scholarly journals Pertumbuhan vegetatif hasil split Dendrobium (Dendrobium sp.) pada dua jenis pupuk nitrogen dan tempat tanam

2019 ◽  
Vol 3 (1) ◽  
pp. 65
Author(s):  
Indah Suci Pratiwi ◽  
Endang Dwi Purbajanti ◽  
Eny Fuskhah
Keyword(s):  
Plant Height ◽  
Nitrogen Fertilizer ◽  
Vegetative Growth ◽  
Ornamental Plants ◽  
Stem Diameter ◽  
Coconut Fiber ◽  
Azolla Microphylla ◽  
Synthetic Fertilizer ◽  
Split Plot ◽  
Main Plot

ABSTRACT Orchids are ornamental plants that lived in the wild as epiphytes, utilized the visual side of the flower. Problems arise is a relatively long time to be able to enjoy the flowers. Split technique is an alternative that produces special orchids especially of Dendrobium type with the same properties as its mother in a relatively short time. The research was conducted in July-September 2017 in screenhouse, Faculty of Animal and Agricultural Sciences, Diponegoro University. This study aimed to know the interaction between type of nitrogen fertilizer and place of planting to vegetative growth of split Dendrobium sp. This study used Split Plot Design of Complete Randomized Design scheme with ten replications. The main plot was place of planting : T1 = attached to the coconut fiber then hung and T2 = grown in pot (coconut fiber) and subplot was the type of fertilizer: P1 = POC goat + Azolla microphylla, and P2 = Synthetic fertilizer. The variables observed were plant height, number of leaves, stem diameter, number of roots, and number of buds per plant. Data were analyzed by analysis of variance followed by LSD test at 5% level. The results showed that hook planting places significantly increased plant height and stem diameter. Synthetic fertilizer significantly increased plant height and number of leaves.There was interaction between nitrogen fertilizer and place of planting to stem diameter on vegetative growth results of split Dendrobium (Dendrobium sp.). Keywords: Dendrobium sp., nitrogen fertilizer, place of planting ABSTRAK  Anggrek merupakan tanaman hias yang hidup di alam bebas secara epifit, dimanfaatkan sisi visualnya terutama dari bentuk dan warna bunga. Masalah yang timbul adalah waktunya yang relatif lama untuk berbunga. Teknik split merupakan alternatif yang menghasilkan anggrek khususnya jenis Dendrobium dengan sifat sama seperti induknya dalam waktu yang relatif singkat. Penelitian dilaksanakan pada Juli-September 2017 di screen house, Departemen Pertanian, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Tujuan penelitian adalah untuk mengetahui pengaruh interaksi jenis pupuk dan tempat tanam terhadap pertumbuhan vegetatif hasil split Dendrobium sp. Penelitian ini menggunakan Rancangan Petak Terbagi (Split Plot) skema RAL dengan 10 kali ulangan. Petak utama (main plot) adalah tempat tanam: T1 = dilekatkan pada serabut kelapa kemudian digantung dan T2 = ditanam dalam pot (serabut kelapa) dan anak petak (sub plot) adalah jenis pupuk: P1 = POC kotoran kambing+Azolla microphylla, dan P2 = pupuk sintetis. Variabel yang diamati antara lain adalah tinggi tanaman, jumlah daun, diameter batang, jumlah akar, dan jumlah tunas per tanaman. Data yang diperoleh dianalisis dengan ANOVA dan dilanjutkan dengan uji BNT (Beda Nyata Terkecil) pada taraf 5%. Hasil penelitian menunjukkan bahwa tempat tanam gantungan meningkatkan tinggi tanaman dan diameter batang secara signifikan. Pupuk sintetis meningkatkan tinggi tanaman dan jumlah daun secara signifikan. Terjadi interaksi antara pemberian pupuk nitrogen dan tempat tanam terhadap diameter batang pada pertumbuhan vegetatif hasil split Dendrobium (Dendrobium sp.).

