scholarly journals Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

2020 ◽  
Vol 23 (3) ◽  
pp. 381-387
Author(s):  
Subagiyo Subagiyo ◽  
Ervia Yudiati ◽  
Nuril Azhar ◽  
Rabia Alghazeer
Keyword(s):  
Active Compound ◽  
Vibrio Parahaemolyticus ◽  
Inhibition Zone ◽  
Vibrio Species ◽  
Disk Diffusion ◽  
Shrimp Pond ◽  
Pine Oil ◽  
Pathogenic Vibrio ◽  
Agar Media ◽  
Vibrio Spp

Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and  V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively.  Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol  4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth. 

scholarly journals Isolation and selection of bacteria against shrimp pathogenic Vibrio parahaemolyticus from shrimp pond water on Duyen Hai district, Tra Vinh province

2020 ◽  
Vol 10 (1) ◽  
pp. 43-52
Author(s):  
Tran Vu Phuong ◽  
Dang Thi Ngoc Thanh ◽  
Cao Ngoc Diep
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Pond Water ◽  
Shrimp Farming ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Shrimp Pond ◽  
Pathogenic Vibrio ◽  
The Status

Antibiotic has frequently been used in the shrimp-farming process in Vietnam. This leads to the status that antibiotic-resistant bacteria and products do not receive in the market. Bacteria had the resistant ability to pathogenic bacteria in water, and they have an important role in sustainable aquaculture. This study aimed to isolate and select good bacterial strains against Vibrio parahaemolyticus, pathogenic bacteria, on shrimp from 8 samples of shrimp pond water at 3 villages Ngu Lac, Phuoc An and Long Toan of Duyen Hai district, Tra Vinh province on NB agar medium. As a result, fifty-nine bacterial isolates were isolated and 10/59 isolates (16.95%) were identified as resistant to Vibrio parahaemolyticus by the well diffusion method. In 10 isolates, there were 7 isolates had good resistance to select for PCR technique and sequencing. The result indicated that these seven strains, including DH1m, DH2f, DH4d, DH8i, DH8m, DH8n, belonged to Bacilli and DH1n strain belonged to Streptomyces sp.

scholarly journals The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

2020 ◽  
Vol 17 (18) ◽  
pp. 6781
Author(s):  
Qingyao Wang ◽  
Songzhe Fu ◽  
Qian Yang ◽  
Jingwei Hao ◽  
Can Zhou ◽  
...  
Keyword(s):  
Seawater Intrusion ◽  
Water Area ◽  
Vibrio Species ◽  
Most Probable Number ◽  
Potential Health ◽  
Probable Number ◽  
Inland River ◽  
Pathogenic Vibrio ◽  
The Impact ◽  
Vibrio Spp

The estuary is the ecological niche of pathogenic Vibrio spp. as it provides abundant organic and inorganic nutrients from seawater and rivers. However, little is known about the ecology of these Vibrio species in the inland brackish water area. In this study, their co-occurrence and relationships to key environmental constraints (salinity and temperature) in the Hun-Tai River of China were examined using the most probable number polymerase chain reaction (MPN-PCR) approach. We hereby report 2-year continuous surveillance based on six water indices of the Hun-Tai River. The results showed that seawater intrusion maximally reached inland as far as 26.5 km for the Hun-Tai River. Pathogenic Vibrio spp. were detected in 21.9% of the water samples. In particular, V. cholerae, V. parahaemolyticus, and V. vulnificus were isolated in 10 (10.4%), 20 (20.8.5%), and 2 (2.08%) samples, respectively. All V. parahaemolyticus strains were tdh gene negative, 10% were positive for the trh gene. Multi-locus sequence typing (MLST) divided V. parahaemolyticus strains into 12 sequence types (STs) for the Hun-Tai River. Five STs were respectively present in various locations along the Hun-Tai River. The PCR assay for detecting six virulence genes and Vibrio seventh pandemic island I and II revealed three genotypes in 12 V. cholerae isolates. The results of our study showed that seawater intrusion and salinity have profound effects on the distribution of pathogenic Vibrio spp. in the inland river, suggesting a potential health risk associated with the waters of the Hun-Tai River used for irrigation and drinking.

