scholarly journals Optimization of Penicillium Lagena Medium Cultivication on Antifungal Pathogen of Phellinus Lamaoensis Using Surface Methode

Teknik ◽  
2018 ◽  
Vol 38 (2) ◽  
pp. 58
Author(s):  
Siti Nabilah ◽  
Rofiq Sunaryanto ◽  
Khaswar Syamsu
Keyword(s):  
Yeast Extract ◽  
Active Compound ◽  
Statistical Design ◽  
Medium Composition ◽  
Quadratic Model ◽  
Ageratum Conyzoides ◽  
Medium Components ◽  
Mineral Solution ◽  
Solution Verification ◽  
Rot Disease

Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233%  with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.

scholarly journals OPTIMASI PERMUKAAN RESPON MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense

2019 ◽  
Vol 6 (2) ◽  
pp. 164
Author(s):  
Rofiq Sunaryanto ◽  
Diana Nurani
Keyword(s):  
Central Composite Design ◽  
Yeast Extract ◽  
Active Compound ◽  
Carbon Sources ◽  
Pathogenic Fungi ◽  
Medium Composition ◽  
Quadratic Model ◽  
Active Compounds ◽  
Area Unit ◽  
Central Composite

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.

scholarly journals Increase of bacteriocin-like activity of Bacillus thuringiensis strain by improving nutriculture medium composition

2020 ◽  
Vol 50 (2) ◽  
pp. 47-56
Author(s):  
G. V. Kalmykova ◽  
A. F. Cheshkova ◽  
N. I. Akulova
Keyword(s):  
Antimicrobial Activity ◽  
Linear Regression ◽  
Yeast Extract ◽  
Nutrient Medium ◽  
Medium Composition ◽  
Second Order ◽  
Quadratic Model ◽  
Quantitative Composition ◽  
Medium Components ◽  
Multifactor Experiment

The development of the nutriculture medium composition and the optimal concentrations of its constituent components to increase the bacteriocin-like activity of the strain Bacillus thuringiensis ssp. Dakota are presented. The study was carried out using a multifactor experiment with further processing of statistical data in order to optimize the basic nutriculture medium and maximize the activity of the target product. Sources of nitrogen (peptone and yeast extract) and carbon (glycerin and glucose) were used as optimization factors. The control growth medium was medium "A", traditionally used for the cultivation of bacteria of the genus Bacillus. The degree of impact of the studied factors on the effective feature was determined using the model of multiple linear regression of the first order, whereby the optimal ratio of the components was calculated on the basis of the quadratic model. The ability of the Bacillus thuringiensis ssp. dakota strain to produce a bacteriocin-like substance (BLIS) was revealed. The dependence of BLIS synthesis on the cultivation medium was established: on the carbohydrate-free medium, the antimicrobial activity of BLIS was 1.5 times lower than that on the medium containing glucose and glycerin. To obtain maximum BLIS activity, the carbohydrate medium was optimized using a multifactor experiment performed by the method of orthogonal Latin rectangles. Mathematical models of linear regression of the first and second order were constructed depending on the concentration of nutrient medium components. The optimal concentration of the components was determined on the basis of a second-order regression model that takes into account the effects of the interaction of factors and the nonlinearity of the process. As a result of optimization of the nutrient medium, the quantitative composition of the components of the culture medium was determined: peptone – 9 g/l; yeast extract – 2.6; glycerol – 5.6 g/l, on which the antimicrobial activity of BLIS increased by 60% compared with the activity on the initial medium. It was shown that the synthesis of BLIS, unlike the synthesis of delta-endotoxin, is not regulated by catabolite repression of carbon.

Optimization of Medium Components for β-Glucosidase Production from Aspergillus Niger by Response Surface Methodology

2013 ◽  
Vol 419 ◽  
pp. 328-333 ◽  
Author(s):  
Chao Zhang ◽  
Rui Huang ◽  
Hui Tian ◽  
Ru Ming Zhao ◽  
Fa Shun Yu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Influence Factors ◽  
Mutual Effect ◽  
Tween 80 ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Malt Extract ◽  
Medium Components

β-Glucosidase is the key enzyme for the utilization of lignocellulose.But the commercial β-glucosidase can’t be produced. This paper focuses on the study of the β-glucosidase fermentation process.The fermentation medium components for β-glucosidase production from Aspergil lusniger was optimized by response surface methodology (RSM). Firstly, the three of the most important influence factors yeast extract, MnSO4•H2O and MgSO4•7H2O was obtained from Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration and mutual effect of three factors were predicted by RSM. The results showed that the best medium composition was Malt extract 18g/L, Yeast extract 3.22g/L, KH2PO4 3g/L, MnSO4•H2O 0.58mM, Tween-80 0.5mL/L and MgSO4•7H2O 0.23g/L. Under these fermentation conditions, the activity of β-glucosidase was up to 7.33IU/mL with increasing 23.2% than before.

scholarly journals Statistical Approach to Optimize Production of Biosurfactant by Achromobacter Xylos