scholarly journals Çeşitli Biyoaktivatör Uygulamalarının Çarkıfelek ve Guava Bitkilerinin Fidan Gelişimi Üzerine Etkileri

2020 ◽  
Vol 8 (2) ◽  
pp. 421
Author(s):  
Canan Nilay Duran ◽  
Gizem Demirkaplan ◽  
Sevinç Şener
Keyword(s):  
Plant Height ◽  
Growth And Development ◽  
Ornamental Plants ◽  
Nutrient Content ◽  
Psidium Guajava ◽  
Stem Diameter ◽  
Sapling Growth ◽  
Number Of Leaves ◽  
Average Plant ◽  
Average Plant Height

Passionflower is a member of Passifloraceae family, it can be used as medicinal and ornamental plants in addition to its consumption as fruit in the world. Guava (Psidium guajava L.) fruits, which has a rich nutrient content, are consumed both fresh and processed. Passionfruit and guava plants, which can only be cultivated in temperate southern coasts in our country, have gained commercial importance because of the fruits' export potential, high medical importance and nutrient. This study was carried out to determine the effects of some bioactivator applications on the criteria of sapling growth and development of passionflower and guava plants. The experiment was conducted between 2018-2019 under greenhouse conditions. Saplings obtained from seed germination in Akdeniz University Faculty of Agriculture Research and Experiment Area were used as plant material. 3 different commercial preparations called Messenger, Crop-set and ISR-2000 were used as bioactivators. Plant height (cm), stem diameter (mm) and number of leaves (number / plant) were measured weekly, in order to determine the effect of the applications on the growth and development of saplings. At the end of the study, it was determined that the highest average plant height (10.17 cm), stem diameter (13.53 mm) were obtained in ISR-2000 application. The highest average plant height (11.93 cm), stem diameter (16.44 mm) and number of leaves (9.07 units / plant) were obtained from Messenger application in guava plant. When the results obtained are evaluated, it is recommended that ISR-2000 bioactivator can be applied in passionflower sapling cultivation and Messenger bioactivator can be applied for guava sapling cultivation.

scholarly journals Pengaruh Pemberian Zeolite dan Dosis Pupuk Nitrogen Terhadap Pertumbuhan Vegetatif Jagung (Zea mays, L.) di Media Pasir Pantai

2021 ◽  
Vol 1 (1) ◽  
pp. 39-43
Author(s):  
Gunawan Budiyanto ◽  
Mulyono Mulyono ◽  
Fiyoni Dwi Setyawan
Keyword(s):  
Plant Height ◽  
Sandy Soil ◽  
Nitrogen Fertilizer ◽  
Vegetative Growth ◽  
Dry Weight ◽  
Maximum Growth ◽  
Fertilizer Application ◽  
Research Field ◽  
Maize Crop ◽  
Randomized Design

A research to study the effects of Zeolite and nitrogen fertilizer application on vegetative growth of maize crop in coastal sandy soil was conducted on the research field of Agriculture Faculty, Muhammadiyah University of Yogyakarta at Tamantirto, Bantul District of Yogyakarta during October until February, 2003. This research was aimed on the study of Zeolite application in order to enhance the nitrogen uptake in the coastal sandy soil, and its effects on vegetative growth of CP I variety of maize. The pot experiment was arranged in 7 x 3 factorial completely randomized design, with 4 replications. The first factor was Zeolite dosage consisted of 7 levels, i.e:  0, 3, 4, 5, 6, 7, and 8 %; and the second one was 3 levels nitrogen fertilizer dosage which consisted of: 75, 110, and 145 kg/ ha. The nitrogen fertilizer was applied on the medium mixed with Zeolite granules. Observations on plant height, stem diemeter, leaves number, plant fresh and dry weight were done during the vegetative growth of maize. The results showed that Zeolite application significantly enhanced the vegetative growth of CPI maize. The 5,2  - 5,6  % Zeolite was optimum to gave the maximum growth of maize crop. Nitrogen fertilizer application was also significantly increased the plant height and biomass weight, and the 145 kg/ha was the optimum dosage. There was no interaction between Zeolite and nitrogen fertilizer application in order to affected the maize growth.