scholarly journals Comparative Genomics of Clinical and Environmental Isolates of Vibrio spp. of Colombia: Implications of Traits Associated with Virulence and Resistance

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1605
Author(s):  
Alejandra Pérez-Duque ◽  
Andrea Gonzalez-Muñoz ◽  
Jorge Arboleda-Valencia ◽  
Lizbeth Janet Vivas-Aguas ◽  
Tania Córdoba-Meza ◽  
...  
Keyword(s):  
Virulence Gene ◽  
Vibrio Species ◽  
Clinical Samples ◽  
Surveillance Program ◽  
Genomic Information ◽  
Environmental Isolates ◽  
Important Species ◽  
Pathogenic Vibrio ◽  
Routine Surveillance ◽  
Vibrio Spp

There is widespread concern about the increase in cases of human and animal infections caused by pathogenic Vibrio species due to the emergence of epidemic lineages. In Colombia, active surveillance by the National Institute of Health (INS) has confirmed the presence of Vibrio; however, in routine surveillance, these isolates are not genomically characterized. This study focused on the pangenome analysis of six Vibrio species: V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. fluvialis, V. diabolicus and V. furnissii to determine the genetic architectures of potentially virulent and antimicrobial resistance traits. Isolates from environmental and clinical samples were genome sequenced, assembled and annotated. The most important species in public health were further characterized by multilocus sequence typing and phylogenomics. For V. parahaemolyticus, we found the virulent ST3 and ST120 genotypes. For V. vulnificus, we identified isolates belonging to lineages 1 and 2. Virulence gene homologues between species were found even in non-pathogenic species such as V. diabolicus. Annotations related to the mobilome, integrative mobile and conjugative elements and resistance genes were obtained from environmental and clinical isolates. This study contributes genomic information to the intensified surveillance program implemented by the INS to establish potential sources of vibriosis in Colombia.

scholarly journals Vibrio Species in an Urban Tropical Estuary: Antimicrobial Susceptibility, Interaction with Environmental Parameters, and Possible Public Health Outcomes

2021 ◽  
Vol 9 (5) ◽  
pp. 1007
Author(s):  
Anna L. B. Canellas ◽  
Isabelle R. Lopes ◽  
Marianne P. Mello ◽  
Rodolfo Paranhos ◽  
Bruno F. R. de Oliveira ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Environmental Parameters ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Vibrio Species ◽  
Beta Lactam ◽  
Pathogenic Vibrio ◽  
Extended Spectrum Beta Lactamases ◽  
Nitrogen Concentrations ◽  
Vibrio Spp

The genus Vibrio comprises pathogens ubiquitous to marine environments. This study evaluated the cultivable Vibrio community in the Guanabara Bay (GB), a recreational, yet heavily polluted estuary in Rio de Janeiro, Brazil. Over one year, 66 water samples from three locations along a pollution gradient were investigated. Isolates were identified by MALDI-TOF mass spectrometry, revealing 20 Vibrio species, including several potential pathogens. Antimicrobial susceptibility testing confirmed resistance to aminoglycosides, beta-lactams (including carbapenems and third-generation cephalosporins), fluoroquinolones, sulfonamides, and tetracyclines. Four strains were producers of extended-spectrum beta-lactamases (ESBL), all of which carried beta-lactam and heavy metal resistance genes. The toxR gene was detected in all V. parahaemolyticus strains, although none carried the tdh or trh genes. Higher bacterial isolation rates occurred in months marked by higher water temperatures, lower salinities, and lower phosphorus and nitrogen concentrations. The presence of non-susceptible Vibrio spp. was related to indicators of eutrophication and sewage inflow. DNA fingerprinting analyses revealed that V. harveyi and V. parahaemolyticus strains non-susceptible to antimicrobials might persist in these waters throughout the year. Our findings indicate the presence of antimicrobial-resistant and potentially pathogenic Vibrio spp. in a recreational environment, raising concerns about the possible risks of human exposure to these waters.

