scholarly journals Selected physicochemical and solubilization properties of pharmacopeal solutions of dry green tea leaf extract (Ext. Camellia sinensis L. folium aqu. siccum)

2018 ◽  
Vol 64 (2) ◽  
pp. 68-76
Author(s):  
Zbigniew Marczyński ◽  
Beata Skibska ◽  
Sławomira Nowak ◽  
Izabela Rychlińska ◽  
Jerzy Jambor ◽  
...  
Keyword(s):  
Green Tea ◽  
High Performance ◽  
Thermal Exposure ◽  
Green Tea Extract ◽  
Oral Dosage ◽  
Solid Oral Dosage Form ◽  
Preventive Activity ◽  
Tea Extract ◽  
Tea Leaf ◽  
Risk Of Cancer

Summary Introduction: Green tea offers not only pleasant, delicate flavor, but also provides health benefits. The extract contains, among others, polyphenols responsible for antioxidant and anti-inflammatory properties. They reduce the risk of cancer and their presence exerts preventive activity against cardiovascular diseases. Objective: Analysis of selected physicochemical and solubilizing properties of pharmacopoeial-true solutions of dry green tea extract. Methods: The caffeine content was determined in the extract and in dry residue after solubilization by high performance liquid chromatography. The process of micellar solubilization of cholesterol granules and ketoprofen was carried out in model solutions of green tea extract. Results: The obtained results indicate that the prepared ‘ex tempore’ leaf green tea infusion subjected to short thermal exposure will be characterized by significant solubilization abilities. Conclusions: The outcomes of the research pointed to the possibility of developing a solid oral dosage form with titrated dry green tea extract of expected pharmacotherapeutic profile.

scholarly journals Development and Validation of a HPLC Method of Quantification of Caffeine and EGCG in Green Tea (Camellia sinensis L.) Extract

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Tra Trong Bien ◽  
Bui Thi Lan Phuong
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
High Performance ◽  
Protective Role ◽  
Hplc Method ◽  
Green Tea Extract ◽  
Ultrahigh Pressure ◽  
Bioactive Constituents ◽  
Tea Catechins ◽  
Tea Extract

This study investigates a simple high performance liquid chromatography (HPLC) in an isocratic eluent manner for quantification of caffeine and EGCG in green tea extract. The study separation system contained a C18-RP column (250 × 4.6 mm, 5 µm), a mobile phase of methanol/phosphoric acid/water (20/0.1/79.9, v/v) and a DAD detector at 280 nm. This system proves convenient for rapid routine analysis of caffeine and EGCG in green tea extract with good repeatability and accuracy. Keywords HPLC, caffeine, EGCG, green tea, extract. References [1] A. Chowdhury, J. Sarkar, T. Chakraborti, P.K. Pramanik, S. Chakraborti, Protective role of epigallocatechin-3-gallate in health and disease: A perspective, Biomedicine & Pharmacotherapy 78 (2016) 50-59. https://doi.org/10.1016/j.biopha.2015.12.013.[2] Y. Hara, Tea catechins and their applications as supplements and pharmaceutics, Pharmacological Research 64(2) (2011) 100-104. https://doi.org/10.1016/j.phrs.2011.03.018.[3] X. Jun, S. Zhao, B. Lu, R. Zhang, Y. Li, D. Shen, G. Zhou, Separation of major catechins from green tea by ultrahigh pressure extraction, International Journal of Pharmaceutics 386(1-2) (2010) 229-231. https://doi.org/10.1016/j.ijpharm.2009.10.035.[4] Z. Bedrood, M. Rameshrad, H. Hosseinzadeh, Toxicological effects of Camellia sinensis (green tea): A review, Phytotherapy Research 32(7) (2018) 1163-1180. https://doi.org/10.1002/ptr.6063.[5] J. Michael Glade, Caffeine-Not just a stimulant, Nutrition 26(10) (2010) 932-938. https://doi.org/10.1016/j.nut.2010.08.004.[6] MediGene AG. Press Release., MediGene AG Obtains Approval of Polyphenon®E Ointment in the USA, 2006.[7] Q.V. Vuong, V. Nguyen, J.B. Golding, P.D. Roach, The content of bioactive constituents as a quality index for Vietnamese teas, International Food Research Journal 18(1) (2011) 329-336.[8] Tran Trong Bien, Method for caffeine removal and catechins enrichment from green tea leaf, Master thesis, Hanoi University of Pharmacy, 2019 (In Vietnamese).[9] J. Joseph Dalluge, C. Bryant Nelson, Determination of tea catechins, Journal of Chromatography A 881 (2000) 411–424. https://doi.org/10.1016/S0021-9673(00)00062-5.[10] T. Shiono, et al., Selective decaffeination of tea extracts by montmorillonite, Journal of Food Engineering 200 (2017) 13-21. https://doi.org/10.1016/j.jfoodeng.2016.12.015.    