scholarly journals Pengaruh Pupuk Hayati Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) pada Pembibitan Awal

2019 ◽  
Vol 5 (2) ◽  
pp. 131
Author(s):  
Muh Yusuf Indris ◽  
Irwan Halid ◽  
Sukriming Sapareng
Keyword(s):  
Plant Height ◽  
Nitrogen Fertilizer ◽  
Oil Palm ◽  
Standard Dose ◽  
Organic Fertilizer ◽  
N Fertilizer ◽  
Stem Diameter ◽  
Total N ◽  
Nutrient Levels ◽  
Four Levels

This study aims to determine the effectiveness of N2-fixing microbes contained in organic organic fertilizers at four levels of nitrogen fertilizer application in increasing the performance of oil palm seedlings. The experiment was carried out in the form of an experiment using a Randomized Block Design with 2 factors: organic organic fertilizer and nitrogen fertilizer. The first factor with three levels of treatment, namely without organic biological fertilizer, Organic fertilizer from cow dung (Agro Flower) 1 g / kg of Soil and Organic organic fertilizer (Biost) 1 g / kg of Soil. While the second factor consists of four levels, namely without N fertilizer, N fertilizer 50% of the standard dose, N fertilizer 100% of the standard dose, and N fertilizer of 150% of the standard dose. Each treatment was tested on 4 oil palm seedlings and repeated 3 times, so that 144 experimental plants were obtained. The observations made were the variable plant performance includes plant height, number of leaves, and stem diameter. Variants of plant nutrient levels included total N nutrient levels, P nutrient levels, and K nutrient levels. The results showed that N-inhibiting microbes contained in organic organic fertilizer increased plant height and stem diameter significantly in oil palm nurseries. The use of a 100% dose of N fertilizer together with organic biofertilizers shows the best crop performance results.

scholarly journals Biostimulasi pertumbuhan vegetatif tanaman tebu (Saccharum officinarum L.) pada fase awal di lahan kering (Biostimulation of vegetative growth of sugarcane (Saccharum officinarum L.) in the initial phase on dry land)

2018 ◽  
Vol 86 (2) ◽  
Author(s):  
Sri WAHYUNI ◽  
Hanning Susilo HABIBULLAH ◽  
Soekarno Mismana PUTRA ◽  
Dian Mutiara AMANAH ◽  
. SISWANTO ◽  
...  
Keyword(s):  
Plant Height ◽  
Vegetative Growth ◽  
Control Plant ◽  
Foliar Spray ◽  
Saccharum Officinarum ◽  
Growth Cycle ◽  
Stem Diameter ◽  
Dry Land ◽  
Old Trees ◽  
Sugarcane Stem