scholarly journals Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

2021 ◽  
Vol 26 (4) ◽  
pp. 254-264
Author(s):  
Nuril Azhar ◽  
Ervia Yudiati ◽  
Subagiyo Subagiyo ◽  
Rabia Alghazeer
Keyword(s):  
Secondary Metabolite ◽  
Vibrio Vulnificus ◽  
Bacterial Pathogen ◽  
Disease Outbreaks ◽  
Inhibition Zone ◽  
Marine Biota ◽  
Genus Streptomyces ◽  
Streptomyces Spp ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi have been found in aquatic environments and suspected as the primary trigger of WFD (White Feces Disease) outbreaks in aquaculture. This Vibrio spp. has an antibiotic resistance to Ampicillin, Co-Amoxiclav, Amoxicillin, Azithromycin, Actinobacteria and Ciprofloxacin HCL. Actinobacteria and alginate have been reported to increase the marine biota resistance against diseases through prebiotic and probiotic mechanisms. This study aims to discover and increase the secondary metabolite production of Actinobacteria-Alginate and its ability as anti-vibrio. Alginate extraction in the samples dated September 2020 originally from Teluk Awur Bays, Jepara, Central Java, Indonesia (33.73±1.84%) was considerably higher than in May 2021 (22.67±0.3%). Samples were taken from sediment and mangrove root. Actinobacteria strains are macroscopically and microscopically similar to the genus Streptomyces. The most well-known antibiotics were produced by Streptomyces spp. The anti-vibrio test was carried out by Kirby-Bauer disc diffusion. The results were observed by measuring the inhibition zone surrounding the paper disc using a digital calliper. Co-culture strain 90 together with alginate have an approved antibacterial activity against all Vibrio spp. in the concentration of 10.disc-1 mg and 5 mg.disc-1. Co-culture Actinobacteria with alginate has remarkably changed the green-yellow color to olive green/dark red-orange (strains 3, 62, 63, 72, and 90), indicating the transformation of the formation alginate with pigments into other compounds through the biosynthetic pathway. Therefore, alginate enables to support of Actinobacteria by induction the active secondary metabolite as an anti-vibrio to counteract the bacterial pathogen diseases.

Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

2011 ◽  
Vol 25 (4) ◽  
pp. 174-176 ◽  
Author(s):  
Hidemasa Izumiya ◽  
Kazutoshi Matsumoto ◽  
Shunsuke Yahiro ◽  
Jiyoung Lee ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Pathogenic Vibrio Species in Dutch Shellfish Destined for Direct Human Consumption

2010 ◽  
Vol 73 (4) ◽  
pp. 734-738 ◽  
Author(s):  
FRANCISKA M. SCHETS ◽  
HAROLD H. J. L. van den BERG ◽  
SASKIA A. RUTJES ◽  
ANA MARIA de RODA HUSMAN
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Vibrio Species ◽  
Most Probable Number ◽  
Human Consumption ◽  
Gastrointestinal Infections ◽  
Probable Number ◽  
The North ◽  
Pathogenic Vibrio ◽  
The North Sea ◽  
Production Areas

Vibrio parahaemolyticus is a common cause of shellfish-related gastroenteritis all over the world. V. parahaemolyticus and Vibrio alginolyticus have previously been detected in water samples from the Oosterschelde, a large inlet on the North Sea, which is used for both recreational purposes and shellfish production. In 2006, oysters (Crassostrea gigas) from a noncommercial oyster bed in the Oosterschelde and oysters bought in Dutch fish shops were tested for the presence of pathogenic Vibrio species; in 2007 and 2008, oysters (C. gigas) and mussels (Mytilus edulis) from Oosterschelde production areas were examined. Total Vibrio numbers were related to water temperatures to study joint patterns. Vibrio was found in oysters and mussels from the production areas, and levels ranged from 6 to 622 most probable number (MPN) per g in oysters and 6 to 62 MPN/g in mussels. Vibrio levels in oysters from fish shops were 231 to >333 MPN/g, whereas levels in noncommercial oysters ranged from 231 to >2,398 MPN/g. About 80% of the isolated strains were V. alginolyticus, and approximately 10% were identified as V. parahaemolyticus. Vibrio counts in shellfish samples increased with increasing water temperature and declined when water temperatures dropped; Vibrio was not detected when water temperatures declined to <13.5°C. Based on the obtained results and the known high V. parahaemolyticus dose (<104 cells per serving of oysters) required for infection, it is concluded that the risk of gastrointestinal infections with V. parahaemolyticus through consumption of shellfish from the Oosterschelde production sites is presumably low.

