio vulnificus, V. alginolyticus, V. fluvialis, V. anguillarum, and V. harveyi caused shrimp diseases. The application of bacterial probiotics can control the growth of pathogenic Vibrio in shrimp. Bacillus spp. can produce antimicrobial compounds that inhibit the growth of pathogenic Vibrio spp. Isolation of Bacillus from several pond water samples, pond sediment, and shrimp intestines was successfully collected 175 isolates of Bacillus spp. Three isolates, i.e., Ltw54, Lts36, dan Lts40 had a high inhibitory index and stability in inhibiting the growth of Vibrio harveyi. Verification assay showed that Bacillus sp. Lts40 had the highest antimicrobial activity. The competition assay showed that Bacillus sp Lts40 isolates inhibited the growth of V. harveyi up to 81,8%. The antimicrobial compound produced by Bacillus sp. Lts40 was stable at the pH range of 3-11 and remained stable after heating at 100ºC for 20 minutes. The purification results using the chromatographic filtration technique showed that the protein fraction with a molecular weight of 47,38 kDa effectively inhibited V. harveyi growth. Bacillus sp. Lts40 isolate has potential application as a probiotic agent in shrimp ponds to control the growth of V. harveyi that causes luminous vibriosis diseases and the antimicrobial substance is potentially to be developed and produced as an anti-V. harveyi product that can be applied in a shrimp hatchery.

Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

2011 ◽  
Vol 25 (4) ◽  
pp. 174-176 ◽  
Author(s):  
Hidemasa Izumiya ◽  
Kazutoshi Matsumoto ◽  
Shunsuke Yahiro ◽  
Jiyoung Lee ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

scholarly journals Antibiotic Resistance Analysis of Vibrio spp Isolated from Different Types of Water Sources of Bangladesh and Their Characterization

2019 ◽  
pp. 19-29 ◽  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Diarrheal Disease ◽  
Vibrio Vulnificus ◽  
Culture Media ◽  
Current Knowledge ◽  
Water Sources ◽  
Biomedical Sciences ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Cholera is an acute form of the diarrheal disease that plagued human civilization over the centuries. The enormity of human sufferings led clinicians and scientists to carry out extensive research on cholera and Vibrio cholerae leading to major discoveries that opened up novel areas of research or new disciplines in biomedical sciences. An attempt is made here to summarize some of these breakthroughs and outline their significance in broader perspectives. In the present study, a total of 12 samples were collected from four types of water sources for isolation of Vibrio spp. Water samples are enrichment into alkaline peptone water then inoculate into culture media such as Nutrient agar, MacConkey, and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar medium. After incubation of TCBS plates for 24h at 37°C yellow and green colonies are screened out for biochemical identification. No-sucrose fermenting Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus show green colonies and sucrose fermenting Vibrio cholerae and Vibrio alginolyticus show yellow colonies on TCBS. To identify the Vibrio spp isolates biochemical test was carried out and typical Vibrio spp give a positive result. The majority of Vibrio spp are avirulent, but certain strains may be sporadically human pathogenic. The antibiotic resistance studies showed that among the 9 isolates were resistant against Erythromycin, Penicillin, Cephalexin, Vancomycin and 4 isolates resistant against Tetracycline. To expand current knowledge of the occurrence, ecological niche and persistence of potential human pathogenic Vibrio spp in aquatic environments, occurrence, and laboratory studies were performed.

scholarly journals Increases in the Amounts of Vibrio spp. in Oysters upon Addition of Exogenous Bacteria

2013 ◽  
Vol 79 (17) ◽  
pp. 5208-5213 ◽  
Author(s):  
Brett Froelich ◽  
James Oliver
Keyword(s):  
Coastal Waters ◽  
Vibrio Vulnificus ◽  
Bacterial Pathogen ◽  
Stable Population ◽  
Filter Feeding ◽  
Content Type ◽  
Exogenous Bacteria ◽  
Vibrio Spp

ABSTRACTThe bacterial pathogenVibrio vulnificusis found naturally in brackish coastal waters but can be greatly concentrated by filter-feeding organisms such as shellfish. Numerous experiments in which exogenousV. vulnificuscells are added to oysters in an attempt to measure uptake and depuration have been performed. In nearly all cases, results have shown that laboratory-grown bacteria are rapidly taken up by the oysters but ultimately eliminated, while naturally presentVibriopopulations in oysters are resistant to depuration. In this study, oysters harvested during winter months, with low culturableVibrioconcentrations, were incubated in aquaria supplemented with strains ofV. vulnificusthat were either genotypically or phenotypically distinct from the background bacteria. These exogenous cells were eliminated from the oysters, as previously seen, but other vibrios already inhabiting the oysters responded to theV. vulnificusinoculum by rapidly increasing in number and maintaining a large stable population. The presence of such an oyster-adaptedVibriopopulation would be expected to prevent colonization by exogenousV. vulnificuscells, thus explaining the rapid depuration of these added bacteria.

