scholarly journals Analisis Senyawa β-Karoten pada Buah Pepaya (Carica papaya L.) Asal Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara

2021 ◽  
Vol 4 (2) ◽  
pp. 1-7
Author(s):  
Irman Idrus ◽  
Sabda Wahab ◽  
Andi Fitrah Nugraha ◽  
Syaiful Bachri
Keyword(s):  
Quantitative Analysis ◽  
The Body ◽  
Test Results ◽  
Carotene Content ◽  
Test Parameter ◽  
Qualitative Test ◽  
Uv Visible ◽  
Planting Method ◽  
Β Carotene

South Konawe District at Southeast Sulawesi Province is a producer of agriculture such as Papaya fruit ranging from 1168 quintals each year. Papaya fruit contains β-carotene which plays an important role in the formation of vitamin A in the body. This research was conducted to determine the β-carotene content of papaya fruit by using variables planting method, fruitage, and fruit condition. The mashed fruit was extracted using n-hexane: acetone: ethanol (2: 1: 1) v/v, then separated from polar and non-polar solutions. Qualitative analysis was carried out using the Carr-Price method and quantitative analysis or determination of β-carotene levels using the UV-Visible spectrophotometric method λ = 452.0 nm. The qualitative test results showed that the papaya fruit in the papaya fruit obtained by cultivation and growing wild with each variable half-ripe, ripe, and not fresh papaya identified the presence of β-carotene. The results of quantitative analysis of β-carotene levels in cultivated papaya fruit were 1.76 μL, half-ripe 1.32 μL, and cultivated young papaya 1.22 μL. In wild ripe papaya 1.75 μL, wild half-ripe papaya 1.3 μL and wild young papaya 1.21 μL. There is an effect of each test parameter starting from the parameters of planting method, fruitage, and fruit condition on β-carotene content in papaya fruit.

scholarly journals PENETAPAN KADAR VITAMIN C PADA KULIT PISANG (Musa paradisiaca) DENGAN METODE SPEKTROFOTOMETRI UV-VISIBLE TAHUN 2018

2020 ◽  
Vol 2 (2) ◽  
pp. 56-62
Author(s):  
Asti Pratiwi ◽  
Ardita Febrianty Manurung ◽  
Jhoti Sumitra
Keyword(s):  
Vitamin C ◽  
The Body ◽  
Water Soluble ◽  
Banana Peel ◽  
Research Use ◽  
Musa Paradisiaca ◽  
Qualitative Test ◽  
Uv Visible ◽  
Research Stage

Background: Vitamin C is a vitamin that is water soluble qnd one vitamin that are needed by the body. To meet the intake of vitamin C needed source of vitamin a derived from fruits, one of which is banana. Part of the banana , which is still rarely used is its skin. Banana peel is very beneficial for health however the situation is only considered as waste that has not been utilized well. This study aims to determine the levels of vitamin C on the skin of the banana (Musa paradisiaca) by the method of spectrofotometry uv-visible wavelength 224,5 nm. Method: This research use experimental with purely research stage comprise sample preparation, qualitative test, making the solution of the parent raw, quantitative test, determination of wavelength of maximum vitamin and creation of calibration surve. Result: Based on the research that has been done obtained the result of the qualitative test to prove the presence of high content of vitamin C contained in banana peels, with the highest level obtained in the skin of the plantain by 0,0253 mg/ml and the levels of the lowest on a banana peel the wax by 0,0172 ng/ml. Discussion: Levels of vitamin C on the skin of the banana  barangan of 0, 0219 mg/ml, sample a banana skin banten by 0, 0173 mg/ml, sample a banana peel wax by 0, 0172 mg/ml and sample the skin of plantain by 0, 0253 mg/ml.

