Salt overly sensitive 1 (SOS1) gene expression can be regulated via Azospirillum brasilense Sp7 in wheat seedlings under saline condition

Salinity stress reduces plant growth via failure of physiological processes mainly due to the abundance of Na+ ion. Salt overly sensitive (SOS) signaling pathway is considered as an important component of Na+/K+ homeostasis system in plants, especially under saline condition. Moreover, it is reported that wheat-Azospirillum associated has resulted in an enhanced salinity tolerance. To evaluate involvement of Azospirillum species in regulation of SOS signaling pathway, inoculated and none-inoculated wheat seedlings with Azospirillum brasilense Sp7 were grown for five days. Then uniform seedlings were transferred into saline hydroponic media with and without 200 mM NaCl. The relative expression of TaSOS1 of root, sheath, and blade as well as Na+/K+ ratio was measured after 6, 24 and 48 hours since inoculated and non-inoculated seedling were transferred to NaCl media. Simultaneously Ca, Fe, proline content, root and shoot dry mass and soluble sugars were measured at 72 hour after application of NaCl. Result showed that salinity increased TaSOS1 gene expression, Na+, prolin and Na+/K+ ratio but Ca and Fe were decreased in root and shoot of wheat seedlings. Although A. brasilense Sp7 could improve salinity tolerance in wheat via reduction of Na uptake and upregulation of TaSOS1 expression, but do not have any effect in sodium distribution within plant parts. Therefore, salinity could increase TaSOS1 expression in the root, sheath and blade and A. brasilense Sp7 also could reduce the adverse effect of salinity via addition of over expression of TaSOS1.

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TASOS1 AND TATM20 GENES EXPRESSION AND NUTRIENT UPTAKE IN WHEAT SEEDLINGS MAY BE ALTERED VIA EXCESS CADMIUM EXPOSURE AND INOCULATION WITH AZOSPIRILLUM BRASILENSE SP7 UNDER SALINE CONDITION

Applied Ecology and Environmental Research ◽

10.15666/aeer/1602_17971817 ◽

2018 ◽

Vol 16 (2) ◽

pp. 1797-1817 ◽

Cited By ~ 2

Author(s):

H. R. GHASSEMI

Keyword(s):

Nutrient Uptake ◽

Azospirillum Brasilense ◽

Cadmium Exposure ◽

Saline Condition ◽

Wheat Seedlings ◽

Genes Expression ◽

Azospirillum Brasilense Sp7

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PI3K signaling Pathway gene expression analysis in cumulus cells by Real-Time PCR

10.26226/morressier.573c1511d462b80296c982f0 ◽

2016 ◽

Author(s):

Paolo Giovanni Artini

Keyword(s):

Gene Expression ◽

Real Time ◽

Signaling Pathway ◽

Real Time Pcr ◽

Gene Expression Analysis ◽

Cumulus Cells ◽

Pi3k Signaling ◽

Pathway Gene ◽

Pi3k Signaling Pathway ◽

Signaling Pathway Gene

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Mot3, a Zn Finger Transcription Factor That Modulates Gene Expression and Attenuates Mating Pheromone Signaling in Saccharomyces cerevisiae

Genetics ◽

10.1093/genetics/149.2.879 ◽

1998 ◽

Vol 149 (2) ◽

pp. 879-892 ◽

Cited By ~ 1

Author(s):

Anatoly V Grishin ◽

Michael Rothenberg ◽

Maureen A Downs ◽

Kendall J Blumer

Keyword(s):

Gene Expression ◽

Saccharomyces Cerevisiae ◽

Signaling Pathway ◽

G Protein ◽

Binding Sites ◽

Dependent Manner ◽

Pheromone Response ◽

Mating Pheromone ◽

Pheromone Signaling ◽

Yeast Genes

Abstract In the yeast Saccharomyces cerevisiae, mating pheromone response is initiated by activation of a G protein- and mitogen-activated protein (MAP) kinase-dependent signaling pathway and attenuated by several mechanisms that promote adaptation or desensitization. To identify genes whose products negatively regulate pheromone signaling, we screened for mutations that suppress the hyperadaptive phenotype of wild-type cells overexpressing signaling-defective G protein β subunits. This identified recessive mutations in MOT3, which encodes a nuclear protein with two Cys2-His2 Zn fingers. MOT3 was found to be a dosage-dependent inhibitor of pheromone response and pheromone-induced gene expression and to require an intact signaling pathway to exert its effects. Several results suggested that Mot3 attenuates expression of pheromone-responsive genes by mechanisms distinct from those used by the negative transcriptional regulators Cdc36, Cdc39, and Mot2. First, a Mot3-lexA fusion functions as a transcriptional activator. Second, Mot3 is a dose-dependent activator of several genes unrelated to pheromone response, including CYC1, SUC2, and LEU2. Third, insertion of consensus Mot3 binding sites (C/A/T)AGG(T/C)A activates a promoter in a MOT3-dependent manner. These findings, and the fact that consensus binding sites are found in the 5′ flanking regions of many yeast genes, suggest that Mot3 is a globally acting transcriptional regulator. We hypothesize that Mot3 regulates expression of factors that attenuate signaling by the pheromone response pathway.

