scholarly journals Effect of Fe3O4 and TiO2 Nanoparticles on Catalase Activity and β-Carotene Content at Pigmented Yeast Strain Rhodotorula gracilis

2017 ◽  
Vol 21 (1) ◽  
pp. 35-40
Author(s):  
Agafia Usatîi ◽  
Alina Beșliu ◽  
Nadejda Efremova
Keyword(s):  
Catalase Activity ◽  
Yeast Strain ◽  
Carotene Content ◽  
Wide Range ◽  
Rhodotorula Gracilis ◽  
Potential Applications ◽  
First Time ◽  
Antioxidant Enzyme Catalase ◽  
Β Carotene

AbstractThe properties of nanoparticles have been used in a wide range of potential applications in food industry, medicine, microbial biotechnology, cosmetics, environmental production. Research results of evaluation of the effect of Fe3O4and TiO2nanoparticles applicated in large concentration limits on some parameters of pigmented yeast strainRhodotorula gracilisCNMN-Y-30 are presented in this paper. It was established that nanoparticles selected for study caused toxic effects on β-caroten accumulation and activity of antioxidant enzyme catalase depending on concentration and nanostructure. The strong correlation between concentration of nanoparticles and evaluated components has been revealed at studied yeast strain. Coefficients of correlation varied in limits R2= 0.623... 0.951. For the first time, the determination of β-carotene accumulation and catalase activity rate as functional tests for estimation of toxicity of nanoparticles for pigmented yeast strainRhodotorula graciliswas effectuated. Thus, it was established that adaptive response of yeast strain to the presence of Fe3O4and TiO2nanoparticles has been manifested by modification of the processes of β-carotene biosythesis and catalase activity.

Development of a method to determine carotenoid composition of fresh forages

2008 ◽  
Vol 88 (6) ◽  
pp. 1057-1064 ◽  
Author(s):  
N. Cardinault ◽  
B. Lyan ◽  
M. Doreau ◽  
B. Chauveau ◽  
E. Rock ◽  
...  
Keyword(s):  
Key Words ◽  
Gradient System ◽  
Mountain Meadow ◽  
Coefficients Of Variation ◽  
Carotenoid Composition ◽  
Ruminant Diets ◽  
In Series ◽  
First Time ◽  
Β Carotene

Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on β-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C18 columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5–7.5 %) and inter-day (1.2–3.5 %) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants. Key words: Xanthophylls, carotenoids, fresh forage, HPLC

COTTONWEED MARSH (MARSH). CHEMICAL COMPOSITION OF ETHANOL EXTRACT

2020 ◽  
Vol 5 (4) ◽  
pp. 29-32
Author(s):  
V. Platonov ◽  
V. Dunaev ◽  
G. Suhih ◽  
M. Shatskiy ◽  
M. Volochaeva
Keyword(s):  
Chemical Composition ◽  
Mass Spectra ◽  
Ethanol Extract ◽  
Structural Group ◽  
Quantitative Content ◽  
Wide Range ◽  
Structural Formulas ◽  
First Time ◽  
Sulfur Containing

For the first time, a detailed study of the chemical composition of ethanol extract of dried marsh (swamp), a product obtained after preliminary exhaustive last ex-traction with n-hexane, toluene, chloroform and acetone was performed. Chromatography-mass spectrometry made it possible to identify 119 individual compounds in this extract with the determination of their quantitative content, mass spectra and structural formulas, and the calculation of the structural-group composition was performed. A characteristic feature of the extract is the presence of significant amounts of nitrogen and sulfur-containing compounds (20.04), esters (21.47), sterols (18.54), carboxylic acids (8.15) and hydrocarbons (7.15) (wt. % of the extract). In addition, the extract contains phenols, ketones, aldehydes, alcohols and glycosides: 5.01; 4.34; 4.88; 3.16 and 3.12 (wt. % of the extract), respectively. Dominance in the extract of nitrogen-sulfur-containing and compounds; environment of sterols-betulin and its derivatives, esters, as well as the presence of different structure of phenols, glycosides, aldehydes and alcohols makes it possible to assume a wide range of pharmacological activity of drugs based on ethanol extract of dried marsh (marsh).