AbstractThe expansion of sugarcane areas as a support to national sugar production has shifted to sub-optimal dry land. In drought stress conditions, early growth of sugarcane usually can inhibite and decrease its productivity. This study aimed to test the efficacy of organic biostimulant in increasing vegetative growth of sugarcane in the dry land. Firstly, seedlings were submerged with biostimulant of Citorin-Rfor overnight. Secondly, the biostimulant application of Citorin-S was carried out by foliar sprayat age1 and4 months old trees. Humicacid 0.5% (v/v) was applied in soil before planting while the application of mycorrhiza was carried out by direct pouring on soil during planting. The results showed that the initial vegetative growth of biostimulant-treated sugarcane stem diameter and length were 23% wider and 27% higher compared to that of control, respectively. In subsequent growth cycle, all observed vegetative parameters showed higher growth value in the biostimulant-treated sugarcanes than that of the control. Plant height, stem diameter and number of tillers of biostimulant-treated sugarcanes had significantly higher values than that of the control. P3 treatment (organic biostimulant plus humic acid and mycorrhiza) was the best treatment. The height and diameter of P3 sugarcane stems were 47% wider and 59% higher, respectively, compared to that of control at 107 DAP.[Keywords: biostimulant, plant height, stem diameter, number of tillers, number of leaves] Abstrak Penambahan areal tanaman tebu untuk mendukung peningkatan produksi gula nasional telah bergeser ke areal sub-optimal lahan kering. Pada kondisi cekaman kekeringan, pertumbuhan awal tebu biasanya terhambat dan dapat menurunkan produktivitas saat panen. Penelitian ini bertujuan menguji efikasi biostimulan organik untukmeningkatkan pertumbuhan vegetatif tanaman tebu pada fase awal di lahan kering. Perlakuan biostimulan Citorin-R diaplikasikan pada benih dengan cara perendaman semalam. Perlakuan kedua, biostimulan Citorin-S disemprotkanpada saat tanaman tebu berumur 1 dan 4 bulan secara foliar spray. Aplikasi asam humat 0,5 % (v/v) di tanah dilakukan sebelum tanam, sedangkan aplikasi mikoriza dilakukan dengan pemberian langsung pada tanah saat penanaman bagal tebu. Hasil penelitian menunjukkan bahwa nilai pertumbuhan vegetatif awal tanaman tebu perlakuan memiliki diameter batang sekitar 23% dan tinggi tanaman 27% lebih tinggi daripada tebu kontrol. Pada pertumbuhan selanjutnya, semua parameter vegetatif yang diamati menunjukkan nilai pertumbuhan yang lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Tinggi tanaman, diameter batang dan jumlah anakan secara statistik berbeda nyata lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Perlakuan P3 (biostimulan organik plus asam humat dan mikoriza) adalah perlakuan terbaik. Tinggi dan diameter batang tanaman tebu P3 masing-masing 47% dan 59% lebih besar daripada batang tanaman kontrol pada 107 hari setelah tanam (HST).  [Kata kunci :biostimulan, tinggi tanaman, diameter batang, jumlah anakan, jumlah daun]

scholarly journals The Effectiveness Of Pre-Planting Seeds Bio-Matriconditioning And Application Of Fertilization With Leisa Technique On Growth Of Areca Nut Seedling (Areca Catechu L.)

2021 ◽  
Vol 2 (6) ◽  
pp. 2053-2060
Author(s):  
Gusti Ayu Kade Sutariati ◽  
Muhidin ◽  
Nini Mila Rahni ◽  
Gusti Ngurah Adi Wibawa ◽  
La Mudi
Keyword(s):  
Plant Height ◽  
Dry Weight ◽  
Stem Diameter ◽  
Raw Material ◽  
Inorganic Fertilizer ◽  
Areca Nut ◽  
Randomized Design ◽  
Main Plot ◽  
Wet Weight ◽  
Number Of Leaves

Areca nut is widely used as industrial raw material, both for health and cosmetics and as a source of renewable energy. This study aimed to examine the combination of seed bombatriconditioning and LEISA fertilization treatments that were effective in increasing the growth of areca nut seedlings. The research was carried out in the Wua-Wua Kendari Village, from March to July 2021. The study used a split-plot design with a completely randomized design (CRD). The main plot is seed biomatriconditioning treatment which consists of 3 treatments. While the subplots were fertilized with the LEISA technique which consisted of 6 treatments so that 18 treatment combinations were obtained with 3 replications. Observations were made on plant height, number of leaves, stem diameter, number of roots, wet weight and dry weight of shoot. Observational data were analyzed using analysis of variance, followed by the DMRT α0.05 if there was a significant effect. The results showed that seed biomatriconditioning treatment with rhizobacteria integrated with the LEISA technique was able to increase the growth of areca nut seedlings. The integration between L1R biomatriconditioning and the application of organic plus fertilizer + 100% inorganic fertilizer showed a better growth performance of areca nut on plant height, number of leaves, stem diameter, number of roots, wet weight and dry weight of betel nut which were significantly different with control and application 100% inorganic fertilizer, but not significantly different from organic plus fertilizer, organic plus fertilizer + 50% inorganic fertilizer and organic plus fertilizer + 25% inorganic fertilizer. As a conclusion, pre-planting seed treatment with L1R biomatriconditioning is very important to increase areca nut seed germination. To increase the growth of areca nut seedlings, further fertilization needs to be done with organic plus fertilizer or a combination of organic plus fertilizer + 25% inorganic fertilizer.