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Microarray analysis identification of key pathways and interaction network of differential gene expressions during osteogenic differentiation

Human Genomics ◽

10.1186/s40246-020-00293-1 ◽

2020 ◽

Vol 14 (1) ◽

Author(s):

Fatemeh Khodabandehloo ◽

Sara Taleahmad ◽

Reza Aflatoonian ◽

Farzad Rajaei ◽

Zahra Zandieh ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Signaling Pathway ◽

Expression Profiles ◽

Wnt Signaling Pathway ◽

Hub Genes ◽

Gene Expressions ◽

Cell Based Therapy ◽

Wnt Pathways ◽

Key Pathways

Abstract Background Adult bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stem cells that can differentiate into three lineages. They are suitable sources for cell-based therapy and regenerative medicine applications. This study aims to evaluate the hub genes and key pathways of differentially expressed genes (DEGs) related to osteogenesis by bioinformatics analysis in three different days. The DEGs were derived from the three different days compared with day 0. Results Gene expression profiles of GSE37558 were obtained from the Gene Expression Omnibus (GEO) database. A total of 4076 DEGs were acquired on days 8, 12, and 25. Gene ontology (GO) enrichment analysis showed that the non-canonical Wnt signaling pathway and lipopolysaccharide (LPS)-mediated signaling pathway were commonly upregulated DEGs for all 3 days. KEGG pathway analysis indicated that the PI3K-Akt and focal adhesion were also commonly upregulated DEGs for all 3 days. Ten hub genes were identified by CytoHubba on days 8, 12, and 25. Then, we focused on the association of these hub genes with the Wnt pathways that had been enriched from the protein-protein interaction (PPI) by the Cytoscape plugin MCODE. Conclusions These findings suggested further insights into the roles of the PI3K/AKT and Wnt pathways and their association with osteogenesis. In addition, the stem cell microenvironment via growth factors, extracellular matrix (ECM), IGF1, IGF2, LPS, and Wnt most likely affect osteogenesis by PI3K/AKT.

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Transcriptional Profiling and Biological Pathway(s) Analysis of Type 2 Diabetes Mellitus in a Pakistani Population

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17165866 ◽

2020 ◽

Vol 17 (16) ◽

pp. 5866

Author(s):

Zarish Noreen ◽

Christopher A. Loffredo ◽

Attya Bhatti ◽

Jyothirmai J. Simhadri ◽

Gail Nunlee-Bland ◽

...

Keyword(s):

Gene Expression ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Mitochondrial Dysfunction ◽

Signaling Pathway ◽

Health Concern ◽

Receptor Signaling ◽

Small Pilot Study

The epidemic of type 2 diabetes mellitus (T2DM) is an important global health concern. Our earlier epidemiological investigation in Pakistan prompted us to conduct a molecular investigation to decipher the differential genetic pathways of this health condition in relation to non-diabetic controls. Our microarray studies of global gene expression were conducted on the Affymetrix platform using Human Genome U133 Plus 2.0 Array along with Ingenuity Pathway Analysis (IPA) to associate the affected genes with their canonical pathways. High-throughput qRT-PCR TaqMan Low Density Array (TLDA) was performed to validate the selected differentially expressed genes of our interest, viz., ARNT, LEPR, MYC, RRAD, CYP2D6, TP53, APOC1, APOC2, CYP1B1, SLC2A13, and SLC33A1 using a small population validation sample (n = 15 cases and their corresponding matched controls). Overall, our small pilot study revealed a discrete gene expression profile in cases compared to controls. The disease pathways included: Insulin Receptor Signaling, Type II Diabetes Mellitus Signaling, Apoptosis Signaling, Aryl Hydrocarbon Receptor Signaling, p53 Signaling, Mitochondrial Dysfunction, Chronic Myeloid Leukemia Signaling, Parkinson’s Signaling, Molecular Mechanism of Cancer, and Cell Cycle G1/S Checkpoint Regulation, GABA Receptor Signaling, Neuroinflammation Signaling Pathway, Dopamine Receptor Signaling, Sirtuin Signaling Pathway, Oxidative Phosphorylation, LXR/RXR Activation, and Mitochondrial Dysfunction, strongly consistent with the evidence from epidemiological studies. These gene fingerprints could lead to the development of biomarkers for the identification of subgroups at high risk for future disease well ahead of time, before the actual disease becomes visible.