2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Reddy Golamari Siva ◽  
Himavarshini Kadiyala ◽  
Prasanna Asapu Devi ◽  
Harini Singavarapu ◽  
Nikitha Narra Sai ◽  
...  
Keyword(s):  
Life Forms ◽  
Medium Composition ◽  
Fuel Oil ◽  
Quadratic Model ◽  
Test Results ◽  
Medium Components ◽  
Pull Out ◽  
Surfactant Production ◽  
Central Composite ◽  
The Ideal

This study aimed to optimize medium composition biosurfactant production of achromobacter xylos using response surface quadratic model . Lipoprotein and lipopeptides are used in many industries such as petroleum refining, pharmaceutical, mining, agriculture and bioprocess industries. The point of this assessment was to pull out and portray the biosurfactant passing on restriction of microorganisms from oil corrupted soil and considering their advancement energy at various temperatures and pH. The separation and growth study was directed in MSM medium using lamp fuel oil as sole carbon hotspot for bacterial turn of events. Confined strains were found to be Gram positive bacillus and in general Gram's positive minuscule life forms can convey lipopeptides type biosurfactants. The ideal conditions for achromobacteria xylos growth were discovered to be at pH seven (7) and temperature 30oC. Central composite design (CCD) was utilized to pick the following medium components (MgSO4, NaNO3, CaCl2, (NH4)2SO4, FeSO4, and KH2PO4). Central composite arrangement (CCD) of RSM was utilized to analyze the four parts at five stages, and biosurfactant fixation was evaluated as reaction. Backslide coefficients were directed by backslide examination, and the quadratic model condition was settled. R2 an impetus for bio-surfactant was endeavored to be 0.7527, showing that the quadratic model was basic with the exploratory outcomes. Confirmation of the numerical model was driven by playing out the assessment with the normal overhauled values, and bio-surfactant production was found to be 10.53 g/L. Underwriting of the normal quadratic model was 97.3% exact with the test results facilitated under the ideal conditions. CaCl2, (NH4)2SO4, FeSO4, and KH2PO4 were perceived as successful portions for bio-surfactant delivering 98% of achromobacter xylos microorganism.

scholarly journals Aplicación de diseños estadísticos secuenciales en la identificación de fuentes nutricionales para Azotobacter chroococcum AC1.

2011 ◽  
Vol 12 (2) ◽  
pp. 151 ◽  
Author(s):  
Andrés Eduardo Moreno G. ◽  
Daniel Fernando Rojas T. ◽  
Ruth Rebeca Bonilla B.
Keyword(s):  
Yeast Extract ◽  
Monosodium Glutamate ◽  
Statistical Design ◽  
Azotobacter Chroococcum ◽  
Fundamental Aspect ◽  
Plant Growth Promoting Bacteria ◽  
Experimental Strategy ◽  
Mineral Solution ◽  
Plant Growth Promoting ◽  
Statistical Designs

<p>La multiplicación masiva de bacterias promotoras de crecimiento vegetal es un aspecto fundamental para la producción de bioinoculantes. Con el objetivo de evaluar una estrategia experimental que permitiera identificar factores nutricionales con influencia sobre la multiplicación de Azotobacter chroococcum AC1, se realizó la aplicación secuencial de diseños estadísticos (Placket-Burman, diseño factorial 27-3, máxima respuesta ascendente y análisis de superficie de respuesta). Se evaluaron once fuentes nutricionales: glucosa, sacarosa, glicerol, almidón, glutamato monosódico, urea, levadura comercial entera, extracto de levadura, MgSO4·7H2O, K2HPO4·3H2O y solución de microelementos. Los resultados evidenciaron que la aplicación en secuencia de diseños estadísticos demostró ser una estrategia confiable permitiendo una producción de células viables de 9x109 ufc/mL luego de 24 horas del proceso de multiplicación, empleando una combinación óptima estimada basada en extracto de levadura, glutamato monosódico, glucosa, K2HPO4·3H2O, MgSO4·7H2O y solución de micronutrientes.</p><p> </p><p><strong>Sequential statistical design application in identification of Azotobacter chroococcum AC1 nutritional sources.</strong></p><p>The mass multiplication of plant growth promoting bacteria is a fundamental aspect in the production of bioinoculants. In order to evaluate an experimental strategy that would identify nutritional factors that influence the growth of Azotobacter chroococcum AC1 strain, the sequential application of statistical designs (Placket-Burman design, 27-3 factorial design, steepest ascent method, and response surface analysis) was performed. Eleven nutritional sources: glucose, sucrose, glycerol, starch, monosodium glutamate, urea, commercial yeast, yeast extract, MgSO4·7H2O, K2HPO4·3H2O, and mineral solution were evaluated. Sequential statistical design application proved to be a reliable experimental strategy, allowing 9x109 cfu/ mL production from an optimal ration between yeast extract, monosodium glutamate, glucose, K2HPO4·3H2O, MgSO4·7H2O, and mineral solution.</p>