scholarly journals Respon pertumbuhan vegetatif semaian akibat aplikasi mikroba potensial pada rehabilitasi pohon kakao tanpa penebangan

Jurnal Agro ◽  
10.15575/9098 ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 235-245
Author(s):  
Marliana S. Palad ◽  
Aminah Aminah
Keyword(s):  
Plant Height ◽  
Stem Diameter ◽  
Trichoderma Asperellum ◽  
Minimum Age ◽  
Main Plot ◽  
Four Levels ◽  
Average Plant ◽  
Old Trees ◽  
Cocoa Tree ◽  
Average Plant Height

Usaha yang dapat dilakukan untuk megatasi penurunan produksi pohon kakao tua dan rusak adalah penerapan inarching grafting atau penyambungan tanaman kakao muda unggul berumur minimal 6 bulan yang ditanam di sekitar pohon tua. Tujuan penelitian ini adalah mengkaji pengaruh aplikasi Trichoderma asperellum dan Azotobacter chroococcum terhadap pertumbuhan vegetatif semaian kakao yang akan disambungkan ke pohon kakao tua menggunakan metode inarching grafting. Penelitian ini menggunakan Rancangan Petak Terbagi dengan tiga ulangan. Petak Utama yaitu aplikasi T.asperellum sebanyak 4 g L-1 setiap tanaman, dengan empat taraf: tanpa T.asperellum (T0), satu kali (T1), dua kali (T2), dan tiga kali (T3) aplikasi. Anak Petak adalah inokulasi A.chroococcum sebanyak 40 ml x 108 cfu setiap tanaman, dengan 3 taraf: tanpa A.chroococcum (A0), satu kali (A1) dan dua kali (A2) aplikasi. Hasil analisis menunjukkan tidak terdapat interaksi antara aplikasi T.asperellum dengan A.chroococcum, tetapi terdapat pengaruh tunggal dari kedua mikroba potensial yang diaplikasikan. Pada umur semaian 90 hst, aplikasi dua kali A.chroococcum menghasilkan rata-rata tinggi tanaman 155,25 cm, total daun 41 helai dan diameter batang 13,10 mm. Pemberian tiga kali T.asperellum menghasilkan rata-rata tinggi tanaman 150,89 cm,  total daun 41,22 helai dan diameter batang 12,86 mm. Semaian yang diberi mikroba potensial digunakan untuk rehabilitasi pohon kakao tua. An efforts that can be done to overcome the decline in production of old and damaged cocoa trees are the application of inarching grafting or grafting of superior young cocoa plants with a minimum age of 6 months planted around old trees. The purpose of this study was to examine the effect of the application of Trichoderma asperellum and Azotobacter chroococcumon the vegetative growth of cocoa seedlings which was grafted to old cocoa trees using the inarching grafting method. The research used a Split Plot Design with three replications. The main plot was application of T.asperellum of 4 g L-1 for each seedling and cocoa tree, consisted of four levels. i.e: without T.asperellum (T0), one time application (T1), two times application (T2), and three times application (T3). The subplot factor was A.chroococcum as much as 40 ml x 108 cfu on each seedling and cocoa tree, consisted of three levels, i.e.: without A. chroococcum (A0), one time application (A1) and two times application (A2).  The analysis of variance results showed that no interaction between applications of T.asperellum  with  A.chroococcum, but it had a singular effect for both potential microbial applied. At 90 days after planting, the application of A.chroococcum twice resulted in an average plant height of 155.25 cm, a total of 41 leaves and a stem diameter of 13.10 mm. Application of T.asperellum three times resulted in an average plant height of 150.89 cm, the number of leaves 41.22 sheets and a stem diameter of 12.86 mm. The seedlings that inoculated with potential microbes can be used for rehabilitation of old cacao trees.