scholarly journals Analisis Senyawa β-Karoten pada Buah Pepaya (Carica papaya L.) Asal Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara

2021 ◽  
Vol 4 (2) ◽  
pp. 1-7
Author(s):  
Irman Idrus ◽  
Sabda Wahab ◽  
Andi Fitrah Nugraha ◽  
Syaiful Bachri
Keyword(s):  
Quantitative Analysis ◽  
The Body ◽  
Test Results ◽  
Carotene Content ◽  
Test Parameter ◽  
Qualitative Test ◽  
Uv Visible ◽  
Planting Method ◽  
Β Carotene

South Konawe District at Southeast Sulawesi Province is a producer of agriculture such as Papaya fruit ranging from 1168 quintals each year. Papaya fruit contains β-carotene which plays an important role in the formation of vitamin A in the body. This research was conducted to determine the β-carotene content of papaya fruit by using variables planting method, fruitage, and fruit condition. The mashed fruit was extracted using n-hexane: acetone: ethanol (2: 1: 1) v/v, then separated from polar and non-polar solutions. Qualitative analysis was carried out using the Carr-Price method and quantitative analysis or determination of β-carotene levels using the UV-Visible spectrophotometric method λ = 452.0 nm. The qualitative test results showed that the papaya fruit in the papaya fruit obtained by cultivation and growing wild with each variable half-ripe, ripe, and not fresh papaya identified the presence of β-carotene. The results of quantitative analysis of β-carotene levels in cultivated papaya fruit were 1.76 μL, half-ripe 1.32 μL, and cultivated young papaya 1.22 μL. In wild ripe papaya 1.75 μL, wild half-ripe papaya 1.3 μL and wild young papaya 1.21 μL. There is an effect of each test parameter starting from the parameters of planting method, fruitage, and fruit condition on β-carotene content in papaya fruit.

scholarly journals A Rapid and Accurate Method for Estimating Tomato Lycopene Content by Measuring Chromaticity Values of Fruit Purée

2004 ◽  
Vol 129 (5) ◽  
pp. 717-723 ◽  
Author(s):  
Joshua R. Hyman ◽  
Jessica Gaus ◽  
Majid R. Foolad
Keyword(s):  
Total Variation ◽  
High Performance ◽  
Crop Improvement ◽  
Accurate Method ◽  
Carotene Content ◽  
Require Time ◽  
Wide Range ◽  
Lycopene Content ◽  
Β Carotene ◽  
Tomato Genotypes

Lycopene is the red pigment and a major carotenoid in tomato (Lycopersicon esculentum Mill.) fruit. It is a potent natural antioxidant, and the focus of many tomato genetics and breeding programs. Crop improvement for increased fruit lycopene content requires a rapid and accurate method of lycopene quantification. Among the various available techniques, high-performance liquid chromatography (HPLC) can be accurate, however, it is laborious and requires skilled labor and the use of highly toxic solvents. Similarly, spectrophotometric methods, although easier than HPLC, also require time-consuming extractions and may not be as accurate as HPLC, as they often overestimate fruit lycopene content. Colorimetric estimation of fruit lycopene using chromaticity values has been proposed as an alternative rapid method. Previous studies that examined the utility of this technique, however, were confined to the evaluation of only one or few cultivars and, therefore, lacked broad applicability. The purpose of the present study was to examine the utility of chromaticity values for estimating lycopene and β-carotene contents in tomato across diverse genetic backgrounds. Measurements of the chromaticity values (L*, a*, b*, C*, h*) were taken on whole fruit and purée of 24 tomato genotypes and were compared with HPLC measurements of fruit lycopene and β-carotene. Examination of different regression models indicated that a model based on the transformed value a*4 from purée measurements explained up to 94.5% of the total variation in fruit lycopene content as measured by HPLC. When this model was applied to a second set of fruit harvested at a later date from the same 24 genotypes, it explained more than 90% of the total variation in lycopene, suggesting its reliability. The best estimation for β-carotene content was obtained by using the b* chromaticity value from whole fruit measurements or the transformed a*2 value from purée measurements. Neither model, however, could explain more than 55% of the variation in β-carotene content, suggesting that chromaticity values may not be appropriate for estimating tomato β-carotene content. The overall results indicated that fruit lycopene content could be measured simply and rather accurately across a wide range of tomato genotypes using chromaticity values taken on fruit purée.