Evaluation of nitrogenous media components by Plackett–Burman statistical design for β-d-fructofuranosidase production by Saccharomyces sp. strain GVT263

2009 ◽  
Vol 55 (4) ◽  
pp. 405-409 ◽  
Author(s):  
M. Venkateshwar ◽  
K. Chaitanya ◽  
M. D. Altaf ◽  
B. Hameeda ◽  
M. Ghopal Reddy
Keyword(s):  
Experimental Design ◽  
Yeast Extract ◽  
Ammonium Acetate ◽  
Statistical Design ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Statistical Experimental Design ◽  
Meat Extract ◽  
Medium Components ◽  
Sucrose Fermentation

β-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using a high enzyme-producing yeast strain. Plackett–Burman statistical experimental design was applied to evaluate the fermentation medium components. The effects of 10 nitrogen sources were studied in a 16-run experimental design. Beef extract, yeast extract, N-Z-amine, tryptone, meat extract, and ammonium acetate were found to have significant effects on enzyme production. Among these, yeast extract, N-Z-amine, and ammonium acetate were the most significant. A maximum FFase activity of 299.4 U/mL was obtained after a 24 h fermentation period.

scholarly journals Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase fromAspergillus nigerATCC 20611

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Mojdeh Dinarvand ◽  
Malahat Rezaee ◽  
Malihe Masomian ◽  
Seyed Davoud Jazayeri ◽  
Mohsen Zareian ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Scale Up ◽  
Medium Composition ◽  
Quadratic Model ◽  
Coefficient Of Determination ◽  
Main Reaction ◽  
Effective Response ◽  
Triton X

The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes fromAspergillus nigerATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO3, Zn+2, and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R2) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO3, 1.5 mM (v/v) Zn+2, and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry.

scholarly journals Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Deepmoni Deka ◽  
P. Bhargavi ◽  
Ashish Sharma ◽  
Dinesh Goyal ◽  
M. Jawed ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Yeast Extract ◽  
Cellulase Activity ◽  
Fold Increase ◽  
Medium Composition ◽  
Medium Components ◽  
Burman Design ◽  
Full Factorial ◽  
Good Agreement

The cellulase activity of Bacillus subtilis AS3 was enhanced by optimizing the medium composition by statistical methods. The enzyme activity with unoptimised medium with carboxymethylcellulose (CMC) was 0.07 U/mL and that was significantly enhanced by CMC, peptone, and yeast extract using Placket-Burman design. The combined effects of these nutrients on cellulase activity were studied using 22 full factorial central composite design. The optimal levels of medium components determined were CMC (1.8%), peptone (0.8%), and yeast extract (0.479%). The maximum enzyme activity predicted by the model was 0.49 U/mL which was in good agreement with the experimental value 0.43 U/mL showing 6-fold increase as compared to unoptimised medium. The enzyme showed multisubstrate specificity, showing significantly higher activity with lichenan and β-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse. The optimised medium with lichenan or β-glucan showed 2.5- or 2.8-fold higher activity, respectively, at same concentration as of CMC.

scholarly journals Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Atef M. Ibrahim ◽  
Ragaa A. Hamouda ◽  
Noura El-Ahmady El-Naggar ◽  
Fatma M. Al-Shakankery
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Yeast Extract ◽  
Statistical Design ◽  
Life Time ◽  
Maximum Activity ◽  
Bioprocess Development ◽  
Ammonium Sulfate Precipitation ◽  
Face Centered ◽  
Central Composite

AbstractEndoglucanase producing bacteria were isolated from Egyptian soils and the most active bacterial strain was identified as Bacillus subtilis strain Fatma/1. Plackett–Burman statistical design was carried out to assess the effect of seven process variables on endoglucanase production. Carboxymethyl cellulose (CMC), yeast extract and peptone were the most significant variables that enhanced the endoglucanase production and thus were selected for further optimization using face-centered central composite design. The highest yield of endoglucanase (32.37 U/mL) was obtained in run no. 9, using 18 g/L CMC, 8 g/L peptone, 7 g/L yeast extract and 0.1 g/L FeSO4.7H2O. The optimized medium showed about eightfold increase in endoglucanase production compared to the unoptimized medium. The produced crude enzyme was further purified by ammonium sulfate precipitation, then DEAE-Sepharose CL6B column. The purified enzyme was shown to have a molecular weight of 37 kDa. The enzyme showed maximum activity at pH 8.0, temperature of 50 °C, incubation time of 60 min. The half-life time (T1/2) was 139.53 min at 50 °C, while being 82.67 min at 60 °C. Endoglucanase at concentration of 12 U/mL effectively removed 84.61% of biofilm matrix of Pseudomonas aeruginosa with marked reduction in carbohydrate content of the biofilm from 63.4 to 7.9 μg.

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

2014 ◽  
Vol 522-524 ◽  
pp. 295-298
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Biological Control ◽  
Yeast Extract ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Peanut Hulls ◽  
Two Factors ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design ◽  
Biocontrol Bacterium

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD600 observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

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