Development and Validation of an UV-Visible Method for the Determination of β-Carotene in the Leafy Vegetables in Thailand

2014 ◽  
Vol 1033-1034 ◽  
pp. 652-657
Author(s):  
Napaporn Rattanat ◽  
Kanjana Yodmora
Keyword(s):  
Chinese Cabbage ◽  
Storage Temperature ◽  
Morning Glory ◽  
Leafy Vegetables ◽  
Limit Of Quantification ◽  
Carotene Content ◽  
Relative Standard ◽  
Uv Visible ◽  
Β Carotene

Determination of β-carotene content in four types of leafy vegetables, water morning glory, kale, lettuce and Chinese cabbage by using a simple, rapid and low cost of UV-Visible method. This study was in the concentration range of 0.5 to 12 mg/L, and showed the linear regression equation y = 0.0764x - 0.0008, and an excellent linearity with correlation coefficient (R2) was 0.9999. The accuracy of the method analysis was reported by percentage recovery of about 80-106%. The precision was reported by percent of relative standard deviation and varies for intra-day 3.92% and inter-day 3.99%. The limits of detection and limit of quantification were 0.004 and 0.013 mg/L, respectively. Acetone is the most suitable for extraction, ratio of weight of samples to solvent, and extraction time was 1:3 g/mL and 10 min. Moreover, the most appropriate storage temperature for leafy vegetable samples is 4°C. Evaluation of β-carotene content under the suitable conditions in the kale, Chinese cabbage, lettuce and water morning glory was found to be 27.96 ± 2.99, 19.35 ± 1.92, 18.78 ± 0.88 and 18.48 ± 1.86 mg/100 g, respectively.

Penetapan Kadar Senyawa β-Karoten Dalam Buah Semangka Kuning (Citrullus vulgaris Schrad) Dengan Metode Spektrofotometri UV-Vis

2021 ◽  
Vol 1 ◽  
pp. 351-357
Author(s):  
Maya Nisrina Huwaida ◽  
W Wirasti ◽  
S Slamet ◽  
Achmad Vandian Nur
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Test Results ◽  
Natural Pigment ◽  
Carotene Content ◽  
Citrullus Vulgaris ◽  
Good For ◽  
Β Carotene

AbstractWatermelon is a fruit that favored by the peoples because it tastes sweet and is good for health, watermelon also contains β-carotene. β-carotene is a natural pigment that is found in large amounts in red-orange fruits, including yellow watermelons. The purpose of this study was to determine β-carotene compounds and β-carotene levels in yellow watermelon flesh. The method used in this study for qualitative analysis using TLC method with chloroform: ethyl acetate (5: 5) mobile phase and for quantitative analysis using UV-Vis Spectrophotometry method. The test results showed that the yellow watermelon flesh sample contained β-carotene with a maximum wavelength of 458 nm and the β-carotene content of the yellow watermelon flesh was 146,253 µg/100 grams. Levels of β-carotene compounds in yellow watermelon flesh are included in the low category.Keywords: Yellow watermelon, β-carotene, TLC, UV-Vis spectrophotometry AbstrakSemangka merupakan buah yang banyak digemari oleh masyarakat karena rasanya manis dan baik bagi kesehatan, juga memiliki kandungan β-karoten. β-karoten adalah pigmen alami yang terdapat dalam jumlah besar pada buah-buahan yang berwarna merah-orange termasuk pada buah semangka kuning. Tujuan peneltian ini adalah untuk mengetahui senyawa β-karoten dan kadar β-karoten pada daging buah semangka kuning. Metode yang digunakan dalam penelitian ini untuk analisis kualitatif menggunakan metode KLT dengan fase gerak kloroform : etil asetat (5 : 5) dan untuk analisis kuantitatif menggunakan metode Spektrofotometri UV-Vis. Hasil pengujian menunjukkan sampel daging buah semangka kuning megandung β-karoten dengan Panjang gelombang maksimum yaitu 458 nm dengan kadar β-karoten pada daging buah semangka kuning yaitu 146,253 µg/100 gram. Kadar senyawa β-karoten dalam daging buah semangka kuning termasuk dalam kategori rendah.Kata kunci: Semangka kuning; β-karoten; KLT; spektrofotometri UV-Vis

scholarly journals Penentuan β-karoten dalam buah wortel (Daucus Carota) secara kromatografi cair kinerja tinggi (U-HPLC)