scholarly journals Growth Response and Yield of Three Shallot Varieties on The Various Mixed of UREA + ZA in Ultisol Soil

2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Lily Marlina ◽  
Joko Purnomo ◽  
Hilda Susanti
Keyword(s):  
Plant Height ◽  
Interaction Effect ◽  
Growth Response ◽  
Growth And Yield ◽  
Affected Plant ◽  
Split Plot ◽  
Main Plot ◽  
Number Of Leaves ◽  
Four Levels ◽  
Plot Design

This study aimed to: (1) identify the interaction effect between varieties and mixed doses of urea + ZA on the growth and yield of shallots in Ultisol soil; (2) to identify the effect of each shallot varieties and the dosage mixture of urea + ZA which produce the highest yield in Ultisol soils. The research was conducted from April to June 2019 in Tungkaran Village, Martapura, with a split plot design. The main plot was shallot varieties (V) with three levels, namely Biru Lancor (v1), Bima Brebes (v2), and Super Philip (v3). Subplots were a mixture of urea + ZA (P) with four levels, namely 0 kg urea + 600 kg ZA (p1), 100 kg urea + ZA 400 kg (p2), 200 kg urea + 200 kg ZA (p3), and 300 urea kg + ZA 0 kg (p4). Each experiment was repeated three times. Therefore, there were 36 experimental units. Observation parameters included plant height, number of leaves per clump, number of bulbs per clump, fresh bulbs' weight per clump, and yield of stored dry bulbs. The results showed that the Bima Brebes variety (v2) had a significant effect on plant height, number of leaves, number of bulbs per clump, the weight of fresh bulbs per clump, and yield of stored dry bulbs. The mixture of 200 kg urea + 200 g ZA (p3) significantly affected plant height, number of leaves, number of tubers per clump, the weight of fresh tubers per clump, and yield of stored dry tubers.

Induksi Produksi Asiatikosida Pegagan (Centella asiatica [L] Urban) Menggunakan Stimulan Biologi Pada Kondisi Ternaungi

2019 ◽  
Vol 15 (3) ◽  
Author(s):  
Yuda Purwana Roswanjaya
Keyword(s):  
Vegetative Growth ◽  
Coffea Arabica ◽  
Centella Asiatica ◽  
Flemingia Macrophylla ◽  
Split Plot ◽  
Main Plot ◽  
Plot Design

This research studied the effect of Rhizobacteria on increasing the biomass and quality (asiaticoside content and production) of Indian Pennywort (Centella asiatica [L] Urban) under shaded condition. The experiment was arranged in split plot design. The main plot was shading condition (30% shading of Flemingia macrophylla and 30% shading of Coffee (Coffea arabica) trees) and the sub plot was Rhizobacteria inoculation (0 and 106 CFU/mL) with three replications. The result showed that vegetative growth and biomass of Indian Pennywort were not affected by Rhizobacteria inoculation and shading. However, Rhizobacteria inoculation increased asiaticoside content and production in Indian Pennywort.Studi ini dilakukan untuk mempelajari pengaruh Rhizobacteria sebagai stimulan biologi dalam meningkatkan biomasa dan kualitas (kadar dan produksi asiatikosida) pegagan (Centella asiatica [L] Urban) dalam kondisi ternaungi. Percobaan dirancang dalam Rancangan Petak Terbagi. Petak utama adalah naungan (Naungan Flemingia 30% dan naungan kopi 30%) dan anak petak adalah inokulasi Rhizobacteria (0 dan 106 CFU/mL) dengan tiga ulangan. Hasil percobaan menunjukkan bahwa pertumbuhan vegetatif dan biomasa pegagan tidak dipengaruhi oleh inokulasi Rhizobacteria dan naungan. Namun, inokulasi Rhizobacteria dapat meningkatkan kadar dan produksi asiatikosida pegagan.Keywords: Rhizobacteria, triterpenoid, cekaman naungan

scholarly journals Pengaruh Jenis Eksplan dan Asam Amino pada Inisiasi dan Proliferasi Kalus Embriogenik Phalaenopsis Var. ‘Raiza Agrihorti’