β-Carotene stimulates chemotaxis of human endothelial progenitor cells

2005 ◽  
Vol 43 (5) ◽  
Author(s):  
Beata Kieć-Wilk ◽  
Anna Polus ◽  
Joanna Grzybowska ◽  
Magdalena Mikołajczyk ◽  
Jadwiga Hartwich ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Hplc Method ◽  
Human Umbilical Cord ◽  
Cell Surface Antigens ◽  
Endothelial Progenitor ◽  
Expression Of Genes ◽  
First Time ◽  
Β Carotene

AbstractAngiogenesis is a crucial process in tissue remodeling during growth, both in the embryo and the adult. In our study we concentrated on the direct effect of β-carotene on human umbilical cord originating from endothelial progenitor cells (EPCs). β-Carotene uptake by EPCs was measured using a HPLC method. The determination of cell surface antigens was performed by flow cytometry. The effect on cell proliferation was estimated by measuring bromo-deoxyuridine incorporation. The influence on the formation of a tubular-like structure was investigated in a 3D assay in matrigel. Quantitative gene expression was estimated using real-time PCR. We demonstrated that β-carotene in the physiological range of concentrations found in human blood is a potent activator of EPC chemotaxis, which is accompanied by a change in the expression of genes mediating cell adhesion and homing, but does not activate the final markers of endothelial differentiation. This study points to the prochemotactic and homing activity of β-carotene in undifferentiated endothelial cell progenitors for the first time, which may suggest a potential role of this carotenoid in progenitor cell therapy aimed at angiogenesis and tissue repair.

scholarly journals Determination of β-Carotene in Commercial Foods: Interlaboratory Study

1997 ◽  
Vol 80 (5) ◽  
pp. 1057-1064 ◽  
Author(s):  
Willy Schuep ◽  
Joseph Schierle
Keyword(s):  
Sample Preparation ◽  
Collaborative Study ◽  
The Other ◽  
High Quality ◽  
Carotene Content ◽  
Relative Standard ◽  
Simple Extraction ◽  
Β Carotene ◽  
Significant Underestimation

Abstract Two sample preparation procedures for determination of total β-carotene and (all-)-β-carotene were tested in a collaborative study involving 14 laboratories and using 4 commercial products containing supplemented or natural β-carotene. One assay used classical sample preparation, consisting of saponification, extraction, washing, and drying ofthe extract and redissolution of residue for liquid chromatography (LC). The other assay used simple extraction without saponification. LC conditions were left for the analysts to decide as long as aclear separation of lycopene and ϑ-carotene from Bcarotene was achieved. Mean contents of test samples ranged from 0.3 to 18 mg/100 g for total β-carotene and from 0.2 to 16 mg/100 g for (all-)-β-carotene. Repeatability relative standard deviations (RSDr)for total B-carotene ranged from 2.9 to 5.6% and relative reproducibility standard deviation (RSDR) ranged from 6.5 to 15%. Corresponding values for(all-)-β-carotene were 3.3-5.1 % for RSDr and 8.4- 14% for RSDR. Excluding the (Z)-isomers from uantitation of β-carotene can result in significant underestimation of the effective β-carotene content especially if the LC systems used is high quality.

scholarly journals Definition and Analysis of the Main Requirements for Laboratory Competence

2020 ◽  
pp. 68-71
Author(s):  
О. А. Nykytyuk ◽  
V. М. Novikov
Keyword(s):  
International Organizations ◽  
Management System ◽  
Internal Audit ◽  
Technical Regulation ◽  
Laboratory Staff ◽  
Management Personnel ◽  
National Body ◽  
Wide Range ◽  
First Time