2019 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Sonlimar Mangunsong ◽  
Rifqi Assiddiqy ◽  
Eka Puspa Sari ◽  
Priscila Natalia Marpaung ◽  
Rahma Arum Sari
Keyword(s):  
Free Radicals ◽  
Daucus Carota ◽  
High Performance ◽  
Calcium Salt ◽  
Beta Carotene ◽  
The Body ◽  
Organic Chemical ◽  
Chloroform Methanol ◽  
Β Carotene

The lifestyle of people who tend to be unhealthy causes a lot of free radicals in the body which can cause various diseases, especially degenerative diseases. To protect the body from attacks by free radicals, the body needs antioxidants such as β-carotene. One of the vegetables containing β-carotene is “wortel” Daucus carota, that  is very large in agriculture. The spread of beta carotene from carrots with chemical solvents has been done a lot, but without chemical solvents it has never been done. Based on the color, the content of β-carotene contained in carrots determines the content of beta carotene. The juice obtained by calcium salt was then centrifuged for 3000 rpm 15 minutes. The pellets are separated from the solution, evaporated to dryness, measured at a wavelength of 450-460. Pellets as beta-carotene calcium salts were analyzed by U-HPLC. The next pellet is measured at 460 wavelengths. The levels are determined by high performance liquid chromatography using C18 column and the mobile phase of chloroform-methanol (95:5) with a flow rate of 1 ml / minute at a wavelength of 460 nm. The content of β-carotene in the examination is up to 92,5%. The retention time obtained is 1,903 minutes. The results showed that this method can be used for the withdrawal and determination of β-carotene levels without organic chemical solvents.Pola  hidup masyarakat yang cenderung tidak sehat menyebabkan  banyak radikal bebas di dalam tubuh yang dapat mengakibatkan berbagai penyakit terutama penyakit degeneratif. Perlindungan tubuh dari serangan radikal bebas, perlu antioksidan seperti β-karoten. Salah satu sayuran  yang mengandung β-karoten adalah wortel, jumlahnya sangat banyak dalam pertanian. Berdasarkan warnanya, maka kandungan β-karoten yang terdapat dalam wortel menjadi penentu  kandungan beta karoten Metode ektraksi yang aman dari bahan pelarut kimia mutlak diperlukan.  Untuk mengetahui kandungan β-karoten dalam wortel,  lebih  dahulu dilakukan penghalusan  dengan blender, kemudian sari difilter /dipisahkan dari ampasnya. Sari  yangdiperleh ditambahkan garam kalsium kemudian disentrifus 3000 rpm  15 menit. Pelet dipisahkan dari larutannya, diuapkan hingga kering, Diukur pada panjang gelombang 450-460. Pelet sebagai garam kalsium betakaroten dianalisa dengan U-HPLC.  Pelet selanjutkan diukur pada panjang gelombang 460. ditetapkan kadarnya secara kromatografi cair kinerja tinggi menggunakan kolom C18 dan fase gerak kloroform–metanol (95:5) dengan laju alir 1 ml/menit pada panjang gelombang 460 nm. Kandungan β-karoten dalam pemeriksaan  sampai 92,5%. Waktu retensi yang diperoleh adalah 1,903 menit.  Hasil penelitian menunjukkan bahwa metode ini dapat digunakan untuk penarikan dan penetapan kadar β-karoten dalam   tanpa pelarut kimia organik.

scholarly journals IDENTIFIKASI KADAR VITAMIN C PADA DAGING DAN KULIT BUAH NAGA MERAH (Hylocereus Polyrhizus) DENGAN METODE SPEKTROFOTOMETRI UV-VISIBLE