2021 ◽  
Vol 31 (1) ◽  
pp. 11
Author(s):  
Fitri Rachmawati ◽  
Dedeh Siti Badriah ◽  
Budi Marwoto
Keyword(s):  
Amino Acids ◽  
Embryogenic Callus ◽  
Block Design ◽  
Ornamental Plants ◽  
Basal Part ◽  
Multiplication Rate ◽  
Commercial Scale ◽  
Callus Initiation ◽  
Split Plot ◽  
Main Plot

<p><strong>(<em>The Effect of Explant Types and Amino Acids on Embryogenic Callus Initiation and Proliferation of Phalaenopsis Var. ‘Raiza Agrihorti’</em>)</strong></p><p>Penyiapan kalus embriogenik (KE) yang optimal memiliki peranan penting dalam menghasilkan benih bermutu Phalaenopsis skala komersial. Kendala utama yang dihadapi ialah inisiasi dan proliferasi KE yang masih rendah, serta akumulasi fenolik yang tinggi. Penelitian dilakukan di Laboratorium Kultur Jaringan Balithi dari Agustus 2019 hingga Juli 2020. Penelitian menggunakan Rancangan Acak Kelompok (RAK) pola split plot dan faktorial dengan lima ulangan. Percobaan-1: jenis eksplan (pucuk, pangkal, dan daun plantlet) sebagai petak utama dan perlakuan asam amino (tanpa asam amino, L-Proline, L-Glutamine, L-Cysteine, dan Casein-Hydrolisate) dengan konsentrasi 150 mg/l pada medium PC1 (1/2 MS + 1,0 mg/l TDZ + 0,5 mg/l BAP + 20 g/l sukrosa) sebagai anak petak. Percobaan-2: faktor-1 ialah jenis asam amino (L-Proline, L-Cysteine; L-Glutamine, dan Casein-Hydrolisate) dan faktor-2 ialah konsentrasi asam amino (0, 75, 150, 225, dan 300 mg/l). Hasil penelitian menunjukkan bahwa inisiasi KE Phalaenopsis var. ‘Raiza Agrihorti’ terbaik didapatkan dari pangkal plantlet dan 150 mg/l L-Glutamine dengan waktu inisiasi 18,3-24,0 hari, 80-100% pembentukan KE, dan ukuran KE 0,4-0,5 cm3. Proliferasi KE terbaik ditemukan pada L-Glutamine dengan konsentrasi 150 mg/l. Proliferasi KE mencapai 100% dengan penambahan berat segar sebesar 0,39 g, tingkat multiplikasi (MR) 4,55 kali dan pencokelatan 4,0%. Hasil penelitian ini berpotensi tinggi untuk diterapkan pada kultur starter Phalaenopsis hibrida lain.</p><p><strong>Keywords</strong></p><p>Phalaenopsis hibrid; Asam amino; Inisiasi; Kalus embriogenik; Proliferasi</p><p><strong>Abstract</strong></p><p>Setup of the optimum Phalaenopsis embryogenic callus (EC) is an important role in producing qualified-seedlings of Phalaenopsis in commercial scale. The main constraints that are still being faced are the low rate of culture proliferation and high phenolic accumulations. The research was carried out at the Tissue Culture Laboratory-Indonesian Ornamental Plants Research Institite, from August 2019 through July 2020. The split plot and factorial designs were arranged using a Randomized Completely Block Design (RCBD) with five replications. Experiment-1: explants type (shoot tip, basal part, and leaf of plantlet) was used as main plot and amino acids (amino acids free, L-Proline, L-Glutamine, L-Cysteine, and Casein-Hydrolisate) with 150 mg/l concentration on medium PC1 (1/2 MS + 1,0 mg/l TDZ + 0,5 mg/l BAP + 20 g/l sukrosa) as subplot. Experiment-2: the first factor was amino acids type (L-Proline, L-Cysteine; L-Glutamine, and Casein-Hydrolisate) and the second factor was amino acids concentration (0, 75, 150, 225, and 300 mg/l). Results of the studies revealed that the best EC initiation of Phalaenopsis var. ‘Raiza Agrihorti’ was produced by basal part of plantlet and PC1 medium containing 150 mg/l L-Glutamine with EC Initiation time was 18.3-24.0 days, 80-100% of EC formation and size of 0.4-0.5 cm3. The best proliferation of EC was found in L-Glutamine with 150 mg/l concentration. EC proliferation reached 100% with 4.55 EC multiplication rate, 0.39 g EC fresh weight added, and EC browning as low as 4.0%. The established method is high possibly applied for other Phalaenopsis hybrids.</p>