The work is devoted to the study of the actual issue of technical regulation and metrology — determination of criteria of competence of laboratories. The results of the worldwide accreditation work are subjective in nature and therefore require continuous improvement in the assessment methods used to determine the compliance of laboratories with standard competency requirements. The analysis of the main elements of the management system, which, in accordance with the Paretto principle, mainly form the competence of laboratories in its accreditation in the national body was held. For the first time, the authors, with the involvement of all stakeholders, have analyzed and identified major competency requirements for laboratories across a wide range of DSTU ISO / IEC 17025: 2017 requirements. Also, for the first time, the technology of peer review was applied to the analysis of the criteria of competence of laboratories. The recommendations of ILAC, EA and EUROLAB international organizations for the criteria of competence of laboratories are analyzed in detail. Particular attention is given to the introduction of specific requirements for staff competence and accreditation criteria, which are in one way or another related to the competence of the laboratory staff. It has been established experimentally (by the method of expert evaluation) that such elements of the management system as risk management, personnel management, internal audit, intralaboratory control and interlaboratory comparisons are considered today to be the most important in confirming the competence of a laboratory.

scholarly journals A stokes polarimetric light microscopy view of liquid crystal droplets

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
J. Gou ◽  
T. H. Shen ◽  
P. Bao ◽  
J. L. Ramos Angulo ◽  
S. D. Evans
Keyword(s):  
Liquid Crystal ◽  
Stokes Parameters ◽  
Full Description ◽  
Research Fields ◽  
Photoelastic Modulator ◽  
Internal Structures ◽  
Wide Range ◽  
Potential Applications

AbstractThe optical characteristics of materials, such as their magnetooptical effects, birefringence, optical activities, linear and circular dichroism, are probed via the polarisation states of light transmitted through or reflected from the specimens. As such, the measurements of the polarisation states play an important role in many research disciplines. Experimentally, Stokes parameters provide a full description of the polarisation states of light. We report the implementation of a dual- photoelastic modulator based polarimeter in a light microscope, enabling the determination of Stokes parameters at each pixel. As a case study, polarimetric images of liquid crystal droplets of different internal structures are obtained, showing their distinct polarisation characteristics. We demonstrate that the prototype Stokes polarimetric microscope allows the quantitative determination of the polarisation characteristics of light at the object plane and enables the access of the information of full polarisation states as compared to a conventional cross polariser microscope. This work shows that Stokes polarimetric microscopy may find potential applications in a wide range of research fields.

Development and Validation of an UV-Visible Method for the Determination of β-Carotene in the Leafy Vegetables in Thailand

2014 ◽  
Vol 1033-1034 ◽  
pp. 652-657
Author(s):  
Napaporn Rattanat ◽  
Kanjana Yodmora
Keyword(s):  
Chinese Cabbage ◽  
Storage Temperature ◽  
Morning Glory ◽  
Leafy Vegetables ◽  
Limit Of Quantification ◽  
Carotene Content ◽  
Relative Standard ◽  
Uv Visible ◽  
Β Carotene

Determination of β-carotene content in four types of leafy vegetables, water morning glory, kale, lettuce and Chinese cabbage by using a simple, rapid and low cost of UV-Visible method. This study was in the concentration range of 0.5 to 12 mg/L, and showed the linear regression equation y = 0.0764x - 0.0008, and an excellent linearity with correlation coefficient (R2) was 0.9999. The accuracy of the method analysis was reported by percentage recovery of about 80-106%. The precision was reported by percent of relative standard deviation and varies for intra-day 3.92% and inter-day 3.99%. The limits of detection and limit of quantification were 0.004 and 0.013 mg/L, respectively. Acetone is the most suitable for extraction, ratio of weight of samples to solvent, and extraction time was 1:3 g/mL and 10 min. Moreover, the most appropriate storage temperature for leafy vegetable samples is 4°C. Evaluation of β-carotene content under the suitable conditions in the kale, Chinese cabbage, lettuce and water morning glory was found to be 27.96 ± 2.99, 19.35 ± 1.92, 18.78 ± 0.88 and 18.48 ± 1.86 mg/100 g, respectively.

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