2019 ◽  
Vol 2 (1) ◽  
pp. 40-46
Author(s):  
Aminah Nur Syarifuddin ◽  
Rezky Zantrie ◽  
Romauli Anna Teresia Marbun
Keyword(s):  
Free Radicals ◽  
Vitamin C ◽  
Calibration Curve ◽  
Raw Materials ◽  
Quantitative Test ◽  
Dragon Fruit ◽  
Qualitative Test ◽  
Uv Visible ◽  
Hylocereus Polyrhizus

Introduction: Vitamin C is one of the nutrition acted as a antioxidants and effective addres free radicals can be destructive cells or tissue. Fruits are a source of vitamins C, which is a dragon red. Part of the dragon red during this rarely used is the skin. The dragon fruit is very useful to health, but in fact only thought of as waste farming has yet to be used as a good. This study aims to determine comparison levels of vitamin C on the flesh and the dragon red (Hylocereus polyrhizus) with a method spectrophotometry UV-Visible. Method: This type of research using the method of true experimental reseach stage includes preparation of samples, qualitative test,quantitative test, making the solution of the parent raw materials, determination of wavelength of vitamin C and manufacture of the calibration curve. The sample of this study is the flesh and the dragon red (Hylocereus polyrhizus). Results: Based on the results revealed that the fles and the dragon red (Hylocereus polyrhizus) contain vitamin C. Discussion: Levels of vitamin C on the flesh the dragon red of 0.05888 mg/mL and The levels of vitamin C on the skin the dragon red of 0.02533 mg/mL .

scholarly journals ANALISIS KADAR TOTAL METABOLIT SEKUNDER EKSTRAK ETANOL DAUN TAMOENJU (Hibiscus surattensis L.)

KOVALEN ◽  
2017 ◽  
Vol 3 (3) ◽  
pp. 258
Author(s):  
Astrid Natalia Alasa ◽  
Syariful Anam ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Gravimetric Analysis ◽  
Test Results ◽  
Quantitative Test ◽  
Test Parameters ◽  
Qualitative Test ◽  
Uv Visible ◽  
Dark Blue

Tamoenju (Hibiscus surattensis L.) leaves are included in the malvaceae family which grows in the plateau and are known to have the antidiabetic activity that the standardization of the extract is needed to be conducted. The research aimed to discover the total level of secondary metabolites from ethanol extract. The extraction of Tamoenju leaves through maceration method with 96% ethanol was done to obtain the filtrate. The filtrate obtained was concentrated by using rotary evaporator at 40°C until the concentrated extract was obtained. The extract gained was then tested in a qualitative way with Alkaloid, Flavonoid, Saponin, and Tannin tests by using the suitable reagent for the test parameters. While in the quantitative test the method used was Gravimetric analysis on alkaloid and Saponin compounds, Permanganometry on Tannin compound, and UV-Visible Spectrophotometry on Flavonoid. The qualitative test results reveal that Tamoenju is positive for containing Alkaloid which was characterized by the presence of orange sediment, Flavonoid was characterized by the orange formation, Tannin was characterized by the dark blue and Saponin was characterized by the stable foam. The quantitative test results are Alkaloid by 305.181 g/g, Saponin by 371.112 g/g, Tannin by 55.417 g/g and Flavonoid by 14.999 mg/100 g.Keywords: Hibiscus surattensis L. , secondary metabolites, standardization

Reaction Chamber and Cathode Configurations in Arc Production of Fullerenes

1994 ◽  
Vol 359 ◽  
Author(s):  
E. Pasqualini ◽  
C. Podesta ◽  
A. GarcÍa ◽  
A. Rafael ◽  
S. Dengra ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Reaction Chamber ◽  
Decomposition Rates ◽  
Deposit Formation ◽  
Cathodic Deposit ◽  
Visible Spectra ◽  
Uv Visible ◽  
Different Origins ◽  
Equivalent Conditions

ABSTRACTThe reaction chamber in the arc production of fullerenes was redesigned with a nozzle surrounding the decomposition zone to allow for clean collection of soot in a filtering cartridge. Quantitative analysis in the region of 300–430 nm in UV-visible spectra permits determination of the abundance of C60 and C70 in the soot. Calibrated curves of absorptivity for both pure fullerenes were employed. In equivalent conditions of current and pressure, electrographites of different origins have different decomposition rates and yields. A mechanism to interpret the cathodic deposit formation is proposed. Decomposition inside a closed cathodic cylinder yields 100% deposit.