Effectiveness of Icing as a Postharvest Treatment for Control of Vibrio vulnificus and Vibrio parahaemolyticus in the Eastern Oyster (Crassostrea virginica)

2008 ◽  
Vol 71 (7) ◽  
pp. 1475-1480 ◽  
Author(s):  
KEVIN MELODY ◽  
RESHANI SENEVIRATHNE ◽  
MARLENE JANES ◽  
LEE ANN JAYKUS ◽  
JOHN SUPAN
Keyword(s):  
Crassostrea Virginica ◽  
Cold Storage ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Storage Period ◽  
Eastern Oyster ◽  
Pathogenic Vibrio ◽  
Postharvest Treatment ◽  
Shellfish Sanitation ◽  
Vibrio Spp

The focus of this research was to investigate the efficacy of icing as a postharvest treatment for reduction of the levels of Vibrio vulnificus and Vibrio parahaemolyticus in commercial quantities of shellstock oysters. The experiments were conducted in June and August of 2006 and consisted of the following treatments: (i) on-board icing immediately after harvest; (ii) dockside icing approximately 1 to 2 h prior to shipment; and (iii) no icing (control). Changes in the levels of pathogenic Vibrio spp. during wholesale and retail handling for 2 weeks postharvest were also monitored. On-board icing achieved temperature reductions in all sacks in accordance with the National Shellfish Sanitation Program standard, but dockside icing did not meet this standard. Based on one-way analysis of variance, the only statistically significant relationship between Vibrio levels and treatment occurred for samples harvested in August; in this case, the levels of V. vulnificus in the noniced oysters were significantly higher (P < 0.05) than were the levels in the samples iced on-board. When analyzing counts over the 14-day storage period, using factorial analysis, there were statistically significant differences in V. vulnificus and V. parahaemolyticus levels by sample date and/or treatment (P < 0.05), but these relationships were not consistent. Treated (iced) oysters had significantly higher gaping (approximately 20%) after 1 week in cold storage than did noniced oysters (approximately 10%) and gaping increased significantly by day 14 of commercial storage. On-board and dockside icing did not predictably reduce the levels of V. vulnificus or V. parahaemolyticus in oysters, and icing negatively impacted oyster survival during subsequent cold storage.

scholarly journals Complete Genome Sequence of the Pathogenic Vibrio vulnificus Type Strain ATCC 27562

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Douglas B. Rusch ◽  
Dean A. Rowe-Magnus
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Vibrio Vulnificus ◽  
Foodborne Pathogen ◽  
The United States ◽  
Environmental Isolates ◽  
Content Type ◽  
Pathogenic Vibrio

ABSTRACT Vibrio vulnificus has the highest death rate and economic burden per case of any foodborne pathogen in the United States. A complete genome sequence of the type strain promotes comparative analyses with other clinical and environmental isolates, improving our understanding of this important human pathogen and successful environmental organism.

scholarly journals Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

2021 ◽  
Vol 869 (1) ◽  
pp. 012033
Author(s):  
D R Utami ◽  
I Irwan ◽  
S Agustina ◽  
S Karina ◽  
S Afriani
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Hexane Extract ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Hexane Extracts ◽  
Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

scholarly journals ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO

2005 ◽  
Vol 7 (1) ◽  
pp. 80
Author(s):  
Kamiso Handoyo Nitimulyo ◽  
Alim Isnansetyo ◽  
Triyanto Triyanto ◽  
Indah Istiqomah ◽  
Muhammmad Murdjani
Keyword(s):  
Intraperitoneal Injection ◽  
The Body ◽  
Total Length ◽  
Specific Disease ◽  
Pathogenic Vibrio ◽  
Causative Agents ◽  
Development Center ◽  
Aquaculture Development ◽  
Vibrio Spp

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.

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