scholarly journals Prevalence, antimicrobial resistance profiles and virulence genes of Vibrio spp. isolated from shrimp retails in Ho Chi Minh City (Vietnam)

2019 ◽  
Vol 18 (03) ◽  
pp. 27-34
Author(s):  
Trinh N. T. Huynh
Keyword(s):  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Common Species ◽  
Vibrio Species ◽  
Ho Chi Minh City ◽  
Pathogenic Vibrio ◽  
Food Borne ◽  
Food Quality And Safety ◽  
Vibrio Spp

This study was conducted to determine the diversity of pathogenic Vibrio species, the antimicrobial resistance profile and the presence of virulence genes linked to food-borne pathogens of Vibrio spp. isolated from shrimp samples in Ho Chi Minh City, Vietnam. A total of 40 raw shrimp batches were collected from retails markets (supermarket and street). All 133 test strains were isolated from 40 shrimp samples. V. parahaemolyticus was the most common species (87.5%), followed by V. navarrensis (60%), V. alginolyticus (52.5%), V. cholerae non-O1 (37.5%), V. vulnificus (22.5%), and V. fluvialis (10%). Vibrio spp. isolates were susceptible to 12 antimicrobial agents. The prevalence of ampicillin resistance was highest (82.7%), followed by cotrimoxazole (18.8%) and 3rd generation cephalosporins (16.5% cefotaxime and 8.3% ceftazidime). Extended spectrum β lactamase (ESBL) activity was detected in 28.1% V. parahaemolyticus isolates. None of tdh or trh virulence genes were detected. The results of this study indicated the presentation of Vibrio species in shrimp samples purchased in Ho Chi Minh City. Therefore, our results could be of great potential for the identification of Vibrio infection in shrimp samples taken from different regions to improve food quality and safety.

scholarly journals Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp.

1988 ◽  
Vol 1 (3) ◽  
pp. 245-267 ◽  
Author(s):  
J M Janda ◽  
C Powers ◽  
R G Bryant ◽  
S L Abbott
Keyword(s):  
Clinical History ◽  
Vibrio Species ◽  
Human Pathogens ◽  
Isolation And Identification ◽  
Virulence Potential ◽  
Pathogenic Vibrio ◽  
Common Clinical Presentation ◽  
Vibrio Cholerae O1 ◽  
Clinically Significant ◽  
Vibrio Spp

Recent taxonomic advances have now implicated several different Vibrio species as human pathogens. While the most common clinical presentation of Vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. Detection of Vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to Vibrio species. Except for Vibrio cholerae O1 and Vibrio parahaemolyticus, there is little direct evidence linking the production of a myriad of cell-associated or extracellular factors produced by each species with human disease and pathogenesis. Many questions regarding pathogenic Vibrio species remain unanswered, including their frequency and distribution in environmental specimens (water, shellfish), infective doses, virulence potential of individual isolates, and markers associated with such strains.

scholarly journals Surveillance of Vibrio spp in Penaeus monodon collected from shrimp pond of Satkhira, Bangladesh

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Abdullah Al-Amin ◽  
Shahena Aktar Shipa ◽  
M. Niamul Naser ◽  
Md. Faruque Miah
Keyword(s):  
Penaeus Monodon ◽  
Vibrio Species ◽  
Economic Losses ◽  
Biochemical Tests ◽  
Shrimp Pond ◽  
Tiger Shrimp ◽  
Food Borne ◽  
Physiological And Biochemical ◽  
Vibrio Spp

Vibrio is the most common genera associated with crustaceans and often causing significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was conducted to identify Vibrio spp. from the tiger shrimp (Penaeus monodon) of shrimp pond at Satkhira, Bangladesh. A total number of 33 Vibrio species isolates were identified from 20 shrimp samples through a series of morphological, physiological and biochemical tests. The work reports the prevalence of Vibrio spp. in the pond environments and the existence of three Vibrio species such as V. alginolyticus, V. parahaemolyticus and V. harveyi were identified. In the study of antibiogram, all isolates were shown 100% sensitive to streptomycin, ciprofloxacin and chloramphenicol. Maximum 41% isolates were shown resistant to co-trimethaxozole whereas 30% and 24% resistant to azithromycin and novobiocin respectively.

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