scholarly journals Determination of β-Carotene in Commercial Foods: Interlaboratory Study

1997 ◽  
Vol 80 (5) ◽  
pp. 1057-1064 ◽  
Author(s):  
Willy Schuep ◽  
Joseph Schierle
Keyword(s):  
Sample Preparation ◽  
Collaborative Study ◽  
The Other ◽  
High Quality ◽  
Carotene Content ◽  
Relative Standard ◽  
Simple Extraction ◽  
Β Carotene ◽  
Significant Underestimation

Abstract Two sample preparation procedures for determination of total β-carotene and (all-)-β-carotene were tested in a collaborative study involving 14 laboratories and using 4 commercial products containing supplemented or natural β-carotene. One assay used classical sample preparation, consisting of saponification, extraction, washing, and drying ofthe extract and redissolution of residue for liquid chromatography (LC). The other assay used simple extraction without saponification. LC conditions were left for the analysts to decide as long as aclear separation of lycopene and ϑ-carotene from Bcarotene was achieved. Mean contents of test samples ranged from 0.3 to 18 mg/100 g for total β-carotene and from 0.2 to 16 mg/100 g for (all-)-β-carotene. Repeatability relative standard deviations (RSDr)for total B-carotene ranged from 2.9 to 5.6% and relative reproducibility standard deviation (RSDR) ranged from 6.5 to 15%. Corresponding values for(all-)-β-carotene were 3.3-5.1 % for RSDr and 8.4- 14% for RSDR. Excluding the (Z)-isomers from uantitation of β-carotene can result in significant underestimation of the effective β-carotene content especially if the LC systems used is high quality.

scholarly journals “SUBSTITUSI TEPUNG Cucurbita moschata (Duch.) Poir TERHADAP KADAR β-KAROTEN DAN DAYA TERIMA CAKE LABU KUNING”

2021 ◽  
Vol 4 (1) ◽  
pp. 7-15
Author(s):  
Frederika Pellu Dimu ◽  
Andriani Rafael ◽  
Sonya T.M Nge
Keyword(s):  
Cucurbita Moschata ◽  
Test Results ◽  
Alternative Source ◽  
Carotene Content ◽  
Tukey Test ◽  
A Value ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Collection Methods ◽  
Β Carotene

ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-carotene as a precursor of vitamin A and has a fairly high carbohydrate content. Pumpkin processing can be done to produce various kinds of products, one of which is cake. Cake is a baked dough and liquid dough made from flour, sugar, salt, ingredients, shortening, milk, eggs, and aroma enhancer. This study aims to determine the levels of β-carotene contained in pumpkin cake and to determine the acceptability of β-carotene in pumpkin cake. This research is an experimental research. The experimental design used was Completely Randomized Design (CRD) with 1 control 4 treatments and each treatment was repeated 3 times. The research phase consisted of making flour and making cake. Data collection methods include organoleptic tests and β-carotene tests. Data were analyzed using analysis of variance / ANOVA to determine differences in quality, and if there is an influence then proceed with the Tukey test. Organoleptic test results show that the best treatment of pumpkin cake in terms of color is A1 with a value of 3.88, in terms of aroma namely A0 with a value of 3.92, in terms of texture is A0 with a value of 4.28 and in terms of taste is A0 with value of 4.16. As for the β-carotene test, the treatment of pumpkin cake with the highest β-carotene content was found in A4 with an average of 0.262 and the lowest level was in A0 with an average of 0.018. Based on research data, pumpkin flour is worthy of being used as an alternative source of flour because the resulting cake meets SNI quality standards both in terms of color, taste, aroma, texture and β-carotene content.Keywords : Cake, Pumpkin, β-Carotene, Organoleptic

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