scholarly journals PERTUMBUHAN STUMP OKULASI MATA TIDUR KARET (Hevea brasiliensis Muell Arg.) SETELAH MENGALAMI PENYIMPANAN PADA MEDIA SABUT KELAPA

2017 ◽  
Vol 2 (2) ◽  
pp. 65
Author(s):  
Ridawati Marpaung
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Hevea Brasiliensis ◽  
Stem Diameter ◽  
Leaf Number ◽  
Coconut Fiber ◽  
Rubber Plant ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the growth eye stump of rubber after laying away in coconut fiber media. The design of this study was Completely Randomized Design with laying duration in coconut fiber medium (P) as a treatment which consisting of 7 treatment levels e.g. P0: 0 day; P1:5 days; P2:10 days, P3:15 days; P4:20 days, P5: 25 days, and P6: 30 days storage. The result of this research showed that the growth of eye stump rubber after laying away on the coconut fiber medium was significantly influence the percentage of several parameters e.g. :starting of stump sprout, sprout height during storage, plant height, stem diameter, leaf number and leaf area total for each treatment except for the parameter percentage of eye stump rubber which not active yet.  The freshness and the seedling viability of rubber plant originating from the eye stump rubber after laying away for 0-30 days in coconut fiber can be maintained. The highest growth was observed in the 20 to 30 days of laying away.Keywords: stump sleeping eye grafting, storage, coconut huskPenelitian ini bertujuan untuk mengetahui pertumbuhan stum okulasi mata tidur Karet (Hevea brasiliensis Muell Arg.) setelah mengalami  penyimpanan pada media sabut kelapa.  Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan perlakuan percobaan lama penyimpanan stum okulasi mata tidur dalam  media sabut kelapa (P) yang terdiri dari 7 taraf perlakuan yaitu penyimpanan:P0: 0 hari; P1:5 hari ; P2:10 hari, P3: 15 hari ; P4: 20 hari, P5: 25 hari, dan P6: penyimpanan 30 hari. Hasil penelitian menunjukkan bahwa pertumbuhan stum okulasi mata tidur karet (Hevea brasiliensis Muell Arg.) setelah mengalami penyimpanan pada media sabut kelapa berpengaruh nyata terhadap peubah persentase stum okulasi mulai bertunas, tinggi tunas selama penyimpanan, tinggi tanaman, diameter batang, jumlah daun dan luas daun total untuk setiap perlakuan kecuali pada peubah persentase stum okulasi mata tidur yang belum aktif tumbuh. Kesegaran dan daya tumbuh bibit tanaman karet yang berasal dari stum mata tidur setelah disimpan selama 0–30 hari dalam media sabut kelapa dapat dipertahankan. Pertumbuhan tertinggi untuk setiap peubah yang diamati terdapat pada lama penyimpanan 20 hingga 30 hari.Kata kunci : stump okulasi mata tidur, penyimpanan, sabut kelapa 

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