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Effects of zearalenone-induced oxidative stress and Keap1–Nrf2 signaling pathway-related gene expression in the ileum and mesenteric lymph nodes of post-weaning gilts

Toxicology ◽

10.1016/j.tox.2019.152337 ◽

2020 ◽

Vol 429 ◽

pp. 152337 ◽

Cited By ~ 4

Author(s):

Qun Cheng ◽

Shuzhen Jiang ◽

Libo Huang ◽

Yuxi Wang ◽

Weiren Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Lymph Nodes ◽

Signaling Pathway ◽

Related Gene ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Nrf2 Signaling Pathway

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Molybdenum incorporation in denitrifying Azospirillum brasilense Sp7

Canadian Journal of Microbiology ◽

10.1139/m92-171 ◽

1992 ◽

Vol 38 (10) ◽

pp. 1042-1047 ◽

Cited By ~ 2

Author(s):

Christian Chauret ◽

Wilfredo L. Barraquio ◽

Roger Knowles

Keyword(s):

Nitrate Reductase ◽

Key Words ◽

Nitrate Reduction ◽

Gel Electrophoresis ◽

Azospirillum Brasilense ◽

Paracoccus Denitrificans ◽

Free Medium ◽

Azospirillum Brasilense Sp7

Nondenaturating disc gel electrophoresis revealed that 99Mo was incorporated into the nitrate reductase of Azospirillum brasilense grown in the absence but not in the presence of tungstate. Under denitrifying conditions, A. brasilense grown in tungsten-free medium steadily accumulated 99Mo for 12 h. In contrast, Paracoccus denitrificans grown under the same conditions ceased uptake after 1 h. However, both bacteria were incapable of accumulating significant amounts of 99Mo in media containing 10 mM tungstate, even though nitrate was reduced by A. brasilense. Aerobically grown A. brasilense cells transported 99Mo more efficiently than anaerobically grown cells. Key words: Azospirillum brasilense, tungsten, molybdenum incorporation, nitrate reduction.

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Fibroblast Growth Factors Regulate Prolactin Transcription via an Atypical Rac-Dependent Signaling Pathway

Molecular Endocrinology ◽

10.1210/me.2003-0167 ◽

2003 ◽

Vol 17 (10) ◽

pp. 1921-1930 ◽

Cited By ~ 12

Author(s):

Twila A. Jackson ◽

David M. Koterwas ◽

Melissa A. Morgan ◽

Andrew P. Bradford

Keyword(s):

Gene Expression ◽

Growth Factors ◽

Signaling Pathway ◽

Promoter Activity ◽

Fibroblast Growth Factors ◽

Dominant Negative ◽

Pituitary Cells ◽

Dependent Manner ◽

Fibroblast Growth ◽

Prl Gene

Abstract Fibroblast growth factors (FGFs) play a critical role in pituitary development and in pituitary tumor formation and progression. We have previously characterized FGF signal transduction and regulation of the tissue-specific rat prolactin (rPRL) promoter in GH4 pituitary cells. FGF induction of rPRL transcription is independent of Ras, but mediated by a protein kinase C-δ (PKCδ)-dependent activation of MAPK (ERK). Here we demonstrate a functional role for the Rho family monomeric G protein, Rac1, in FGF regulation of PRL gene expression via an atypical signaling pathway. Expression of dominant negative Rac, but not RhoA or Cdc42, selectively inhibited FGF-induced rPRL promoter activity. Moreover, expression of dominant negative Rac also attenuated FGF-2 and FGF-4 stimulation of MAPK (ERK). However, in contrast to other Rac-dependent signaling pathways, FGF activation of rPRL promoter activity was independent of the c-Jun N-terminal kinase (JNK) and phosphoinositide 3-kinase/Akt cascades. FGFs failed to activate JNK1 or JNK2, and expression of dominant negative JNK or Akt constructs did not block FGF-induced PRL transcription. Consistent with the role of PKCδ in FGF regulation of PRL gene expression, activation of the rPRL promoter was blocked by an inhibitor of phospholipase Cγ (PLCγ) activity. FGF treatment also induced rapid tyrosine phosphorylation of PLCγ in a Rac-dependent manner. These results suggest that FGF-2 and FGF-4 activate PRL gene expression via a novel Rac1, PLCγ, PKCδ, and ERK cascade, independent of phosphoinositol-3-kinase and JNK.

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