scholarly journals Studi In-Vivo Ekstrak Daun Teh Hijau (Camellia Sinensis) sebagai Alternatif anti Bakteri Eschericia Coli pada Ayam Broiler

2019 ◽  
Vol 37 (2) ◽  
pp. 172
Author(s):  
Bambang Sutrisno ◽  
R. Wasito ◽  
Kurniasih Kurniasih ◽  
Sitarina Widyarini ◽  
Yuli Purwandari Kristianingrum ◽  
...  
Keyword(s):  
Green Tea ◽  
Broiler Chickens ◽  
Leaf Extract ◽  
Histopathological Examination ◽  
Green Tea Extract ◽  
E Coli ◽  
Group B ◽  
Tea Extract ◽  
Tea Leaf

The prevalence of colibasillosis  in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml  water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5  Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied  with Mg SO4 saturated solution intravenously injection and necropsied  for gross and histpathological examination. Analysis of blood tests results were used one way of anova  (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P <0.05), in broilers infected with E. coli and given green tea extracts had lower amounts of hetrophils and fibrinogen levels. While blood TPP levels were not significantly different (P> 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens.

scholarly journals Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

2021 ◽  
Vol 23 (1) ◽  
pp. 132-137
Author(s):  
O. Yu. Maslov ◽  
S. V. Kolisnyk ◽  
O. V. Hrechana ◽  
A. H. Serbin
Keyword(s):  
Dietary Supplements ◽  
Green Tea ◽  
Leaf Extract ◽  
Green Tea Extract ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Tea Extract ◽  
Tea Leaf ◽  
Active Substances

Recently, there has been a high demand from the population for dietary supplements that have antioxidant activity. First of all, these are dietary supplements with green tea leaf extract, because green tea contains catechins in a fairly high amount. Nowadays, according to the current legislation of Ukraine the qualitative composition and quantitative content of biologically active substances in dietary supplements are not established, which to a certain extent affects the quality and safety of these products. The aim of the study was to determine the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Materials and methods. Three dietary supplements from different manufacturers “Green Tea Extract”, “Green Tea Extract”, “Green Tea” were chosen for the study. Qualitative analysis was performed by thin layer chromatography, for quantification spectrophotometry in the UV-region was used. Results. The presence of catechins was found in the studied dietary supplements, caffeine was found only in trace amounts. The total content of catechins is 146.80 ± 1.36 mg, 79.00 ± 0.88 mg, 28.00 ± 0.75 mg, and in terms of green tea extract, which is stated in dietary supplements for dietary supplements “Green Tea Extract”, “Green Tea Extract”, “Green Tea”, respectively. Conclusions. Qualitative and quantitative analysis of some groups of BAS of three dietary supplements with green tea leaf extract was performed. It was found that dietary supplements “Green Tea Extract” and “Green Tea” meet the requirements of the US Pharmacopoeia 38 in terms of catechins. Based on the study, it can be concluded that the problem of compliance with dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

Green Tea Extract Exposure Pattern Differenzially Affects Acetaminophen-Induced Hepatotoxicity

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
A Ali ◽  
X Yang ◽  
Q Shi ◽  
J Greenhaw ◽  
WF Salminen
Keyword(s):  
Green Tea ◽  
Green Tea Extract ◽  
Exposure Pattern ◽  
Tea Extract

Antioxidative Activity of Laver Snack Prepared with Addition of Green Tea Extract

2017 ◽  
Vol 23 (4) ◽  
pp. 35-41
Author(s):  
Jeong Hee Park ◽  
Hang Yeon Jeong ◽  
Jeong Yong Cho ◽  
Jae Hak Moon
Keyword(s):  
Green Tea ◽  
Antioxidative Activity ◽  
Green Tea Extract ◽  
Tea Extract

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

2020 ◽  
Vol 21 (1) ◽  
pp. 31-35
Author(s):  
Basma El-Desoky ◽  
Shaimaa El-Sayed ◽  
El-Said El-Said
Keyword(s):  
Gene Expression ◽  
Single Dose ◽  
Green Tea ◽  
Serum Testosterone ◽  
Green Tea Extract ◽  
Male Rats ◽  
Controlled Study ◽  
Control Group ◽  
Testicular Damage ◽  
Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

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